Translational Research最新文献

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tiRNA-HAR contributes to ischemic myocardial injury via facilitating HuR-mediated stability of p53 tiRNA-HAR通过促进hr介导的p53稳定性参与缺血性心肌损伤
IF 6.4 2区 医学
Translational Research Pub Date : 2025-04-17 DOI: 10.1016/j.trsl.2025.04.002
Changhao Wang , Yuelin Gao , Kuiwu Liu , Yaozhi Zhang , Zhiyong Sun , Min Huang , Zhezhe Qu , Shuting Yu , Jiaqi Han , Zhongting Mei , Shunkang Dou , Jianhao Jiang , Ying Li , Na Li , Chuanhao Huang , Yuechao Dong , Baofeng Yang , Weijie Du
{"title":"tiRNA-HAR contributes to ischemic myocardial injury via facilitating HuR-mediated stability of p53","authors":"Changhao Wang ,&nbsp;Yuelin Gao ,&nbsp;Kuiwu Liu ,&nbsp;Yaozhi Zhang ,&nbsp;Zhiyong Sun ,&nbsp;Min Huang ,&nbsp;Zhezhe Qu ,&nbsp;Shuting Yu ,&nbsp;Jiaqi Han ,&nbsp;Zhongting Mei ,&nbsp;Shunkang Dou ,&nbsp;Jianhao Jiang ,&nbsp;Ying Li ,&nbsp;Na Li ,&nbsp;Chuanhao Huang ,&nbsp;Yuechao Dong ,&nbsp;Baofeng Yang ,&nbsp;Weijie Du","doi":"10.1016/j.trsl.2025.04.002","DOIUrl":"10.1016/j.trsl.2025.04.002","url":null,"abstract":"<div><div>Cardiomyocyte death due to heart occlusion of coronary artery is the main driver to myocardial infarction (MI) and subsequent heart failure progression. tsRNA, a small RNA fragment from tRNA, has been shown to be implicated in many physiological and pathological processes by exerting different biological functions, but the roles of tsRNA in ischemic cardiac injury remain to be determined. The present study identified a hypoxia responsive-tiRNA (tiRNA-HAR) was markedly upregulated in ischemic mouse myocardium and hypoxic cardiomyocytes, respectively. Enforced expression of tiRNA-HAR by transfecting its mimic caused and aggravated, while knockdown of tiRNA-HAR mitigated cardiomyocyte apoptosis upon hypoxia. Cardiac specific knockdown of tiRNA-HAR mediated by AAV9 (adeno-associated virus 9) harboring an antisense oligonucleotide reduced cardiomyocytes apoptosis and improved cardiac function after MI. Mechanistically, tiRNA-HAR directly bound to HuR and enhanced the binding capacity of HuR and p53, thereby increasing the stability of p53. Silencing of HuR partially reversed the aggravative effects of tiRNA-HAR overexpression on cardiomyocyte apoptosis in the context of hypoxia. Collectively, our study reveals that tiRNA-HAR play a critical role in regulating cardiomyocytes apoptosis and cardiac injury via targeting HuR/p53 signaling axis after MI, and tiRNA-HAR might be a novel therapeutic target for treatment of ischemic heart disease.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"280 ","pages":"Pages 17-28"},"PeriodicalIF":6.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Loss of peptidylarginine deiminase 4 mitigates maladaptive cardiac remodeling after myocardial infarction through inhibition of inflammatory and profibrotic pathways 肽精氨酸脱亚胺酶4的缺失通过抑制炎症和纤维化途径减轻心肌梗死后不适应的心脏重构
IF 6.4 2区 医学
Translational Research Pub Date : 2025-04-17 DOI: 10.1016/j.trsl.2025.04.003
Michelle Holthaus , Xiaolin Xiong , Kaveh Eghbalzadeh , Clara Großmann , Simon Geißen , Fabian Piontek , Martin Mollenhauer , Ali T. Abdallah , Thomas Kamphausen , Markus Rothschild , Thorsten Wahlers , Adnana Paunel-Görgülü
{"title":"Loss of peptidylarginine deiminase 4 mitigates maladaptive cardiac remodeling after myocardial infarction through inhibition of inflammatory and profibrotic pathways","authors":"Michelle Holthaus ,&nbsp;Xiaolin Xiong ,&nbsp;Kaveh Eghbalzadeh ,&nbsp;Clara Großmann ,&nbsp;Simon Geißen ,&nbsp;Fabian Piontek ,&nbsp;Martin Mollenhauer ,&nbsp;Ali T. Abdallah ,&nbsp;Thomas Kamphausen ,&nbsp;Markus Rothschild ,&nbsp;Thorsten Wahlers ,&nbsp;Adnana Paunel-Görgülü","doi":"10.1016/j.trsl.2025.04.003","DOIUrl":"10.1016/j.trsl.2025.04.003","url":null,"abstract":"<div><div>Inflammation and progressive fibrosis represent predictive risk factors for heart failure (HF) development following myocardial infarction (MI). Peptidylargininine deiminase 4 (PAD4) catalyzes the citrullination of arginine residues in polypeptides and has recently been identified as a contributor to HF pathogenesis. This study aimed to evaluate the role of PAD4 in monocytes / macrophages (Mo/Mφ) and cardiac fibroblasts (CFs) for cardiac repair following MI and HF progression. Cardiac <em>Padi4</em> expression significantly increased in mice subjected to MI by permanent coronary artery ligation as well as in humans who died from MI. Transcriptome analysis revealed marked downregulation of inflammation-related genes in infarcted hearts and cardiac Mo/Mφ from global PAD4 knockout (PAD4<sup>−/−</sup>) mice on day 7 post-MI accompanied by increased frequency of reparative CD206<sup>+</sup> macrophages. Mechanistically, pharmacological and genetic PAD4 inhibition abrogated nuclear NF-κB translocation and inflammatory gene expression in bone marrow-derived macrophages (BMDM). Simultaneously, reduced inflammation and diminished cardiac levels of transforming growth factor-β (TGF-β) along with impaired IL-6 / TGF-β signaling in PAD4<sup>−/−</sup> CFs were associated with decreased expression of fibrotic genes, reduced collagen deposition, improved cardiac function, and enhanced 28-day survival in PAD4<sup>−/−</sup> mice. Strikingly, whereas pharmacological PAD inhibition in the acute phase after MI exacerbated cardiac damage, treatment starting on day 7 ameliorated cardiac remodeling and improved long-term survival in mice. Collectively, we here identified PAD4 as a critical regulator of inflammatory genes in Mo/Mφ and of profibrotic pathways in CFs. Thus, therapeutic approaches directed against PAD4 are promising interventions to alleviate adverse cardiac remodeling and subsequent HF development.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"280 ","pages":"Pages 1-16"},"PeriodicalIF":6.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Kv1.3 expression on CD4 (+) T cells promotes interleukin-17A-associated airway inflammation and airway remodeling in asthma Kv1.3在CD4 (+) T细胞上的表达可促进哮喘患者白细胞介素- 17a相关的气道炎症和气道重塑
IF 6.4 2区 医学
Translational Research Pub Date : 2025-04-10 DOI: 10.1016/j.trsl.2025.04.001
Bingqing Sun , Jiangtao Lin
{"title":"Kv1.3 expression on CD4 (+) T cells promotes interleukin-17A-associated airway inflammation and airway remodeling in asthma","authors":"Bingqing Sun ,&nbsp;Jiangtao Lin","doi":"10.1016/j.trsl.2025.04.001","DOIUrl":"10.1016/j.trsl.2025.04.001","url":null,"abstract":"<div><h3>Background</h3><div>Different types of T helper cells play an important role in disease severity and treatment response in patients with asthma. The potassium channel Kv1.3 is a type of potentially therapeutic target in T-cell-mediated inflammatory diseases.</div></div><div><h3>Objective</h3><div>This study aimed to explore the potential of Kv1.3 as a therapeutic target for asthma and to assess the efficacy of the Kv1.3 inhibitor PAP-1 in the treatment of asthma.</div></div><div><h3>Methods</h3><div>Kv1.3 expression on CD4<sup>+</sup>T cells was determined using data from public databases. CD4<sup>+</sup>T cells were isolated from peripheral blood samples obtained from healthy individuals and patients with asthma. The mouse models of OVA-induced asthma and Kv1.3 knockout were established. The underlying mechanism was investigated using mouse splenic CD4<sup>+</sup>T cells and BEAS-2B cells. OVA-induced asthmatic mice were treated with the Kv1.3 selective blocker PAP-1.</div></div><div><h3>Results</h3><div>Based on public data, we determined the distribution of Kv1.3 on CD4<sup>+</sup>T cells, its up-regulation in asthma, and its correlation with Th17/Treg balance. Upregulation of Kv1.3 in CD4<sup>+</sup>T cells was associated with enhanced activation of these cells and airway inflammation in patients and mice with asthma, accompanied by increased IL-17A levels in alveolar lavage fluid. Conversely, Kv1.3 deficiency significantly attenuated airway inflammation, lowered IL-17A levels in bronchoalveolar lavage fluid, and inhibited airway epithelial-mesenchymal transition in asthmatic mice. Furthermore, treatment with the Kv1.3 selective blocker PAP-1 attenuated inflammation in lung tissues and prevented airway remodeling in OVA-induced asthmatic mice.</div></div><div><h3>Conclusions</h3><div>Kv1.3 expression on CD4<sup>+</sup> T cells was correlated with IL-17A-associated airway inflammation and remodeling in asthma, which may be regarded as a potential diagnostic marker and therapeutic target for asthma.</div></div><div><h3>Translational significance</h3><div>Based on our study, Kv1.3 expression on CD4<sup>+</sup>T cells was correlated with IL-17A-associated airway inflammation and remodeling in asthma, which may be regarded as a potential diagnostic marker and therapeutic target for asthma. The treatment with the Kv1.3 selective blocker PAP-1 attenuated inflammation in lung tissues and prevented airway remodeling in OVA-induced asthmatic mice. Our discoveries offer novel perspectives for a better understanding of IL-17A-associated airway remodeling in asthma. The development of drugs targeting Kv1.3 holds application value for IL-17A-associated asthma.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"279 ","pages":"Pages 40-54"},"PeriodicalIF":6.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optical coherence tomography for the qualitative analysis of thyroid tissue images: Feasibility, features, and clinical potential 用于甲状腺组织图像定性分析的光学相干断层扫描:可行性、特征和临床潜力。
IF 6.4 2区 医学
Translational Research Pub Date : 2025-03-25 DOI: 10.1016/j.trsl.2025.03.003
Yun Shao , Qianru Xu , Cuixia Feng , Yang Liu , Baolei Jia , Yuning Song , Yuxuan Qiu , Qing Xu , Yanhong Tai , Feng Liang
{"title":"Optical coherence tomography for the qualitative analysis of thyroid tissue images: Feasibility, features, and clinical potential","authors":"Yun Shao ,&nbsp;Qianru Xu ,&nbsp;Cuixia Feng ,&nbsp;Yang Liu ,&nbsp;Baolei Jia ,&nbsp;Yuning Song ,&nbsp;Yuxuan Qiu ,&nbsp;Qing Xu ,&nbsp;Yanhong Tai ,&nbsp;Feng Liang","doi":"10.1016/j.trsl.2025.03.003","DOIUrl":"10.1016/j.trsl.2025.03.003","url":null,"abstract":"<div><div>The most important steps in thyroid surgery include distinguishing benign from malignant lesions and identifying the parathyroid glands. Optical coherence tomography (OCT) provides technical support for intraoperative guidance owing to its real-time, three-dimensional imaging capability. Benign and malignant diagnoses can be confirmed with intraoperative frozen sections; however, current approaches are time-consuming and labor-intensive and do not allow comprehensive, nondestructive tissue assessments. This study aimed to explore the use of OCT for imaging the thyroid tissue by verifying its clinical feasibility and qualitatively analyzing the OCT imaging characteristics of pathological thyroid glands. A customized swept-source OCT (SS-OCT) system was used to collect OCT data corresponding to the pathologies of 61 freshly excised tissue blocks containing either benign or malignant lesions from 45 patients. The OCT images were highly consistent with the H&amp;E histological images, verifying the feasibility of OCT in producing suitable images for analysis. The OCT-derived characteristics of the thyroid tissue were as follows: normal thyroid follicles presented with regularly arranged honeycomb structures; follicular nodular disease (FND) was characterized by heterogeneous nodules composed of follicles of different sizes, with multiple nodules also differing in size and consisting of varied reticular structures and a focally solid appearance; lymphocyte aggregation led to a gray‒black appearance in Hashimoto's thyroiditis tumors; and papillary thyroid carcinoma lesions were characterized by a heterogeneous texture and a low penetration depth. These results demonstrate the imaging capabilities of OCT for thyroid tissue with different pathological conditions and its broad prospects for clinical application.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"279 ","pages":"Pages 27-39"},"PeriodicalIF":6.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soluble ST2 is an early marker and treatment target for hypertensive nephrosclerosis signatured in glomerular mesangial cells 可溶性ST2是肾小球系膜细胞中高血压性肾硬化的早期标志物和治疗靶点。
IF 6.4 2区 医学
Translational Research Pub Date : 2025-03-15 DOI: 10.1016/j.trsl.2025.03.001
Jeong Min Cho , Seong Joon Park , Young Joo Kim , Saram Lee , Sunhwa Lee , Dha Woon Im , Semin Cho , Ran-hui Cha , Jae Wook Lee , Dong Ki Kim , Kook-Hwan Oh , Kwon Wook Joo , Yon Su Kim , Yong Chul Kim , Seung Hee Yang
{"title":"Soluble ST2 is an early marker and treatment target for hypertensive nephrosclerosis signatured in glomerular mesangial cells","authors":"Jeong Min Cho ,&nbsp;Seong Joon Park ,&nbsp;Young Joo Kim ,&nbsp;Saram Lee ,&nbsp;Sunhwa Lee ,&nbsp;Dha Woon Im ,&nbsp;Semin Cho ,&nbsp;Ran-hui Cha ,&nbsp;Jae Wook Lee ,&nbsp;Dong Ki Kim ,&nbsp;Kook-Hwan Oh ,&nbsp;Kwon Wook Joo ,&nbsp;Yon Su Kim ,&nbsp;Yong Chul Kim ,&nbsp;Seung Hee Yang","doi":"10.1016/j.trsl.2025.03.001","DOIUrl":"10.1016/j.trsl.2025.03.001","url":null,"abstract":"<div><div>The absence of a biologic marker for hypertensive nephrosclerosis (HN) remains a challenge. This study aimed at exploring the relationship between sST2 and early HN and examining their interaction in glomerular mesangial cells. The serum sST2 levels of healthy controls (HC; n=9), patients with HN (n=15), and patients with lupus nephritis (LN; n=27) were measured using ELISA. The association between the serum sST2 levels and clinicopathologic characteristics, including kidney function and mesangial proliferation, were assessed. The expression of ST2 and fibrotic markers in glomerular mesangial cells in hypertensive conditions was evaluated using a 5/6 nephrectomy rat model. To mimic intraglomerular hypertension, human primary glomerular mesangial cells (hPGMCs) were subjected to a 3-mmHg pressure using a newly developed mechanical pressurizing device. The cells were then treated with anti-ST2 antibody (0.5 and 1 μg/mL) to examine inflammation, apoptosis, and necrosis. The serum sST2 levels were significantly higher in the HN and LN groups than in the HC group. Increased expression of ST2 and fibrotic markers in glomerular mesangial cells was observed in the hypertension-induced animal model. In the <em>in vitro</em> study, pressure-stimulated hPGMCs exhibited increased population of early and late apoptosis, which were markedly reduced after treatment with anti-ST2 antibody (1.0 μg/mL). ST2 indicates the early pathologic changes of hypertensive kidney damage and may serve as a mesangial cell-specific marker for HN in terms of determining kidney function and pathologic findings. Thus, ST2 blockade could be a novel therapeutic approach for HN.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"279 ","pages":"Pages 16-26"},"PeriodicalIF":6.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of DNMT3a expression and nuclear translocation under ELAVL1 mediation for dendritic cell function and Th17/Treg balance in COPD ELAVL1调控下的DNMT3a表达和核转运对慢性阻塞性肺病树突状细胞功能和Th17/Treg平衡的作用
IF 6.4 2区 医学
Translational Research Pub Date : 2025-03-12 DOI: 10.1016/j.trsl.2025.03.002
Dan Huang , Bin Tang , Qiugen Li , Bo Tong , Na Liu
{"title":"Role of DNMT3a expression and nuclear translocation under ELAVL1 mediation for dendritic cell function and Th17/Treg balance in COPD","authors":"Dan Huang ,&nbsp;Bin Tang ,&nbsp;Qiugen Li ,&nbsp;Bo Tong ,&nbsp;Na Liu","doi":"10.1016/j.trsl.2025.03.002","DOIUrl":"10.1016/j.trsl.2025.03.002","url":null,"abstract":"<div><h3>Background</h3><div>Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. The DNA methyltransferase DNMT3a has been implicated in COPD, however its upstream regulation and downstream mechanisms remain unclear.</div></div><div><h3>Methods</h3><div>Relative mRNA and protein levels of indicated genes in lung tissues and dendritic cells (DCs) were tested via qRT-PCR and western blot, respectively. Cellular distribution of DNMT3a in DCs was determined by immunofluorescence staining. COPD mouse model was established by exposing mice to cigarette smoke (CS) via nose. The Th17/Treg cell ratio was examined using flow cytometry. Production of indicated cytokines was assessed by corresponding commercial ELISA kit. Interplay between DACH1 and c-Jun was verified by Co-immunoprecipitation, ChIP and luciferase reporter assays. Methylation level of DACH1 was tested by methylation specific PCR.</div></div><div><h3>Results</h3><div>DNMT3a expression was upregulated and negatively correlated with lung function in COPD patients. CS exposure increased pulmonary DNMT3a in mice. DNMT3a was predominantly expressed in the nucleus and CS exposure promoted its translocation to cytoplasm. RNA binding protein ELAVL1 upregulated DNMT3a expression, induced its nuclear translocation and increased its enzyme activity. DNMT3a promoted Th17 differentiation while inhibited Treg differentiation. DNMT3a methylated DACH1 and inhibited its expression, resulting in c-Jun pathway activation. In vivo DNMT3a knockdown ameliorated lung injury and Th17/Treg imbalance in COPD mice.</div></div><div><h3>Conclusion</h3><div>This study suggests that ELAVL1 regulates DNMT3a expression and nuclear translocation to modulate dendritic cell function and Th17/Treg balance through DACH1/c-Jun pathway in COPD.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"279 ","pages":"Pages 1-15"},"PeriodicalIF":6.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author Guidelines 作者指导方针
IF 6.4 2区 医学
Translational Research Pub Date : 2025-03-10 DOI: 10.1016/S1931-5244(25)00031-3
{"title":"Author Guidelines","authors":"","doi":"10.1016/S1931-5244(25)00031-3","DOIUrl":"10.1016/S1931-5244(25)00031-3","url":null,"abstract":"","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"278 ","pages":"Pages iii-iv"},"PeriodicalIF":6.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Information for Readers 读者资讯
IF 6.4 2区 医学
Translational Research Pub Date : 2025-03-10 DOI: 10.1016/S1931-5244(25)00032-5
{"title":"Information for Readers","authors":"","doi":"10.1016/S1931-5244(25)00032-5","DOIUrl":"10.1016/S1931-5244(25)00032-5","url":null,"abstract":"","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"278 ","pages":"Page IBC"},"PeriodicalIF":6.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial Advisory Board 编辑顾问委员会
IF 6.4 2区 医学
Translational Research Pub Date : 2025-03-10 DOI: 10.1016/S1931-5244(25)00030-1
{"title":"Editorial Advisory Board","authors":"","doi":"10.1016/S1931-5244(25)00030-1","DOIUrl":"10.1016/S1931-5244(25)00030-1","url":null,"abstract":"","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"278 ","pages":"Page ii"},"PeriodicalIF":6.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Caspase-12 exhibits non-redundant functions in response to endoplasmic reticulum stress to promote GSDMD-mediated NETosis, leading to thoracic aortic dissection Caspase-12在内质网应激反应中表现出非冗余功能,促进gsdmd介导的NETosis,导致胸主动脉夹层。
IF 6.4 2区 医学
Translational Research Pub Date : 2025-02-25 DOI: 10.1016/j.trsl.2025.02.005
Hanchuan Chen , Kun Yang , Shumin Zhang , Gulinazi Yesitayi , Yunzhi Ling , Rifeng Gao , Yang Lyu , Wei Wei , Jiaran Shi , Yulin Li , Xiang Ma , Pingjin Gao , Junbo Ge , Aijun Sun
{"title":"Caspase-12 exhibits non-redundant functions in response to endoplasmic reticulum stress to promote GSDMD-mediated NETosis, leading to thoracic aortic dissection","authors":"Hanchuan Chen ,&nbsp;Kun Yang ,&nbsp;Shumin Zhang ,&nbsp;Gulinazi Yesitayi ,&nbsp;Yunzhi Ling ,&nbsp;Rifeng Gao ,&nbsp;Yang Lyu ,&nbsp;Wei Wei ,&nbsp;Jiaran Shi ,&nbsp;Yulin Li ,&nbsp;Xiang Ma ,&nbsp;Pingjin Gao ,&nbsp;Junbo Ge ,&nbsp;Aijun Sun","doi":"10.1016/j.trsl.2025.02.005","DOIUrl":"10.1016/j.trsl.2025.02.005","url":null,"abstract":"<div><h3>Background</h3><div>Thoracic aortic dissection (TAD) is a highly lethal condition that is characterized by inflammatory cell infiltration. Recent evidence has indicated that Gasdermin D (GSDMD) plays an important role in vascular inflammation and degeneration. However, its effects on neutrophil extracellular trap formation and release (NETosis) during TAD remain unknown.</div></div><div><h3>Methods</h3><div>A TAD mouse model was generated using four-week-old male neutrophil-specific <em>GSDMD</em>-knockout mice (<em>GSDMD</em><sup>F/F</sup>; <em>Elane</em><sup>Cre</sup>) and dimethyl fumarate (DMF)-treated C57BL/6J mice by administering β-aminopropionitrile monofumarate (BAPN; 1 g/kg/day) in their drinking water for 4 weeks. Immunoprecipitation and immunofluorescence assays were performed to examine the role of the endoplasmic reticulum (ER) and its associated protein, caspase-12, in GSDMD-induced NETosis.</div></div><div><h3>Results</h3><div>GSDMD was elevated and co-localized primarily in neutrophils in the aortic tissues of patients with TAD and mice with BAPN-induced TAD. This was accompanied by increased NETosis. Neutrophil-specific <em>GSDMD</em> knockout and the NETosis inhibitor, GSK484, mitigated TAD development in mice. However, GSK484 did not provide additional therapeutic effects against TAD in the neutrophil-specific, <em>GSDMD</em> knockout mice. Mechanistically, ER stress promoted GSDMD cleavage by caspase-4/11, thereby inducing NETosis. Furthermore, caspase-12 exhibited non-redundant functions in the cleavage of GSDMD by caspase-4/11. The GSDMD inhibitor, DMF, partially prevented TAD development.</div></div><div><h3>Conclusions</h3><div>The ER stress/GSDMD/NETosis signaling pathway provides a potential therapeutic target for the prevention and treatment of TAD.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"278 ","pages":"Pages 48-60"},"PeriodicalIF":6.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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