Veterinarski Arhiv最新文献

{"title":"Evaluation of hepatorenal damage induced by benzylpenicilloic acid in mice","authors":"Ruixue Hu, Jian Guo, Ke Zhao, Honglin Ren, Zengshan Liu, Shiying Lu, Yansong Li, Yang Wang, Pan Hu","doi":"10.24099/vet.arhiv.2618","DOIUrl":"https://doi.org/10.24099/vet.arhiv.2618","url":null,"abstract":"Benzylpenicilloic acid (BPNLA) is a byproduct of the natural degradation and enzymatic hydrolysis of penicillin. BPNLA primarily enters and accumulates in the human body through the consumption of animal products. Previous research has mainly focused on drug resistance and the resulting allergic reactions, but as the accumulation of the drug increases, toxicity also manifests. The liver and kidneys are the main organs for drug metabolism and excre- tion of many drugs, and are most prone to toxic effects. Therefore, this study aimed to investigate whether BPNLA causes hepatorenal toxicity. All C57BL/6 mice were randomly assigned to four groups and administered orally 2.925, 146.25, and 7312.5 μg/kg b.wt (body weight) of BPNLA, or an equivalent volume of 0.9% saline (control) for 35 days. The results showed that BPNLA could lead to a decrease in the organ coefficient of the liver, as well as struc- tural abnormalities in the liver and kidneys. Further research found that liver and kidney function markers, lipid peroxidation markers (MDA), and proinflammatory cytokines (TNF-α and IL-1β) significantly increased compared to the control group. Moreover, the levels of antioxidant markers (GSH, SOD, GPX) decreased in a dose-dependent manner. In summary, the results clearly demonstrated that even relatively low concentrations of BPNLA can cause liver and kidney damage, highlighting the need for concern regarding human exposure to BPNLA.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"95 3","pages":"339-350"},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.24099/vet.arhiv.2618","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kisspeptin-10 modulates hormone secretion and proliferation of follicular granulosa cells by upregulating expressions of the kiss-1r gene","authors":"Suocheng Wei, Enyu Gao, Linglong Xu, Mengyuan Pei, Zhaofang Yuan","doi":"10.24099/vet.arhiv.2580","DOIUrl":"https://doi.org/10.24099/vet.arhiv.2580","url":null,"abstract":"The present study aimed to determine the subcellular localization of KISS-1 protein, and also to investigate the effects of Kisspeptin-10 (KP-10) on the proliferation viability and hormone secretion of follicular granulosa cells (FGCs), and its molecular mechanisms. FGCs were cultured in vitro in DMEM media supplied with the different doses of KP-10 (0,10,100 and 1000 nmol/L) which were allocated into a control group (CG), experiment group 1 (EP1), experiment group 2 (EP-2) and experiment group 3 (EP-3), respectively, CG, EP-1, EP-2 and EP-3. Subcellular localization of FSHR and KISS-1 proteins in FGCs were assayed using immunofluorescence assay. A Cell Counting Kit 8 (CCK8), real time quantitative PCR (qPCR), and western blotting were used to detect the proliferation activities and expression levels of genes and proteins in the FGCs. Elisa kits were applied to measure the contents of estrogen (E2) and progesterone (P4). Immunofluorescence assay showed that KISS-1 protein was positively expressed in sheep FGCs and mainly distributed in the cytoplasm. At 48h and 72h following KP-10 treatment, the cell viability of the FGCs in groups EP-2 and EP-3 were significantly enhanced compared to CG (P<0.05 or P<0.01). The estrogen (E2) and progesterone (P4) contents of group EP-2 were increased in comparison with CG (P<0.01). Additionally, expression levels of Kiss-1R, StAR, 3β-HSD and CYP11 mRNAs in group EP-2 were remarkably increased compared to CG (P<0.05 or P<0.01) with increments of 65.9%, 30.7%, 73.4% and 71.4%, respectively. In conclusion, KISS-1 proteins were located mainly in the cytoplasm of FGCs. KP-10 could enhance FGC vitality, and promote progesterone and estrogen secretion, which is possibly achieved by upregulation of expression levels of Kiss-1R, StAR, 3-β-HSD and CYP11 genes. KP-10 significantly upregulated mRNA and protein levels of Bcl-2 and PI3K (P<0.01), and downregulated expressions of mRNA and protein levels of Bcl-2 and PI3K (P<0.05). Our outcomes provide a solid basis for searching after novel improvements of reproduction of animals.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"95 1","pages":"23-38"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cleavage of chicken vimentin by the reticuloendotheliosis virus protease","authors":"Tong Zhu, Minxun Song, Bing Huang, Yufeng Li, Feng Hu","doi":"10.24099/vet.arhiv.2416","DOIUrl":"https://doi.org/10.24099/vet.arhiv.2416","url":null,"abstract":"The protease (PR) encoded by reticuloendotheliosis virus (REV) is deeply involved in the lifecycle and infection process of a virus in that it cleaves the viral polyproteins into their mature structural proteins and replication enzymes. Besides this essential role in the lifecycle of a virus, the enzymatic activity of the REV PR targeting chicken vimentin remains unknown. In this study, the recombinant chicken vimentin, fused with a GST-tag, was expressed in the Escherichia coli system as a soluble form. The soluble vimentin was purified using Glutathione Sepharose 4B. Then, the in vitro cleavage of chicken vimentin by the PR of REV was analyzed. The results showed that chicken vimentin was cleaved by the REV PR. Furthermore, Nano LC-MS/MS analysis showed that the REV PR cleaved chicken vimentin between leucine-239 and glutamine-240, alanine-261 and alanine-262, and histidine-431 and serine-432, respectively. Moreover, the cleavage sites identified in this study were different from other known naturally occurring cleavage sites of other retroviruses. For the first time, we showed that the REV PR cleaved the chicken vimentin at specific sites in chicken vimentin. These results support the possibility that chicken vimentin may serve as a substrate within REV-infected cells, facilitating our understanding of avian retroviruses.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"94 4","pages":"305-312"},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.24099/vet.arhiv.2416","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of taurochenodeoxycholic acid on the glucocorticoid receptormediated PLC-IP3 -calcium pathway in synoviocytes derived from a rat adjuvant arthritis model","authors":"Jindi Wu, Fan Bai, Sangjide Guanqia, Peifeng Li, Jinshan Cao, Yinghong Qian, Wei Mao","doi":"10.24099/vet.arhiv.2081","DOIUrl":"https://doi.org/10.24099/vet.arhiv.2081","url":null,"abstract":"Taurochenodeoxycholic acid is one of the main active components of the bile acid pool and has important anti-inflammatory and immunomodulatory properties. This study aimed to explore the therapeutic effects and mechanism of action of taurochenodeoxycholic acid in inflammatory arthritis using a rat adjuvant arthritis model. Fibroblast-like synoviocytes were derived from rats with adjuvant arthritis and cultured using the adherent tissue explant method. Western blotting, enzyme-linked immunosorbent assay, and fluorescent probe-based methods were used to detect the effects of taurochenodeoxycholic acid on glucocorticoid receptor expression, phospholipase C protein levels, and inositol trisphosphate and intracellular free Ca2+ concentrations in primary rat fibroblast-like synoviocytes. Taurochenodeoxycholic acid significantly induced the expression of glucocorticoid receptor (P<0.05) and phospholipase C (P<0.05) and enhanced phospholipase C phosphorylation. Moreover, taurochenodeoxycholic acid significantly increased the levels of inositol trisphosphate (P<0.05) and intracellular free Ca2+ concentrations. Our results suggest that taurochenodeoxycholic acid activates the phospholipase C-inositol trisphosphate-Ca2+ signaling pathway by increasing glucocorticoid receptor expression. These findings provide a theoretical foundation for future studies on the molecular mechanisms underlying taurochenodeoxycholic acid-based treatment of adjuvant arthritis. In conclusion, taurochenodeoxycholic acid exerted anti-inflammatory and immunomodulatory effects by enhancing glucocorticoid receptor expression via non-genomic signalling.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"94 1","pages":"77-86"},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.24099/vet.arhiv.2081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Moringa oleifera meal supplementation on the antioxidant status of crossbred heifer calves","authors":"Asif N. Sherasiya, Pravin M. Lunagariya, Jigar H. Patel, Mohsinkhan M. Pathan, Mahesh M. Chaudhary, Malhar R. Khant","doi":"10.24099/vet.arhiv.1751","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1751","url":null,"abstract":"The experiment was conducted to evaluate the anti-oxidant effect of Moringa oleifera meal-supplementation in the total mixed ration, replacing the compounded concentrate mixture for crossbred heifer calves. A total of twenty-one crossbred heifer calves were selected and grouped into three treatments based on body weight and age. The leaves (84%) and soft twigs (16%) of Moringa oleifera were mixed to prepare the Moringa oleifera meal. Compounded concentrate mixture, ground nut straw, hybrid napier grass, mineral mixture and common salt were used at 50, 23, 25, 1 and 1%, respectively to formulate the control total mixed ration (T1). The compounded concentrate mixture was replaced with the Moringa oleifera meal at 5.0 and 7.5 % to formulate total mixed rations T2 and T3. The whole blood was collected from each calves in the morning before feeding and watering at the beginning (0 day), middle (at 60 day) and end (at 126 day) of the experiment. The total leukocyte counts, total erythrocyte counts, haematocrit and platelet count of crossbred heifer calves differed non-significantly (P>0.05) between the treatment groups, and were within normal physiological range. The serum albumin, glucose, creatinine, urea, alkaline phosphatase, alanine amino transaminase, calcium and phosphorus differed non-significantly between the treatment groups. However, feeding with Moringa oleifera resulted in higher (P0.05) higher after feeding with the 5.0 and 7.5% Moringa oleifera meals. Feeding with 5.0 and 7.5% Moringa oleifera meal in the total mixed ration, replacing the high protein compounded concentrate mixture, to growing crossbred heifer calves had no adverse effect on haematological and biochemical constituents, and resulted in a non-significant (P>0.05) higher antioxidant capacity in the crossbred heifer calves.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of SYBR Green I-based quantitative PCR assay for identification of porcine circovirus 1, 2 and 3","authors":"Chang Liu, Yao Tian, Li-mei Zheng, Ying-Ru Ma, Jian-Li Shi, Chen Li, Shao-Jian Xu, Xiao-Yan Wu, Hong Han, Jun Li, Xian-Jie Han","doi":"10.24099/vet.arhiv.2123","DOIUrl":"https://doi.org/10.24099/vet.arhiv.2123","url":null,"abstract":"Porcine Circovirus (PCV) includes Porcine Circovirus 1(PCV1), Porcine Circovirus 2 (PCV2) and Porcine Circovirus 3 (PCV3). In recent years, co-infection exists between PCV1, PCV2 and PCV3 serotypes. Therefore, it is particularly necessary to establish a fast, specific and sensitive SYBR Green I real-time quantitative PCR detection method for PCV1, PCV2 and PCV3. In this experiment, specific primers were selected and the reaction conditions were optimized. A real-time quantitative PCR identification method was established. The results showed the detection limits of this assay were 40.3 copies/μl for PCV1, 25.2 copies/μl for PCV2 and22.4 copies/ μl for PCV3. There was no cross-reactivity with swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine pseudorabies virus (PRV) and porcine parvovirus (PPV). The intra-assay and inter-assay coefficients of variation were less than 1%. The test results of 100 PCV suspected positive samples revealed that the PCV1, PCV2 and PCV3 singular infection rate was 10% (10/100), 64% (64/100) and 52% (52/100), respectively. The PCV1 and PCV2 co-infection rate was 8% (8/100), the PCV1 and PCV3 co-infection rate was 7% (7/100), the PCV2 and PCV3 co-infection rate was 26% (26/100), and the PCV1, PCV2 and PCV3 co-infection rate was 7% (7/100). This method has good specificity, sensitivity and stability. It provides a promising tool for rapid differential detection of PCV1, PCV2 and PCV3.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of lidocaine and its combination with ketamine for distal intravenous regional anesthesia (DIVRA) in bovines","authors":"Vipin Kumar Yadav, Anil Kumar Gangwar, Sangeeta Devi Khangembam, Yogendra Yadav, Ravi Prakash Goyal","doi":"10.24099/vet.arhiv.1753","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1753","url":null,"abstract":"The hoof diseases of cattle can be managed surgically under intravenous regional anesthesia (IVRA). For routine induction of IVRA, a tourniquet is placed circumferentially at the metacarpus/metatarsus. In the present study, hoof diseases of cattle were corrected using a modified IVRA technique. The cattle with hoof ailments were randomly divided into two groups and a tourniquet was placed just distal to the dew claws instead of at the metacarpus/metatarsus in order to decrease the dose of anesthetic. In group I lidocaine (2mg/kg) and in group II a mixture of lidocaine and ketamine (2mg/kg+1.5mg/kg) was injected into the axial digital vein to induce distal intravenous regional anesthesia (DIVRA). The heart rate, respiration rate, systolic and diastolic pressure were unaffected in both groups. Oxygen saturation was significantly (P<0.05) lower between 5 and 60 minutes in group I and between 15and 40 minutes in group II animals. The sensory and motor block onset time was shorter, and the sensory and motor block recovery time was longer in group II animals as compared to group I animals. It was concluded that the DIVRA technique using lidocaine alone and lidocaine admixed with ketamine are suitable for hoof examination and surgery.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"2016 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphism of the growth hormone (GH) gene and its association with growth traits in the Kilakarsal breed of sheep","authors":"Seevagan Muniasamy, Jeichitra Veerasamy, Rajendran Ramanujam, Krishnaswamy Gopalan Tirumurugaan","doi":"10.24099/vet.arhiv.1452","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1452","url":null,"abstract":"The goal of this study was to investigate the single nucleotide polymorphism of the growth hormone gene and its association with growth traits in the Kilakarsal breed of sheep. Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP) of the growth hormone gene (422 bp) with HaeIII restriction enzyme revealed an A781G transition mutation at the exon 2 and the existence of two genotypes, viz., AA (366 bp and 56 bp) and AB (422 bp, 366 bp and 56 bp). The occurrence of the B allele is due to the A781G transition in the exon 2 of the growth hormone gene, which causes an amino acid change from Serine to Glycine. The genotype BB was absent in the Kilakarsal sheep population. The absence of the BB genotype at the A781G locus of the growth hormone gene in the Kilakarsal sheep population indicates that the natural selection process might be acting against this particular genotype through reduced viability or early embryonic death. Further, the statistical analysis revealed the significantly higher yearling weight of the AB than the AA genotype (P=0.038), and an appreciable difference of 1.40 kg in 9-month weight. The highly significant (P<0.001) Chi-square value (29.94) showed that the population is not the under Hardy-Weinberg equilibrium. This indicates that molecular markers associated with body weight should be explored for their use in marker assisted selection in Kilakarsal population.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of dietary white button mushrooms (Agaricus bisporus) on the kinetics of changes in the proportion of peripheral blood CD4+ CD8+ T lymphocytes in lambs","authors":"Branimira Špoljarić, Ana Shek Vugrovečki, Damir Mihelić, Ivona Žura Žaja, Silvijo Vince, Daniel Špoljarić, Mario Živković, Marijana Mirjana Kardum Paro, Ksenija Vlahović, Marko Samardžija, Nada Vijtiuk, Maja Popović","doi":"10.24099/vet.arhiv.1797","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1797","url":null,"abstract":"The primary focus of the current study was to determine the potential benefits of supplementing sheep diet with white button mushrooms (WBM) in terms of growth, health and the kinetics of systemic CD4+ CD8+ memory T lymphocytes in lambs. Forty-five female lambs (Lika breed) were divided into three groups: A – the control group fed on a free-range pasture for the 222 days of the experiment, while groups B and C were housed in a separate facility for 42 days and fed either a commercial feed mixture (FM) or a FM supplemented with 15% of freshly prepared WBM, respectively, and ad libitum forage. For the remaining 180 days of the experiment, both groups (B and C) of lambs were kept free-range and fed pasture only. The lambs were monitored daily starting on Day 0 (or 90 days of age) before the treatments, weighed and blood sampled on Days 0, 21, 42 and 222, and were clinically observed for the incidence/severity of diarrhea and/or other signs of disease. In addition to morbidity, mortality was also monitored, and dead lambs were examined for gross pathology changes. The lambs fed FM supplemented with WBM (group C) had significantly higher body weight gain (P<0.05) on Days 42 and 222. They were neither diarrheic nor had any mortality cases throughout the experiment. Also, these lambs had a significantly increased (P<0.05) proportion of CD4+CD8+ T cells on Days 42 and 222. The data obtained supported our assumption of the efficacy of dietary WBM in the immunostimulation of CD4+CD8+ memory T lymphocytes in lambs, resulting in protection against on-farm diarrhea and providing an increased growth rate.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computed tomographic finding of bilateral optic nerve head drusen in a dog – a case report","authors":"Chandrasekaran Niranjana, Mohamed Shafiuzama, Ramani Chandrasekara Iyer","doi":"10.24099/vet.arhiv.1774","DOIUrl":"https://doi.org/10.24099/vet.arhiv.1774","url":null,"abstract":"Optic nerve head drusen are acellular calcified deposits in the optic nerve head reported in humans. A six year old, male, Rottweiler dog with a history of respiratory distress and epistaxis for the previous two months was anaesthetised and a computed tomographic scan of the head was performed. The axial view of the computed tomography scan with a slice thickness of 1mm was viewed in a soft tissue window (380x40 HU). There was an incidental discovery of a characteristic discrete round hyper dense area in the optic nerve head region in both eyes during the routine computed tomographic scan. The Hounsfield Unit of the optic nerve head drusen in the right eye measured 91 HU, of 2 mm2 and in the left eye 131 HU of 1 mm2 confirming the diagnosis of optic nerve head drusen. To the authors’ knowledge, this is the first report of optic nerve head drusen in a dog.","PeriodicalId":23547,"journal":{"name":"Veterinarski Arhiv","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136108518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}