Volume 4 Issue 1最新文献_第4页

L-Carnitine Improves Cytoprotection during Cryopreservation: A case study on Nili-Ravi Buffalo Sperm 左旋肉碱在低温保存过程中增强细胞保护作用:以Nili-Ravi水牛精子为例

Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.3.4.10

S. Qadeer, R. Ejaz, A. Husna, A. Azam, S. Rubab, G. Nabi, S. Ullah, S. Akhter

{"title":"L-Carnitine Improves Cytoprotection during Cryopreservation: A case study on Nili-Ravi Buffalo Sperm","authors":"S. Qadeer, R. Ejaz, A. Husna, A. Azam, S. Rubab, G. Nabi, S. Ullah, S. Akhter","doi":"10.34091/ajls.3.4.10","DOIUrl":"https://doi.org/10.34091/ajls.3.4.10","url":null,"abstract":"The current study was aimed to evaluate the antioxidative effect of L-Carnitine at post thawing following cryopreservation of Nili-Ravi buffalo sperm. For this purpose, semen from three buffalo bulls were collected for 3 weeks using an artificial vagina (N=18; replicates). The qualified ejaculates were diluted employing tris-citric acid extender i.e., control did not receive any L-Carnitine and experimental groups having 0.5, 1.0, and 1.5 ng/mL of L-carnitine at 37 C with approximately 50 x 106 sperm/mL. The semen was cooled at 4 C and then equilibrated (4 hours), filled in straws (0.5 mL) at4 C, placed on LN2 vapours for 10 min, and kept into an LN2 container. The thawed semen was evaluated for post-thaw quality. The integrity of the sperm plasma membrane and motility (P?0.05) was highest in the extenders having 1.0 ng/mL of L-carnitine as compared to the control(received no L-Carnitine). However, sperm chromatin integrity and viability(live sperm with intact acrosome) remained similar. It was concluded that supplementing 1.0 ng/mL L-Carnitine of extender can improve the post-thaw quality of cryopreserved sperm. Based on the results of the current experiments it is recommended to include L-carnitine extender to improve post-thaw quality of buffalo sperm in terms of its motility and integrity ofits plasma membrane.\u0000\u0000Keywords: Buffalo, Sperm, Cryopreservation, Extender, L-Carnitine, Artificial insemination.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91018936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the genetic divergence and phenotypic association in elite Pakistani wheat (Triticum aestivum L.) genotypes 揭示优质巴基斯坦小麦(Triticum aestivum L.)基因型的遗传分化和表型关联

Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.14

N. Gandahi, A. W. Baloch, Mir Yar Mohammad Talpur, N. Fatima, T. F. Abro, G. H. Jatoi, Asadullah Mari, Liaquat Ali Bhutto, Rongshu Zhang

{"title":"Unveiling the genetic divergence and phenotypic association in elite Pakistani wheat (Triticum aestivum L.) genotypes","authors":"N. Gandahi, A. W. Baloch, Mir Yar Mohammad Talpur, N. Fatima, T. F. Abro, G. H. Jatoi, Asadullah Mari, Liaquat Ali Bhutto, Rongshu Zhang","doi":"10.34091/ajls.4.1.14","DOIUrl":"https://doi.org/10.34091/ajls.4.1.14","url":null,"abstract":"The present study was designed to assess genetic divergence between commercial bread wheat genotypes based on yield and its related traits and to carry out phenotypic correlation. Twenty bread hexaploid wheat varieties were assessed for mean performance, correlation analysis and genetic distance. Randomized complete block design was used with three replications during growing season, 2018-2019 at Wheat and Barley Research Institute, Tandojam. The mean squares depicted significant differences (P<0.01) for all the investigated traits among the tested genotypes, proving that used genetic resources possess a great potential for further breeding experiments. Regarding mean performance, the genotypes Sassui and TD-1 displayed desirable performance for a variety of traits, unveiling their importance in wheat breeding programs. The results also showed that tillers plant-1, grains spike-1 and seed index developed significantly positive (P<0.05) interrelationship with grain yield plant-1. This demonstrates that genotypes possessing higher extent of these traits may be chosen in selection for developing high yielding bread wheat genotypes. The larger genetic distance was witnessed between Johar-78 and TD-1, followed by SKD-1 and TJ-83, SKD-1 and Mehran-89, SKD-1 and Johar-78, TD-1 and Khirman, TJ-83 and Imdad-05, Mehran-89 and TD-1 and Johar-78 and TJ-83. These paired showed wide genetic distances, which may also be preferred in wheat hybridization program.\u0000\u0000Keywords: Genetic distance, hexaploid wheat, morphological traits, trait","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79051766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Staphylococcus aureus hemolysin toxins on blood cells and association with skin and soft tissue infections 金黄色葡萄球菌溶血素毒素对血细胞的影响及其与皮肤和软组织感染的关系

Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.18

Adnan Shahid, Afsheen Rafiq

{"title":"Effects of Staphylococcus aureus hemolysin toxins on blood cells and association with skin and soft tissue infections","authors":"Adnan Shahid, Afsheen Rafiq","doi":"10.34091/ajls.4.1.18","DOIUrl":"https://doi.org/10.34091/ajls.4.1.18","url":null,"abstract":"Staphylococcus aureus (S. aureus) is gram positive, catalase positive cocci which belongs to the family of Staphylococcaceae and is long known as clinical and foodborne pathogen. The emergence of multidrug resistance strain of S. aureus which is methicillin resistant S. aureus (MRSA) challenges the health care system because it can cause wide variety of hospital and community acquired skin and soft tissue infections which are difficult to treat. The virulence of S. aureus is because of different factors which includes toxins, enzymes and superantigens. S. aureus produce variety of exotoxins, enterotoxins and exfoliative toxins which contributes to the virulence of S. aureus. Hemolysin toxins produce by S. aureus strains are associated with different skin and soft tissue infections (SSTIs) and can cause the lysis of RBCs. Hemolysins are regulated by accessory gene regulator (agr) and is required for the enhanced expression of virulence factors secreted by S. aureus. Hemolysins have leucolytic activity and can help in iron scavenging from host. The most important toxin is alpha hemolysin which can induce the apoptosis and cause the lysis of epithelial cells, erythrocytes and keratinocytes. Human immune cells are affected by beta hemolysin and gamma hemolysin is a biocomponent toxin. Delta hemolysin is low molecular weight exotoxin which belongs to the class of phenol soluble modulins.\u0000\u0000Keywords: MRSA, Exotoxins, Hemolysins, SSTIs","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81987965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transferring of Lactobacillus antibiotic resistant genes to Salmonella 乳酸杆菌耐药基因向沙门氏菌转移

Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.17

Muhammad Ashraf, S. Rahman, Muhammad Jawad Bashir, R. Aslam, Sultan Ali, A. Jabbar

引用次数: 0

Trichogramma chilonis, an effective egg parasitoid for control of white stem borer Scirpophaga innotata (Walker), (Lepidoptera: Pyralidae) in rice 一种防治水稻白茎螟虫(鳞翅目:蚜科)的有效卵寄生蜂——黄颡鱼赤眼蜂

Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.6

Muhammad Yousif Rahimoon, Abdul Ghani Lanja, A. Bukero, Babar Hussain Chang, Jamal-u-ddin Hajano, M. McNeill, Ammara Rajput, Zainab Lanjar

{"title":"Trichogramma chilonis, an effective egg parasitoid for control of white stem borer Scirpophaga innotata (Walker), (Lepidoptera: Pyralidae) in rice","authors":"Muhammad Yousif Rahimoon, Abdul Ghani Lanja, A. Bukero, Babar Hussain Chang, Jamal-u-ddin Hajano, M. McNeill, Ammara Rajput, Zainab Lanjar","doi":"10.34091/ajls.4.1.6","DOIUrl":"https://doi.org/10.34091/ajls.4.1.6","url":null,"abstract":"Rice an important staple food crop of the world, which is attacked by the various insect pests, among them the stem borers are the most destructive pests, distributed in all rice growing areas of the world and cause the severe losses. This research investigated the efficacy of the parasitoid Trichogramma chilonis (Ishii) to control the white stem borer (Scirpophaga innotata) in rice. In this respect the field studies were conducted in lower Sindh, Pakistan for the year, Kharif - 2017 and 2018. Trichogramma egg parasitoid over 230 species are reported to have been remained in biological control of insect pests of over 200 insect species belonging to 70 different families, mainly the order Lepidoptera, Hemiptera, Orthroptera and Thysnoptera, in order to manage the white stem borer of rice the Trichogramma chilonis (Ishii) was used as a bio-control agent. The Studies revealed that T. chilonis proved an effective biocontrol strategy against white stem borer of rice. The result of this study could be useful in an integrated pest management program for the management of white stem borer of rice the Trichogramma chilonis.\u0000\u0000Keywords: Stem borer, Biocontrol, Parasitoid, Population suppression.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75210067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Diagnostic approaches and prevalence of Rifampicin resistant Mycobacterium tuberculosis in District Mardan 马尔丹区耐利福平结核分枝杆菌的诊断方法及流行情况

Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.8

Atif Kamil, Haseeb Ullah, Farooq Hussain, M. Jamal, Azmat Ali, M. Shah, Ayaz Ahmad, J. Khan, F. Anwar, Nabila

{"title":"Diagnostic approaches and prevalence of Rifampicin resistant Mycobacterium tuberculosis in District Mardan","authors":"Atif Kamil, Haseeb Ullah, Farooq Hussain, M. Jamal, Azmat Ali, M. Shah, Ayaz Ahmad, J. Khan, F. Anwar, Nabila","doi":"10.34091/ajls.4.1.8","DOIUrl":"https://doi.org/10.34091/ajls.4.1.8","url":null,"abstract":"The disease tuberculosis (TB) caused by Mycobacterium Tuberculosis (MTB) is most common infectious disease in developing countries. The disease is fatal if not treated during the early stages of infection, thereby early and precise detection is a decisive step in curing the disease. The aim of this study was to analyze the prevalence of tuberculosis in patients reporting to Mardan Medical Complex (MMC), located in the district Mardan, KPK, Pakistan. The sputum of patients was analysed by Ziehl-Nilsen (ZN) staining technique followed by light microscopy called Acid-Fast Bacillus (AFB) staining. The sputum samples were collected from the patients and analysed by special PCR method called GeneXpert MTB/RIF assay, for genomic detection and resistance assay for rifampicin antibiotic were used, are the commonly used medicine for the treatment of MTB infection. Total 121 patients reported to MMC, represented 74 % patients from Mardan, 12% from Nowshera and 14% from Swabi. These patients were screened for the aim to evaluate the techniques for the detection of MTB. The light microscopy method confirmed 66 (55%) of the patients positive for MTB, whereas the same samples reported 78 (68%) patients positive for MTB through GeneXpert MTB/RIF assay, The Positive Predictive Value (PPV) and Negative Predictive Value (NPV) found for light microscopy were 99% and 78.1% respectively. The most used drug rifampicin was found ineffective in 9 patients (7%). Additionally, 83% of the patients when interviewed had a folk history of tuberculosis.\u0000\u0000Keywords: Microscopy, PCR, Mycobacterium tuberculosis, Rifampicin.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85541021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell Transplantation Therapies to Reverse Type 1 Diabetes: A review 细胞移植治疗逆转1型糖尿病:综述

Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.12

Amrah Inam, Z. Alam, O. Shah, Misbah, F. Shi

{"title":"Cell Transplantation Therapies to Reverse Type 1 Diabetes: A review","authors":"Amrah Inam, Z. Alam, O. Shah, Misbah, F. Shi","doi":"10.34091/ajls.4.1.12","DOIUrl":"https://doi.org/10.34091/ajls.4.1.12","url":null,"abstract":"Stem cell technology is demonstrating promising advancements in cure of diseases due its differentiation ability. Type 1 diabetes is mainly caused by autoimmune ? cells destruction. In this review, we focus on treatment procedures of Type 1 Diabetes (T1D) with numerous stem cells (SCs) i.e hPSCs, MSCs, hESCs, BMSCs, AFSCs, HSCs and islet cells (that are not stem cells but they are approved worldwide and are being successfully used to permanently reverse T1D). A brief overview of this disease along with the advancements in treatment of T1D with stem cells is discussed. Biomaterial encapsulation to avoid immune rejection and improved immunomodulation and immune tolerance via drugs /bioengineering techniques makes the outcomes of SC therapies more efficient and productive, hence, proving to be another future milestone of completely reversing type 1 diabetes especially in those patients who got clinically diagnosed at an early stage and then received prompt treatment of either restoration of already available ? cells functionality or transplantation of purified and functional SCs differentiated insulin producing cells to normalize the glycemic control and homeostasis.\u0000\u0000Keywords: Type 1 Diabetes, ?-cells, Stem cells, Biomaterial","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88667037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Assessing Late Antique villa transformation at individual sites: towards a spatial approach 评估晚期古玩别墅在个别地点的改造:走向空间的方法

Volume 4 Issue 1 Pub Date : 2021-08-12 DOI: 10.16995/traj.4336

J.A.L.D. Dodd

引用次数: 0

TRAC at 30: A Bibliometric Analysis of the TRAC Community TRAC 30年:TRAC社区的文献计量学分析

Volume 4 Issue 1 Pub Date : 2021-02-24 DOI: 10.16995/TRAJ.4344

Nicky Garland

引用次数: 2

Finding the Social Life of Rural Non-Elites in Roman Italy 探寻罗马意大利乡村非精英阶层的社会生活

Volume 4 Issue 1 Pub Date : 2021-02-24 DOI: 10.16995/TRAJ.4345

Brian Theng

引用次数: 0