VNU Journal of Science: Natural Sciences and Technology最新文献_第6页

Structure and Bonding in the Complex of Zinc with 4,4’-Diacetylcurcumin 锌与4,4′-二乙酰姜黄素配合物的结构与键合

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2023-02-16 DOI: 10.25073/2588-1140/vnunst.5351

Nguyen Huu Tho

引用次数: 0

Cloning and Expression of 3 Chymotrypsin-like Protease of SARS-CoV-2 in E. coli using pET28a Vector 利用pET28a载体克隆和表达SARS-CoV-2 3个凝乳胰蛋白酶样蛋白酶

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2023-02-16 DOI: 10.25073/2588-1140/vnunst.5509

Vu Thi Duy Ly, T. T. Giang, Ngo Thu Trang, Dinh Nho Thai, Phan Tuấn Nghĩa, Nguyen Thi Hong Loan

{"title":"Cloning and Expression of 3 Chymotrypsin-like Protease of SARS-CoV-2 in E. coli using pET28a Vector","authors":"Vu Thi Duy Ly, T. T. Giang, Ngo Thu Trang, Dinh Nho Thai, Phan Tuấn Nghĩa, Nguyen Thi Hong Loan","doi":"10.25073/2588-1140/vnunst.5509","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5509","url":null,"abstract":"The 3 chymotrypsin-like protease (3CLpro) of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is one of the primary targets for the development of antiviral drug therapies as it plays a critical role in viral replication. In this study, the gene encoding for SARS-CoV-2 3CLpro (918 bp) was amplified from the cDNA of the virus by polymerase chain reaction (PCR) and cloned into the pGEM-T vector. 3CLpro was then inserted into the expression vector pET28a at the end of the 6 histidine residue encoding sequence to form a fusion protein (6xHis-3CLpro). The 6xHis-3Clpro construct was successfully expressed in E. coli. The expression of 3CLpro was highest when E. coli BL21(DE3) RIL harboring pET28a-3CLpro vector was cultured in LB medium at 20 oC, induced by 1.0 mM Isopropyl thiogalactopyranosie (IPTG) when cell density measured by optical density at 600 nm (OD600) reached 0.7-0.8 and harvested after 24 hours of induction. The recombinant 3CLpro was purified by Ni-sepharose affinity chromatography under denaturation conditions. The purified 3CLpro showed to have a 41 kDa band on sodium dodesyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using polyclonal anti-3CLpro antibody and hydrolyzed a fluorescent specific substrate of 3CLpro after renaturation.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":"1959 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91249639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Creation of Standard DNA for Detection and Quantification of White Spot Syndrome Virus in Shrimps by Real-time PCR (qPCR) 实时荧光定量PCR (qPCR)检测和定量对虾白斑综合征病毒标准DNA的建立

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-19 DOI: 10.25073/2588-1140/vnunst.5508

T. Cuong, Phan Tuấn Nghĩa, Nguyen Thi Hong Loan

引用次数: 0

Decolorization of Dye Wastewater by Activated Carbons Produced from Peanut Shell using ZnCl2 Impregnation Method 花生壳活性炭氯化锌浸渍法对染料废水的脱色研究

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-19 DOI: 10.25073/2588-1140/vnunst.5228

N. Quan, Nguyen Minh Hanh, L. Oanh

引用次数: 0

Analysis of Triacylglyceride and Conjugated Fatty Acid Compositions in Seed Oils of some Plants of Cucurbitaceae Family in Vietnam 越南部分葫芦科植物种子油中甘油三酯和共轭脂肪酸组成分析

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-16 DOI: 10.25073/2588-1140/vnunst.5361

N. Anh, V. Anh

{"title":"Analysis of Triacylglyceride and Conjugated Fatty Acid Compositions in Seed Oils of some Plants of Cucurbitaceae Family in Vietnam","authors":"N. Anh, V. Anh","doi":"10.25073/2588-1140/vnunst.5361","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5361","url":null,"abstract":"Conjugated fatty acids were known as potential sources for the pharmaceutical industry with beneficial bioactivities, including anti-cancer effects, anti-diabetes, anti-obesity, and anti-inflammatory properties. In the present work, characteristics of triglycerides and fatty acids of the seed oils of five plants (Momordica Cochinchinensis (Lour.) Spreng, MomordicaMomordica charantia, Thladiantha nudiflora Hemsl, and Trichosanthes cucumerina var. anguina) in Vietnam were analyzed by UV and FTIR spectroscopy, and reversed phase high performance liquid chromatography. Triacylglycerides compositions of the seed oils were determined using RP-HPLC with DAD detector connected with MS detector in series. The seed oils of M. Cochinchinensis, M. subangulata, and M. charantia are rich sources of conjugated octadecatrienoic acid with α-eleostearic acid (C18:39E,11E,13Z) as the base acid and β-eleostearic as the minor conjugated acid. In the seed of Thladiantha nudiflora (both α-eleostearic and punic acid (C18:39Z,11E,13Z) were synthesized with approximately equal proportions. In T. cucumerina var. anguina seed oil, punicic acid was synthesized as the main conjugated fatty acid. In addition, some oil parameters, such as oil yield and refractive index of these seed oils, were determined.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84698508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lithocarpus pacoensis (Fagaceae) a new species from Hoa Binh Province, Vietnam 文章标题越南和平省石栎属(壳斗科)一新种

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-08 DOI: 10.25073/2588-1140/vnunst.5459

Ngoc Nguyen Van, S. T. Hoang, Binh Thi Hoang

引用次数: 1

Effects of Led Light Intensity and Carbon Dioxide Concentration on the Growth of Spinach (Spinacia oleracea L.) in a Plant Factory 植物工厂中Led光强和二氧化碳浓度对菠菜生长的影响

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-08 DOI: 10.25073/2588-1140/vnunst.5511

Phạm Châu Thùy, Nguyen Le Khanh, Nguyen Nhu Duy

{"title":"Effects of Led Light Intensity and Carbon Dioxide Concentration on the Growth of Spinach (Spinacia oleracea L.) in a Plant Factory","authors":"Phạm Châu Thùy, Nguyen Le Khanh, Nguyen Nhu Duy","doi":"10.25073/2588-1140/vnunst.5511","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5511","url":null,"abstract":"This research aimed to investigate the effects of light intensity and the carbon dioxide (CO2) concentration on spinach’s growth during vegetative stage in plant factory (PF). Two experiments were conducted with different CO2 concentration in closed chamber (500 ppm and 800 ppm). In each experiment, plants were hydroponically grown under three light intensities of 115, 140 and 160 µmol.m-2s-1. Growth’s parameters were determined at different growth stages (T30, T37 and T44 respectively to 30, 37 and 44 days after sowing). ANOVA analysis and non-parametric Kruskal analysis of variance were used to evaluate the statistical significance of the treatment effects. The results showed that, before 30 days old, the increase of light intensity was not significantly effected to the growth of plant. During later growing periods (30 days old afterward), the increase of light intensity remarkably boost up yield’s related traits such as leaf number, fresh mass and dry mass under 500ppm CO2 concentration. The combination of light intensity and CO2concentration at 800ppm showed the plant growth indicators increased significantly at the T37 and T44 stages (after 30 days old), especially at the last week of growth period, fresh weight and dry weight of plants were significantly different at the light treatment L2, increased 37% and 57.6%, respectively as compared to light treament only. The results suggested that, during latest growing period, the average light intensity of 140 µmol.m-2s-1combining with elevating CO2 concentration is an alternatively way to enhance spinach growth. The findings of this study could be helpful for growers to improve growing conditions for a better development of spinach in the indoor farming (PF)","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76468001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Constituents of Caesalpinia bonduc 山葵属植物的化学成分

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-08 DOI: 10.25073/2588-1140/vnunst.5500

Le Thi Huyen, Nguyen Thi Thu Hau

引用次数: 0

Synthesis of Green Chitosan – Silver Nanoparticle Composite Material: Parameter Optimization and Physicochemical Characterization 绿色壳聚糖-纳米银复合材料的合成:参数优化及理化表征

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-03 DOI: 10.25073/2588-1140/vnunst.5363

T. Y, D. Thien, T. Cong, Tran Thi Nu, Lai Thi Bich Thuy, Nguyễn Thị Thúc, Le Quang Tuan, Nguyen Dang Dat, K. Hileuskaya, J. Kalatskaja, V. Kulikouskaya

{"title":"Synthesis of Green Chitosan – Silver Nanoparticle Composite Material: Parameter Optimization and Physicochemical Characterization","authors":"T. Y, D. Thien, T. Cong, Tran Thi Nu, Lai Thi Bich Thuy, Nguyễn Thị Thúc, Le Quang Tuan, Nguyen Dang Dat, K. Hileuskaya, J. Kalatskaja, V. Kulikouskaya","doi":"10.25073/2588-1140/vnunst.5363","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5363","url":null,"abstract":"In this study, response surface methodology (RSM) was carried out with four parameters: chitosan concentration, AgNO3 concentration, ascorbic acid concentration and time with the objective to obtain minimum mean particle size of silver nanoparticles (response Y). The chitosan-silver nanoparticle composite material (CTS-AgNPs nanocomposite) obtained experimentally from the optimal conditions was characterized by evaluating some of their physical-chemical properties using combined technique including visible ultraviolet spectroscopy (UV -Vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), infrared spectra (IR) and X-ray diffraction. The optimal conditions were chitosan concentration of 19.2 %, silver nitrate of 26 mM, ascorbic concentration of 33 mM and time of 4.25 hours. Spherical Ag particles obtained experimentally were well dispersed in chitosan matrix with the average diameter of around 2÷8 nm with a narrow size distribution and homogeneous dispersion","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79590995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of DNA Markers from Vietnamese Asarum L. Species 越南细辛属植物DNA标记分析

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-12-01 DOI: 10.25073/2588-1140/vnunst.5499

H. Hue, L. Kỳ, N. Hoang

{"title":"Analysis of DNA Markers from Vietnamese Asarum L. Species","authors":"H. Hue, L. Kỳ, N. Hoang","doi":"10.25073/2588-1140/vnunst.5499","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5499","url":null,"abstract":"The Asarum L. genus includes 128 species with a lot of medicinal effects and has been used extensively in the folk prescriptions for treatment of several diseases such as bronchitis, asthma, rheumatism, and hepatitis. In the world, many researches showed that the Asarum L. species have many kinds of the essential oils such as myristicin, methyl eugenol, myrcene, asafrol, borneol, safrol or α-pinene and substances as secquiterpenes, sterols, naringenin or glycosyl flavonoids. Some reports in Vietnam have been conducted on analyzing secondary compounds of these species. However, it has done a little research on classification of these species through molecular markers. Indeed, the DNA barcoding will help overcome difficulties in recognizing many plants which were endangered species or it is rare materials with high value in medicine. In the study, four DNA barcoding regions - ITS1, ITS2, matK and rpoC were analyzed from some Asarum species that are three species of A. splendens, A. yentuense and A. petelotii. The four barcodes were well amplified by PCR and sequenced by Sanger principal. After that, analyzing sequences showed ITS1, ITS2 had the highest polymorphism, in addition, the markers and matK marker were absolutely suitable for distinguishing the Asarum L. species. The above results are of great significance for the application of the barcodes in the identification of Asarum species, in order to correctly use the medicinal plant sources.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84696425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2