Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI最新文献

Vessel segmentation with deep learning (Conference Presentation) 基于深度学习的血管分割(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-13 DOI: 10.1117/12.2508038

Xiaojun Cheng, Sreekanth Kura, D. Boas

引用次数: 0

Label-free cellular viability imaging in 3D tissue spheroids with dynamic optical coherence tomography (Conference Presentation) 动态光学相干断层成像在三维组织球体中的无标记细胞活力成像(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-13 DOI: 10.1117/12.2508845

Ahbid Zein-Sabatto, Julia S. Lee, Madison K. Kuhn, Itzel Aponte, Bruno Felalaga, Jessica L. Sevetson, Blanche C. Ip, D. Hoffman-Kim, J. Morgan, Jonghwan Lee

引用次数: 0

3D phase imaging for thick biological samples (Conference Presentation) 厚生物样品的三维相位成像(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-13 DOI: 10.1117/12.2516567

Regina Eckert, Michael Chen, Li-Hao Yeh, L. Waller

{"title":"3D phase imaging for thick biological samples (Conference Presentation)","authors":"Regina Eckert, Michael Chen, Li-Hao Yeh, L. Waller","doi":"10.1117/12.2516567","DOIUrl":"https://doi.org/10.1117/12.2516567","url":null,"abstract":"Phase imaging provides quantitative structural data about biological samples as an alternative or complementary contrast method to the functional information given by fluorescence imaging. In certain cases, fluorescence imaging is undesirable because it may harm the development of living cells or add time and complexity to imaging pipelines. However, current 3D phase reconstruction methods, such as optical diffraction tomography [1], are often limited to a single-scattering approximation. This limits the amount of scattering that such 3D reconstruction algorithms can successfully handle, and therefore effectively limits the sample thickness that can be successfully reconstructed. More recent methods such as 3D Fourier ptychographic microscopy (FPM) have used intensity-only images combined with multiple-scattering models in order to reconstruct 3D volumes [2]. In practice, however, continuous biological samples on the order of 100 um thick are not well-reconstructed by 3D FPM, due to a lack of diverse information across the volume which creates an ill-posed inverse problem. To mitigate this, we introduce simultaneous detection coding in the form of pupil control to the 3D FPM capture scheme. Simple pupil coding schemes enabled us to capture diverse information across our volume. In concert with a beam propagation model that takes into account multiple scattering, this combination of illumination- and detection-side coding allows us to more stably reconstruct 3D phase for larger-scale biological samples.\u0000\u0000[1] E. Wolf, “Three-dimensional structure determination of semi-transparent objects from holographic data,” Opt. Commun. 1, 153–156 (1969). \u0000[2] L. Tian and L. Waller, “3D intensity and phase imaging from light field measurements in an LED array microscope,” Optica, 2, 104-111 (2015).","PeriodicalId":237104,"journal":{"name":"Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127776429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous detection of 3D orientation and 3D spatial localization of single emitters for nanoscale structural imaging (Conference Presentation) 纳米尺度结构成像中单发射器三维方向和三维空间定位的同时检测(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-13 DOI: 10.1117/12.2509891

S. Brasselet, V. Curcio, T. Brown, M. Alonso

{"title":"Simultaneous detection of 3D orientation and 3D spatial localization of single emitters for nanoscale structural imaging (Conference Presentation)","authors":"S. Brasselet, V. Curcio, T. Brown, M. Alonso","doi":"10.1117/12.2509891","DOIUrl":"https://doi.org/10.1117/12.2509891","url":null,"abstract":"Measuring single molecule 3D orientational behavior is a challenge that, if solved in addition to 3D localization, would provide key elements for super resolution structural imaging. Orientation contains indeed information on local conformational properties of proteins, while orientational fluctuations are signatures of local steric, charges or viscosity constraints. Both these properties are not perceptible in pure super resolution imaging, which relies on position localization measurements. Imaging 3D orientation together with 3D localization is however not easily accessible due to the intrinsic coupling between spatial deformation of the single molecules’ point spread function (PSF) and their off-plane orientations, as well as the requirement to measure six parameters which are not directly distinguishable (two angles of orientation, aperture of angular fluctuations, and three spatial position coordinates). In this work, we report a method that is capable of resolving these six parameters in a modality that is compatible with super resolution imaging. The method is based on the use of a stress-engineered spatially-variant birefringent phase plate placed in the Fourier plane of the microscope detection path. This modifies the PSF of single emitters in a way that can be non-ambiguously decomposed onto the nine 3D-analogs of the Stokes parameters. Moreover, the use of two complementary co/counter circular polarizations projections provides a non-ambiguous determination of the 3D spatial position of single emitters with tens of nanometers precision. This method, which opens to nanoscale structural imaging of proteins organization, is presented on model nano-beads emitters and applied to single fluorophores used for cytoskeleton labelling.","PeriodicalId":237104,"journal":{"name":"Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI","volume":"661 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116092664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D molecular orientation imaging by polarization IR microscopy (Conference Presentation) 偏振红外显微镜的三维分子定向成像(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-13 DOI: 10.1117/12.2510371

Y. J. Lee, J. Rowlette

引用次数: 0

Modeling cell-matrix interactions in ovarian cancer through image based 3D biomimetic scaffolds created by multiphoton excited fabrication (Conference Presentation) 通过多光子激发制造的基于图像的三维仿生支架来模拟卵巢癌细胞-基质相互作用(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-13 DOI: 10.1117/12.2510094

P. Campagnola, Samuel Alkmin, Rebecca Brodziski, Haleigh Simon, M. Patankar

引用次数: 0

Motion free micro-endoscopic system for imaging in freely behaving animals at variable focal depths using liquid crystal lenses (Conference Presentation) 使用液晶透镜在可变焦深下对自由行为动物进行成像的无运动微内窥镜系统(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-04 DOI: 10.1117/12.2508929

Arutyun Bagramyan, T. Galstian, A. Saghatelyan

引用次数: 1

An open source software tool for arbitrary vector beams in free-space and stratified media (Conference Presentation) 自由空间和分层介质中任意矢量光束的开源软件工具(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-04 DOI: 10.1117/12.2507413

P. Munro

引用次数: 0

Visualization of three-way and higher order data sets (Conference Presentation) 三向和高阶数据集的可视化(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-04 DOI: 10.1117/12.2516224

B. Wise

引用次数: 0

Automated large-volume confocal imaging system (Conference Presentation) 自动大体积共聚焦成像系统(会议报告)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI Pub Date : 2019-03-04 DOI: 10.1117/12.2509270

Yen-Yin Lin, An-lun Chin, Chia-Jund Lee, Wei-Kun Chang, Chien-Chung Fu, A. Chiang, Y. Y. Lin

引用次数: 0