Translational Stroke Research最新文献

{"title":"Serum Neurofilament Light Chain Predicts Stroke Outcome and is a Potential Marker for Treatment Effects of Neural Stem Cell-derived Extracellular Vesicles in a Rat Stroke Model.","authors":"Megan K Cannon, Alinde R Fojtik, Charles M White, Raymond L Swetenburg, Steven L Stice, Sean I Savitz, Emily W Baker","doi":"10.1007/s12975-026-01445-6","DOIUrl":"https://doi.org/10.1007/s12975-026-01445-6","url":null,"abstract":"<p><p>Acute ischemic stroke (AIS) remains a leading cause of disability worldwide, and effective treatments are urgently needed beyond reperfusion therapy. Translating preclinical success to clinical impact has been hindered by variability in animal models and the lack of translational biomarkers that predict outcomes across species. To overcome these barriers, we developed a robust rat AIS model optimized for consistency and severity, enabling rigorous therapeutic testing. Additionally, we tested a panel of common clinical serum biomarkers to improve translation from rodents to humans. We demonstrated that serum neurofilament light chain (NfL) -a biomarker widely used in clinical stroke studies- strongly correlated with functional outcomes, establishing a translational link that has not been previously reported in rats. Notably, NfL's predictive capabilities performed as well as infarct volume while outperforming serum biomarkers intercellular adhesion molecule-1 (ICAM-1) and S100 calcium binding protein (S100B). Using this platform, we evaluated the therapeutic impact of neural stem cell-derived extracellular vesicles (NSC EV), a novel biologic therapy poised for clinical trials, on stroke outcome in our rat AIS model. A three-dose regimen of NSC EV over 48 h confirmed our previous studies and produced the best outcomes in stroked animals evidenced by smaller infarct volume, improved neurologic score, and reduced serum NfL. In addition, single-dose and two-dose regimens of NSC EV were both effective at some endpoints. These findings not only validate NfL as a cross-species biomarker but also provide critical dosing insights for NSC EV therapy, accelerating the path from bench to bedside for AIS treatment.</p>","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic Analysis of Differentially Expressed Proteins in Ischemic Stroke Recovery and Their Implications for Therapeutic Outcomes.","authors":"Saquib Waheed, Sudong Qi, Hao Sun, Haiyang Shi, Xiangyu Hou, Shudong Liu, Changlong Zhou, Libo Zhao, Yuanlin Ma, Ke Cheng","doi":"10.1007/s12975-026-01441-w","DOIUrl":"https://doi.org/10.1007/s12975-026-01441-w","url":null,"abstract":"<p><p>Ischemic stroke is a condition characterized by the obstruction of blood flow to the brain, typically caused by a blood clot, leading to brain tissue damage and impaired neurological function. This study aims to use proteomic analysis to reveal the protein expression differences between different recovery results (meaningful recanalization [MFR] and futile recanalization [FTR]) in the recovery process of patients with ischemic stroke after thrombosis. We collected plasma samples from the healthy control (CON), MFR, and FTR groups, and used high-throughput data-independent collection (DIA) mass spectrometry for proteomic analysis. A total of 5,040 proteins were identified in the study, of which 775 were differentially expressed proteins (DEPs). The functional enrichment analysis revealed that these proteins are involved in lipid metabolism, amino acid synthesis, cell signaling regulation, and other pathways, especially between the FTR and MFR groups; the expression differences of specific proteins reflect biological differences in the recovery process. We identified 11 key DEPs, including Phospholipid Transfer Protein, Fructose-Bisphosphate Aldolase A, Alpha-Enolase 1, and Fatty Acid-Binding Protein, which may be potential biomarkers for predicting stroke recovery. This study provides new insights into the molecular mechanisms underlying recovery after ischemic stroke, particularly the molecular differences associated with distinct recovery outcomes, and identifies potential markers for personalized treatment and prognostic evaluation.</p>","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal Protein Biomarkers for Predicting Early Neurological Deterioration Following Intravenous Thrombolysis in Acute Ischemic Stroke.","authors":"Jiamin Li, Zixin Wang, Yingnan Zhou, Xinyi Wang, Qi Kong, Jiameng Li, Boyi Yuan, Yun Chen, Wan Wang, Jiapeng Zhao, Jinming Sheng, Haiping Zhao, Qingfeng Ma","doi":"10.1007/s12975-026-01444-7","DOIUrl":"https://doi.org/10.1007/s12975-026-01444-7","url":null,"abstract":"<p><p>Early neurological deterioration due to ischemic progression (ENDi) remains a poorly understood complication in acute ischemic stroke (AIS). We aim to uncover exosomal protein signatures for ENDi risk prediction and potential targets for intervention. From 110 prospectively enrolled AIS patients undergoing thrombolysis, we selected 3 ENDi and 5 age- and sex-matched early neurological improvement (ENI) patients. Plasma exosomes isolated before and 24 h post-thrombolysis were subjected to 4D-DIA proteomics, followed by Gene Set Enrichment Analysis (GSEA) and cellular origin analyses. Baseline differentially expressed proteins (DEPs) were further analyzed via functional enrichment and protein-protein interaction networks to identify candidate biomarkers and validated by ELISA in an independent cohort. We identified 1,238 exosomal proteins, among which 69 DEPs at baseline distinguished the ENDi from ENI patients. Cellular origin analysis revealed a post-thrombolytic reduction in monocyte-derived exosomal proteins in the ENDi group, while the ENI group showed upregulation of naive B cell-derived exosomal proteins. Regarding biological processes, ENDi after thrombolysis was characterized by activation of the coagulation and neuroinflammation pathways, along with suppression of the complement pathway and humoral immunity. From thrombo-inflammatory-related DEPs, we identified C-C motif chemokine 5 (CCL5) as a promising biomarker, and independent validation confirmed that elevated baseline plasma exosomal CCL5 was correlated with ENDi. In conclusion, our study highlights the role of exosomal proteins in immunothrombosis and establishes CCL5 as a potential predictor for ENDi. Importantly, we propose a novel mechanism involving immunoglobulin- and complement-related humoral immune suppression in the pathogenesis of ENDi, offering new directions for therapeutic intervention.</p>","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Intravenous Thrombolysis with Tenecteplase in Patients with Wake-Up Branch Atheromatous Disease.","authors":"Lili Zhu, Ying Zhang, Jiarong Li, Liang Song, Haoran Li, Yang Yang, Lijuan Liu, Shaolei Zhang, Xiaofei Gao, Weifeng Chen, Guo Rui, Haiqiang Qin, Mingbo Tian, Shengqi Fu","doi":"10.1007/s12975-026-01443-8","DOIUrl":"https://doi.org/10.1007/s12975-026-01443-8","url":null,"abstract":"<p><p>Branch atheromatous disease (BAD), a subtype of acute ischemic stroke (AIS), is associated with a high risk of early neurological deterioration (END) and poor prognosis. Wake-up stroke (WUS), comprising 20%-30% of AIS cases, is typically excluded from thrombolysis because of unknown onset time. Using diffusion-weighted imaging/fluid-attenuated inversion recovery (DWI/FLAIR) mismatch, we evaluated the efficacy and safety of Tenecteplase (TNK) thrombolysis in patients with BAD-related WUS. We retrospectively recruited 1,062 patients from seven Zhengzhou hospitals between January 2021 and June 2025. The patients received either TNK (n = 338) or dual antiplatelet therapy (n = 724). Propensity score matching (PSM; 1:1, caliper 0.02) yielded 292 matched pairs. Early neurological changes were evaluated using the National Institutes of Health Stroke Scale (NIHSS), and 90-day outcomes were evaluated using the modified Rankin Scale (mRS). After PSM, TNK significantly reduced the risk of END (odds ratio [OR] = 0.425, 95% confidence interval [CI]: 0.262-0.689; P < 0.001). Patients treated with TNK were more likely to achieve good functional outcomes (modified Rankin Scale [mRS] 0-1 and 0-2) with fewer poor outcomes (mRS ≥ 4). There were no significant differences in symptomatic intracranial hemorrhage, other bleeding events, or mortality among the groups. DWI/FLAIR mismatch-guided TNK thrombolysis appears to overcome the limitations of an unknown onset time in WUS and may counteract the progressive pathophysiology of BAD. TNK intravenous thrombolysis may be a safe and effective treatment for patients with wake-up BAD and DWI/FLAIR mismatches.</p>","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13132950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations Between Early Functional Brain Network Topology and 6-Month Upper Limb Motor Outcomes in Stroke Patients.","authors":"Ran Li, Yong Wang, Haimei Li, Binbin Nie, Jie Liu, Sujuan Liu","doi":"10.1007/s12975-026-01434-9","DOIUrl":"https://doi.org/10.1007/s12975-026-01434-9","url":null,"abstract":"","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147781984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing Infiltrating Macrophages in Intracranial Aneurysm with IA Animal Models and Spatial Transcriptomics.","authors":"Hosu Kim, Tomohiro Aoki, Masahiko Itani, Isao Ono, Yong-Hee Kim, Chung-Gyu Park","doi":"10.1007/s12975-026-01437-6","DOIUrl":"10.1007/s12975-026-01437-6","url":null,"abstract":"","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147781981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal Risk Stratification Clue of Carotid Bifurcation Plaques: A Cross-Sectional Observational Study of Vector Flow Imaging, Plaque-RADS, and Clinical Factors.","authors":"Liuming Shi, Yijie Ning, Qian Wang, Yalan Fang, Tian Yao, Jie Zhang, Kefangyuan Zheng, Sheng Yan, Haifeng Li, Yongbin Shi, Ruijing Zhang, Guiming Wang, Honglin Dong","doi":"10.1007/s12975-026-01440-x","DOIUrl":"https://doi.org/10.1007/s12975-026-01440-x","url":null,"abstract":"<p><p>This single-center cross-sectional study quantified segmental hemodynamic parameters of carotid bifurcation plaques by integrating high-frame-rate Vector Flow (V-Flow) imaging, Plaque-Reporting and Data System (Plaque-RADS) grading and clinical factors, to identify determinants of symptomatic status and enhance risk stratification of carotid atherosclerosis. From Feb-Aug 2025, 160 consecutive patients with carotid bifurcation plaques were enrolled. Symptomatic patients had ipsilateral transient ischemic attack/ischemic stroke within 180 days. B-mode ultrasound and V-Flow quantified wall shear stress (WSS), oscillatory shear index (OSI), and time-averaged turbulence intensity (TATur) at proximal/middle/distal plaque segments; plaques were Plaque-RADS-graded. Associations with symptomatic status were analyzed via multivariable logistic regression. Model performance was evaluated using ROC curve analysis and decision curve analysis; interobserver reproducibility was assessed using Bland-Altman analysis. Compared with the asymptomatic group patients (n = 98), symptomatic patients (n = 62) were older and had higher prevalence of hypertension, coronary heart disease, elevated triglycerides, and greater plaque length/thickness. They exhibited more Plaque-RADS grades 3-4, higher OSI/TATur at middle segments, and increased WSSmax/WSSmean, OSI, and TATur at distal segments. Multivariable analysis identified triglycerides, plaque length, TATur-Mid, WSSmax-Distal, and Plaque-RADS as independent predictors. The combined model (clinical + Plaque-RADS + V-Flow) achieved the highest discrimination (AUC = 0.826) and outperformed the base model. V-Flow-derived hemodynamic metrics, particularly OSI and TATur in middle/distal plaque segments, are strongly associated with symptomatic carotid disease. Combined with Plaque-RADS and clinical factors, these parameters enhance prediction of high-risk plaques, support individualized risk stratification, and may help identify patients who could benefit from closer surveillance.</p>","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative EEG in post-stroke delirium: Spectral and Functional Connectivity Analyses in a Case-Control Study.","authors":"Fenne Vandervorst, Edwin van Dellen, Robin Gens, Yacine Boudiba, Anissa Ourtani, Yorben Lodema, Guy Nagels, Anne-Marie Vanbinst, Eduard Druyts, Wilfried Cools, Sebastiaan Engelborghs, Arjen J Slooter, Sylvie De Raedt","doi":"10.1007/s12975-026-01413-0","DOIUrl":"https://doi.org/10.1007/s12975-026-01413-0","url":null,"abstract":"","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Tryptophan Hydroxylase 1 Activity-related Metabolic Markers and Stroke Risk: A Nested Case-control Study.","authors":"Yingjun Mu, Ruirui Ma, Qingyao Wang, Xudong Wang, Shujun Gu, Hui Zuo","doi":"10.1007/s12975-026-01439-4","DOIUrl":"https://doi.org/10.1007/s12975-026-01439-4","url":null,"abstract":"","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal Monitoring Results Around Delayed Cerebral Infarction After Aneurysmal Subarachnoid Hemorrhage.","authors":"Michael Veldeman, Stefan Yu Bögli, Ihsane Olakorede, Catharina Conzen-Dilger, Miriam Weiss, Nick Kastenholz, Jan Killermann, Erta Beqiri, Katharina Seyfried, Charlotte S Weyland, Hans Clusmann, Gerrit Alexander Schubert, Anke Hoellig, Peter Smielewski","doi":"10.1007/s12975-026-01435-8","DOIUrl":"https://doi.org/10.1007/s12975-026-01435-8","url":null,"abstract":"","PeriodicalId":23237,"journal":{"name":"Translational Stroke Research","volume":"17 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}