Transfusion Clinique et Biologique最新文献_第4页

Study of the antigenic characteristics of red blood cells units and their sickle cell disease recipients and the G6PD activity of transfused red blood cells units 研究红细胞单位及其镰状细胞病受体的抗原特征以及输血红细胞单位的 G6PD 活性。

IF 1.4 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-03-09 DOI: 10.1016/j.tracli.2024.03.002

{"title":"Study of the antigenic characteristics of red blood cells units and their sickle cell disease recipients and the G6PD activity of transfused red blood cells units","authors":"","doi":"10.1016/j.tracli.2024.03.002","DOIUrl":"10.1016/j.tracli.2024.03.002","url":null,"abstract":"<div><h3>Introduction</h3><p>Transfusion has a central place in the treatment of patients with sickle cell disease (SCD). Matching blood groups of red blood cell (RBC) units with the blood groups of the patient is essential to prevent alloimmunization and delayed hemolytic transfusion reaction. African ancestry donors have the best phenocompatibility with patients of the same origin, however their RBCs may present characteristic that can alter quality of the unit such as glucose-6-phosphate dehydrogenase (G6PD) deficiency. The objective is to analyze transfusion protocol, immunization rate and mismatch situations of SCD recipients and to evaluate the frequency of G6PD deficiency in RBCs units from African ancestry donors.</p></div><div><h3>Methods</h3><p>Samples of units transfused to SCD patients were analyzed. Transfusion data were collected from institutional databases. The activity of G6PD was measured in the segment of the RBC units.</p></div><div><h3>Results</h3><p>A total of 98 segments of units transfused to 37 SCD recipients in 41 transfusions episodes was collected. Among patients, 35.1% (<em>n</em> = 13) had no antibodies; 10.8% (<em>n</em> = 4) had antibodies against Fy<sup>a</sup>/Fy<sup>b</sup>, Jk<sup>a</sup>/Jk<sup>b</sup>, M/N, S/s; 21.6% (<em>n</em> = 8) against RH/K antigens. In all cases, the protocols were in line with the recommendations. G6PD deficiency was observed in 9 units, that were all collected from Afro-Caribbean donors.</p></div><div><h3>Conclusion</h3><p>The transfusion protocol is established to prevent immunological reactions due to disparities in blood group antigens between donors and SCD recipients. However, the units of African ancestry donors, which allowed the best compatibility, displayed a high rate of G6PD deficiency. The storage and recovery impact of this deficiency must be evaluated.</p></div>","PeriodicalId":23262,"journal":{"name":"Transfusion Clinique et Biologique","volume":"31 3","pages":"Pages 130-134"},"PeriodicalIF":1.4,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How we forecast tomorrow’s haemovigilance 我们如何预测未来的血液监测？

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-03-07 DOI: 10.1016/j.tracli.2024.03.001

Erica M Wood , Barbee I Whitaker , Mary Townsend , Shruthi Narayan

引用次数: 0

In memoriam 悼念

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-28 DOI: 10.1016/j.tracli.2024.02.002

Olivier Garraud (Editor in Chief) , Pierre Tiberghien , Jacques Chiaroni , France Pirenne

引用次数: 0

Expression of microRNAs during apheresis platelet storage up to day 14 in a blood bank in China 中国一家血库在无创血小板储存至第 14 天期间的 microRNA 表达。

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-07 DOI: 10.1016/j.tracli.2024.02.001

Jie Shi , Anqi Xu , Jun Ai , Jin Chen , Ying Luo

{"title":"Expression of microRNAs during apheresis platelet storage up to day 14 in a blood bank in China","authors":"Jie Shi , Anqi Xu , Jun Ai , Jin Chen , Ying Luo","doi":"10.1016/j.tracli.2024.02.001","DOIUrl":"10.1016/j.tracli.2024.02.001","url":null,"abstract":"<div><h3>Background</h3><p>Storage affects platelet microRNAs (miRNAs); discussing miRNA expression differences in apheresis platelets after varied storage periods is important for developing platelet quality measurement tools and identifying platelet storage lesion biomarkers. To our knowledge, the difference of MicroRNA expression profile in up to 14-day storage apheresis platelets has less relevant reports.</p></div><div><h3>Study design and methods</h3><p>Apheresis platelet bags from three donors were collected, divided into six groups, and stored for 1, 3, 5, 7, 9, and 14 days. miRNA expression was determined using quantitative reverse transcription polymerase chain reaction. Differentially expressed miRNAs were screened using RNA sequencing.</p></div><div><h3>Results</h3><p>MiRNA expression profiles showed that the six treatment groups generally highly expressed hsa-let-7 family, hsa-miR-26a-5p, hsa-miR-92a-3p, hsa-miR-199, and hsa-miR-103a-3p. A total of 15 miRNAs in the top 10 known miRNAs of the six groups were highly expressed. Time series analyses for the trend classification of 944 differentially expressed miRNAs indicated 43 genes with 14 trend changes. Hsa-miR-223-3p, hsa-miR-181a-5p, hsa-miR-4433b-5p, hsa-miR-22-3p, and hsa-miR-30c-5p were selected, and the qRT-PCR results also showed that they were significantly reduced under standard blood bank condition.</p></div><div><h3>Discussion</h3><p>Expression of microRNAs lays the foundation for further research on apheresis platelet storage lesions. Based on our results from information analysis and miRNA target gene prediction, we suggest hsa-miR-30c-5p as a biomarker of the quality and viability of apheresis platelets during storage in blood banks.</p></div>","PeriodicalId":23262,"journal":{"name":"Transfusion Clinique et Biologique","volume":"31 2","pages":"Pages 95-101"},"PeriodicalIF":1.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1246782024000375/pdfft?md5=6f6e4564ad5f819680c6ee387c9aa813&pid=1-s2.0-S1246782024000375-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum du résumé «P-119 Phénotypage étendu aux systèmes de groupes sanguins rhésus, KIDD, DUFFY, KELL, MNSS, LEWIS, LUTHERAN, P des donneurs de sang de groupe sanguin O rhésus positif au centre national de transfusion sanguine du Sénégal» [Transfusion Clinique et Biologique 30(Supplement 1) (2023) S106-S107] 对摘要 "P-119 表型分析扩展到恒河猴、KIDD、DUFFY、KELL、MNSS、LEWIS、LUTHERAN、塞内加尔国家输血中心的 P 血型 O 型恒河猴阳性献血者 "的更正[输血临床与生物 30(Supplement 1) (2023) S106-S107]。

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-01 DOI: 10.1016/j.tracli.2023.12.005

Harouna Ousmane Sow , Macoura Gadji , Oumar Atoumane Kane , Alioune Badara Senghor , Tandakha Ndiaye Dièye

引用次数: 0

Acknowledging our reviewers 2023 感谢审查员 2023

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-01 DOI: 10.1016/S1246-7820(24)00033-8

引用次数: 0

Effect of vibration of the vortex mixer on the red blood cells 旋涡混合器振动对红细胞的影响。

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-01 DOI: 10.1016/j.tracli.2023.11.003

Chuang Xiang, Liang Wang

{"title":"Effect of vibration of the vortex mixer on the red blood cells","authors":"Chuang Xiang, Liang Wang","doi":"10.1016/j.tracli.2023.11.003","DOIUrl":"10.1016/j.tracli.2023.11.003","url":null,"abstract":"<div><h3>Purposes</h3><p>Red blood cells (RBCs) are often subject to vibration during processing, transfusion, and transport. Further research is necessary to understand the effects of vibration on human RBCs and to reduce experimental deviations caused by device vibration.</p></div><div><h3>Methods</h3><p>Flow cytometry was used in this study to observe the cytokine expression of IgG and IgA and deformation of human red blood cells affected by the vibration of a vortex mixer with varying frequency (750 rpm and 1500 rpm), duration (5 min and 10 min), and container volume (96 well plate and 48 well plate).</p></div><div><h3>Results</h3><p>The size of RBCs in duration of 10 min is obviously smaller than the duration of 5 min. The 10-minute duration led to visibly smaller RBC sizes compared to the 5-minute duration. There was little effect on the size of RBCs in the 10-minute groups from differences in frequency and container volume. However, decreased RBC size can be observed in the 5-minute groups, where frequency is increased or container volume is decreased. Echinocytes were present in photomicrographs of all 10-minute groups, but microstructure of the RBCs was not impacted by vortex mixer vibration. The elevated frequency or reduced container volume results in an increased cytokine expression of IgG within the 5-minute groupings.</p></div><div><h3>Conclusion</h3><p>It can be inferred that vibration must not be overlooked due to its potential impact on the shape and cytokine expression of RBCs. Hence, the inclusion of vibration must be taken into consideration in experiments and devices pertaining to RBCs.</p></div>","PeriodicalId":23262,"journal":{"name":"Transfusion Clinique et Biologique","volume":"31 1","pages":"Pages 13-18"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1246782023005463/pdfft?md5=934d074120c374e7f07d8ec0fb47b894&pid=1-s2.0-S1246782023005463-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum du résumé «P-061 Quantification des anticorps IgM et IgG anti SARS-COV-2 chez les étudiants de l’Université Cheikh Anta Diop de Dakar» [Transfusion Clinique et Biologique 30(Supplement 1) (2023) S84] 对摘要 "P-061 达喀尔谢赫-安塔-迪奥普大学学生 SARS-COV-2 IgM 和 IgG 抗体定量 "的更正 [Transfusion Clinique et Biologique 30(Supplement 1) (2023) S84]。

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-01 DOI: 10.1016/j.tracli.2023.12.004

Harouna Ousmane Sow , Macoura Gadji , Mamadou Woury Diallo , Oumar Atoumane Kane , Khadidiatou Kébé , Alioune Badara Senghor

引用次数: 0

Application of anti-D immunoglobulin in D-negative pregnant women in China 抗d免疫球蛋白在中国d阴性孕妇中的应用。

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-01 DOI: 10.1016/j.tracli.2023.11.002

Lihui Fu, Chunya Ma, Yang Yu

{"title":"Application of anti-D immunoglobulin in D-negative pregnant women in China","authors":"Lihui Fu, Chunya Ma, Yang Yu","doi":"10.1016/j.tracli.2023.11.002","DOIUrl":"10.1016/j.tracli.2023.11.002","url":null,"abstract":"<div><p>This article summarizes the current situation of anti-D immunoglobulin (anti-D-Ig) use in RhD-negative pregnant women at home and abroad. The article describes the concept, research and development history, and domestic and foreign applications of anti-D-Ig and points out that anti-D-Ig has not been widely used in China, mainly due to reasons such as unavailability in the domestic market and non-standard current application strategies. The article focuses on analyzing the genetic and immunological characteristics of RhD-negative populations in China. The main manifestations were that the total number of hemolytic disease of the newborn (HDN) relatively high and D variant type. In particular, there are more Asian-type DEL, the importance of clinical application of anti-D-Ig was pointed out, and its antibody-mediated immunosuppressive mechanism was analyzed, which mainly includes red blood cell clearance, epitope blocking/steric hindrance, and Fc γ R Ⅱ B receptor mediated B cell inhibition, anti-D-Ig glycosylation, etc.; clarify the testing strategies of RhD blood group that should be adopted in response to the negative initial screening of pregnant and postpartum women; this article elaborates on the necessity of using anti-D-Ig in RhD-negative mothers after miscarriage or miscarriage, as well as the limitations of its application both domestically and internationally. It also proposes a solution strategy for detecting RhD blood group incompatibility HDFN as early as possible, diagnosing it in a timely manner, and using anti-D-Ig for its prevention and treatment. If the DEL gene is defined as an Asian-type DEL, anti-D-Ig prophylaxis in women would be unnecessary. Finally, based on the specificity of RhD-negative individuals, the article looks forward to the application trend of anti-D-Ig in China. It also called for related drugs to be listed in China as soon as possible and included in medical insurance.</p></div>","PeriodicalId":23262,"journal":{"name":"Transfusion Clinique et Biologique","volume":"31 1","pages":"Pages 41-47"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allelic frequency variation of ACKR1 in three Algerian populations: Zenata, Reguibat, and Oran ACKR1在三个阿尔及利亚群体中的等位基因频率变化：Zenata、Reguibat和Oran。

IF 1.7 4区医学

Transfusion Clinique et Biologique Pub Date : 2024-02-01 DOI: 10.1016/j.tracli.2023.10.003

Leila Adda Neggaz , Tahria Deba , Asmahan Bekada , Djabaria Naima Meroufel Sebaa , Sounnia Mediene Benchekor , Soraya Benhamamouch

{"title":"Allelic frequency variation of ACKR1 in three Algerian populations: Zenata, Reguibat, and Oran","authors":"Leila Adda Neggaz , Tahria Deba , Asmahan Bekada , Djabaria Naima Meroufel Sebaa , Sounnia Mediene Benchekor , Soraya Benhamamouch","doi":"10.1016/j.tracli.2023.10.003","DOIUrl":"10.1016/j.tracli.2023.10.003","url":null,"abstract":"<div><h3>Introduction</h3><p>The discovery of the Duffy antigen is of great significance, given its essential role in immune response and various physiological processes. Genetic mutations in the Duffy gene not only affect antigen expression but also result in different antigen types. This underscores the importance of genetic characterization for clinical studies and exploring genetic diversity within the population. This study primarily aims to genetically characterize the Duffy blood group within three Algerian populations: the Zenata, Reguibat, and Oran populations.</p></div><div><h3>Methods</h3><p>The genetic polymorphism of the Duffy erythrocyte group was examined, focusing on five allelic versions of the ACKR1 locus: <em>FY*01</em>, <em>FY*02</em>, <em>FY*X</em>, and silent alleles <em>FY*01 N.01</em> and <em>FY*02 N.01</em>. A total of 223 Algerian individuals, including 90 from the Oran population, 66 from the Zenata population, and 67 from the Reguibat population, were analyzed using the polymerase chain reaction with sequence-specific primer (PCR-SSP) method.</p><p>The results revealed the presence of the silent alleles (<em>FY*01 N.01 and FY*02 N.01</em>) in all three populations, with a total frequency of 78.03% in the Zenata population. Additionally, the <em>FY*X</em> allele was exclusively detected in the Reguibat population, with a frequency of 0.75%</p></div><div><h3>Conclusion</h3><p>This study provides valuable insights into the allele and genotypic frequencies of the Duffy system in the Zenata, Reguibat and Oranpopulations, contributing to our understanding of the genetic history and origins of the Algerian population. Further research incorporating additional genetic markers and establishing a comprehensive database would enhance our knowledge in this area.</p></div>","PeriodicalId":23262,"journal":{"name":"Transfusion Clinique et Biologique","volume":"31 1","pages":"Pages 7-12"},"PeriodicalIF":1.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49686964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0