Veterinarski Arhiv最新文献_第2页

The effects of pre- and postnatal exposure to perfluorooctanoic acid on the expression of major reproduction-related genes in the mouse hypothalamus and gonads 产前和产后全氟辛酸暴露对小鼠下丘脑和性腺主要生殖相关基因表达的影响

4区农林科学

Veterinarski Arhiv Pub Date : 2023-09-10 DOI: 10.24099/vet.arhiv.1766

Hun Kim, Neelesh Sharma, Yun-ho Bae, Sung-Jin Lee

{"title":"The effects of pre- and postnatal exposure to perfluorooctanoic acid on the expression of major reproduction-related genes in the mouse hypothalamus and gonads","authors":"Hun Kim, Neelesh Sharma, Yun-ho Bae, Sung-Jin Lee","doi":"10.24099/vet.arhiv.1766","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1766","url":null,"abstract":"Perfluorooctanoic acid (PFOA), a ubiquitous environmental pollutant reported to be an endocrine disruptor, is used in many industrial and consumer products. Although the adverse effects of PFOA on the reproductive health of animals and humans have been widely reported, most studies have focused on assessing the anatomical features and conventional histology of adult gonads. Therefore, the molecular mechanisms activated in the hypothalamus and gonads following PFOA exposure during the pre- and postnatal periods are not clear. This study used a mouse model to evaluate the effects of PFOA exposure on the alteration of molecular mechanisms in the hypothalamus and gonads during the prenatal and postpartum periods. Changes in gene and protein expression following PFOA exposure were evaluated by quantitative polymerase chain reaction and Western blotting, respectively. Kisspeptin 1 and gonadotropin-releasing hormone expression in the hypothalamus of female and male mouse pups was significantly decreased. Additionally, Cyp17a1 expression was upregulated in male offspring testes, while Cyp17a1 and Cyp19a1 expression was downregulated in female offspring ovaries. Changes at the molecular level due to PFOA exposure in the early stages of development did not show sex-related differences in the hypothalamus; however, such differences were confirmed in the gonads. These results could be used as basic data to study the molecular mechanisms underlying the reproductive dysfunction caused by PFOA exposure in the early stages of embryonic development.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of herbal supplements on the carcass characteristics, fatty acid profile and meat quality attributes of broilers 草药添加物对肉鸡胴体特性、脂肪酸分布和肉质性状的影响

4区农林科学

Veterinarski Arhiv Pub Date : 2023-09-10 DOI: 10.24099/vet.arhiv.1759

Gudapureddy Vijaya Bhaskar Reddy, Pidugu Amaravathi, Bhaskar Ganguly, Arub Ratan Sen, Bandaru Venkata Vivekananda Reddy

{"title":"The effect of herbal supplements on the carcass characteristics, fatty acid profile and meat quality attributes of broilers","authors":"Gudapureddy Vijaya Bhaskar Reddy, Pidugu Amaravathi, Bhaskar Ganguly, Arub Ratan Sen, Bandaru Venkata Vivekananda Reddy","doi":"10.24099/vet.arhiv.1759","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1759","url":null,"abstract":"The present investigation was carried out to determine the effect of two herbal formulations, AV/LMP/10 (T1) (garlic, Allium sativum) and AV/HLP/16 (T2) (mixture of gugal, Commiphora mukul and fenugreek, Trigonella foenum-graecum with 50:50%), on the carcass characteristics, proximate composition, lipid profile including fatty acids, and meat quality attributes in Vencobb 400 broiler chickens. Broilers fed with the AV/HLP/16 (T2) formulation had significantly (P<0.05) superior carcass characteristics in terms of pre-slaughter weight, head, neck, shank, skin, stomach, intestine, giblet, and wholesale cut yield than the control and broilers supplemented with AV/LMP/10 (T1). Meat obtained from the broilers fed with the T2 formulation had a significantly (P<0.01) higher dressing percentage and lean percentage than the control broilers and the T1 supplemented broilers. Meat obtained from the broilers fed with the T2 formulation had significantly (P<0.01) higher moisture and protein content, and lower fat content than the control birds. Meat obtained from the broilers fed with the T2 formulations had significantly (P<0.01) lower triglycerides and higher phospholipids and cholesterol content than meat obtained from the control birds. A significant (P<0.01) increase was observed in the myristic, palmitic, oleic, palmitoleic, linoleic and behenic acid percentages, and a decrease in the stearic acid percentage in meat obtained from the broilers fed with the T2 formulation. Addition of the T2 formulation significantly (P<0.01) improved the physico-chemical quality and sensory scores of the chicken meat. The results of this study revealed that addition of herbal formulations had a significant effect on the carcass characteristics, proximate composition, fatty acid profile and meat quality attributes in comparison with the control birds.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prevalence, antibiotic resistance and biofilm formation ability of Enterococcus spp. isolated from food products in Algeria 阿尔及利亚食品分离肠球菌流行率、抗生素耐药性和生物膜形成能力

4区农林科学

Veterinarski Arhiv Pub Date : 2023-09-10 DOI: 10.24099/vet.arhiv.1755

Yacine Titouche, Madjid Akkou, Fawzi Rostane Meklati, Amel Kaced, Mohand Teffane, Abdelmadjid Tahi, Karim Houali

{"title":"The prevalence, antibiotic resistance and biofilm formation ability of Enterococcus spp. isolated from food products in Algeria","authors":"Yacine Titouche, Madjid Akkou, Fawzi Rostane Meklati, Amel Kaced, Mohand Teffane, Abdelmadjid Tahi, Karim Houali","doi":"10.24099/vet.arhiv.1755","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1755","url":null,"abstract":"Enterococci are ubiquitous bacteria in the normal intestinal microbiota of both humans and animals. They can be used in the food industry as starter or probiotic cultures. However, some species have emerged as an important nosocomial pathogens, and have been implicated in severe multi-resistant infections. In this study, a total of 235 food products were analyzed for the presence of Enterococcus spp. Overall, 54 (22.9%) out of the 235 samples were contaminated and 54 strains were isolated. The latter were identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and tested for antibiotic susceptibility against seven antibiotic molecules, in addition to their ability to form a biofilm. The predominant species was Enterococcus faecalis (70.4%), followed by Enterococcus hirae (12.9%), Enterococcus faecium (11.1%) and Enterococcus durans (5.5%). The highest resistance profile was ascribed to tetracycline (66.7%) and penicillin G (33.3%). Six enterococcal isolates (11.1%) were resistant to at least three antibiotic families. All isolated strains were able to form a biofilm and exhibited gelatinase activity. However, only 4 (7.4%) were β-hemolytic. This study revealed that food products might play a role in the spread of enterococci through the food chain to humans with these virulence and resistance characteristics. As a result, continuous investigations are necessary to assess the health hazards associated with the consumption of contaminated food products.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence of bovine viral diarrhoea in organized herds in India 印度有组织牛群中牛病毒性腹泻的血清流行率

4区农林科学

Veterinarski Arhiv Pub Date : 2023-09-10 DOI: 10.24099/vet.arhiv.1560

Laxmi Narayan Sarangi, Kota Sri Naga Leela Surendra, Samir Kumar Rana, Naveena Thodangala, Amitesh Prasad, Ponnanna Nadikerianda Muthappa, Girish Kumar Sharma

{"title":"Seroprevalence of bovine viral diarrhoea in organized herds in India","authors":"Laxmi Narayan Sarangi, Kota Sri Naga Leela Surendra, Samir Kumar Rana, Naveena Thodangala, Amitesh Prasad, Ponnanna Nadikerianda Muthappa, Girish Kumar Sharma","doi":"10.24099/vet.arhiv.1560","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1560","url":null,"abstract":"Bovine viral diarrhoea (BVD) is an important infectious viral disease affecting cattle populations all over the world. In addition to direct loss caused by the disease, the virus causes immunosuppression thereby predisposing the host to other diseases. A cross-sectional study was undertaken to detect the prevalence of BVD in 14 well-organized herds located in different parts of India. A total of 880 serum samples (646 cattle and 234 buffaloes) were screened by a commercial ELISA kit, detecting antibodies towards the p80 (NS3) region of BVDV. The overall true prevalence was 56.67% (95% CI: 53.26-60.02%) and within herds, it ranged from 0-99.99%. The prevalence rate was higher in cattle (65.42%) than in buffaloes (32.49%) and the difference was statistically significant. Further, a significant difference in prevalence among cattle breed types was recorded, with the lowest in indigenous cattle (16.49%) followed by crossbreeds (16,97% and exotic breeds (87.80%). Higher positivity was detected among females (68.87%) than males (48.83%) but this difference was not significant, as revealed by multivariate regression analysis. Of the 10 semen stations studied, the prevalence varied from 9.72% to 72.68%. However, none of the animals from these semen stations turned positive in the antigen ELISA test, suggesting the antibodies detected in this study were from past infections. On the two dairy farms/bull mother farms showing very high positivity, two (one each) persistently infected cows were detected during whole herd screening by antigen ELISA test. One bull mother farm was free of BVD antibodies suggesting it is possible to maintain BVDV-free herds. The present study indicates the endemicity of BVDV in Indian organized herds, and therefore a suitable testing strategy and management should be adopted in response to testing to control the introduction and further transmission of the disease on farms.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statins modify the response of chicks to challenges with xylazine-ketamine and carbaryl 他汀类药物改变了雏鸡对氯胺酮和西威因的反应

4区农林科学

Veterinarski Arhiv Pub Date : 2023-09-10 DOI: 10.24099/vet.arhiv.1769

Hussein M. Rashid, Fouad K. Mohammad

{"title":"Statins modify the response of chicks to challenges with xylazine-ketamine and carbaryl","authors":"Hussein M. Rashid, Fouad K. Mohammad","doi":"10.24099/vet.arhiv.1769","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1769","url":null,"abstract":"Statins are known to affect brain function in a manner not related to their dyslipidemic effects. The purpose of the present study was to assess the behavioral response of a chick model (7-14 days old) after single treatments with the statins atorvastatin, fluvastatin or simvastatin at 100 mg/kg, orally, to a pharmacological challenge with an anesthetic regimen of xylazine (5 mg/kg)-ketamine (20 mg/kg), intramuscularly, and a toxicological challenge with the reversible cholinesterase inhibiting insecticide carbaryl (250 mg/kg, orally). Only simvastatin significantly reduced the duration of xylazine-ketamine anesthesia by 47%. Carbaryl at 250 mg/kg, orally induced signs of cholinergic poisoning (57.1 - 100%) in chicks within 3.14 min, and 57.1% death occurred within 85 min. Toxicological challenge of statin-treated chicks with carbaryl also induced signs of cholinergic poisoning, but with varying percentages of reductions compared to the control (carbaryl) group. The reductions in 4 h carbaryl-induced lethality in chicks pre-treated with atorvastatin, fluvastatin and simvastatin were 43, 57 and 29%, respectively, below that of the control value. Correspondingly, their toxicity scores decreased by 18, 18 and 11%, respectively. These data suggest that statins might modulate the functional status of the brain in a manner that affects the impact of centrally acting drugs or toxicants, and hence the behavioral outcomes in chicks. Further studies are warranted on the behavioral effects of statins after prolonged therapy.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0