Vietnam Journal of Biotechnology最新文献_第9页

Identification of the causative mutation in the ITGB2 gene in a LAD1 patient by whole exome sequencing 通过全外显子组测序鉴定LAD1患者ITGB2基因的致病突变

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/15886

Nguyễn Thị Kim Liên, Nguyen Thi Van Anh, Nguyen Van Tung, Duong Anh Linh, Nguyen Thi Phương Mai, Nguyen Huy Hoang

{"title":"Identification of the causative mutation in the ITGB2 gene in a LAD1 patient by whole exome sequencing","authors":"Nguyễn Thị Kim Liên, Nguyen Thi Van Anh, Nguyen Van Tung, Duong Anh Linh, Nguyen Thi Phương Mai, Nguyen Huy Hoang","doi":"10.15625/1811-4989/15886","DOIUrl":"https://doi.org/10.15625/1811-4989/15886","url":null,"abstract":"Leukocyte adhesion deficiency type 1 (LAD1) is a rare congenital immunodeficiency disease. The cause of disease is determined to be the mutations in the ITGB2 gene that codes for CD18, the beta chain of beta-2 integrins, leads to decreased expression or functioning of CD18. This deficiency leads to severe impairment of leukocyte adhesion to the vascular wall and leukocyte migration to sites of infection and inflammation. LAD1 has also been associated with inhibition of interleukin-23 and interleukin-17 resulting in a hyperinflammatory and chronic inflammation. Patients with LAD1 typically present in early infancy with recurrent, life threatening infections that are frequently fatal before 2 years of age without hematopoietic stem cell transplant (HSCT). However, LAD1 is difficult to diagnose and many LAD1 patients die at a young age despite intensive antibiotic therapy. Accurate diagnosis requires detailed clinical information (delayed umbilical cord loss, severe periodontitis, delayed wound healing and sores, skin abscesses, and recurrent infection), and confirmation the absence of integrins by flow cytometric analysis. A better understanding of the molecular characteristics of this disease is needed to raise awareness and definitive diagnosis infants with LAD1. To definitive diagnosis, whole exome sequencing and Sanger sequencing were performed in an eighteen-month-old boy with severe leukocytosis, recurrent infections, delayed wound healing, and hepatosplenomegaly associated with an acquired cytomegalovirus infection. Two variants: One previously reported mutation (c.533C>T, p.Pro178Leu) and one novel variant (c.59-1G>A), in the ITGB2 gene were detected. These results can be used for definitive genetic diagnosis, genetic counseling, as well as a prenatal diagnosis in LAD1 patients.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85131460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A study on biological activities of Bouea macrophylla Griff leaf extract 大叶菊叶提取物的生物活性研究

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/16059

P. Hai Ha, Tran Kien Cuong, Nguyen Thi Thu Nha, Nguyen Phan Hoang Lan, N. H. Thuan Anh, Bach Long Giang, Pham Minh Quan, Tran Quoc Toan, Pham Quoc Long, Nguyen Thanh Luan

{"title":"A study on biological activities of Bouea macrophylla Griff leaf extract","authors":"P. Hai Ha, Tran Kien Cuong, Nguyen Thi Thu Nha, Nguyen Phan Hoang Lan, N. H. Thuan Anh, Bach Long Giang, Pham Minh Quan, Tran Quoc Toan, Pham Quoc Long, Nguyen Thanh Luan","doi":"10.15625/1811-4989/16059","DOIUrl":"https://doi.org/10.15625/1811-4989/16059","url":null,"abstract":"Bouea macrophylla in the family Anacardiaceae is a prominent fruit in Southeast Asia, especially in Vietnam with several prospects. This study focused on the bioactive components and biological effects of B. macrophylla ethanol leaf extract. Phytochemical analysis revealed various compounds including polyphenol, tannin, saponin, reducing agent, flavonoid, and essential oil in the extract. The DPPH assay showed the remarkable antioxidant capacity of the extract (IC50 = 6.4 ± 0.08 μg/ml), which is stronger than ascorbic acid (IC50 = 9.35 ± 0.12 μg/ml). The disc diffusion method and minimal inhibitory concentration test displayed the significant antibacterial ability of the extract against Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, and Bacillus cereus at the range from 10 - 15 mg/ml, but no activity against Staphylococcus aureus. Moreover, the MTT assay was performed the noticeable anticancer activity of the extract in HepG2 (IC50 = 72.33 ± 0.68 μg/ml), and MCF-7 cells (IC50 = 50.25 ± 1.36 μg/ml). Taken together, this study indicated the effects of B. macrophylla ethanol leaf extract from Vinh Long Province, Vietnam on antibacterial, antioxidant, and anticancer properties. Therefore, B. macrophylla possibly is used in the food industry and medicine.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87188706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico study of cytochrome P450 alleles and phenotypic distribution in Vietnamese population 越南人群细胞色素P450等位基因的基因分析及表型分布

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/16265

Pham Ngoc Ha, Nguyen Anh Tuan, Trinh Thi Xuan, Truong Nam Hai, Tran Dang Hung, N. Cuong

{"title":"In silico study of cytochrome P450 alleles and phenotypic distribution in Vietnamese population","authors":"Pham Ngoc Ha, Nguyen Anh Tuan, Trinh Thi Xuan, Truong Nam Hai, Tran Dang Hung, N. Cuong","doi":"10.15625/1811-4989/16265","DOIUrl":"https://doi.org/10.15625/1811-4989/16265","url":null,"abstract":"Cytochrome P450 enzymes play an important role in phase I drug metabolism, accounting for approximately 75% of the enzymatic processes. We investigated the allele and phenotypic distributions of five important CYP genes (CYP2B6, CYP3A5, CYP2C9, CYP2C19, CYP2D6) in the Vietnamese population by using Stargazer and the PharmCAT tool to call star alleles and translating them into phenotypes based on the available dataset of PharmGKB. We compared our computational analysis of the Vietnamese distributions with those of East Asia, Europe, America and other super populations, as well as with previous experimental research. The allele frequencies and phenotypic distributions of the five important CYP genes in the Vietnamese population are similar to those in East Asia while significantly different from other populations. In silico analysis also provided consistent results with previous experimental studies. In addition, the resultant data from our research contributes to the database of genetic variations in pharmacogenetics and constructs the fundamentals for future basic and applied research. ","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"199 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85480913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and characterization of recombinant nattokinase from Bacillus subtilis R0H1 枯草芽孢杆菌R0H1重组纳豆激酶的纯化及特性研究

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/16027

Nguyen Thi Thuy Ngan, Le Tuan, N. Huong

{"title":"Purification and characterization of recombinant nattokinase from Bacillus subtilis R0H1","authors":"Nguyen Thi Thuy Ngan, Le Tuan, N. Huong","doi":"10.15625/1811-4989/16027","DOIUrl":"https://doi.org/10.15625/1811-4989/16027","url":null,"abstract":"Nattokinase (NK) is a fibrinolytic enzyme with the potential for fighting cardiovascular diseases (CVD) thanks to its antithrombotic, antihypertensive, anticoagulant, anti-atherosclerotic, and neuroprotective effects. Nattokinase was first discovered and purified from soybean fermented food by Bacillus subtilis natto. To enhance NK’s activity and simplify downstream processes, production of recombinant NK using several microbial expression systems such as Escherichia coli, B. subtilis, and Lactococcus lactic has been studied. Among all of them, B. subtilis is a prominent host for overproduction of functional proteins which can be secreted directly into the culture medium. In this study, recombinant NK from B. subtilis R0H1 was purified using two-step membrane filtration. Results showed 3.2-fold increase in activity and a recovery rate of more than 80%. Molecular weight of NK was approximately 28 kDa and its fibrinolytic degradation capacity was proved according to SDS-PAGE. The optimal pH and temperature of this NK were 8.5 and 55°C, respectively. The enzyme activity was boosted by Mg2+, Ca2+ and obviously inhibited by Co2+, Zn+2, Fe2+, and SDS. The apparent Km and Vmax with fibrin as the substrate were 3.08 mM and 6.7 nmol/min, respectively. The results suggested that membrane filtration is a useful method for purification of recombinant NK from B. subtilis R0H1. Therefore, application of membrane system is proposed to purify NK at the pilot scale. In addition, our findings indicated that recombinant NK produced in B. subtilis R0H1 showed high and stable proteolytic activity in slightly alkaline pH and at high temperature. It also exhibited strong fibrinolytic activity again both substrates: fibrinogen and fibrin.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85781985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Citrus limon peel extraction, determination of main chemical components and effectiveness in repellency against Aedes mosquito dengue fever vector 柑橘柠檬皮提取工艺优化、主要化学成分及对登革热媒介伊蚊的驱避效果测定

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/16365

Phung Thi Kim Hue, Tran Van Loc, Le Tri Vien, Le Dung Sy, Ho Viet Hieu, L. Nhung, Le Thanh Do, Pham Thi Minh Khoa

{"title":"Optimization of Citrus limon peel extraction, determination of main chemical components and effectiveness in repellency against Aedes mosquito dengue fever vector","authors":"Phung Thi Kim Hue, Tran Van Loc, Le Tri Vien, Le Dung Sy, Ho Viet Hieu, L. Nhung, Le Thanh Do, Pham Thi Minh Khoa","doi":"10.15625/1811-4989/16365","DOIUrl":"https://doi.org/10.15625/1811-4989/16365","url":null,"abstract":"Currently, scientific publications are focusing on the pharmacological actions of Citrus limon's extract and essential oil. Its essential oil is rich in bioactive monoterpenoids, such as D-limonene, β-pinene, γ-terpinene. Citrus limon essential oils have been recognized as the potential source of natural insecticides because of their selectivity, ability to be biologically degraded into non-toxic compounds, low impacts on biodiversity and the environment. Many previous studies have reported anti-bacterial, anti-fungal, anti-inflammatory, anti-cancer, hepato-regenerative, and cardio-protective activities of Citrus limon essential oil. In this study, we collected the discarded Citrus limon peel source in Gia Lai province to utilize as a material to build an optimized extraction process with the following criteria: extraction solvent, solvent/sample ratio, extraction temperature, and extraction time. The results showed a stable extraction process with a maximum extraction efficiency of 4.02%, at 40°C, for 3 hours, with two extraction times using 95% ethanol for solvent. Using GC/MS method, the determined limonene content accounted for 12.2% of the extract. The Citrus limon peel extract exhibited potency against Aedes aegypti (arbovirus vector) at a concentration of 0.01 mL, with protection time of 70 minutes and biting percentage of 0.9%, compared to negative control with statistically significant (P < 0.05). The above results correspond with the most recent publications about the effects of mosquito repellence of certain plant-based essential oils. This study has proven that Citrus limon peel in this locality signifies a promising candidature for future studies regarding its main active compound, limonene, in the control of dengue-transmitting vectors. Therefore, Citrus limon peel extract brings hope to develop new mosquito repellency products in the future.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72970858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of sequence polymorphism of OsHKT1;5 gene in rice (Oryza sativa L.) 水稻OsHKT1;5基因序列多态性分析

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/15969

Nguyen Huy Duong, Tran Xuan An, Do Thi Phuc

{"title":"Analysis of sequence polymorphism of OsHKT1;5 gene in rice (Oryza sativa L.)","authors":"Nguyen Huy Duong, Tran Xuan An, Do Thi Phuc","doi":"10.15625/1811-4989/15969","DOIUrl":"https://doi.org/10.15625/1811-4989/15969","url":null,"abstract":"In salt stress conditions, the excessive accumulation of Na+ ion in the cytoplasm could cause ion toxicity, metabolic imbalances, and reduction in growth of plant. The ability of maintaining a low Na+ concentrations or low Na+/K+ ratio in cytosol plays the important roles of plant adapt to salinity stress. In rice, the HKT transporter family has been proven to be critical for salt tolerance and the maintenance of Na+, K+ homeostasis under salinity conditions. In this study, we conducted the sequence polymorphisms analysis in the OsHKT1;5 coding region in order to detect the potential allelic variants in some local rice cultivars in Viet Nam. The exon 1 region and exon 2-3 region of OsHKT1;5 gene were amplified by PCR in two separate reactions. After direct sequencing of PCR products, the full length coding region of OsHKT1;5 gene was obtained and compared to reference Nipponbare OsHKT1;5 coding sequence. Accordingly, twelve nucleotide substitutions in coding sequence of OsHKT1;5 were identified, including six non-synonymous substitutions (G382A, C418G, G551A, C994G, C1183G, A1630C) and the other six synonymous substitutions (G225A, C1038G, G1152A, G1261A, G1304C, C1608T). Further analysis revealed that six non-synonymous substitutions (G382A, C418G, G551A, C994G, C1183G, A1630C) caused the changes in amino acids (D128N, P140A, R184H, H332D, V395L, and S544R). The single nucleotide polymorphism A1630C leading substitution of S544R was newly found in the Chiem Rong cultivar. In silico analysis of protein structure and post-translational modifications indicated that amino acid substitutions had no influence on protein structure but led to changes in post-translational modifications such as phosphorylation and SUMOylation.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76801513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first record of Metacordyceps neogunnii (Metacordyceps, Clavicipitaceae) isolated from larva of Lepidoptera in Vietnam: morphological, phylogenetic characterization and chemical constituent analysis 越南鳞翅目新奥古尼元虫草(Metacordyceps neogunnii)的首次分离记录:形态、系统发育特征及化学成分分析

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/16033

L. D. Thuan, L. Thuy, Le Cong Hac, Nguyễn Hoàng Mai, N. Giảng, Truong Binh Nguyen

{"title":"The first record of Metacordyceps neogunnii (Metacordyceps, Clavicipitaceae) isolated from larva of Lepidoptera in Vietnam: morphological, phylogenetic characterization and chemical constituent analysis","authors":"L. D. Thuan, L. Thuy, Le Cong Hac, Nguyễn Hoàng Mai, N. Giảng, Truong Binh Nguyen","doi":"10.15625/1811-4989/16033","DOIUrl":"https://doi.org/10.15625/1811-4989/16033","url":null,"abstract":"An entomopathogenic fungus, specimen DL0091 parasitized on the larvae of Lepidoptera, was collected from Lang Biang Biosphere Reserve, located in Lam Dong Province, Vietnam. The specimen DL0091 has been analyzed to be contained numerous chemical constituents, especially containing adenosine of 634 mg/Kg and cordycepin of 35.2 mg/Kg. Due to containing many bioactive compounds, DL0091 was promised to be a precious natural source that could be applied in fields of medicine and function food for health care. For classification, based on the morphology analysis, it was identified as Metacordyceps neogunnii (Metacordyceps, Clavicipitaceae) sharing the similar characteristics of M. neogunnii T.C. Wen & K.D. Hyde. Morphology of this species differed from Cordyceps neogunnii (Berk.) Berk., by many characteristics, such as the larger stroma of DL0091 (15–130 mm x 2–6 mm), of asci (550–680 μm × 5–8 μm), etc. Additionally, the combined multi-gene phylogenetic analysis, including ITS, Tef and Rpb1, well supported its systematic position in the clade of M. neogunnii, which was used as traditional herb in China and other Asian countries. In summary, DL0091 was identified as M. neogunnii, containing many bioactive compounds, could be used as the medicinal potential in human healthcare.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77700636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and molecular identification of obligate thermophiles from hot springs in Ba Ria – Vung Tau and Khanh Hoa provinces, Vietnam 越南巴里亚省和庆化省温泉中专性嗜热菌的分离和分子鉴定

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/15863

Tran Kha Mong, Le Thi Thanh Van, Nguyen Vu Phong, Nguyen Huu Tri

{"title":"Isolation and molecular identification of obligate thermophiles from hot springs in Ba Ria – Vung Tau and Khanh Hoa provinces, Vietnam","authors":"Tran Kha Mong, Le Thi Thanh Van, Nguyen Vu Phong, Nguyen Huu Tri","doi":"10.15625/1811-4989/15863","DOIUrl":"https://doi.org/10.15625/1811-4989/15863","url":null,"abstract":"Environments with temperatures from 50°C to 80°C are rare in nature and are almost exclusively associated with geothermal regions including hot springs, solar-heated soils and volcanic areas. Thermophilic bacteria already exist and prefer in such habitats. Since innate tolerance to thermal environment and potential chassis for extracellular enzymes such as lipase, protease and amylase, which are utilized widely in the industrial fermentation, thermophilic bacteria have been becoming one of the objects for microbiologists worldwide, recently. This study aimed to isolate and identify thermophilic bacteria from hot springs in several provinces in Vietnam such as Ba Ria - Vung Tau and Khanh Hoa. In the results, six moderate thermophilic bacterial strains (namely BM7, BS5, NS1, NS3, NS4, and NW6) that could grow at 55oC were purified from the hot spring ecosystems. All micro morphology of isolates were recorded as rod-shaped, Gram positive, and endospore forming. The results of 16S rDNA sequencing and phylogenetic analysis showed that these isolate belonged to group I of Bacillus genus (the thermophilic group). The isolated strains NS1, NS3, NS4, BS5, NW6 and BM7 were identified to belong to the Bacillus genus, species as Bacillus sp. Resulting strains are potential candidates for industrial applications due to its stable fitness in a hash environment, particularly at high temperature. In addition, this study provides a useful insight into the diverse community of thermophilic bacteria (Bacillus spp.) in several hot springs of Vietnam, that can be applied as bacterial cell factories to produce biomaterials, biofuels, or valuable compounds in the future.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90231603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into Paphiopedilum x Dalatense Aver. (Orchidaceae) origin based on morphological and molecular markers 对罂粟属植物的认识。基于形态学和分子标记的兰科植物起源

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/15909

Tran Thai Vinh, H’ Yon Nie Bing, Vu Kim Cong, D. Tham, Ngo Phuong Linh, Le Ngoc Trieu, Nông Văn Duy

{"title":"Insight into Paphiopedilum x Dalatense Aver. (Orchidaceae) origin based on morphological and molecular markers","authors":"Tran Thai Vinh, H’ Yon Nie Bing, Vu Kim Cong, D. Tham, Ngo Phuong Linh, Le Ngoc Trieu, Nông Văn Duy","doi":"10.15625/1811-4989/15909","DOIUrl":"https://doi.org/10.15625/1811-4989/15909","url":null,"abstract":"Paphiopedilum callosum, Paphiopedilum villosum and Paphiopedilum ´ dalatense are endangered species and are currently listed as Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES, 2019) Appendix I species. Thus, the international trade for these naturally occurring species is forbidden. However, the in vitro propagated materials and their inter-species hybrids forced by breeders can be traded for commercial purposes. P. ´ dalatense is an endemic slipper orchid species of Vietnam with high aesthetic value. Currently, the natural reserves of this species have been greatly reduced by overexploitation. In this study, P. ´ dalatense was demonstrated to be the reversible hybrid species between P. callosum and P. villosum based on both morphological and molecular markers. P. ´ dalatense possesses intermediate morphological characteristics between P. callosum and P. villosum which could be recorded in leaf, in flower and its components such as lip, petal, and dorsal sepal. There were 23 sites in ITS1-5.8S rRNA-ITS2 region sequence of P. ´ dalatense which possess the nucleotide characteristic of both P. callosum and P. villosum. There were two out of three P. ´ dalatense investigated samples possessed partial matK gene which was identical to the corresponding sequence in P. villosum and the last sample possessed identical partial matK gene to P. callosum. Proving P. ´ dalatense to be the reversible hybrid species between P. callosum and P. villosum is the essentially scientific base for commercial breeding of P. ´ dalatense by artificial hybridization which contributes to avoid overexploiting the natural P. ´ dalatense resources.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90234530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total phenolic, flavonoid contents and antioxidant activity of tamarind seed and pulp extracts 罗望子籽和果肉提取物的总酚、类黄酮含量及抗氧化活性

Vietnam Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.15625/1811-4989/15930

Le Phuong Ha, Nguyen Van Ngoc, Nguyen Thi Trang Huyen, Le Thi Thu Hang, Nguyen Thi Kieu Oanh, Tran Mai Thi Tuyet, Nguyen Thi Mai Phuong, N. M. Minh Hong

{"title":"Total phenolic, flavonoid contents and antioxidant activity of tamarind seed and pulp extracts","authors":"Le Phuong Ha, Nguyen Van Ngoc, Nguyen Thi Trang Huyen, Le Thi Thu Hang, Nguyen Thi Kieu Oanh, Tran Mai Thi Tuyet, Nguyen Thi Mai Phuong, N. M. Minh Hong","doi":"10.15625/1811-4989/15930","DOIUrl":"https://doi.org/10.15625/1811-4989/15930","url":null,"abstract":"Tamarind (Tamarindus indica) has long been known for its high nutrition content and pharmacological potential. However, there is lack of studies on the content of antioxidants, phenolic and flavonoid contents of tamarind seed grown in Vietnam. Thus, the aim of this study was to compare the seeds and pulps of Tamarindus indica from three different areas across Vietnam including Son La, Hai Phong and Sai Gon with regard to the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of their water and methanol extracts, as well as their cytotoxicity on a normal BKH-21cells. TPC and TFC were evaluated by the Folin–Ciocalteu reagent and aluminum chloride, respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays were used to investigate antioxidant capacity. The safety of T. indica extracts was assessed by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Our results showed that the methanolic extracts yielded higher TPC (742.919 ± 50.360 mg GAE/g extract), TFC (68.492 ± 0.023 mg QE/g extract) and possessed stronger free radical scavenging activity (IC50 of 52.5 µg/mL) compared to that of water extracts. T. indica seeds from all three regions possessed higher TPC, TFC and antioxidant activity than those of pulps. Regarding the safety, in vitro analysis showed that tamarind seed and pulp extracts only became toxic to BHK-21 cell line at a very high concentration with IC50 values range from 143.77 µg/mL to 620.35 µg/mL. This study revealed that T. indica seeds and pulps can serve as functional food as well as potential antioxidants in pharmaceutical products.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88503812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1