Volume 4 Issue 1最新文献

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Association between Interleukin-10 promoter polymorphism with type 2 Diabetes mellitus 白细胞介素-10启动子多态性与2型糖尿病的关系
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.4
Hafiz Umair Hassan, Rana Haider Ali, H. Siddiq, Fahad Ahmed, Hafiz Hanan Taj, Muhammad Farhan Rashid, Sami Ullah
{"title":"Association between Interleukin-10 promoter polymorphism with type 2 Diabetes mellitus","authors":"Hafiz Umair Hassan, Rana Haider Ali, H. Siddiq, Fahad Ahmed, Hafiz Hanan Taj, Muhammad Farhan Rashid, Sami Ullah","doi":"10.34091/ajls.4.1.4","DOIUrl":"https://doi.org/10.34091/ajls.4.1.4","url":null,"abstract":"Type 2 diabetes mellitus is a multifactorial illness triggered by a complicated interplay of various genetic variants with various environmental variables. The quantity of replicated common genetic variants associated with type 2 diabetes mellitus has increased rapidly with the recent genome-wide association (GWA) research. Major health issue in the public are type 2 diabetes mellitus (T2DM) is common throughout the world. Diabetes mellitus incidence is growing and is anticipated to affect 300 million individuals by 2025. Diabetes has been suggested to alter patterns of cytokine expression as an immune-dependent illness. Insulin resistance (IR) is a disease that results in less than anticipated biological impact of a specified insulin concentration. Insulin resistance and insulin secretion decreased are both defined pathophysiology of T2DM. One of the most alarming health issues of the 21st century is the spread of diabetes around the globe. Our goal in this study was to identify the role of IL-10 polymorphism in T2DM patients. The average age of 60 median patients with type 2 diabetes mellitus (31 males and 29 females) +-SD (45.91667 +-16.08799), fasting blood sugar (FBS) is +-SD (184.25 +-57.76387), hypertension (35 positive/25 negative) and 60 non-diabetic controls (32 males and 28 females) is +- SD (47.31667 +-15.13722). The group (T2DM patients and their control) had not a substantial distinction (P=0.33) and in each group (CC, TT, CT) there was a comparison between IL-10 gene polymorphism. T2DM patients and healthy individuals are not associated with the polymorphism of the gene IL-10 (SNP rs 3021097 (C/T).\u0000\u0000Keywords: Insulin, Diabetes Mellitis Type 2, Polymorphism, Interleukin-10, Genotype frequencies.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84586657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary intake to help prevent Covid-19 implications: The devil is in the detail 饮食摄入有助于预防Covid-19:细节决定成败
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.3
Sana Noreen, Abdul Rehman, R. Kanwal, S. Saleem, A. Sadiqa, Mudassir Khan
{"title":"Dietary intake to help prevent Covid-19 implications: The devil is in the detail","authors":"Sana Noreen, Abdul Rehman, R. Kanwal, S. Saleem, A. Sadiqa, Mudassir Khan","doi":"10.34091/ajls.4.1.3","DOIUrl":"https://doi.org/10.34091/ajls.4.1.3","url":null,"abstract":"One of the most frequent antecedents of mortality in world is coronavirus that proceeds multitude metabolic disorders and is consequence of damaged lungs that is ultimately caused by contagious infection and virus called coronavirus. It effects people of every age mostly dominating in child, aged, people who have already poor immune system and lungs related disorders. It has tremendous effects on body affecting physical, emotional, cognitive and intellectual abilities as well. It leads to various clinical moderate to severe complications including acute respiratory distress syndrome (ARDS), pneumonia, coughing up blood, persistent chest pain, fever, SOB (shortness of breath), coughing up sputum, chills, sore throat, respiratory sputum production (phlegm), loss of sense and smell, headache, muscle aches, rashes and diarrhea etc. One of the most imprudent factors examined in these patients is acute respiratory distress syndrome (ARDS) as well which refers to respiratory failure, a mechanism in which body is unable to respond normally sequentially results by high level of widespread inflammation in lungs. Penurious immune system which contributes to higher rate of mortality worldwide present strong association with COVID-19. Metabolic disturbances contribute to the development and progression of respiratory distress, as well as they have serious consequences in next stages of life that can have harmful effects on health. In this review we will discuss the pathophysiology as well as the signs and symptoms of COVID-19, the morbidities related to this disease and the treatment required controlling this disorder.\u0000\u0000Keywords: Covid-19, Diet, Health Benefits.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84524920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of nutritional amendments and fungicides against brown leaf spot of rice 营养改良剂和杀菌剂防治水稻褐叶斑病的效果评价
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.11
Fiza Mohsin, Safdar Ali, M. U. Ghani, W. Ashraf, M. Zeshan, R. Mahmood, Muhammad Muntazir, Mehdi Khan
{"title":"Evaluation of nutritional amendments and fungicides against brown leaf spot of rice","authors":"Fiza Mohsin, Safdar Ali, M. U. Ghani, W. Ashraf, M. Zeshan, R. Mahmood, Muhammad Muntazir, Mehdi Khan","doi":"10.34091/ajls.4.1.11","DOIUrl":"https://doi.org/10.34091/ajls.4.1.11","url":null,"abstract":"Helminthosporium oryzae, the causal organism of bacterial leaf spot (BLS) has brought a tremendous loss of rice crop in the field. Rice seedlings of four commercial varieties (Nayab basmati, Kiran 434, Super basmati, and Pak 386) were screened against BLS in the field by using a randomized complete block design (RCBD). The present experiment was encompassed the evaluation of fermented farmyard manure, leaf manure, Boron, and NPK solution against BLS. The effect of different fungicides (Score, Flumax, Melodyduo, and Polyram) was also checked on the mycelial growth of Helminthosporium oryzae in-vitro and in-vivo. The results showed that Pak 386 was highly resistant with 17.22% disease severity and Super Basmati was found moderately resistant with 22.31% disease severity; variety Kisan 434 was found moderately susceptible (24.15%) and Nayab Basmati (24.82%) was susceptible. Among all NPK solutions showed the best results in growth enhancement, plant vigor as well as reduction in disease severity followed by FFYM, boron and leaf manure. Under in-vitro conditions, all the fungicides performed best at 150 ppm in terms of colony growth reduction. The maximum growth inhibition was recorded in Score followed by Flumax, Melodyduo, and Polyram. Score gave a significant reduction in BLS disease severity and maximized the agronomic attributes. The fungicides performed best at 150 ppm in terms of colony growth reduction. The maximum growth inhibition was recorded in Score followed by Flumax, Melodyduo, and Polyram. Score gave a significant reduction in BLS disease severity and maximized the agronomic attributes.\u0000\u0000Keywords: HBV, HCV, ICT, PCR, Risk factors, Mansehra","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82635339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Purification of Thrombolytic Enzyme Extracted from Earthworm Punjab, Pakistan 旁遮普蚯蚓溶栓酶的分离纯化
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.15
B. Nasrullah, M. F. Malik, S. Afsheen
{"title":"Isolation and Purification of Thrombolytic Enzyme Extracted from Earthworm Punjab, Pakistan","authors":"B. Nasrullah, M. F. Malik, S. Afsheen","doi":"10.34091/ajls.4.1.15","DOIUrl":"https://doi.org/10.34091/ajls.4.1.15","url":null,"abstract":"The Cardiovascular disease due to thrombus (clot) formation is the major factor of death throughout the world. Earthworms being the eco engineers has thrombolytic enzyme that can be used for thrombolysis. The thrombolytic enzyme was isolated and purified from supernatant of earthworm Apporectodea longa by column chromatography. Six Strain BKT 11, BKT 15, BKT 17, BKT 26, BKT 27 and BKT 28 shows the thrombolytic activity 791.64 U/mg, 1362.39 U/mg, 1205.4 U/mg, 710.63 U/mg, 529.66 U/mg and 625.00 U/mg respectively. Thrombolytic activity was confirmed by blood clot lysis method. Different concentrations 50 ?l,100 ?l, 150 ?l, 200 ?l and 250 ?l of extracted enzyme were applied on 25mg of wet blood clot along with control where distill water used. These fractions of extracted enzymes represent the dissolution of clot (thrombolysis). The molecular weight 32 KDa was determined by sodium dodecyl sulphate gel electrophoresis (SDS-PAGE). Results show that extracted elute have potential of fibrinolytic activity in this specie of earthworm and it can serve as a suitable therapeutic agent.\u0000\u0000Keywords: Thrombolytic activity, Casein plate assay, Blood clot lysis, spectrophotometry, Gel electrophoresis.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79905697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial Susceptibility of Pseudomonas aeruginosa Isolated from Hospital Environment 医院环境中铜绿假单胞菌的药敏分析
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.5
Munzer Ullah, H. Ullah, Khaliq Noor, M. Sarfraz, Misbah Ullah Khan, U. Bibi, G. Nabi, Maheen Kanwal, Kainat Ramzan, A. Metwaly
{"title":"Antimicrobial Susceptibility of Pseudomonas aeruginosa Isolated from Hospital Environment","authors":"Munzer Ullah, H. Ullah, Khaliq Noor, M. Sarfraz, Misbah Ullah Khan, U. Bibi, G. Nabi, Maheen Kanwal, Kainat Ramzan, A. Metwaly","doi":"10.34091/ajls.4.1.5","DOIUrl":"https://doi.org/10.34091/ajls.4.1.5","url":null,"abstract":"Pseudomonas aeruginosa is a leading cause of disease and death particularly in cystic fibrosis patients and also considered resistance to chemotherapeutic agents. Therefore, it is very difficult to remove the Pseudomonas aeruginosa from the hospital environment by using simple techniques. In the contemporary study, biofilm mediated mechanism of various antimicrobial responses were analyzed. For this purpose, different Pseudomonas aeruginosa clinical isolates were collected from Pakistan medical institute Islamabad (PIMS) hospital and were investigated for pellicle formation. Pseudomonas aeruginosa isolates were studied for different groups of antibiotics including imipenem, meropenem, ceftazidime, amikacin, tobramycin, gentamicin, piperacillin, cefoperazone, and cefotaxime. The goal was to check antimicrobial susceptibility of pseudomonas aeruginosa which shows resistant to tobramycin, imipenem, meropenem, amikacin, gentamicin, cefotaxime, piperacillin, ceftazidime, cefoperazone. Additionally, in this study, Pseudomonas aeruginosa strains were also investigated for pellicle formation. In conclusion, this research work wills highlights the useful mechanism of antibiotics resistance to Pseudomonas aeruginosa infections in clinical practice.\u0000\u0000Keywords: Antibiotics, Pseudomonas aeruginosa, antibiotics, Biofilm, Peliclle.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"256 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79527325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of Vancomycin Resistance Determinants in Twin Cities of Rawalpindi/Islamabad 拉瓦尔品第/伊斯兰堡双城万古霉素耐药决定因素的鉴定
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.13
Sidra Rehmat Ullah, S. Andleeb, Taskeen Raza, K. Mehmood
{"title":"Identification of Vancomycin Resistance Determinants in Twin Cities of Rawalpindi/Islamabad","authors":"Sidra Rehmat Ullah, S. Andleeb, Taskeen Raza, K. Mehmood","doi":"10.34091/ajls.4.1.13","DOIUrl":"https://doi.org/10.34091/ajls.4.1.13","url":null,"abstract":"Vancomycin resistant Enterococci have emerged rapidly in the recent years leading to treatment failure. The aim of this work was to identify vancomycin resistance determinants; vanA and vanB genes in Enterococci. The blood, urine and throat samples were collected from 150 patients from local hospitals of twin cities of Rawalpindi/Islamabad. Forty-nine phenotypically confirmed isolates were further confirmed by PCR amplification for vancomycin determinants (genes for vanA and vanB) for vancomycin resistant Enterococci. Dependence and frequency distribution of VRE and VSE bacteraemia with respect to age, gender and source was also studied. Phenotypically resistant strains were positive for vanA while negative for vanB. vancomycin susceptible enterococci (VSE) could be isolated more from urine samples as compared to blood whereas vancomycin resistant enterococci (VRE) was found more prevalent in the blood samples (p value= 0.000). VRE was more frequently isolated from patients aged 50 or above whereas VSE prevalence was same in both age groups (p value=0.002). Gender was not found to have any significant impact on VRE or VSE bacteraemia. This study reports vanA gene cluster responsible for resistance in Pakistani population and frequently isolation of VRE from blood samples. Keywords: Enterococcus, Vancomycin resistant Enterococci, Pakistan","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77174928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Identification of the Potential Novel L-asparaginase producing Bacillus Strains from Soil 土壤中可能产生l -天冬酰胺酶的芽孢杆菌的分离与鉴定
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.2
Naushaba Nazli, Rukhsar Masood, Muhmmad Salman, B. Nasir, F. Shireen, A. Mehmood, F. Hussain, Shabir Ahmad, Sudhair Abbas Bangash
{"title":"Isolation and Identification of the Potential Novel L-asparaginase producing Bacillus Strains from Soil","authors":"Naushaba Nazli, Rukhsar Masood, Muhmmad Salman, B. Nasir, F. Shireen, A. Mehmood, F. Hussain, Shabir Ahmad, Sudhair Abbas Bangash","doi":"10.34091/ajls.4.1.2","DOIUrl":"https://doi.org/10.34091/ajls.4.1.2","url":null,"abstract":"L-Asparaginase is a well know enzyme for its antineoplastic potential and is widely used to treat acute lymphoblastic leukemia and lymphosarcoma. The present work describes the isolation and characterization of novel L-asparaginase producing Bacillus strains from soil. Soil samples were collected from three different locations such as fruit garden, dairy farm and agricultural land in Peshawar Khyber Pakhtunkhwa, Pakistan. The isolates were screened to produce L-asparaginase in growth medium supplemented with 1% L-asparagine using a phenol red indicator. Among 30 bacterial isolates, only two strains initially coded as A5 and FG7 showed L-asparaginase activity. Based on biochemical and 16S rRNA sequencing analysis, the isolate A5 and FG7 were identified as Bacillus amyloliquefaciens and Bacillus proteolyticus respectively. Different factors like pH and time were optimized for maximum L-asparaginase activity. Bacillus amyloliquefaciens showed maximum asparaginase activity at pH 7 after 24 hours incubation at 30oC, while Bacillus proteolyticus showed optimum activity at pH 7 after 48 hours of incubation at 30oC. The present study first time reported the production of L-asparginase enzyme from Bacillus amyloliquefaciens and Bacillus proteolyticus.\u0000\u0000Keywords: L-asparaginase, Bacillusamyloliquefaciens, Bacillus proteolyticus, 16sRNA.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"161 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73713447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Vegetation conditions in various plant communities and their performance in district Tharparkar, Sindh, Pakistan 巴基斯坦信德省Tharparkar地区不同植物群落的植被条件及其表现
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.16
Muhammad Waseem Kalroo, A. K. Pathan, M. S. Depar, I. A. Rajput, Behari Lal Meghwar, Arsalan Memon, Sarfaraz Ahmed, A. M. Lodhi, Arif Ali, M. Ibrahim
{"title":"Vegetation conditions in various plant communities and their performance in district Tharparkar, Sindh, Pakistan","authors":"Muhammad Waseem Kalroo, A. K. Pathan, M. S. Depar, I. A. Rajput, Behari Lal Meghwar, Arsalan Memon, Sarfaraz Ahmed, A. M. Lodhi, Arif Ali, M. Ibrahim","doi":"10.34091/ajls.4.1.16","DOIUrl":"https://doi.org/10.34091/ajls.4.1.16","url":null,"abstract":"Six villages were registered for the experimental purpose/plantation where more than 25,000 plants were planted to develop range forest. These plants were developed at Marvi nursery PARC-Arid Zone Research Institute, Umerkot and some were purchased from private nursery situated at Mirpurkhas. Results revealed that maximum plants mortality rate was found at village Ratnore and lowest mortality of plants was observed at Mithrio Charan. Plants damaged due to unknown reason (natural), termite attack and diseases. In various locations most of plants were affected by termites than others biotic and abiotic factors. The most influenced plant was Prosopis cinerera. Maximum plants survived numbers of grafted ber was found at village Ratnore, the biomass production of green and dry grasses was taken after harvesting. A highest green grass weight 21812.58 kg and dry 3439.83 kg were observed from elephant grass stock and lowest green weight 5001.92 kg from Gatten grass and dry weight 477.57 kg per acre were recorded from Rhode grass 1 (Sabri). Gatten grass and Rhode grass 1 (Sabri) were found lower weight. All these seven grasses showed excellent performance except Elephant grass and Gatten grass. These two grasses needed more water as compared to remaining five species. These grasses when fed by goats their milk production was increased and goats like to feed these grasses. The pictures of these grasses showed their appearance and health irrigated by underground brackish water with more than 2200 ppm.\u0000\u0000Keywords: grown, plants, performance, Tharparkar.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85301891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased pollution due to COVID-19 pandemic and bioremediation: A dire need of management COVID-19大流行造成的污染加剧和生物修复:迫切需要管理
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.7
Mala Khan
{"title":"Increased pollution due to COVID-19 pandemic and bioremediation: A dire need of management","authors":"Mala Khan","doi":"10.34091/ajls.4.1.7","DOIUrl":"https://doi.org/10.34091/ajls.4.1.7","url":null,"abstract":"The purpose of this study was to provide an overview of the effects of COVID-19 on macro-plastic pollution and to consider short-term and long-term scenarios for its possible environmental and human health consequences and to discuss possible strategies to address and overcome key challenges. It was emphasized that, whether or not they are involved in a healthy health crisis, future actions should reflect the balance between public health and environmental protection, as there is no doubt that they are interrelated with bioremediation and microbial metabolism in the presence of optimal environmental conditions. Bioremediation uses natural and psychological microorganisms to break down toxic and harmful substances aerobically and anaerobically. It can be handled by a mixed microbial consortium or pure microbial strains on site (in situ) or off site (in the case of case) Phyto therapy or even natural care. Nutrients suitable for impurities, especially petroleum hydrocarbons are researched technologies for us, for this a biotechnological approach designed for bioremediation and observation. COVID 19 Pandemic pushed the world into a new challenge of waste management. In general, physicochemical technologies are used, which allow for proper ecological biomedical processes. Some bacteria are mobile, feel the contamination, and move towards it to neutralize it. This review emphasizes on current pollution, which is result of COVID 19 pandemic, as people follow use and discard strategies for different things which is a threat to our environment.\u0000\u0000Keywords: Environment, waste management, COVID 19.","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85451469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thermal stable and proteinase-K resistant insecticidal toxins produced by Photorhabdus luminescens 光habdus luminesens产生的热稳定性和蛋白酶k抗性杀虫毒素
Volume 4 Issue 1 Pub Date : 2021-09-11 DOI: 10.34091/ajls.4.1.19
I. Ullah, M. A. Ghamdi
{"title":"Thermal stable and proteinase-K resistant insecticidal toxins produced by Photorhabdus luminescens","authors":"I. Ullah, M. A. Ghamdi","doi":"10.34091/ajls.4.1.19","DOIUrl":"https://doi.org/10.34091/ajls.4.1.19","url":null,"abstract":"Photorhabdus is lives in a mutualistic association with nematodes from the family Heterorhabditis. Bacteria of the Photorhabdus can survive independently and cause toxicity in a larger variety of insects. In the present study, insecticidal activity of non-portentous heat-stable metabolites of Photorhabdus luminescens was evaluated against Galleria mellonella. For this purpose, the culture extract of P. luminescens was injected into the G. mellonella larvae, which killed almost 90% of larvae within 48 h. The extract showed 100% insecticidal activity after heat treatment of 70 C for 30 min and even 60% and 40% activity lasted at 80 C and 90 C respectively. The extract also showed a high degree of thermal stability and was 100% actives after 60 min at 70 C. In addition, insecticidal activity was preserved up to 100% after all proteinase-K treatments (0 ?g/mL to 50 ?g/mL). The results revealed that the extracts were non-portentous and showed high thermal resistance and stability.\u0000\u0000Keywords: Photorhabdus, insecticidal activity, toxins, heat stable non-proteinaceous","PeriodicalId":23712,"journal":{"name":"Volume 4 Issue 1","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84086188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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