Translational cancer research最新文献

{"title":"Ubiquitin-specific peptidase 20 affects immune cell infiltration by regulating prostaglandin E2 on intraperitoneal metastasis model of colorectal cancer.","authors":"Yang Hua, Xiukun Ma, Xinyu Zhao, Xiaomeng Wei, Xiaojing Mu, Botao Wang, Xiangfei Yuan","doi":"10.21037/tcr-2024-2194","DOIUrl":"10.21037/tcr-2024-2194","url":null,"abstract":"<p><strong>Background: </strong>Ubiquitin-specific peptidase 20 (USP20) acts as oncogene or tumor suppressor gene in different types of cancer. But there are only a few reports about the effect of USP20 on colorectal cancer (CRC). This study aims to further explore the novel molecular mechanisms of USP20 in intraperitoneal metastasis of CRC.</p><p><strong>Methods: </strong>First, bioinformatics analyses were performed to discover the association between USP20 expression and the clinical characteristics of CRC. Subsequently, the mouse model was constructed using a USP20 knockout (USP20-KO) colon cell line to validate the effects of USP20 on intraperitoneal metastasis and immune cell infiltration. And then metabolomics study was performed on the USP20-KO cells to find the novel mechanisms of USP20. Finally, the relationship of prostaglandin E2 (PGE2) and USP20 was identified by enzyme-linked immunosorbent assay (ELISA) and Western blotting experiments.</p><p><strong>Results: </strong>Bioinformatics analyses showed that the USP20 expression was significantly upregulated in CRC and affected immune cell infiltration. The animal model study revealed that the intraperitoneal metastasis of tumor cell was inhibited by knocking out USP20, and the expression of USP20 could affect the proportions of CD8⁺ T cells and myeloid-derived suppressor cells (MDSCs) in tumor tissue and ascites. Metabolomics study found that PGE2 should be the key metabolite regulated by USP20 for affecting immune cell infiltration. Finally, it was validated that the expressions of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) and microsomal prostaglandin E synthase-1 (mPGES-1) were regulated by the USP20 related ubiquitin-proteasome pathway.</p><p><strong>Conclusions: </strong>The USP20 expression is significantly upregulated in CRC, and can promote tumor metastasis by affecting immune cell infiltration. As an important tumor metabolite which can influence immune cell infiltration, PGE2 is regulated by USP20 through the protein degradation of mPGES-1 and 15-PGDH mediated by proteasome.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"3149-3160"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does circulating DNA tumor fraction add value to prostate-specific antigen in metastatic castration-resistant prostate cancer?","authors":"Cora N Sternberg, Maha Hussain","doi":"10.21037/tcr-2025-295","DOIUrl":"10.21037/tcr-2025-295","url":null,"abstract":"","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"2536-2538"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herpes simplex virus-thymidine kinase/ganciclovir suppressed the growth of lung adenocarcinoma cells accompanied by premature senescence.","authors":"Nan-Xi Yu, Zhi-Hui Li, Qing-Hua Yu, Xue Lu, Ling Gao","doi":"10.21037/tcr-24-1815","DOIUrl":"10.21037/tcr-24-1815","url":null,"abstract":"<p><strong>Background: </strong>Extensive laboratory research and clinical trial results have shown that herpes simplex virus-thymidine kinase/ganciclovir (HSV-TK/GCV) system has a therapeutic effect on various tumours. This study focused on the role of HSV-TK/GCV system therapy for lung adenocarcinoma.</p><p><strong>Methods: </strong>Cell proliferation, migration, invasion, and premature senescence were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony formation assay, wound-healing assay, invasion assay, and β-galactosidase staining, respectively. Cells were transfected with adeno-associated virus (AAV) vector expressing HSV-TK (AAV-TK) or green fluorescent protein (GFP) (AAV-GFP, control). A murine xenograft model of Lewis cells was established to investigate the effect of HSV-TK/GCV system on tumour growth. Protein expression related to cell proliferation and senescence in tumour was analysed by immunohistochemical staining.</p><p><strong>Results: </strong>AAV-TK transfection combined with GCV treatment significantly inhibited the proliferation, migration and invasion of A549 and Lewis cells compared with AAV-GFP transfection. β-galactosidase activity assay indicated that the premature senescence of cells was enhanced after AAV-TK/GCV treatment. <i>In-vivo</i> tumour growth was significantly inhibited after intratumour injection of AAV-TK/GCV. Immunohistochemical staining showed that the expression of p16 protein significantly increased while proliferating cell nuclear antigen (PCNA) expression decreased in tumour tissue after AAV-TK administration, which conformed that the proliferation of tumour cells was inhibited by AAV-TK/GCV treatment.</p><p><strong>Conclusions: </strong>HSV-TK/GCV system could significantly inhibit the growth and metastasis of lung adenocarcinoma, accompanied by cell premature senescence.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"2797-2807"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive assessment of disulfidptosis-related long non-coding RNA index as biomarkers for predicting clinical outcomes and immune microenvironment in pancreatic cancer.","authors":"Xiangyu Jin, Jinping Yang, Dongjing Li, Wendi Zhang, Qi Zhang, Mengxing Li, Yingquan Ye, Zhaohui Chen","doi":"10.21037/tcr-24-1976","DOIUrl":"10.21037/tcr-24-1976","url":null,"abstract":"<p><strong>Background: </strong>Disulfidptosis is a novel type of cell death that cannot be explained by the previous cell death approaches. Research on disulfidptosis may open the door to new therapeutic strategies for cancer. Long non-coding RNA (lncRNA) exerts a regulatory role in the cell death process. However, the potential value of disulfidptosis-associated lncRNAs in pancreatic adenocarcinoma (PAAD) has not yet been explored. Therefore, the aim of this study is to identify DRLncI related lncRNAs as a basis for establishing new predictive biomarkers in PAAD.</p><p><strong>Methods: </strong>The RNA-sequencing matrices of PAAD were extracted from The Cancer Genome Atlas (TCGA) cohort. Co-expression algorithm, Cox and the least absolute shrinkage and selection operator (LASSO) regression were conducted to determine a disulfidptosis-related lncRNA index (DRLncI). Kaplan-Meier method, Cox regression, and receiver operating characteristic algorithms were applied to assess the predictive stability and effectiveness of the DRLncI. Gene ontology, Gene Set Variation Analysis (GSVA) and tumour mutation burden analysis were employed for index-based mechanistic exploration. Additionally, the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE), single-sample Gene Set Enrichment Analysis (ssGSEA), Tumor Immune Estimation Resource (TIMER) platform and drug sensitivity were utilised to assess the predictive value of DRLncI for tumour immune microenvironment (TIME) and drug efficacy. In addition, consensus clustering algorithm was applied to distinguish PAAD subgroups with different molecular characteristics.</p><p><strong>Results: </strong>Based on disulfidptosis-related lncRNAs, we established a DRLncI consisting of seven lncRNAs. Multi-validation showed that DRLncI had better predictive stability and sensitivity than age and other clinical features. Additionally, DRLncI can well differentiate individuals with different TIME. Furthermore, DRLncI-based consensus clustering algorithm divided all individuals into two clusters. Systematic evaluation showed that the cluster 1 population not only had better prognosis, but also showed higher immune cell levels and immune checkpoints expression. Finally, DRLncI and consensus clustering analysis based on DRLncI can help determine the sensitivity of patients to different chemotherapeutic agents and targeted drugs, providing a reference for personalized treatment.</p><p><strong>Conclusions: </strong>The DRLncI and the DRLncI-based consensus clusters developed in the present research help to stratify the prognosis of individuals with PAAD, determine clinical outcomes and differentiate between patients with different TIME, providing a basis for personalized and precise oncology treatment.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"2758-2778"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning and deep learning to improve overall survival prediction in cervical cancer patients.","authors":"Nan Jiang, Xing Xiong, Xue Chen, Mengmeng Feng, Yan Guo, Chunhong Hu","doi":"10.21037/tcr-2024-2304","DOIUrl":"10.21037/tcr-2024-2304","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer (CC) is one of the most common gynecological malignancies. Previous studies have shown that the prognosis of CC is affected by many factors. Our study aimed to identify key prognostic factors and use machine learning and deep learning algorithms to construct models to predict the overall survival (OS) of CC patients.</p><p><strong>Methods: </strong>Data of CC patients collected between 2007 and 2016 were collected from the Surveillance, Epidemiology, and End Results (SEER) database, and were randomly divided into the training set (1,743 patients) and test set (747 patients). Moreover, in order to enhance the practical application of the model, we conducted an X-tile analysis to categorize the patients into three distinct strata based on their age and tumor size. Least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression were performed to identify the independent prognostic factors for OS, which were further used to construct CoxBoost, RandomForest, SuperPC XGBoost, and DeepSurv survival models to predict 1-, 3-, and 5-year OS.</p><p><strong>Results: </strong>The parameters, including age, marital status, grade, tumor size, surgery, radiation, race, the American Joint Committee on Cancer (AJCC)_stage, AJCC_T, and AJCC_M, were associated with survival and were further incorporated into the five models. The concordance index (C-index) value was 0.858, 0.848, 0.849, 0.840, and 0.869, respectively, and the receiver operating characteristic (ROC) curves showed exceptional predictive performance. Among the five models, DeepSurv was the model with best performance. The ROC curve validated the area under the curve (AUC) values for 1-year OS, 3-year OS, and 5-year OS, which were 0.936, 0.915, and 0.900, respectively.</p><p><strong>Conclusions: </strong>The prognostic model conducted by DeepSurv algorithm and the independent prognostic factors can potentially be applied in making personalized treatment plans and evaluating the prognosis of CC patients.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"3057-3068"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: H19/miR-152-3p/TCF4 axis increases chemosensitivity of gastric cancer cells through suppression of epithelial-mesenchymal transition.","authors":"Xiaodong Jiang, Wenbin Ding, Weiguang Shen, Jie Jin","doi":"10.21037/tcr-2025b-3","DOIUrl":"10.21037/tcr-2025b-3","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.21037/tcr-20-1736.].</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"3271"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: TGF-β signaling proteins and CYP24A1 may serve as surrogate markers for progesterone calcitriol treatment in ovarian and endometrial cancers of different histological types.","authors":"","doi":"10.21037/tcr-2025b-5","DOIUrl":"10.21037/tcr-2025b-5","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.21037/tcr.2019.07.36.].</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"3270"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The promise and translation of cell-free human papillomavirus DNA testing for cervical cancer surveillance.","authors":"Justin Garland, Kathleen N Moore, Joan L Walker, Doris M Benbrook","doi":"10.21037/tcr-2025-250","DOIUrl":"10.21037/tcr-2025-250","url":null,"abstract":"","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"2539-2543"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of periplocin on malignant behavior of oral squamous cell carcinoma cells.","authors":"Qian Zhao, Yueting Lu, Hualin Lu, Dixian Wang, Hongyue Shang, Manman Yao, Tiejun Liu","doi":"10.21037/tcr-24-2025","DOIUrl":"10.21037/tcr-24-2025","url":null,"abstract":"<p><strong>Background: </strong>Oral cancer ranks as the sixth most common malignancy worldwide, with a significantly higher prevalence among men aged 40 to 60 years than women. Previous studies have demonstrated that periplocin exhibits therapeutic potential by inhibiting cancer cell proliferation and inducing apoptosis across various cancer types. However, its role in oral squamous cell carcinoma (OSCC) and the associated molecular mechanisms remain insufficiently understood. In this study, we aimed to investigate the inhibitory effects of periplocin on the malignant behaviors of OSCC cells.</p><p><strong>Methods: </strong>MTS [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] assay, colony formation assay, flow cytometry, and transwell assay were employed to assess the effects of periplocin on OSCC cell proliferation, apoptosis, cell cycle progression, and migration. Furthermore, proteomics analysis was conducted to identify differentially expressed proteins in OSCC cells treated with periplocin, providing insights into its mechanisms of action.</p><p><strong>Results: </strong>Periplocin significantly influenced OSCC cell proliferation, apoptosis, cell cycle dynamics, and migration. Proteomics data indicated that periplocin modulates apoptosis, protein binding, and DNA replication signaling pathways. Furthermore, bioinformatics analysis revealed a strong association between syndecan 1 (<i>SDC1</i>) and coiled-coil-helix-coiled-coil-helix domain containing 2 (<i>CHCHD2</i>) in OSCC.</p><p><strong>Conclusions: </strong>The findings of this study indicate that periplocin exerts anti-OSCC effects by modulating key cellular processes in OSCC cells, offering a promising therapeutic avenue for OSCC management.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"2722-2736"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Limonin inhibits angiogenesis and metastasis of human breast cancer cells by suppressing the VEGFR2/IGFR1-mediated STAT3 signaling pathway.","authors":"","doi":"10.21037/tcr-2025b-2","DOIUrl":"10.21037/tcr-2025b-2","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.21037/tcr-20-1992.].</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"3264-3269"},"PeriodicalIF":1.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}