Translational cancer research最新文献

{"title":"The efficacy and safety of the addition of programmed cell death protein 1 inhibitor to preoperative chemotherapy in locoregionally advanced oropharyngeal carcinoma.","authors":"Guan-Zhong Lu, Zhi-Cong Hong, Yi-Feng Yu, Lin-Feng Guo, Daniel T Ginat, San-Gang Wu, Li-Mei Guan","doi":"10.21037/tcr-2025-202","DOIUrl":"10.21037/tcr-2025-202","url":null,"abstract":"<p><strong>Background: </strong>Immune checkpoint inhibitors have shown promise in improving the survival rates for recurrent and/or metastatic head and neck cancers. However, their impact on curative outcomes in head and neck cancers remains undefined, especially for those with locoregionally advanced oropharyngeal carcinoma (LAOPC), a subtype of head-and-neck malignancy closely associated with human papillomavirus infection. This study aimed to clarify the efficacy and safety of the addition of programmed cell death protein 1 (PD-1) inhibitor into preoperative chemotherapy in LAOPC.</p><p><strong>Methods: </strong>We retrospectively included patients with LAOPC who underwent preoperative immunochemotherapy between 2021 and 2024. Statistical analyses were conducted using chi-square tests. The efficacy was assessed using Response Evaluation Criteria in Solid Tumors (RECIST; version 1.1). Safety was evaluated using the Common Terminology Criteria for Adverse Events (CTCAE) 5.0.</p><p><strong>Results: </strong>A total of 23 patients were identified, and 11 (47.8%) had P16-positive tumors. There were 22 patients (95.7%) who completed two cycles of preoperative treatment. Among the 23 patients, the response to primary tumors and neck metastatic lymph nodes could be assessed in 21 and 22 patients, respectively. Additionally, 13 (61.9%) patients had a major pathologic response to the primary tumor, including 12 patients (57.1%) who achieved a pathologic complete response (PCR). In addition, 11 (50.0%) patients had a PCR in the metastatic cervical lymph nodes, while 11 (50.0%) patients still had residual tumors in the lymph nodes. The combined positive score and P16 status were not significantly associated with PCR to the primary tumor or neck metastatic lymph nodes. Moreover, 19 (82.6%) patients experienced treatment-related adverse effects, with the majority being grade 1-2 toxicities, and only 2 (8.7%) patients had grade 3 or higher toxicities. No treatment-related deaths occurred.</p><p><strong>Conclusions: </strong>The incorporation of a PD-1 inhibitor into preoperative chemotherapy may be an effective approach for treating LAOPC and involve acceptable toxicity.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"1401-1414"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel necroptosis-related miRNA signature for predicting the prognosis of esophageal cancer and immune infiltration analysis.","authors":"Miao Zhang, Shaoran Song, Bo Wang, Yangyang Shang, Peijun Liu, Juan Li","doi":"10.21037/tcr-24-1532","DOIUrl":"10.21037/tcr-24-1532","url":null,"abstract":"<p><strong>Background: </strong>The prognostic value of necroptosis-related microRNAs (miRNAs), which are important in tumorigenesis and development, remains unclear. Therefore, we aimed to screen prognostic necroptosis-related miRNAs in esophageal cancer (EC).</p><p><strong>Methods: </strong>Nine necroptosis-related miRNA expression profiles and associated clinical data of EC patients were obtained from The Cancer Genome Atlas (TCGA) database. The relationships between necroptosis-related miRNAs and overall survival (OS) were determined via Cox regression model analysis. Target genes of the miRNAs were investigated in TargetScan, miRDB, and miRTarBase. The biological functions of these genes were evaluated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. For the most significant correlation between miR-425-5p expression and the survival of EC patients, the effect of miR-425-5p on necroptosis was explored in EC cells. The relationship between targeted gene expression and immune infiltration was also analyzed and validated.</p><p><strong>Results: </strong>Hsa-miR-425-5p, hsa-miR-500a-3p, hsa-miR-7-5p and hsa-miR-200a-5p were selected for the construction of a prognostic signature based on their correlation with the survival of EC patients. EC patients were divided into high- and low-risk groups according to the median value of the risk score. Patients in the high-risk group tended to have higher death rates than those in the low-risk group (P<0.05). The risk score was an independent prognostic indicator for the OS of EC patients [hazard ratio (HR) >1, P<0.05]. The prognostic model had good predictive efficiency. The genes targeted by necroptosis-related miRNAs were significantly enriched in apoptosis etc. The inhibition of miR-425-5p promoted necroptosis in EC cells by targeting branched chain amino acid transaminase 1 (<i>BCAT1</i>). The expression level of <i>BCAT1</i> was significantly correlated with immune infiltration.</p><p><strong>Conclusions: </strong>A necroptosis-related four-miRNA model was constructed successfully to predict the potential value of the four miRNAs in the prognosis of EC, which can be conducive to promoting the therapeutic effect on EC.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"949-965"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing prognostic accuracy in invasive breast cancer by combining contrast-enhanced ultrasound and clinical data: a multicenter retrospective study.","authors":"Shiyu Li, Yueming Li, Yongqi Fang, Zhiying Jin, Sisi Huang, Wei Wang, Kefah Mokbel, Yongjie Xu, Hua Yang, Zhili Wang","doi":"10.21037/tcr-2025-96","DOIUrl":"10.21037/tcr-2025-96","url":null,"abstract":"<p><strong>Background: </strong>Current predictive models for disease-free survival (DFS) in invasive breast cancer predominantly utilize clinical and pathological factors, with minimal incorporation of ultrasound (US) and contrast-enhanced ultrasound (CEUS) characteristics. This study aimed to establish a multimodal map integrating US, clinical features, and US data to enhance the prediction of DFS in invasive breast cancer.</p><p><strong>Methods: </strong>The study utilized three retrospective datasets obtained from three academic medical centers, covering the period from March 2014 to December 2022. Clinical data, gray scale US, and CEUS were assessed in 942 adult patients undergoing breast cancer resection. The training and internal test sets were supplied by The First Medical Center of the PLA General Hospital, while the external test sets were sourced from The Fourth Medical Center of the PLA General Hospital and the Specialist Medical Center of the Strategic Support Forces. The patients were followed up by phone or clinic visits. DFS was evaluated as a prognostic outcome. Cox regression analysis identified prognostic factors, leading to the construction of three nomograms. The model performance was evaluated using the C-index, time-dependent receiver operating characteristic (ROC) curve, calibration, decision curve analysis (DCA), integrated discrimination improvement (IDI), and net reclassification index (NRI).</p><p><strong>Results: </strong>A total of 942 patients were enrolled, with a mean age of 51.91 years [interquartile range (IQR), 44.25-58.69 years]. The patients were included with the median DFS of 36 months. Cox regression analysis identified menopausal status, body mass index (BMI), color Doppler flow imaging (CDFI), tumor size on CEUS, adjuvant/neoadjuvant chemotherapy, progesterone receptor (PR) status, and tumor-node-metastasis (TNM) staging as significant risk factors for invasive breast cancer. The nomogram combining US, CEUS, and clinical data demonstrated excellent predictive performance, achieving a C-index of 0.811 in the training set, 0.816 in the internal validation set, and 0.819 in the external validation set. Calibration curves confirmed that the predicted survival probabilities aligned closely with observed outcomes. Comparative analysis of ROC curves, IDI, NRI, and DCA confirmed that the integrated nomogram outperformed models based solely on US and clinical data or clinical data alone in predicting 24- and 36-month DFS.</p><p><strong>Conclusions: </strong>The integration of CEUS and clinical factors for non-invasive DFS prediction improves personalized risk stratification, minimizing unnecessary interventions for low-risk patients and ensuring adequate monitoring for high-risk individuals. Additional prospective validation is required to establish its clinical applicability and incorporation into standard oncology practice.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"1336-1358"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive bioinformatics analysis of co-mutation of <i>FLG2</i> and <i>TP53</i> reveals prognostic effect and influences on the immune infiltration in ovarian serous cystadenocarcinoma.","authors":"Meng Li, Dongmei Han, Hao Jin","doi":"10.21037/tcr-24-1596","DOIUrl":"10.21037/tcr-24-1596","url":null,"abstract":"<p><strong>Background: </strong>Ovarian cancer remains one of the most lethal gynecological malignancies, characterized by late-stage diagnosis and high rates of recurrence. The present study aims to explore the prognostic and immunological implications of <i>FLG2</i> and <i>TP53</i>, the two genes exhibiting a high mutation frequency across various cancer types, in the context of ovarian serous cystadenocarcinoma (OV).</p><p><strong>Methods: </strong>The study systematically analyzed and discussed the potential implications of co-mutation of <i>FLG2</i> and <i>TP53</i> on prognosis and immune response using a cohort of 585 ovarian cancer samples. The differentially expressed genes (DEGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed on 300 ovarian cancer samples with RNA sequencing (RNA-seq) data.</p><p><strong>Results: </strong>The co-mutation of <i>FLG2</i> and <i>TP53</i> was identified in the 585 ovarian cancer cohort, and the group with co-mutation exhibited improved outcomes in terms of overall survival (OS), progression-free survival (PFS), and disease-specific survival (DSS). Additionally, the co-mutation (<i>FLG2</i> ⁺/<i>TP53</i> ⁺) group demonstrated higher scores in tumor mutation burden (TMB) comparing to that of the other three groups. The score of microsatellite instability (MSI) in the co-mutant group was only higher than that of the co-wild-type (<i>FLG2</i> ^-/<i>TP53</i> ^-). A total of 327 DEGs were identified in both the co-mutation and non-co-mutation (NCM) groups using limma analysis in the subgroup of 300 patients with RNA-seq data. Subsequent KEGG analysis revealed that these DEGs were implicated in various biological processes, including thermogenesis, Parkinson's disease (PD), and oxidative phosphorylation signaling pathways. Additionally, the co-mutation group exhibited elevated levels of various immune cells. Furthermore, a nomogram with high predictive accuracy was developed by integrating co-mutation status with clinical characteristics.</p><p><strong>Conclusions: </strong>In the context of OV, the concurrent mutation of <i>FLG2</i> and <i>TP53</i> not only induces immune activation, but also helps identify a subset of patients with a more favorable prognosis.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"1282-1296"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a prognostic model and analysis of related mechanisms in breast cancer based on multiple datasets.","authors":"Xiaofeng Wan, Jianmin Zhan, Shuke Ye, Chuanrong Chen, Runsheng Li, Ming Shen","doi":"10.21037/tcr-24-838","DOIUrl":"10.21037/tcr-24-838","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Breast cancer (BC) is a common tumor among women and is a heterogeneous disease with many subtypes. Each subtype shows different clinical presentations, disease trajectories and prognoses, and different responses to neoadjuvant therapy; thus, a new and universal prognostic biomarker for BC patients is urgently needed. Our goal is to identify a novel prognostic molecular biomarker that can accurately predict the outcome of all BC subtypes and guide their clinical management.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Utilizing data from The Cancer Genome Atlas (TCGA), we analyzed differential gene expression and patient clinical data. Weighted gene coexpression network analysis (WGCNA), Cox univariate regression and least absolute shrinkage and selection operator (LASSO) analysis were used to construct a prognostic model; the differential expression of the core genes in this model was validated via real-time quantitative polymerase chain reaction (RT-qPCR), and the reliability of the predictive model was validated in both an internal cohort and a BC patient dataset from the Gene Expression Omnibus (GEO) database. Further studies, such as gene set variation analysis (GSVA) and gene set enrichment analysis (GSEA), were performed to investigate the enrichment of signaling pathways. The CIBERSORT algorithm was used to estimate immune infiltration and tumor mutation burden (TMB), and drug sensitivity analysis was performed to evaluate the treatment response.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 1,643 differentially expressed genes were identified. After WGCNA and Cox regression combined with LASSO analysis, 15 genes were identified by screening and used to establish a prognostic gene signature. Further analysis revealed that the epithelial-mesenchymal transition (EMT) pathway gene signature was enriched in these genes. Each patient was assigned a risk score, and according to the median risk score, patients were classified into a high-risk group or a low-risk group. The prognosis of the low-risk group was better than that of the high-risk group (P&lt;0.01), and analyses of two independent GEO validation cohorts yielded similar results. Furthermore, a nomogram was constructed and found to perform well in predicting prognosis. GSVA revealed that the EMT pathway, transforming growth factor β (TGF-β) signaling pathway and PI3K-Akt signaling pathway genes were enriched in the high-risk group, and the Wnt-β-catenin signaling pathway, DNA repair pathway and P53 pathway gene sets were enriched in the low-risk group. GSEA revealed genes related to TGF-β signaling and the PI3K-Akt signaling pathways were enriched in the high-risk group. CIBERSORT demonstrated that the low-risk group had greater infiltration of antitumor immune cells. The TMB and drug sensitivity results suggested that immunotherapy and chemotherapy are likely to be more effective in the low-risk group.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;We established a new EMT pathway-rel","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"930-948"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osimertinib for uncommon EGFR mutations: herding UNICORNs in a field of horses.","authors":"Sean C Dougherty, Ryan D Gentzler","doi":"10.21037/tcr-24-1936","DOIUrl":"10.21037/tcr-24-1936","url":null,"abstract":"","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"670-675"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare case report of colon metastasis from primary oropharyngeal squamous cell carcinoma treated with a combination of surgery, radiotherapy and immunotargeted therapy.","authors":"Zhijian Xu, Jianjian Lv, Daniel T Ginat, Yuvnik Trada, Mi Zhou","doi":"10.21037/tcr-24-1744","DOIUrl":"10.21037/tcr-24-1744","url":null,"abstract":"<p><strong>Background: </strong>Oropharyngeal squamous cell carcinoma (OPSCC) is a type of head and neck squamous cell carcinoma (HNSCC). The incidence of distant metastasis from HNSCC is approximately 10-15%. The most frequent organs of distant metastasis include the bone, liver, and lungs, and the occurrence of metastasis to the colon is very rare. To date, the treatment recommendation for these patients, especially local treatment, is unclear. This case reports a real case of a rare metastatic location from OPSCC, thus expanding our knowledge of OPSCC. At the same time, it suggests that local treatment has potential value in patients with metastatic HNSCC, especially for those with concomitant local symptoms.</p><p><strong>Case description: </strong>The patient, a 72-year-old male, underwent radical radiotherapy with concurrent chemotherapy for previously diagnosed with cT4N2M0 stage III HPV16 (+) (American Joint Committee on Cancer, 8th) OPSCC. The patient experienced diarrhea during the post-treatment monitoring period and subsequently underwent a re-examination. The positron emission tomography-computed tomography (PET-CT) findings confirmed colonic metastasis and axillary lymph node enlargement. After the surgical removal of the colon tumor, OPSCC metastasis was confirmed. PET-CT findings of other organs and primary lesions did not suggest recurrence or metastasis. This case provides a new reference for the pattern of disease progression in patients with HNSCC who have received radical concurrent radiochemotherapy. This patient achieved long-term, high-quality survival as a result of local surgery, radiotherapy, systemic immune checkpoint inhibitor therapy, and anti-vascular targeted therapy. The patient's progression-free survival (PFS) has reached 28 months, and apart from bone marrow suppression induced by chemotherapy, no adverse reactions have occurred during the combination of immunotherapy and targeted therapy.</p><p><strong>Conclusions: </strong>This case not only provides an example of a rare metastatic location of OPSCC, thus expanding our knowledge of OPSCC. At the same time, it suggests that local treatment has potential value in patients with metastatic HNSCC, especially for those with concomitant local symptoms.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"1492-1499"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prognostic and immune significance of <i>SNHG3</i> in clear cell renal cell carcinoma.","authors":"Cheng Li, Pengnan Hu, Chenglong Fan, Hua Mi","doi":"10.21037/tcr-24-1509","DOIUrl":"10.21037/tcr-24-1509","url":null,"abstract":"<p><strong>Background: </strong>Long non-coding RNA (lncRNA) small nucleolar RNA host gene 3 (<i>SNHG3</i>) has been reported to be involved in the pathological process of a variety of tumors, including clear cell renal cell carcinoma (ccRCC). However, whether <i>SNHG3</i> can be used as a prognostic biomarker and its correlation with immune infiltration in ccRCC remain unclear, warranting further research. This study aims to explore the relationship between <i>SNHG3</i> and immune infiltration in ccRCC and confirm the potential of <i>SNHG3</i> to predict survival of ccRCC patients.</p><p><strong>Methods: </strong>The Cancer Genome Atlas (TCGA) database was used to assess the expression of <i>SNHG3</i> in ccRCC, evaluate clinicopathological characteristics, assess prognosis, and conduct functional enrichment analysis. The ccRCC microenvironment and immune infiltration were investigated using the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) and Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) algorithms, respectively. We additionally investigated the relationships between <i>SNHG3</i> and immunological checkpoints. Drug sensitivity of <i>SNHG3</i> was investigated in R. The expression of <i>SNHG3</i> was verified in the Gene Expression Omnibus (GEO) database, ccRCC cell lines, and tissues. Wound healing and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assays were used to evaluate tumor cell migration and proliferation. Fluorescence in situ hybridization (FISH) assay was conducted to localize <i>SNHG3</i> in ccRCC cells.</p><p><strong>Results: </strong><i>SNHG3</i> expression was significantly upregulated in ccRCC cells and tissues and associated with several clinicopathological features and poor prognosis of ccRCC patients. <i>SNHG3</i> was correlated with immune cells infiltration in ccRCC and exhibited sensitivity to various targeted and chemotherapy drugs. Knockdown of <i>SNHG3</i> significantly reduced the proliferation and migration of ccRCC. FISH results showed that <i>SNHG3</i> was located in the cell nucleus.</p><p><strong>Conclusions: </strong>Overall, this study demonstrates that <i>SNHG3</i> is a prognostic biomarker correlated with immune infiltration in ccRCC.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"1008-1023"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FAM60A promotes proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin signaling pathway.","authors":"Zhikun Dong, Shuwen Jin, Kan Tang, Xiaomei Li, Yonglin Chen","doi":"10.21037/tcr-24-1608","DOIUrl":"10.21037/tcr-24-1608","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is one of the most detrimental tumors to human health. Although multimodal therapeutic approaches can improve patient survival rates, the prognosis for advanced-stage patients remains poor. It has been reported that family with sequence similarity 60, member A (FAM60A), a component of the SIN3 transcription regulator family member A (SIN3A)/histone deacetylase (HDAC) complex, plays a significant role in tumorigenesis. However, the precise function and mechanisms of action of FAM60A in CRC have not been fully elucidated. In this study, we aim to further delineate the role of FAM60A in CRC by assessing the protein expression levels of FAM60A and β-catenin in CRC tissues and to explore the potential mechanisms by which FAM60A may promote CRC cell proliferation and invasion through a suite of cellular assays.</p><p><strong>Methods: </strong>Tumor tissues of 195 CRC patients and 65 adjacent non-neoplastic tissues were collected to construct tissue microarrays. The expression levels of FAM60A, c-Myc, cyclin D1, and β-catenin were detected using immunohistochemistry (IHC) staining, and the relationship between the results and the patients' clinicopathological characteristics and prognosis was analyzed. HCT116 and HT-29 cell lines with overexpression/knockdown of FAM60A were constructed. Western blot (WB) was used to detect the protein expression of FAM60A and β-catenin. Cell proliferation, apoptosis rate, cell cycle, and cell migration and invasion abilities were assessed using cell counting kit-8 (CCK-8) assay, flow cytometry, wound healing assay, and transwell assay, respectively.</p><p><strong>Results: </strong>FAM60A demonstrated elevated expression in CRC tissues and was positively correlated with tumor infiltration depth, Ki67 proliferation index, and poor prognosis in patients. A positive correlation was observed between FAM60A and the expression of β-catenin, c-Myc, and cyclin D1, and patients with co-expression of FAM60A and β-catenin had a significantly higher rate of distant metastasis. The knockdown of FAM60A markedly reduced the proliferation, migration, and invasive capabilities of HCT116 cells, induced cell cycle arrest, and enhanced apoptosis, whereas its overexpression produced the converse effects. In HT-29 cells, FAM60A knockdown also reduced cell proliferation and impaired wound healing, with overexpression showing opposing outcomes. WB analysis revealed that modulation of FAM60A influenced β-catenin protein levels, suggesting a regulatory link between the two proteins.</p><p><strong>Conclusions: </strong>FAM60A may be a key regulator factor that modulates proliferation and invasion in CRC cells via the Wnt/β-catenin signaling pathway. Elevated FAM60A expression is associated with an adverse prognosis in CRC, underscoring its potential as a prognostic biomarker.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"1171-1189"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic accuracy of pleural fluid carbohydrate antigen 72-4 for malignant pleural effusion: a systematic review and meta-analysis.","authors":"Xi-Shan Cao, Chang-Mei Feng, Li Yan, Lei Zhang, Wei Jiao, Mei-Ying Wang, Wen-Qi Zheng, Zhi-De Hu","doi":"10.21037/tcr-24-1664","DOIUrl":"10.21037/tcr-24-1664","url":null,"abstract":"<p><strong>Background: </strong>Several studies have evaluated the diagnostic accuracy of pleural fluid carbohydrate antigen 72-4 (CA72-4) for malignant pleural effusion (MPE), but the results were diverse. This systematic review and meta-analysis aimed to evaluate the diagnostic accuracy of pleural fluid CA72-4 for MPE.</p><p><strong>Methods: </strong>The PubMed and Web of Science databases were searched to verify potential studies investigating the diagnostic accuracy of pleural fluid CA72-4 for MPE. The last search date was August 2024. The quality of the eligible studies was assessed by this study using the revised diagnostic accuracy study quality assessment tool-2 to assess the quality of the eligible studies. This study used a summary receiver operating characteristic (sROC) curve and a bivariate model to pool the findings and their 95% confidence intervals (CIs) of available studies.</p><p><strong>Results: </strong>Eight studies with 828 cases of MPEs and 963 cases of benign pleural effusion (BPE) were included in the present meta-analysis. The pooled sensitivity (95% CI) and specificity (95% CI) were 0.47 (0.39-0.55) and 0.98 (0.95-0.99). The area under sROC curves was 0.77 (95% CI: 0.73-0.80). The primary design weaknesses of the included studies were the representativeness of the participants and the data-driven threshold to define positive CA72-4. A significant publication bias was observed across the eligible studies.</p><p><strong>Conclusions: </strong>Pleural fluid CA72-4 is an auxiliary diagnostic marker for MPE. However, its diagnostic accuracy may be overestimated by available studies.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 2","pages":"1237-1245"},"PeriodicalIF":1.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}