Voprosy meditsinskoi khimii最新文献_第7页

[Realities and hopes of gene therapy]. [基因治疗的现实与希望]。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

R I Zdanov, N V Semenova, A I Archakov

引用次数: 0

[Target delivery of functional genes in gene therapy using carbohydrate containing vectors]. [使用含碳水化合物载体的基因治疗中功能基因的靶向递送]。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

G G Krivtsov, R I Zhdanov

引用次数: 0

[Gene therapy of chronic infections of the urogenital system using cytotoxic peptides]. [细胞毒性肽对泌尿生殖系统慢性感染的基因治疗]。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

V N Lazarev, V M Govorun, N M Aleksandrova, Iu M Lopukhin

引用次数: 0

[Retroviral vectors in gene therapy]. 基因治疗中的逆转录病毒载体。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

V S Prasolov, D S Ivanov

引用次数: 0

[Gene therapy of monogenic hereditary diseases. Duchenne myodystrophy]. 单基因遗传性疾病的基因治疗。杜乡myodystrophy]。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

V S Baranov, A N Baranov

引用次数: 0

[Prospects for gene therapy of cardiovascular diseases]. 【心血管疾病基因治疗展望】。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

E V Parfenova, V A Tkachuk

引用次数: 0

[Nonviral gene transfer in vivo in gene therapy]. [基因治疗中的体内非病毒基因转移]。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

E V Bogdanenko, Iu V Sviridov, A A Moskovtsev, R I Zhdanov

引用次数: 0

[Gene therapy of hereditary diseases]. [遗传疾病的基因治疗]。

Voprosy meditsinskoi khimii Pub Date : 2000-05-01

E K Ginter

{"title":"[Gene therapy of hereditary diseases].","authors":"E K Ginter","doi":"","DOIUrl":"","url":null,"abstract":"In the review the main advantages in development of the approaches to gene therapy of hereditary diseases are presented. Now more than 1000 genes of hereditary diseases are mapped and some hundreds are cloned which is prerequisite for gene therapy. The transfer of the recombinant gene into the cell and the subsequent expression of the transgene product are the rate-limiting steps for successful gene therapy. A variety of methods, including the use of physical methods, modified viruses and synthetic vectors, are currently being used in experiments and clinical trials. Since the approval and initiation of the first human gene therapy trial to treat ADA deficiency, there have been several dozen approved gene therapy trials but clear clinical result was stated for ADA deficiency only. Cystic Fibrosis, CF was among several hereditary diseases which were considered as a target for gene therapy. Experiments on development of recombinant gene constructions, gene delivery by adenovirus vectors and liposomes as well as by other constructions into epithelial lung cells, gene expression and on the safety of gene therapy procedures were relatively successful. Phase 1 gene therapy clinical trials of CF showed that some unaccounted physiological peculiarities of lung tissue of the patients diminished effectiveness of gene transfer, longevity of CFTR gene expression and in some cases unexpected immunological complications arises during clinical trials. Now an intensive attempt to overcome these problems in gene therapy of CF are undertaken.","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"46 3","pages":"265-78"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21864052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of thyroxine on the activity of some enzymes of energy metabolism in bone marrow myeloid cells and blood neutrophils from piglets]. [甲状腺素对仔猪骨髓细胞和血液中性粒细胞能量代谢酶活性的影响]。

Voprosy meditsinskoi khimii Pub Date : 2000-03-01

N O Babich, G L Antoniak, M F Tymochko

引用次数: 0

[Bilirubin as an endogenous intermediary in the activation of CYP1A1 expression upon exposure to ultrasound]. [胆红素在超声作用下激活CYP1A1表达中的内源性中介作用]。

Voprosy meditsinskoi khimii Pub Date : 2000-03-01

A Iu Grishanova, T V Zueva

{"title":"[Bilirubin as an endogenous intermediary in the activation of CYP1A1 expression upon exposure to ultrasound].","authors":"A Iu Grishanova, T V Zueva","doi":"","DOIUrl":"","url":null,"abstract":"The induction of cytochrome P4501A1 (CYP1A1) enzyme activity reflects the increased ligand-dependent transcriptional activity of the cognate CYP1A1 gene. The list of ligands includes various xenobotics such as polycyclic and halogenated aromatic hydrocarbons. Until recently, similar role for endogenous compounds was unknown. In the present study the ability of the endogenous heme metabolite, bilirubin, to regulate CYP1A1 activity was examined. The following parameters were investigated: expression of CYP1A1 in rat liver at a level mRNA, protein and functional activity under at the experimental rising of blood bilirubin level. The influence of local ultrasound contact treatment of rats hepatic area in vivo (the intensity 0.4 W/cm2 and duration time of 10 minutes) on blood unconjugated bilirubin concentration and parameters of CYP1A1 transcriptional activity was also investigated. The ultrasound contact action on rat hepatic are increased blood unconjugated bilirubin concentration. The rise of bilirubin levels of in rat blood after intravenous administration of bilirubin as well as after ultrasound treatment was accompanied by increased mRNA CYP1A1, protein and functional activity of CYP1A1. The comparison of these data with that of time-dependent changes of parameters of CYP1A1 transcriptional activity under ultrasound action and experimental rising of blood bilirubin level suggest that induces CYP1A1 and may be an intermediate in the activation of CYP1A1 expression under ultrasound action.","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"46 2","pages":"117-26"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21727697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0