Urological Research最新文献

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Chemolysis of calcium oxalate stones: study in vitro and possible clinical application. 草酸钙结石的化学溶解:体外研究及可能的临床应用。
Urological Research Pub Date : 2012-06-01 Epub Date: 2011-11-17 DOI: 10.1007/s00240-011-0429-z
Andrey V Kustov, Alexey A Shevyrin, Alexander I Strel'nikov, Pavel R Smirnov, Vyacheslav N Trostin
{"title":"Chemolysis of calcium oxalate stones: study in vitro and possible clinical application.","authors":"Andrey V Kustov,&nbsp;Alexey A Shevyrin,&nbsp;Alexander I Strel'nikov,&nbsp;Pavel R Smirnov,&nbsp;Vyacheslav N Trostin","doi":"10.1007/s00240-011-0429-z","DOIUrl":"https://doi.org/10.1007/s00240-011-0429-z","url":null,"abstract":"<p><p>The flow cell modeling clinical conditions have been used to study the interaction between dilute chemolytic solutions and large calcium oxalate renal stones. The stone treatment with 5% disodium ethylenediaminetetraacetate aqueous solutions or citrate buffer are found not to provide notable disruption of the samples studied. The significant improvement is reached with the mixed compositions containing both natural and synthetic chelating reagents:citrate and ethylenediaminetetraacetate ions as well as an antibiotic. Description of the chemolytic irrigation, numerical results and their possible clinical application are the main topic of the present research.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0429-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30258677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Colon perforation during percutaneous renal surgery: a 10-year experience in a single endourology centre. 经皮肾手术期间结肠穿孔:在单一泌尿系统中心的10年经验。
Urological Research Pub Date : 2012-06-01 Epub Date: 2012-02-04 DOI: 10.1007/s00240-012-0464-4
Stefanos Kachrilas, Kachrilas Stefanos, Athanasios Papatsoris, Papatsoris Athanasios, Christian Bach, Bach Christian, Stylianos Kontos, Kontos Stylianos, Zaman Faruquz, Faruquz Zaman, Anuj Goyal, Goyal Anuj, Junaid Masood, Masood Junaid, Noor Buchholz, Buchholz Noor
{"title":"Colon perforation during percutaneous renal surgery: a 10-year experience in a single endourology centre.","authors":"Stefanos Kachrilas,&nbsp;Kachrilas Stefanos,&nbsp;Athanasios Papatsoris,&nbsp;Papatsoris Athanasios,&nbsp;Christian Bach,&nbsp;Bach Christian,&nbsp;Stylianos Kontos,&nbsp;Kontos Stylianos,&nbsp;Zaman Faruquz,&nbsp;Faruquz Zaman,&nbsp;Anuj Goyal,&nbsp;Goyal Anuj,&nbsp;Junaid Masood,&nbsp;Masood Junaid,&nbsp;Noor Buchholz,&nbsp;Buchholz Noor","doi":"10.1007/s00240-012-0464-4","DOIUrl":"https://doi.org/10.1007/s00240-012-0464-4","url":null,"abstract":"<p><p>The use of percutaneous renal surgery has been recently revolutionised with novel endourological instruments and techniques. However, the incidence, prevention and management of severe complications such as colon perforation still lack consensus. By presenting our 10-year experience, we would like to highlight the diagnosis and management of the rare complication of colon perforation.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0464-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30437733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Inhibition of calcium oxalate crystal deposition on kidneys of urolithiatic rats by Hibiscus sabdariffa L. extract. 芙蓉提取物对尿石症大鼠肾脏草酸钙晶体沉积的抑制作用。
Urological Research Pub Date : 2012-06-01 Epub Date: 2011-11-05 DOI: 10.1007/s00240-011-0433-3
Reena Laikangbam, M Damayanti Devi
{"title":"Inhibition of calcium oxalate crystal deposition on kidneys of urolithiatic rats by Hibiscus sabdariffa L. extract.","authors":"Reena Laikangbam,&nbsp;M Damayanti Devi","doi":"10.1007/s00240-011-0433-3","DOIUrl":"https://doi.org/10.1007/s00240-011-0433-3","url":null,"abstract":"<p><p>The present study aims at systematic evaluation of the calyces of Hibiscus sabdariffa to establish its scientific validity for anti-urolithiatic property using ethylene glycol-induced hyperoxaluria model in male albino rats. Administration of a mixture of 0.75% ethylene glycol and 2% ammonium chloride resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. The decrease in the serum calcium concentration indicates an increased calcium oxalate formation. Supplementation of aqueous extract of H. sabdariffa at different doses (250, 500 and 750 mg/kg body weight) significantly lowered the deposition of stone-forming constituents in the kidneys and serum of urolithiatic rats. These findings have been confirmed through histological investigations. Results of in vivo genotoxicity testing showed no significant chromosomal aberrations in the bone marrow cells of ethylene glycol-induced rats. The plant extracts at the doses investigated induced neither toxic nor lethal effects and are safe. It can be concluded that the calyces of H. sabdariffa are endowed with anti-urolithiatic activity and do not have genotoxic effects. Thus, it can be introduced in clinical practices and medicine in the form of orally administered syrup after further investigations and clinical trials.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0433-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40132316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
The impact of metaphylaxis of kidney stone disease in the renal function at long term in active kidney stone formers patients. 肾结石过敏反应对活动期肾结石患者长期肾功能的影响。
Urological Research Pub Date : 2012-06-01 Epub Date: 2011-08-20 DOI: 10.1007/s00240-011-0407-5
Jose A Meneses, Fernando M Lucas, Fernando C Assunção, Junia P P Castro, Rogério B Monteiro
{"title":"The impact of metaphylaxis of kidney stone disease in the renal function at long term in active kidney stone formers patients.","authors":"Jose A Meneses,&nbsp;Fernando M Lucas,&nbsp;Fernando C Assunção,&nbsp;Junia P P Castro,&nbsp;Rogério B Monteiro","doi":"10.1007/s00240-011-0407-5","DOIUrl":"https://doi.org/10.1007/s00240-011-0407-5","url":null,"abstract":"<p><p>A total of 150 patients were included in the analysis. Through chart review, we estimated glomerular filtration rate in the first visit and, at least, 5 years later. All patients were divided into two groups: (1) metaphylaxis adherents (n = 74) and (2) metaphylaxis non-adherents (n = 76). We followed all patients for at least 5 years. The Scr percentage of patients and GFR <60 mL/min was compared between groups. Variables were compared between groups using t test, χ(2) tests, odds ratios with 95% confidence intervals. There were no differences at baseline between groups. After 5 years of follow-up, GFR was 77.9 mL/min in non-adherent-metaphylaxis group and in the adherent-metaphylaxis group was 87.3 mL/min, with p value of 0.02. After 5 years of follow-up, we had a GFR <60 mL/min in the adherent-metaphylaxis group (4.89 vs. 21.95%) with p value of 0.001 and OR = 5.36; IC-95% = 1.95-14.8. Metaphylaxis of kidney stone disease could prevent chronic kidney disease.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0407-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30091382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Is the fasting calcium/creatinine a bone resorption marker in patients with calcium renal stones? 空腹钙/肌酐是钙性肾结石患者骨吸收指标吗?
Urological Research Pub Date : 2012-06-01 Epub Date: 2011-12-04 DOI: 10.1007/s00240-011-0441-3
Miguel Angel Arrabal-Polo, Miguel Arrabal-Martin, Antonio Poyatos-Andujar, Encarnacion Cardenas-Grande, Sergio Merino-Salas, Armando Zuluaga-Gomez
{"title":"Is the fasting calcium/creatinine a bone resorption marker in patients with calcium renal stones?","authors":"Miguel Angel Arrabal-Polo,&nbsp;Miguel Arrabal-Martin,&nbsp;Antonio Poyatos-Andujar,&nbsp;Encarnacion Cardenas-Grande,&nbsp;Sergio Merino-Salas,&nbsp;Armando Zuluaga-Gomez","doi":"10.1007/s00240-011-0441-3","DOIUrl":"https://doi.org/10.1007/s00240-011-0441-3","url":null,"abstract":"<p><p>Osteoporosis and osteopenia are an important endocrine-metabolic disease that affect women and men from a certain age and it has a high risk and health cost. The aim of this short communication is to show that fasting calcium/creatinine ratio in patients with calcium stones is a marker of bone resorption.We studied 180 patients with renal stones with calcium composition and the relationship of them between the calcium/creatinine in urine after 8 h of fasting with bone densitometry (T-score) and values of bone resorption marker β-crosslaps (ng/ml). The Pearson correlation test was applied for the analysis of linear correlations between quantitative variables.We have observed a statistically significant positive linear correlation between the fasting calcium/creatinine and serum and β-crosslaps (R = 0.534, p < 0.0001) and a statistically significant negative linear correlation between fasting calcium/creatinine and T-score of bone densitometry in hip (R = -0.237, p = 0.002), femoral neck (R = -0.217, p = 0.009) and lumbar spine (R = 0.292, p = 0.001).The fasting ratio calcium/creatinine in urine is associated with increased levels of β-crosslaps marker and therefore may be useful as a marker of bone resorption in these patients.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0441-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30300937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Role of combined use of potassium citrate and tamsulosin in the management of uric acid distal ureteral calculi. 柠檬酸钾联合坦索罗辛在尿酸性输尿管远端结石治疗中的作用。
Urological Research Pub Date : 2012-06-01 Epub Date: 2011-08-21 DOI: 10.1007/s00240-011-0406-6
Osama El-Gamal, Mohamed El-Bendary, Maged Ragab, Mohamed Rasheed
{"title":"Role of combined use of potassium citrate and tamsulosin in the management of uric acid distal ureteral calculi.","authors":"Osama El-Gamal,&nbsp;Mohamed El-Bendary,&nbsp;Maged Ragab,&nbsp;Mohamed Rasheed","doi":"10.1007/s00240-011-0406-6","DOIUrl":"https://doi.org/10.1007/s00240-011-0406-6","url":null,"abstract":"<p><p>In this article, we investigated the effect of the combined use of tamsulosin and potassium citrate (Uralyt-U(®)) for the treatment of uric acid stones in the distal ureter. The study was designed as a prospective, double blind randomized controlled trial. A total of 191 adult patients with radiolucent distal ureteral calculi were recruited. We included patients with solitary stones ≥5 mm with mild or moderate hydronephrosis and a normal contralateral tract. The patients were randomized into four equal groups (the placebo, tamsulosin, Uralyt-U(®), and the combined treatment groups). The patients were treated for a maximum duration of 4 weeks or until stone expulsion. The stone size in all groups ranged from 5 to 11 mm (7.69 ± 1.7 mm). The total expulsion rate of the stones was significantly lower in the control group (26.1%) compared with that of any of the other three groups (68.8, 58.7, and 84.8% respectively) (P < 0.05). Meanwhile, the difference between the Uralyt-U(®) group and the combined treatment group was also statistically significant (P < 0.05). When we studied the patients with stones >8 mm as a separate subgroup to find the effect of the used drugs on the relatively large stones, we detected that the expulsion rate of these stones was significantly higher in the patients who received the combined treatment in comparison with any of the other three groups (P < 0.05). In conclusion, the use of urinary alkalization with tamsulosin can increase the frequency of spontaneous passage of distal ureteral uric acid stones especially those of 8-11 mm.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0406-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30091261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 33
Percutaneous nephrolithotomy: keeping the bridge for one night. 经皮肾镜取石术:留桥一晚。
Urological Research Pub Date : 2012-06-01 Epub Date: 2012-01-19 DOI: 10.1007/s00240-011-0445-z
Cengiz Kara
{"title":"Percutaneous nephrolithotomy: keeping the bridge for one night.","authors":"Cengiz Kara","doi":"10.1007/s00240-011-0445-z","DOIUrl":"https://doi.org/10.1007/s00240-011-0445-z","url":null,"abstract":"","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0445-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30398095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Updated epidemiologic study of urolithiasis in Turkey II: role of metabolic syndrome components on urolithiasis. 土耳其尿石症最新流行病学研究II:代谢综合征成分在尿石症中的作用。
Urological Research Pub Date : 2012-06-01 Epub Date: 2011-12-27 DOI: 10.1007/s00240-011-0447-x
Murat Binbay, Emrah Yuruk, Tolga Akman, Erhan Sari, Ozgur Yazici, Ibrahim Mesut Ugurlu, Yalcın Berberoglu, Ahmet Yaser Muslumanoglu
{"title":"Updated epidemiologic study of urolithiasis in Turkey II: role of metabolic syndrome components on urolithiasis.","authors":"Murat Binbay,&nbsp;Emrah Yuruk,&nbsp;Tolga Akman,&nbsp;Erhan Sari,&nbsp;Ozgur Yazici,&nbsp;Ibrahim Mesut Ugurlu,&nbsp;Yalcın Berberoglu,&nbsp;Ahmet Yaser Muslumanoglu","doi":"10.1007/s00240-011-0447-x","DOIUrl":"https://doi.org/10.1007/s00240-011-0447-x","url":null,"abstract":"<p><p>The components of metabolic syndrome, such as obesity, hypertension, and diabetes, are thought to be associated with urolithiasis. However, there are few large-scale studies that have examined the association between metabolic syndrome and urolithiasis, which prompted us to study and evaluate the relationship between metabolic syndrome components and urolithiasis in a nationwide survey, using the cross-sectional study conducted by a professional investigation company, with 2,468 enrolled participants, aged between 18 and 70 years, from 33 provinces in Turkey. Participants were interviewed face-to-face by medical faculty students. Participants with a history of urolithiasis (Group 1) were compared with participants without a history of urolithiasis (Group 2) in terms of hypertension, diabetes, body-mass index (BMI), waist size, and trouser size using Chi-square and odds ratio tests. Of the 2,468 participants, 274 (11.1%) reported a history of urinary stone disease diagnosed by a physician. The percentage of participants with hypertension along with urolithiasis was significantly higher than that in participants without urolithiasis (16.9 and 34.3%, p 0.000, OR 3.0). The percentage of participants with diabetes in groups 1 and 2 was 14.2 and 9%, respectively (p 0.001, OR 1.83). The mean BMI was 27.2 and 25.2, respectively (p 0.01). Participants with a BMI >30 had a 2.2-fold increased risk of having urolithiasis. The mean waist size was significantly greater in participants with urolithiasis (p 0.000). Those with a waist size >100 cm had a 1.87-fold increased risk of having urolithiasis. The mean trouser size was also significantly larger in those participants who were stone formers (p 0.003). The results indicate that metabolic syndrome components are important factors in the development of urolithiasis.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0447-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30350946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Efficiency of percutaneous nephrolithotomy in pediatric patients using adult-type instruments. 经皮肾镜取石术在儿科患者中的应用效果。
Urological Research Pub Date : 2012-06-01 Epub Date: 2011-08-05 DOI: 10.1007/s00240-011-0408-4
Bayram Dogan, Ali Fuat Atmaca, Abdullah Erdem Canda, Abidin Egemen Isgoren, Ziya Akbulut, Mevlana Derya Balbay
{"title":"Efficiency of percutaneous nephrolithotomy in pediatric patients using adult-type instruments.","authors":"Bayram Dogan,&nbsp;Ali Fuat Atmaca,&nbsp;Abdullah Erdem Canda,&nbsp;Abidin Egemen Isgoren,&nbsp;Ziya Akbulut,&nbsp;Mevlana Derya Balbay","doi":"10.1007/s00240-011-0408-4","DOIUrl":"https://doi.org/10.1007/s00240-011-0408-4","url":null,"abstract":"<p><p>To evaluate the efficiency of percutaneous nephrolithotomy using adult-type instruments in children with kidney stones. Between September 2004 and October 2009, 18 children (19 renal units) underwent percutaneous nephrolithotomy using adult-type instruments. Following percutaneous access under fluoroscopy, 20-30F tract dilatation was performed (1,92,427F), and lithotripters were used. Postoperatively, kidney-ureter-bladder X-ray and antegrade pyelography were performed to evaluate residual stones and contrast passage to the bladder. 8 boys and 10 girls with a mean age of 9.8 ± 4.56 years were evaluated. Mean stone burden was 338 ± 196.21 mm². Stones were located in the left and right kidneys in 16 (84.2%) and 3 (16.8%) patients, respectively. Horse-shoe kidney was present in one patient. Mean operation (including cystoscopy) and fluoroscopy times were 106 ± 49.60 and 5.2 ± 2.14 min, respectively. Postoperatively, 10(52.6%) patients were stone free and 4 (21.1%) patients had clinically insignificant stones. Saline extravasation developed in three patients and surgery was aborted in one patient. Stone fragments migrated into the ureter in two patients and managed by additional endourological interventions. Nephrostomy catheters were kept for a mean of 2.6 ± 1.12 days. Four patients required blood transfusion due to bleeding. Postoperative fever of <39°C developed in five patients and >39°C in one patient. Mean hospitalization time was 5.3 ± 3.12 days. Overall, 73.7% of our patients were stone free, including patients with clinically insignificant stones. Particularly in children with a high-stone burden, the use of adult-type instruments might have a positive impact on stone-free rate, operation time and fluoroscopy time without increasing the complication rate.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0408-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29911307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin. 原花青素对多重耐药大肠杆菌粘附的抑制作用。
Urological Research Pub Date : 2012-04-01 Epub Date: 2011-06-19 DOI: 10.1007/s00240-011-0398-2
Ashish Gupta, Mayank Dwivedi, Abbas Ali Mahdi, G A Nagana Gowda, Chunni Lal Khetrapal, Mahendra Bhandari
{"title":"Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin.","authors":"Ashish Gupta,&nbsp;Mayank Dwivedi,&nbsp;Abbas Ali Mahdi,&nbsp;G A Nagana Gowda,&nbsp;Chunni Lal Khetrapal,&nbsp;Mahendra Bhandari","doi":"10.1007/s00240-011-0398-2","DOIUrl":"https://doi.org/10.1007/s00240-011-0398-2","url":null,"abstract":"<p><p>Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and (13)C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70% with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10-50 µg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-011-0398-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29948621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 59
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