Urological Research最新文献

{"title":"A simple and rapid colorimetric method for determination of phytate in urine.","authors":"Antonia Costa-Bauza,&nbsp;F Grases,&nbsp;I Gomila,&nbsp;A Rodriguez,&nbsp;R M Prieto,&nbsp;F Tur","doi":"10.1007/s00240-012-0473-3","DOIUrl":"https://doi.org/10.1007/s00240-012-0473-3","url":null,"abstract":"<p><p>Phytate is a natural product present in urine and biological fluids that is associated with health benefits, such as the prevention of calcium renal stone formation. The available methods for phytate analysis in urine all require elaborate instrumentation and cannot be routinely applied in clinical laboratories. Here, we describe a simple procedure for urinary phytate determination, employing colorimetric detection. Our method requires purification and preconcentration of phytate via solid-phase extraction prior to colorimetric detection employing Fe(III)-thiocyanate. The working linear range of the assay is 0-5 μM phytate. The limit of detection is 0.055 μM. The relative standard deviation obtained upon assay of samples containing 2 μM phytate was 3.5 %. Several urine samples were analyzed using an alternative method based on the detection of phosphorus; the results of the two assays were comparable. Our novel method of phytate analysis in human urine is simple, rapid (3 h for 10 samples), accurate, precise, reliable, and highly sensitive. The assay can be run in most analytical laboratories and does not require sophisticated instrumentation.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0473-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30551762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicinal plants extracts affect virulence factors expression and biofilm formation by the uropathogenic Escherichia coli.","authors":"Dorota Wojnicz,&nbsp;Alicja Z Kucharska,&nbsp;Anna Sokół-Łętowska,&nbsp;Marta Kicia,&nbsp;Dorota Tichaczek-Goska","doi":"10.1007/s00240-012-0499-6","DOIUrl":"https://doi.org/10.1007/s00240-012-0499-6","url":null,"abstract":"<p><p>Medicinal plants are an important source for the therapeutic remedies of various diseases including urinary tract infections. This prompted us to perform research in this area. We decided to focus on medicinal plants species used in urinary tract infections prevention. The aim of our study was to determine the influence of Betula pendula, Equisetum arvense, Herniaria glabra, Galium odoratum, Urtica dioica, and Vaccinium vitis-idaea extracts on bacterial survival and virulence factors involved in tissue colonization and biofilm formation of the uropathogenic Escherichia coli rods. Qualitative and quantitative analysis of plant extracts were performed. Antimicrobial assay relied on the estimation of the colony forming unit number. Hydrophobicity of cells was established by salt aggregation test. Using motility agar, the ability of bacteria to move was examined. The erythrocyte hemagglutination test was used for fimbriae P screening. Curli expression was determined using YESCA agar supplemented with congo red. Quantification of biofilm formation was carried out using a microtiter plate assay and a spectrophotometric method. The results of the study indicate significant differences between investigated extracts in their antimicrobial activities. The extracts of H. glabra and V. vitis-idaea showed the highest growth-inhibitory effects (p < 0.05). Surface hydrophobicity of autoaggregating E. coli strain changed after exposure to all plant extracts, except V. vitis-idaea (p > 0.05). The B. pendula and U. dioica extracts significantly reduced the motility of the E. coli rods (p < 0.05). All the extracts exhibited the anti-biofilm activity.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0499-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30853342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The technical details of treatment of kidney stone in children.","authors":"Abdulkadir Tepeler,&nbsp;Musfata Gunes,&nbsp;Fatih Elbir,&nbsp;Tolga Akman,&nbsp;Huseyin Kilincaslan","doi":"10.1007/s00240-012-0510-2","DOIUrl":"https://doi.org/10.1007/s00240-012-0510-2","url":null,"abstract":"","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0510-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30911161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The double wire technique: an alternative method for difficult ureteroscopic access.","authors":"Yakup Bostanci,&nbsp;Ender Ozden,&nbsp;Fatih Atac,&nbsp;Yarkin Kamil Yakupoglu","doi":"10.1007/s00240-012-0491-1","DOIUrl":"https://doi.org/10.1007/s00240-012-0491-1","url":null,"abstract":"<p><p>We read with great interest the study report by Ji et al. (Urol Res, doi: 10.1007/s00240-012-0476-0 , 2012) about the impact of the intentional ureteral stenting on the success rate of calculus extraction by second ureteroscopy, when the initial ureteroscopy failed. Actually, sometimes ureteral stone treatment pose challenging problems for the urologists. This study has filled an important gap on this field. If flexible ureteroscopy and laser lithotripter are not available pre-existing stent may improve the success rate of a subsequent ureteroscopy for impacted ureteral stones. Because, post-stent ureteroscopy has the advantage of working through a dilated ureter. We would like to add some additional comments and suggestions in addition to the authors' discussion, which is noteworthy in several respects.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0491-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30736139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of a 4.5 F semi-rigid ureteroscope with a 7.5 F rigid ureteroscope in the treatment of ureteral stones in preschool-age children.","authors":"Murat Atar,&nbsp;Ahmet Ali Sancaktutar,&nbsp;Necmettin Penbegul,&nbsp;Haluk Soylemez,&nbsp;Mehmet Nuri Bodakci,&nbsp;Namik Kemal Hatipoglu,&nbsp;Yasar Bozkurt,&nbsp;Suleyman Cakmakci","doi":"10.1007/s00240-012-0489-8","DOIUrl":"https://doi.org/10.1007/s00240-012-0489-8","url":null,"abstract":"<p><p>The aim of this study was to compare the success and complication rates of a 4.5 F ureteroscope with a 7.5 F ureteroscope in the treatment of urolithiasis in preschool-age children. We retrospectively reviewed 69 ureteroscopy (URS) procedures in a pediatric population (40 boys, 29 girls). We divided the patients into two groups according to the type of ureteroscope used: group 1 (n = 42, Storz 7.5 F) and group 2 (n = 27, Wolf 4.5 F). We statistically compared all the procedures performed in both groups regarding patient age, complication rates, whether the procedure was therapeutic, and whether we used a guidewire. Additionally, in cases with ureteral stones, we also compared the stone clearance rate and the necessity of X-ray imaging between the two groups. The mean patient age was 56.04 months in group 1 and 47.48 months in group 2 (p = 0.057). The stone-free rate was 78.6 % in group 1 and 92.6 % in group 2 (p > 0.05). However, when we compared the stone-free rates for patients younger than 3 years, the rate was 66.7 % in group 1 and 93.8 % in group 2 (p < 0.05). The difference was not statistically significant for patients between the ages of 4 and 7 years. The success and failure rates revealed better outcomes for treatment of ureteral stones with a 4.5 F ureteroscope. We recommend the use of the mini-ureteroscope, especially in infants and preschool-age children.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0489-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30752541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naftopidil and tolterodine in the medical expulsive therapy for intramural ureteral stones: a prospective randomized study.","authors":"Jian Lin Lu,&nbsp;Qing Lai Tang,&nbsp;Fa De Liu,&nbsp;Ju Hong Hui","doi":"10.1007/s00240-012-0498-7","DOIUrl":"https://doi.org/10.1007/s00240-012-0498-7","url":null,"abstract":"<p><p>We performed a randomized, prospective study to assess the possible role of combined naftopidil and tolterodine for facilitating the spontaneous expulsion of intramural ureteral stones. A total of 76 patients with intramural ureter stones were included in the study from December 2007 to February 2011. Patients were randomized to one of three treatment groups. Group A patients received naftopidil 25 mg/day, group B patients received naftopidil 25 mg/day plus tolterodine 2 mg (twice a day), and group C patients received tolterodine 2 mg (twice a day). Both groups were followed up for 2 weeks. The stone expulsion rate and time and the number of pain episodes were obtained. Subjects rated the urgency associated with each micturition using the Urinary Sensation Scale (USS). Pain descriptions were recorded by the patients using the visual analog scale (VAS). A significant difference was shown for the expulsion rate between the group C and the other two groups (P < 0.001 by log rank test). In groups A, B and C, the mean number of pain episodes was 2.25 ± 0.90, 1.38 ± 1.37 and 1.54 ± 1.18, respectively. The USS score for groups A, B and C at 3 days was 2.32 ± 0.55, 1.4 ± 0.58 and 1.34 ± 0.49, respectively. It was 1.75 ± 0.44, 1.2 ± 0.41 and 1.22 ± 0.42, respectively, at 7 days. On the other hand, a statistically significant difference was found between groups A and B, and groups A and C in relation to the visual analog scale score on days 3 and 7, respectively. Treatment with naftopidil and tolterodine appears to be beneficial in intramural ureteral stones clearance, particularly in the intramural ureter with symptoms of vesical irritability.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0498-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30860658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unique surface and internal structure of struvite crystals formed by Proteus mirabilis.","authors":"Jolanta Prywer, Agnieszka Torzewska, Tomasz Płociński","doi":"10.1007/s00240-012-0501-3","DOIUrl":"10.1007/s00240-012-0501-3","url":null,"abstract":"<p><p>Crystallization of struvite from artificial urine in the presence of Proteus mirabilis microorganisms depends strongly on pH value. At small value of pH, struvite yields crystals of coffin-like habit with very specific structure. The analysis using scanning electron microscopy shows that the crystals possess well-defined faces, but higher magnifications show very specific structuration as if the crystals were built from small three-dimensional subunits. The possible role of microorganisms in the formation of such a structuration is analyzed. At higher pH value, the crystals exhibit dendritic growth with main trunk and branches. Although the formation mechanism of the specific structuration as well as dendritic structures is unknown, the nature of forces for such an alignment is analyzed. The revealed porous internal structure of struvite is also analyzed. The investigations provide evidence for the importance of biological regulation in crystallization process.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30849814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperoxaluric rats do not exhibit alterations in renal expression patterns of Slc26a1 (SAT1) mRNA or protein.","authors":"Robert W Freel,&nbsp;Marguerite Hatch","doi":"10.1007/s00240-012-0480-4","DOIUrl":"https://doi.org/10.1007/s00240-012-0480-4","url":null,"abstract":"<p><p>Little is known about oxalate transport in renal epithelia under basal conditions, let alone in hyperoxaluria when the capacity for renal oxalate excretion is increased. Sulfate anion transporter 1 (SAT1, Slc26a1) is considered to be a major basolateral anion-oxalate exchanger in the proximal tubule and we hypothesized its expression may correlate with urinary oxalate excretion. We quantified changes in the renal expression of SAT1 mRNA and protein in two rat models, one with hyperoxaluria (HYP) and one with renal insufficiency (HRF) induced by hyperoxaluria. The hyperoxaluria observed in the HYP group could not simply be ascribed to changes in SAT1 mRNA or protein abundance. However, when hyperoxaluria was accompanied by renal insufficiency, significant reductions in SAT1 mRNA and protein were detected in medullary and papillary tissue. Together, the results indicate that transcriptional modulation of the SAT1 gene is not a significant component of the hyperoxaluria observed in these rat models.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0480-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30608000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changing to a loop-type ureteral stent decreases patients' stent-related symptoms.","authors":"Takashi Kawahara,&nbsp;Hiroki Ito,&nbsp;Hideyuki Terao,&nbsp;Takehiko Ogawa,&nbsp;Hiroji Uemura,&nbsp;Yoshinobu Kubota,&nbsp;Junichi Matsuzaki","doi":"10.1007/s00240-012-0500-4","DOIUrl":"https://doi.org/10.1007/s00240-012-0500-4","url":null,"abstract":"<p><p>The first indwelling ureteral splint was described in 1967. A ureteral stent can cause unpleasant side effects, such as urinary frequency, urgency, incontinence, hematuria, bladder pain and flank pain, which have a negative impact on a patient's quality of life. It is necessary to minimize the amount of material in the bladder in order to decrease stent-related symptoms. This study investigated the stent-related symptoms after changing from a double pigtail to a loop-type ureteral stent in the same patient group. This study followed 25 patients who underwent ureteral stent exchange from double pigtail to loop-type ureteral stent between September 2009 and February 2010. Ureteral stents were exchanged using topical, conscious sedation and general anesthesia for the various procedures including stent exchange, before/after shock wave lithotripsy and before/after ureteroscopy. The stent length was selected to be the same as whole ureteral length and the caliber based on the previous stent. A self-administered stent-related symptom questionnaire was used to assess stent-related symptoms in comparison to the previous double-pigtail stents. A total of 25 patients with a median age of 56.5 years underwent ureteral stent exchange. All patients had stone disease except two patients who had ureteral stricture. Almost all of stent-related symptoms without nocturia showed a significantly lower score with the loop-type ureteral stent than in double-pigtail stent. None of the patients experienced urinary tract infection either before or after undergoing ureteral stent exchange. Changing to loop-type ureteral stent significantly decreased ureteral stent-related symptoms.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0500-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30839705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urgent shock wave lithotripsy as first-line treatment for ureteral stones: a meta-analysis of 570 patients.","authors":"Stefano C M Picozzi,&nbsp;Cristian Ricci,&nbsp;Maddalena Gaeta,&nbsp;Stefano Casellato,&nbsp;Robert Stubinski,&nbsp;Dario Ratti,&nbsp;Giorgio Bozzini,&nbsp;Luca Carmignani","doi":"10.1007/s00240-012-0484-0","DOIUrl":"https://doi.org/10.1007/s00240-012-0484-0","url":null,"abstract":"<p><p>The rationale for the use of immediate shock wave lithotripsy (SWL) after a renal colic episode is to obtain maximum stone clearance in the shortest possible time with associated early detection of lithotripsy failures which can be treated with auxiliary procedures. The aim of this meta-analysis is to understand the role of this treatment option in the emergency setting as first-line treatment and to compare such an immediate procedure to a delayed one in terms of stone-free and complication rates. A bibliographic search covering the period from January 1995 to September 2010 was conducted in PubMed, MEDLINE and EMBASE. Database searches yielded 48 references. This analysis is based on the seven studies that fulfilled the predefined inclusion criteria. A total of 570 participants were included. The number of participants in each survey ranged from 16 to 200 (mean 81.42). Six studies were published after 2000 and one in the 1990s. All studies reported participants' age with mean of 40.9 years, and range between 11 and 88 years. All patients presented with unilateral lithiasis, as such the number of total stones treated was 570. Mean stone diameter ranged between 6.38 and 8.45 mm. According to the logistic regression applied stone-free rates were 79 % (61-95) for the proximal ureter, 78 % (69-88) for the mid ureter, 79 % (74-84) for the distal ureter and 78 % (75-82) for overall. Stone-free rates do not evidence a statistically significant difference compared to those described in the AUA and EAU guidelines for elective management. SWL management of ureteral stones in an emergency setting is completely lacking in the international guidelines and they results disperse in the literature in few works. According to our meta-analysis, immediate SWL for a stone-induced acute renal colic seems to be a safe treatment with high success rate. This evidence will be validated by further randomized studies, with a larger series of patients.</p>","PeriodicalId":23412,"journal":{"name":"Urological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00240-012-0484-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30690760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}