Ukrainian Biochemical Journal最新文献

{"title":"The concentration of ceruloplasmin in blood of tumor-bearing rats after administration of a dirhenium(III) compound and cisplatin","authors":"O. Hrabovska, K. L. Shamelashvili, O. Shtemenko, N. Shtemenko","doi":"10.15407/ubj91.06.079","DOIUrl":"https://doi.org/10.15407/ubj91.06.079","url":null,"abstract":"Contemporary investigations of mechanisms of resistance to platinides showed the key role of copper metabolism in cancer patients and proposed possible methods to attenuate the resistance by modulation of copper transporting mechanisms. In this vein, investigation of ceruloplasmin (Cp) levels – the main coppercontaining protein in blood, in experiments with tumor-containing animals upon cytostatics administration is topical and has great importance. the concentration of Cp was measured in the serum of tumor-bearing rats with ordinary (t8) and resistant to cisplatin (t8*) Guerin’s carcinoma upon administration of cisplatin and quadruple bonding dirhenium(III) compound dichlorotetra-μ-isobutyratodirhenium(ІІІ) (I) in different medicamental forms. It was shown that development of tumor in t8 group led to increasing of concentration of Cp in 3.7 times and in T8* group – more than in 8 times in comparison to control, confirming the essential role of Cp in the formation of resistance phenomenon. administration of cisplatin together with I led to effective inhibition of tumor in groups with t8 and t8*, indicating decreased resistance in the group t8*. Greater reduction of Cp levels was observed in the groups with t8* upon administration of the rhenium-plati num antitumor system, than in groups with t8, that underlines the importance of further investigations of the dirhenium(III) compounds in the resistance to cytostatics cancer models. Some mechanisms concerning the regulation of copper homeostasis and properties of nano-composites are discussed.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"79-85"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45571384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen peroxide as a signal mediator at inducing heat resistance in wheat seedlings by putrescine","authors":"Yu. E. Kolupaev, A. I. Kokorev, T. Yastreb, E. I. Horielova","doi":"10.15407/ubj91.06.103","DOIUrl":"https://doi.org/10.15407/ubj91.06.103","url":null,"abstract":"Polyamines are multifunctional stress metabolites of plants. However, information on the effect of exo­ genous polyamines on plant resistance to high temperatures is contradictory, and it remains unclear which signal mediators are involved in the realization of their physiological effects. The possible involvement of hydrogen peroxide as a mediator under the action of exogenous diamine putrescine on the resistance of etiolated wheat seedlings (Triticum aestivum l.) to hyperthermia (10-minute heating at 46 °C) and the functioning of antioxidant system was investigated. It was established that the treatment of seedlings with putrescine in 0.25–2.5 mM concentrations caused a significant increase in their heat resistance. In response to the putrescine effect, a transient increase in the H2o2 content occurred in the root cells. This effect was eliminated by treatment of seedlings with a diamine oxidase inhibitor aminoguanidine and an NADPH oxidase inhibitor imidazole. These inhibitors, as well as the scavenger of hydrogen peroxide dimethylthiourea (DMTU), mitigated the effects of increased heat resistance of seedlings and increased activity of superoxide dismutase and catalase caused by putrescine. Under the influence of DMTU and imidazole, but not aminoguanidine, the effect of increasing the activity of guaiacol peroxidase in the roots of seedlings treated with putrescine was eliminated. The conclusion was made about the role of hydrogen peroxide and the possible participation of diamine oxidase and NADPH oxidase in its formation during the implementation of the stress­protective effect of putrescine on wheat seedlings.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"91 1","pages":"103-111"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41363462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypertriglyceridemia is associated with long-term risk of cardiovascular events and specific comorbidity in very high-risk hypertensive patients","authors":"Ya Danylo, Halytskyi Lviv, Korolyuk O Ya","doi":"10.15407/ubj92.02.008","DOIUrl":"https://doi.org/10.15407/ubj92.02.008","url":null,"abstract":"Although hypertriglyceridemia (HTG) frequently occurs in hypertensive patients and may increase cardiovascular risk, the need and way of its reduction remain controversial. The objectives of the research was to compare lipid profiles, parameters of glucose homeostasis, comorbidity, a 5-year survival without cardiovascular events in very high risk hypertensive (VHRH) patients with and without HTG, who received moderate intensity atorvastatin therapy. After initial assessment 107 VHRH subjects were divided into two groups, i.e., without (n=49) and with HTG (n=58). During observation once annually patients were interviewing about prior hospitalizations with further screening for diabetes. Combined endpoint included hospitalization due acute myocardial infarction, decompensated heart failure, stroke or death. Survival was analyzed by Kaplan-Meier’s method. Nonparametric methods were used for statistical analysis. Higher median values of logarithmic value of triglycerides-to-HDL-cholesterol ratio, lipid accumulation product, fasting insulin, and HOMA index were observed in group 2 ( P< 0.002) that reflect predominance of small dense LDL particles, ectopic lipid deposition and insulin resistance. Patients with HTG more commonly had type 2 diabetes (58.6% vs 34.5%, including first-detected cases during initial assessments and observation, P=0.02), liver steatosis (81.0% vs 55.1%, P=0.006), and lithogenic gallbladder disorders (55.2% vs 34.7%, P=0.05). Women with HTG frequently had a history of hysterovariectomy (55.2% vs 19.0%, Р =0.018). Despite long-term statin therapy, they often failed to reach recommended LDL-C targets and had worse survival due to significantly higher incidence of composite endpoint (39.6% vs 22.4%, P=0.027). Further researches are necessary to find safe and effective strategy for secondary prevention in this population.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67022028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antiproliferative activity of pyrazolothiazolopyrimidine derivatives","authors":"N. Finiuk, Y. Ostapiuk, V. Hreniukh, Ya. R. Shalai, V. Matiychuk, M. Obushak, R. Stoika, A. Babsky","doi":"10.15407/UBJ90.02.025","DOIUrl":"https://doi.org/10.15407/UBJ90.02.025","url":null,"abstract":"The research aim was to test cytotoxic effects in vitro of seven novel pyrazolothiazolopyrimidine derivatives in targeting several lines of tumor and pseudo-normal mammalian cells. We demonstrated that cytotoxic effects of these derivatives depended on the tissue origin of targeted cells. Leukemia cells were found to be the most sensitive to the action of compounds 2 and 7. Compound 2 demonstrated approximately two times higher toxicity towards the multidrug-resistant sub-line of HL-60/ADR cells compared to the Doxorubicin effect. Antiproliferative action of compounds 2 and 7 dropped in the order: leukemia > melanoma > hepatocarcinoma > glioblastoma > colon carcinoma > breast and ovarian carcinoma cells. These compounds were less toxic than Doxorubicin towards the non-tumor cells. The novel pyrazolothiazolopyrimidine, compound 2, demonstrated high toxicity towards human leukemia and, of special importance, towards multidrug-resistant leukemia cells, and low toxicity towards pseudo-normal cells.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"2018-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41409244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological and analytical studies of peritoneal dialysis solutions","authors":"N. Hudz, L. Kobylinska, N. Dmytrukha, R. Korytniuk, P. Wieczorek","doi":"10.15407/UBJ90.02.034","DOIUrl":"https://doi.org/10.15407/UBJ90.02.034","url":null,"abstract":"the purpose of our work was to conduct biological and analytical studies of the peritoneal dialysis (Pd) solutions containing glucose and sodium lactate and establish correlations between cell viability of the Vero cell line and values of analytical indexes of the tested solutions. The results of this study confirm the cytotoxicity of the Pd solutions even compared with the isotonic solution of sodium chloride, which may be due to the low pH of the solutions, presence of glucose degradation products (GdPs) and high osmolarity of the solutions, and unphysiological concentrations of glucose and sodium lactate. However, it is not yet known what factors or their combination and to what extent cause the cytotoxicity of Pd solutions. In the neutral red (Nr) test the weak, almost middle (r = -0.496 and 0.498, respectively) and unexpected correlations were found between reduced viability of monkey kidney cells and increased pH of the Pd solutions and between increased cell viability and increased absorbance at 228 nm of the tested Pd solutions. these two correlations can be explained by a strong correlation (r = -0.948) between a decrease in pH and an increase in the solution absorbance at 228 nm. The opposite effect was observed in the MTT test. The weak, but expected correlations (r = 0.32 and -0.202, respectively) were found between increased cell viability and increased pH in the Pd solutions and between decreased cell viability and increased absorbance at 228 nm of the tested Pd solutions. the middle and weak correlations (r = 0.56 and 0.29, respectively) were detected between increased cell viability and increased lactate concentration in the Nr test and mtt test. the data of these correlations can be partially explained by the fact that a correlation with a coefficient r = -0.34 was found between decreased pH in the solutions and increased lactate concentration. the very weak correlations (0.138 and 0.196, respectively) were found between increased cell viability and increased glucose concentration in the Nr test and mtt test. these experimental data indicate that pH is the dominating factor, which determines almost all of the established correlations. However, the character of the correlations is quite different: the higher the pH, the greater was the cell viability in the mtt test, and conversely, the higher the pH, the lower was the cell viability in the NR test. Secondly, the unexpected correlation coefficient was determined as -0.473 between decreased cell viability in the mtt test and increased cell viability in the Nr test. moreover, this phenomenon indicates that the mitochondrial enzyme succinate dehydrogenase is more vulnerable to the action of Pd solutions than membrane permeability. Finally, we conclude that the Nr test is not suitable for comparative studies of Pd solutions which differ in pH, as it is pH dependent and does not enable the comparison of plausible cell viability.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"34-44"},"PeriodicalIF":0.0,"publicationDate":"2018-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45088451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of N-acetylcysteine on antitumor activity of doxorubicin and landomycin A in NK/Ly lymphoma-bearing mice","authors":"Yu. S. Kozak, R. Panchuk, N. Skorokhyd, L. V. Lehka, R. Stoika","doi":"10.15407/UBJ90.02.046","DOIUrl":"https://doi.org/10.15407/UBJ90.02.046","url":null,"abstract":"N-acetylcysteine (NAC) is a dietary supplement demonstrating antioxidant and liver protecting effects that is widely used in clinics. NAC is considered to possess potential therapeutic activity for health disorders characterized by generation of free oxygen radicals, as well as potential for decreasing negative side effects of various drugs. However, the mechanisms of such tissue-protective actions of NAC remain poorly understood. The main aim of this work was to study therapeutic effects of NAC applied together with the “gold standard” of chemotherapy doxorubicin (Dx) or the novel experimental drug landomycin A (LA) to mice bearing NK/Ly lymphoma. It was revealed that NAC significantly decreased the nephrotoxicity of Dx (measured as creatinine level), possessed moderate immunomodulating activity (as revealed by an increase in number of cytotoxic T-lymphocytes), and partially increased survival of NK/Ly lymphoma-bearing animals treated with Dx. On the contrary, there was little tissue-protective effect of NAC towards LA due to the weak side effects of this anticancer drug, however, the combined use of NAC and LA significantly increased survival (60+ days) of LAtreated animals with NK/Ly lymphoma. Summarizing, NAC possesses a moderate tissue-protective activity towards Dx action but lacks a major therapeutic effect. However, in the case of LA action, NAC significantly increases its anticancer activity with no impact on its negative side effects. Further studies of the molecular mechanisms underlying that activity of NAC towards the action of LA are in progress.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"46-54"},"PeriodicalIF":0.0,"publicationDate":"2018-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41959901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteoprotective effects of vitamin D(3) in diabetic mice is VDR-mediated and regulated via RANKL/RANK/OPG axis","authors":"D. Labudzynskyi, І. О. Shymanskyi, O. Lisakovska, М. М. Veliky","doi":"10.15407/UBJ90.02.056","DOIUrl":"https://doi.org/10.15407/UBJ90.02.056","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"56-65"},"PeriodicalIF":0.0,"publicationDate":"2018-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48846363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clot formation and lysis in platelet rich plasma of healthy donors and patients with resistant hypertension","authors":"I. I. Patalakh, O. V. Revka, O. Kuchmenko, O. Matova, T. Drobotko, T. Grinenko","doi":"10.15407/UBJ90.02.067","DOIUrl":"https://doi.org/10.15407/UBJ90.02.067","url":null,"abstract":"Hemostatic balance in blood is affected by numerous factors, including coagulation and fibrinolytic proteins, the wide spectrum of their inhibitors, and blood cells. Since platelets can participate in contradictory processes, they significantly complicate the whole picture. Therefore, nowadays the development of global assays of hemostasis, which can reflect the physiological process of hemostasis and can be used for point-of-care diagnosis of thrombosis, is crucial. This paper outlines a new approach we used to analyze the capabilities of clot waveform analysis tools to distinguish the response of platelet-rich plasma from healthy donors and patients with arterial hypertension caused by stimulation of coagulation and lysis (with exogenous thrombin and recombinant tissue-type plasminogen activator, respectively). In donor plasma, when the clot degradation was accompanied by 40 IU/ml of recombinant tissue-type plasminogen activator, platelets potentiated fibrinolysis more than coagulation, which ultimately shifts the overall balance to a profibrinolytic state. at the same time, for patients with hypertension, platelets, embedded in clot obtained from platelet-rich plasma, showed a weaker ability to stimulate fibrinolysis. The obtained data gives the evidence that platelets can act not only as procoagulants but also as profibrinolytics. By simultaneously amplifying coagulation and fibrinolysis, making their rates comparable, platelets would control plasma procoagulant activity, thereby regulating local hemostatic balance, the size and lifetime of the clot. Moreover, clot waveform analysis may be used to distinguish the effects of platelet-rich plasma on clotting or lysis of fibrin clots in healthy donors and patients with essential hypertension.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"67-75"},"PeriodicalIF":0.0,"publicationDate":"2018-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48303382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple two-step covalent protein conjugation to PEG-coated nanocrystals","authors":"S. Paryzhak, T. Dumych, O. Karmash, E. Bila, D. Stachowiak, M. Bański, A. Podhorodecki, R. Bilyy","doi":"10.15407/UBJ90.02.008","DOIUrl":"https://doi.org/10.15407/UBJ90.02.008","url":null,"abstract":"covering of nanocrystals (Nc) with a polyethylene glycol (PEG) envelop is a common way to increase their hydrophilicity, and compatibility with bio-systems, including increased retention time in the body. colloidal semiconductor NC, also known as quantum dots (QD), particularly benefit from covering with PEG due to passivation of the inorganic core, while maintaining physical properties of the core. Despite many advantages of covering the surface with PEG, the covalent attachment of protein to hydroxyls of PEG is complicated. Here we propose a simple two-step approach for modification of PEG residues with subsequent covalent attachment of proteins. We were able to achieve specific NC targeting by means of attached protein as well as preserve their optical parameters (fluorescence intensity) in chemical reaction conditions. In the optimized protocol, ensuring removal of chemical byproducts by dialysis, we were able to omit the need for centrifugation (usually a limiting step due to particle size). The obtained Nc-protein conjugate solutions contained 0.25x of initial unmodified NC amount, ensuring a low dilution of the sample. During all reactions the pH range was optimized to be between 6 to 8. The proposed approach can be easily modified for covalent targeting of different PEG-covered nanocomposites with proteins.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"8-12"},"PeriodicalIF":0.0,"publicationDate":"2018-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43293542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood coagulation and aortic wall integrity in rats with obesity-induced insulin resistance","authors":"O. Dziuba","doi":"10.15407/UBJ90.02.014","DOIUrl":"https://doi.org/10.15407/UBJ90.02.014","url":null,"abstract":"Obesity is an important factor in pathogenesis of disorders caused by chronic inflammation. Diet-induced obesity leads to dyslipidemia and insulin resistance (Ir) that in turn provoke the development of type 2 diabetes and cardiovascular diseases. thus, the aim of this work was to investigate the possible pro-atherogenic effects in the blood coagulation system and aortic wall of rats with obesity-induced IR. The experimental model was induced by a 6-month high-fat diet (hFD) in white rats. Blood samples were collected from 7 control and 14 obese Ir rats. Prothrombin time (Pt) and partial activated thromboplastin time (aPtt) were performed by standard methods using Coagulometer Solar СТ 2410. Fibrinogen concentration in the blood plasma was determined by the modified spectrophotometric method. Levels of protein C (PC), prothrombin and factor X were measured using specific chromogenic substrates and activa ting enzymes from snake venoms. Platelet aggregation was measured and their count determined using aggregometer Solar aP2110. the aorta samples were stained by hematoxylin and eosin according to Ehrlich. Aortic wall thickness was measured using morphometric program Image J. Statistical analysis was performed using Mann-Whitney U test. the haemostasis system was characterized by estimation of the levels of individual coagulation factors, anticoagulant system involvement and platelet reactivity. Pt and aPtt demonstrated that blood coagulation time strongly tended to decrease in obese Ir rats in comparison to the control group. It was also detec ted that 30% of studied obese IR rats had decreased factor X level, 40% had decreased level of prothrombin whereas fibrinogen concentration was slightly increased up to 3 mg/ml in 37% of obese Ir rats. a prominent decrease of anticoagulant PC in blood plasma of obese rats was detected. Obese Ir rats also had increased platelet count and higher rate of platelet aggregation in comparison to control animals. Histological analysis identified the disruption of aorta endothelium and tendency for the thickening of the aorta wall in the group with obesityinduced Ir compared to the group of control rats. Changes of individual coagulation factors were assumed as the evidence of imbalance in the blood coagulation system. Increase of fibrinogen level, drop in PC concentration and pathological platelet reactivity were taken to corroborate the development of low-grade inflammation in obese IR rats. Instant generation of small amounts of thrombin in their blood plasma is expected. Since the aorta morphology assay detected the trend of its wall to thicken and the emergence of disruptions, we assumed there were initial stages of atherosclerosis and the danger of developing atherothrombosis. We detected an increase of blood coagulability and changes in aorta morphology in rats with obesity-induced Ir which we assume indicate early development of atherosclerosis.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"90 1","pages":"14-23"},"PeriodicalIF":0.0,"publicationDate":"2018-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42058796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}