Toxicology International最新文献_第2页

Formulation and Evaluation of Polymeric Nanoparticles as Carriers of Rosuvastatin Calcium for Oral Administration 作为瑞舒伐他汀钙口服载体的聚合物纳米颗粒的制备与评估

Toxicology International Pub Date : 2023-11-10 DOI: 10.18311/ti/2023/v30i4/33447

M. Thenmozhi, T. Suganya, Gokul Marimuthu

{"title":"Formulation and Evaluation of Polymeric Nanoparticles as Carriers of Rosuvastatin Calcium for Oral Administration","authors":"M. Thenmozhi, T. Suganya, Gokul Marimuthu","doi":"10.18311/ti/2023/v30i4/33447","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/33447","url":null,"abstract":"Aim of this research was to formulate and evaluate the polymeric nanoparticle as carriers of rosuvastatin calcium for oral administration. Rosuvastatin calcium-loaded nanoparticles were formulated by nanoprecipitation method using different ratios of polymers (Eudragit L100 and Eudragit S100) and different concentrations of stabilizers (Pluronic F68 and PVA) with constant drug concentration. The formulations were evaluated for particle size, zeta potential, drug content, entrapment efficiency, in vitro release, kinetics, solubility, ex vivo intestinal permeability and Transmission Electron Microscopy (TEM). Fourier Transform-Infrared (FT-IR) spectroscopy and Differential Scanning Calorimetry (DSC) studies were carried out to check compatibility between the drug and polymers. No significant drug-polymer interactions were found. To enhance drug entrapment particle size range from 100-250 nm were prepared and entrapment efficiencies were found be 28-79 %. In vitro release studies showed a biphasic release pattern of rosuvastatin calcium from nanosuspensions: One initial burst release in the first 2 hours which could be helpful to improve the penetration of drug followed by a second slow release phase consistent with a Higuchi diffusion mechanism. The solubility of rosuvastatin calcium loaded polymeric nanoparticles compared to pure drug form was increased to about two-fold. Intestinal permeability of rosuvastatin calcium entrapped in Eudragit L100 an Eudragit S100 nanoparticles across rat small intestinal segments was significantly improved compared with rosuvastatin calcium in solution. Nanoparticles observed by TEM showed extremely spherical shapes. Results indicated that nanoparticle formulations could be a promising delivery system for oral administration of rosuvastatin calcium with enhanced solubility, intestinal permeability and improved oral bioavailability.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"31 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139280243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Stability-Indicating HPLC Method for Analyzing Process Related Impurities of Penfluridol and Structural Elucidation of Stress Degradation Products by LCMS/MSucidation of Stress Degradation Products by LCMS/MS 优化用于分析五氟利多工艺相关杂质的稳定性指示高效液相色谱法，并通过 LCMS/MS 对应激降解产物进行结构阐释通过 LCMS/MS 对应激降解产物进行结构阐释

Toxicology International Pub Date : 2023-11-06 DOI: 10.18311/ti/2023/v30i4/34033

Beram Adilakshmi, V. K. Rohini, T. Eswarlal, Ch. Lakshmi Prasanna, Venkateswara Rao Anna

{"title":"Optimization of Stability-Indicating HPLC Method for Analyzing Process Related Impurities of Penfluridol and Structural Elucidation of Stress Degradation Products by LCMS/MSucidation of Stress Degradation Products by LCMS/MS","authors":"Beram Adilakshmi, V. K. Rohini, T. Eswarlal, Ch. Lakshmi Prasanna, Venkateswara Rao Anna","doi":"10.18311/ti/2023/v30i4/34033","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/34033","url":null,"abstract":"This study focused on the development of a simple and sensitive HPLC method for resolution and quantification of process-related impurities of penfluridol and further assessment of forced degradation behavior of penfluridol. The chromatographic separation was achieved on XTerra™ C18 (250×4.6 mm, 5.0μm) column and UV detection at 245nm. The mobile phase comprises of methanol and tetrahydrofuran in 55:45 (v/v) as solvent A and acetonitrile and tetrahydrofuran in 80:20 (v/v) as solvent B. The 60:40 (v/v) composition of solvent A and B were pumped isocratically at 1.0mL/min. In the proposed conditions, the retention time identified as 5.29 min for penfluridol, 4.51 min, 9.95 min and 7.64 min respectively for impurity 1, 2 and 3 with acceptable system suitability. The method produces sensitive detection limit of 0.008μg/mL for impurity 1, 2 and 0.004 μg/mL for impurity 3 with calibration range of 25-150 μg/mL for penfluridol and 0.025-0.150 μg/mL for impurities. The drug was exposed to different stressed conditions (acid, base, peroxide, thermal and UV light) according to ICH Q1A (R2) guidelines. The Degradation Products (DPs) formed during the stress study was characterized by LCMS/MS in ESI positive mode and the possible structures of five DPs with possible degradation pathways were proposed. The outcomes of other validation studies were likewise satisfactory and proven adequate for regular analysis of penfluridol and its process-related impurities in bulk drug and pharmaceutical dosage forms and can also applicable for evaluation of stress degradation mechanism of penfluridol.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review on the Impact of Pesticide Toxicity on the Physiological and Behavioral Condition of Fish 农药毒性对鱼类生理和行为状况的影响综述

Toxicology International Pub Date : 2023-11-03 DOI: 10.18311/ti/2023/v30i4/34317

Sayan Mandal, Basudev Mandal

{"title":"A Review on the Impact of Pesticide Toxicity on the Physiological and Behavioral Condition of Fish","authors":"Sayan Mandal, Basudev Mandal","doi":"10.18311/ti/2023/v30i4/34317","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/34317","url":null,"abstract":"Pesticides are an essential component of contemporary agriculture because they help keep unwanted insects and animals under control and boost crop yields. However, the widespread usage of pesticides has led to worries over the unexpected consequences that these chemicals have on non-target animals and ecosystems, particularly those that are aquatic. Fish, which play an essential role in aquatic food webs, are particularly vulnerable to the damaging effects of pesticides as a result of their direct contact with contaminated water bodies. The review covers acute and chronic pesticide effects on fish, including physiological and behavioral responses. It discusses population-level effects and biodiversity loss on fish reproduction, growth, development, immunological function, and locomotor activity. It also highlights pesticide toxicity’s long-term effects on fish populations. Pesticide exposure may also alter foraging behavior, competitive aptitude, and predation vulnerability, according to the review. Pesticides harm fish health by accumulating toxins, causing genetic defects, and upsetting the aquatic ecology. These effects put biodiversity in jeopardy and upset the food chain’s delicate balance, raising major environmental issues. It emphasizes the need for interdisciplinary research to better understand fish pesticide toxicity and guide environmental regulatory measures. Implementation of Integrated Pest Management (IPM) practices to ensure sustainable pesticide use in the environment. Use of non-chemical strategies such as crop rotation, natural predators, and resistant varieties. limit pesticide application to specific pests, thereby minimizing ecological damage and preserving ecosystem equilibrium. We can improve agriculture-aquatic biodiversity coexistence by supporting holistic pesticide management.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Role of Allicin in Arsenic-Induced Liver and Gonad Apoptosis in Male Swiss Albino Mice (Mus musculus) 大蒜素对砷诱导的雄性瑞士白化小鼠肝脏和性腺凋亡的改善作用

Toxicology International Pub Date : 2023-11-03 DOI: 10.18311/ti/2023/v30i4/34093

Payel Sarkar, Jayanta Kr. Kundu

{"title":"Ameliorative Role of Allicin in Arsenic-Induced Liver and Gonad Apoptosis in Male Swiss Albino Mice (Mus musculus)","authors":"Payel Sarkar, Jayanta Kr. Kundu","doi":"10.18311/ti/2023/v30i4/34093","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/34093","url":null,"abstract":"Arsenic toxicity is a matter of concern in the present-day world. Arsenic, a potentially hazardous metalloid easily gets biomagnified through the food chain and also affects not only the vital organs such as the kidney, and liver of human beings but also the reproductive organs of males. This study was designed to propose allicin, the main bio-active component of garlic to address arsenic toxicity more efficiently and without any side effects apart from the costly conventional chelation therapy which is not free from various side effects. To conduct this study, allicin has been quantified and collected from ethanolic extract of garlic by High-Performance Liquid Chromatography (HPLC) using C18 column at 254nm wave-lengths against standard allicin at the retention time of 18.775min. The calculated concentration of allicin is 77.80%. Next, arsenic trioxide (As2O3) (20mg/kg) and allicin (100mg/kg) were administered orally for toxicity and treatment respectively in mice (Mus musculus) for 30 days. Compared to the control group, liver marker enzymes i.e., Serum Glutamic Pyruvate Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT), and Alkaline Phosphatase (ALP) levels were significantly increased in the arsenic-induced group. On the other hand, co-treatment with allicin significantly recovered liver enzyme parameters to normal levels (p<0.05). Decreased weight of testis, sperm count, and increased numbers of Sperm Head Anomalies (SHA) indicate reduced reproductive potential in the arsenic-induced group of male albino mice. On the contrary, co-treatment with allicin significantly increased testis weight, sperm count and decreased SHA count (p<0.05). On examining histological slides of the liver and testis, normal histo-architecture was observed in both control and arsenic-induced allicin co-treated groups; whereas damage was observed in the arsenic-induced group. Generated data pointed out that allicin offers significant protection to the mammalian liver and male gonad (testis) against arsenicinduced toxicity.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Lead Toxicity on Wild Cannabis Species of Punjab Region 铅毒性对旁遮普地区野生大麻物种的影响

Toxicology International Pub Date : 2023-11-03 DOI: 10.18311/ti/2023/v30i4/31022

Dimpy Balgotra, Sabreen Bashir, Agrataben Vadhel, Madhuri Girdhar, Anand Mohan

{"title":"Effect of Lead Toxicity on Wild Cannabis Species of Punjab Region","authors":"Dimpy Balgotra, Sabreen Bashir, Agrataben Vadhel, Madhuri Girdhar, Anand Mohan","doi":"10.18311/ti/2023/v30i4/31022","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/31022","url":null,"abstract":"Human activities are causing environmental pollution in many ways by contaminating air, water and soil by adding different types of pollutants. Among various pollutants, heavy metals are an emerging threat in today’s world which are degrading our environment in a number of ways. Lead is the most widespread and evenly distributed poisonous element. Due to urbanization and growing human activities, lead emissions in different forms have increased, resulting in the contamination of soil and water. The lead transfers from environment to different forms of life, disrupting biological processes and causing various health issues. In this article, pot culture experiments were carried out to analyse the stress of Lead (Pb) and the capability of Cannabis sp. to tolerate the stress by studying the impact of different concentrations (0 mg/kg, 50 mg/kg, 100 mg/kg and 150 mg/kg of soil) on various biochemical aspects of the plant (Photosynthetic pigments, protein, antioxidant enzyme activity). Lead was given in the form of lead acetate. The results showed that the photosynthetic pigments-chlorophyll and carotenoid decreased with increasing lead concentration. Same effect was shown by the protein content in the leaves. On the other hand, Superoxide Dismutase (SOD), which is an antioxidant enzyme, increased with increasing concentration of lead.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical Animal Models of Renal Disease 肾病临床前动物模型

Toxicology International Pub Date : 2023-11-03 DOI: 10.18311/ti/2023/v30i4/34635

Kunal Bahalkar, Manish Musale, J. Kakadiya

{"title":"Preclinical Animal Models of Renal Disease","authors":"Kunal Bahalkar, Manish Musale, J. Kakadiya","doi":"10.18311/ti/2023/v30i4/34635","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/34635","url":null,"abstract":"Acute Renal Failure (ARF) is a serious condition where the kidneys suddenly stop working, commonly caused by drug-related injury. This article aims to give a detailed explanation of different animal models used to study ARF, focusing on the biomarkers linked with this condition. When administering drugs to animals, it is essential to be mindful of the potential for ARF to occur. Nephrotoxic drugs like cisplatin, methotrexate, acyclovir, Cyclosporine, folic acid, amphotericin B, and amikacin can induce ARF if the dosage and duration of exposure are not adequately regulated to match the clinical scenario. Careful monitoring is crucial to ensuring the safety and well-being of the animals under our care. This article contains various screening models for ARF caused by various allopathic drugs like glycerol, acyclovir, amikacin, amphotericin B, Isoniazid-Rifampicin, cisplatin, folic acid, diclofenac, and lithium. The intrinsic toxicity of these medications also plays a significant role in the ensuing Acute Kidney Injury (AKI), and the kidney is probably more vulnerable to damage than other organs. These medications can be hazardous and their effects on the glomerulus and/or tubules can be caused by oxidative damage, hypersensitivity responses, altered hemodynamics, and tubule blockage. This article aims to provide a thorough description of the model used and to examine the findings in relation to that particular model. This approach can yield valuable insights and help ensure the findings’ accuracy and relevance.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"185 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophylactic Efficacy of Allium sativum Essential Oil on Hepatic Tissues of Mice Model Exposed to Inorganic Lead Salt 薤白精油对暴露于无机铅盐的小鼠肝组织的预防性功效

Toxicology International Pub Date : 2023-11-03 DOI: 10.18311/ti/2023/v30i4/32343

Kusum Sharma, Veena Sharma

{"title":"Prophylactic Efficacy of Allium sativum Essential Oil on Hepatic Tissues of Mice Model Exposed to Inorganic Lead Salt","authors":"Kusum Sharma, Veena Sharma","doi":"10.18311/ti/2023/v30i4/32343","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/32343","url":null,"abstract":"Hypothesis: Lead is a pervasive environmental pollutant that is major threat for human health. Allium sativum essential oil could impart possible protection from Lead Nitrate (LN) as it contains organosulfur compounds which possess various pharmacological potential including antioxidant, anti-inflammatory, anticancer, anti-apoptotic and other activities as well. Parameters Studied: The ameliorative role of Allium sativum essential oil on hepatic tissue damage caused by Lead nitrate was evaluated through oxidative stress, biochemical parameters, oxidative Stress Index (OSI) and hepatic biomarkers. Methodology and Results: In this study, six groups of animals were taken. These groups were: control animals, toxicant treated animals (LN), LN + plant oil low and high dose treated animals, LN + silymarin treated animals and LN + vehicle oil control group. Lead nitrate exposure significantly decreased the antioxidant molecules mainly SOD, CAT, Gpx, GSH, GST, TPC and increased the lipid peroxidation content, Oxidative Stress Index (OSI), liver indices, Total Cholesterol Content (TCC) and biochemical parameters [ALT, AST, ALP] In addition to this, Lead nitrate increased the level of hepatic biomarkers such as cytp4502E1, 5’-nucleotidase, and γ-glutamyltranspeptidase and decreased the cytb5 content in hepatic tissues. Conclusion: Antioxidant activity of Allium sativum Essential Oil (ASEO) prevented oxidative stress and restored the level of liver indices, biochemical parameters, and hepatic biomarkers in Lead nitrate-intoxicated mice. Therefore, ASEO can be considered as a promising protective strategy against Lead nitrate-induced hepatotoxicity.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Acute Toxicity and the Effect of Sub-Lethal Concentration of Quinalphos on Protein and Lipid Peroxidation in Oreochromis niloticus 测定喹硫磷的急性毒性和亚致死浓度对黑线鲈蛋白质和脂质过氧化的影响

Toxicology International Pub Date : 2023-11-03 DOI: 10.18311/ti/2023/v30i4/33533

Chinnu Shaji, Suma M. Abraham, Joe Prasad Mathew

引用次数: 0

Chemical Induce Polycystic Ovarian Syndrome-Preclinical Animal Models 化学物质诱导多囊卵巢综合征--临床前动物模型

Toxicology International Pub Date : 2023-11-03 DOI: 10.18311/ti/2023/v30i4/34636

Aakansha Mishra, J. Kakadiya

{"title":"Chemical Induce Polycystic Ovarian Syndrome-Preclinical Animal Models","authors":"Aakansha Mishra, J. Kakadiya","doi":"10.18311/ti/2023/v30i4/34636","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/34636","url":null,"abstract":"Polycystic ovarian syndrome is a complex endocrine disturbance that leads to hyperandrogenism, disruption in the functioning of the Hypothalamic-Pituitary-Ovary (HPO) axis and multiple cysts in ovaries. To understand and study different treatment approaches of polycystic ovarian syndrome, there are several chemical-induced animal models available that mimic polycystic ovarian syndrome. These animal models are designed to closely resemble the characteristic symptoms. Polycystic Ovarian Syndrome’s key characteristics are changes in gonadotropin and sex steroid hormone, ovarian morphology, and metabolic characteristics. Direct hormone-regulated animal models are frequently utilized to study PCOS. Rodent animal model is often used which aims to replicate the key feature of human PCOS. Various endocrine-disrupting chemicals also makes a major role in the development of PCOS. In order to bridge the gap between basic research and clinical application in the field of PCOS, PCOS-induced models are essential tools for improving our understanding of the illness and evaluating innovative therapies. The review discusses various animal models used to induced PCOS by various inducers such as aromatase inhibitor inducer (letrozole), androgen excess inducer (dihydrotestosterone, dehydroepiandrosterone, testosterone), estrogen-induced (estradiol valerate), antiprogesterone (mifepristone), monosodium-L-glutamate, bisphenol-A and tributyltin chloride. This article contributed to underlying the current understanding and provides you a complete review that overall covers various aspects, including the impact of chemical-induced models, which also includes changes in the morphology of ovaries, gonadotropin as well as, and alterations in the level of various sex steroid hormone profile. Additionally it explores the metabolic abnormalities caused by various chemical-inducers used to induce PCOS in animal. The objective of this review is to provide a comprehensive review about various chemical inducers which are responsible for the development of PCOS.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"1155 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sub-Acute Toxicity Study of Hydro-Alcoholic Extracts of Sansevieria cylindrica and Plumeria obtusa in Wistar Albino Rats Wistar 白化大鼠对圆柱桑和钝顶梅水醇提取物的亚急性毒性研究

Toxicology International Pub Date : 2023-10-31 DOI: 10.18311/ti/2023/v30i4/31062

S. Shewale, V. Undale, V. Addepalli, Vrushali Bhalchim, S. Desai, Vikas Wawale, M. Shelar, Shubham Padole, Shital Satone, Shweta Lembhe, S. Parekh, P. Pujari

{"title":"Sub-Acute Toxicity Study of Hydro-Alcoholic Extracts of Sansevieria cylindrica and Plumeria obtusa in Wistar Albino Rats","authors":"S. Shewale, V. Undale, V. Addepalli, Vrushali Bhalchim, S. Desai, Vikas Wawale, M. Shelar, Shubham Padole, Shital Satone, Shweta Lembhe, S. Parekh, P. Pujari","doi":"10.18311/ti/2023/v30i4/31062","DOIUrl":"https://doi.org/10.18311/ti/2023/v30i4/31062","url":null,"abstract":"Sansevieria cylindrica Bojer ex. Hook (Asparagaceae) and Plumeria obtusa L. (Apocynaceae) are useful traditional medicines. The present study assessed the toxicity of a combination of hydro-alcoholic extract of aerial parts of both these plants through repeat administration in Wistar albino rats. This study is an extension of an acute toxicity study, wherein female rats were orally administered with a single dose of 2000mg/kg of both plant extracts. Sub-acute toxicity of both plants (200 and 400 mg/kg p.o.) was studied by daily dosing of Wistar rats of both sexes for 28 days. Toxicity, mortality, body weight changes, food and water intake, and neurological assessments were recorded. Hematological, biochemical, and urine analysis was done before and after dosing on day 29. Absolute and relative organ weight and histological evaluation were performed on day 29. The acute toxicity study revealed no lethal effects indicating that LD50 is greater than 2000mg/ kg. The sub-acute study observed a significant increase (p < 0.05) in body weight, feed, and water intake. The body weight gain in rats from the treatment group was non-significant (p > 0.05) compared to the control group. A significant (p < 0.05) increase in lymphocytes, granulocytes, RBC, platelet, creatinine, albumin, triglycerides, and alkaline phosphatase were also observed. The evaluation of different neurobehavior parameters showed a significant increase (p < 0.05). Histopathological analysis showed mild liver distortion in male rats in both treatment groups. Most of the significant observations were considered incidental findings indicative of low toxicity of plant extract during long-term use.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139306438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0