Thrita最新文献

{"title":"Effect of Metalloprotease Arazyme on the Expression of MMP2 and MMP9 Genes in Metastasis of Colon and Ovarian Cancer Cell Lines","authors":"G. Amjadi, K. Parivar, S. F. Mousavi, A. I. Fooladi","doi":"10.5812/thrita.100004","DOIUrl":"https://doi.org/10.5812/thrita.100004","url":null,"abstract":"Background: After cardiovascular diseases, cancer is the main cause of death in the United States, and its prevalence is continually increasing. Ovarian cancer is a fetal and common cancer among women and is the eighth common cancer in Iran. Colorectal cancer is known as the second and fourth common cancer in Iranian women and men, respectively. Arazyme is a metalloprotease with strong antitumor effects on tumor cells. Objectives: This study aimed at studying the effect of metalloprotease arazyme in vitro on the expression of MMP2 and MMP9 genes, causing metastasis in ovarian and colon cancer. Methods: Bacterial strains and cell lines, the construction of an expression vector, and preparation of recombinant protein were done. Then, they were evaluated by Western blot, cell culture, cell viability assay, and reverse transcriptase-polymerase chain reaction. Results: The effects of arazyme on ovarian and colon cell lines were assessed by the MTT assay showing that the viability of cancer cells treated with arazyme decreased significantly in comparison with control cells. Also, RT-PCR showed that the expression of MMP2 and MMP9 genes decreased after treatment with arazyme, which was significant when compared to the results of pre-treatment. Conclusions: In this study, the results showed that the use of arazyme protein as a bacterial anti-protease can play a significant role in reducing the expression of metastatic genes. According to numerous studies on the role of bacterial proteases in the process of metastasis in recent years, this method can be considered as a therapeutic approach in reducing the metastatic process.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90446209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Sexual Skills Training with an Eclectic Approach for Improving Sexual Function in Iranian Women","authors":"Mehrnoush Giahi Yazdi, N. Sedrpoushan, G. Dastjerdi, M. Sorbi, F. Sepehri","doi":"10.5812/thrita.55446","DOIUrl":"https://doi.org/10.5812/thrita.55446","url":null,"abstract":"Background: Having a proper sexual function is one of the most important factors in improving marital life quality and family relationships in married people. There are a few appropriate interventions for developing the quality of sexuality in the life of married women in Iran. Objectives: Thus, this study aimed to determine the effectiveness of sexual skills training with an eclectic approach in sexual function in Iranian women. Methods: This study was a randomized single-blinded clinical trial conducted from April to September 2015 on 30 women referring to counseling centers of Yazd selected with the purposeful sampling method. Participants were randomly assigned to experimental or control groups of 15. The experimental group received sexual skills training in nine sessions of 90 min (two sessions per week) but the control group remained on the waiting list. The data were collected before the intervention and five weeks afterward using a demographic form and Female Sexual Function index (FSFI-19). We used SPSS-21 to analyze data by Kolmogorov-Smirnov (K-S) test, chi-square test, t-test, and analysis of covariance (ANCOVA) at the P = 0.05 level. Results: The results showed that sexual skills training with an eclectic approach significantly increased sexual function and its subscales such as sexual desire and stimulation compared to the control group (P < 0.01). However, there was no significant difference between the two groups in lubrication and orgasm subscales. Conclusions: It can be deduced that sexual skills training has an effective role in promoting sexual function in married women. Thus, this cost-effective therapy can be used to increase the sexual quality of women.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74644892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem-cell Therapy in Human Osteoarthritis: A Debate","authors":"E. Z. Ziabari, A. Ebrahimi, S. Ashkani-Esfahani, M. Razi","doi":"10.5812/thrita.101358","DOIUrl":"https://doi.org/10.5812/thrita.101358","url":null,"abstract":"Stem-cell Therapy in Human Osteoarthritis: A Debate Elaheh Ziaei Ziabari 1, , Alireza Ebrahimi 3, , Soheil Ashkani-Esfahani 4 and Mohammad Razi 5 Rothman Institute, Thomas Jefferson University, Philadelphia, USA School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA Department of Orthopaedic Surgery, Iran University of Medical Sciences (IUMS), Tehran, Iran Corresponding author: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. Email: alireza.ibrahim92@gmail.com","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75109850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and Behavioral Alterations Following hADSCs Intravenous Administration in Alzheimer’s Rat Model","authors":"Milad Kazemiha, A. Sarveazad, F. Moradi, Farnaz Ramezanpour, M. Vosoogh, Maryam Doshmanziari, Marjan Shariatpanahi, M. Eftekharzadeh","doi":"10.5812/thrita.99975","DOIUrl":"https://doi.org/10.5812/thrita.99975","url":null,"abstract":"Background: Alzheimer’s disease (AD) is a progressive cognitive disorder that is generally age-related. Although there has been great research focusing on this disease, there is still a lack of reliable therapeutic methods. Amyloid- β (A β ) peptide has a critical function in neuropathology of AD. Stem cell therapy provides treatment by improving the neuronal system in neurodegenerative disorders. Human adipose-derived stem cells (hADSCs) are the most appropriate sources of stem cells due to their safety, high pro-liferative potential, and easy isolation. Objectives: Thepresentstudywasdesignedtoevaluatethehistologicalandbehavioralalterationsafterintravenousadministration of hADSCs in the AD rat model. Methods: In this study, 32 male rats were used in four groups, as follows: control, sham, AD rat model, and hADSCs-treated group. We used Morris Water Maze (MWM) for evaluating behavioral changes and Nissl staining for determining the histological studies. Results: In this study, the AD model was confirmed by behavioral and histological analysis. Behavioral results showed that the spatial memory improved after hADSCs injection in the AD rat model while the time spent in the target quadrant was significantly higher in the hADSCs-treated group than in the AD rat model group. On the other hand, the number of dead cells significantly decreased in the hADSCs-treated group as analyzed by Nissl staining. Conclusions: Our findings revealed that hADSCs could transfer into the brain and improve memory and neuronal damage in the AD rat model.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89285532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Positive Psychotherapy on the Well-Being and Glycemic Control of Patients with Type 2 Diabetes: A Randomized Controlled Trial","authors":"M. Sorbi, M. Rahmanian, R. Azizi","doi":"10.5812/THRITA.63926","DOIUrl":"https://doi.org/10.5812/THRITA.63926","url":null,"abstract":"BackgroundDiabetes is one of most widespread diseases that disturb the mental and physical health of patients.ObjectivesThe aim of study was searching a therapeutic method to enhance the diabetic’s health by supposing the effectiveness of positive psychotherapy on the well-being and glycemic of patients with type 2 diabetes in Iran.MethodsIn a randomized, single-blinded clinical-trial study, from January 2016 to February 2017, a total of 30 type 2 diabetic patients of Yazd Diabetes Research Center, with purposeful sampling method, were selected randomly and put in two groups (Experimental and Control groups). While participants of both groups continued their drug therapy, the experimental group received 10 sessions of 90 minutes (a session per week) group positive psychotherapy while the control group remained on the waiting list. Data were gathered before the intervention and two weeks after intervention. At the end of the treatment sessions three of the experimental group (n = 12) and two of the control group (n = 13) were excluded from the study for their absence in post-test. Data were gathered by demographic form, general well-being questionnaire (GWB), and Glycemic test (HbA1c). SPSS software, version 16 with P = 0.05 level, Kolmogorov-Smirnov (K-S), chi-square, and analysis of covariance (ANCOVA) were used for analyzing data.ResultsThe results showed that positive psychotherapy significantly increases mental well-being, and its subscales in comparison with the control group (P < 0.01). Also, other results showed that glycemic in the experimental group in comparison with the control group significantly decreased (P < 0.05).ConclusionsIt can be deduced that positive therapy intervention can be effective in mental well-being and to some extent, blood glucose of diabetics. So, this therapy can be used when diabetic patient’s psychological factors should be carefully considered.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84877269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Simultaneous Eight-Week Exercise and Crocin Usage on the Oxidation and Anti-oxidation Indices of Male Rats’ Testicles Subjected to Apoptosis","authors":"Kiyanoosh Darash, M. Ghanbarzadeh, M. Nikbakht","doi":"10.5812/THRITA.90438","DOIUrl":"https://doi.org/10.5812/THRITA.90438","url":null,"abstract":"BackgroundStudies have shown that physical activity and the consumption of the medicinal herb saffron affect patients who have testicular problems and those who are prone to apoptosis. In this study, the effects of simultaneous eight-week exercise and crocin usage on the oxidation and anti-oxidation indices of male rats’ testicles subjected to apoptosis were investigated.MethodsIn this experimental study, 56 Wistar rats were randomly divided into 7 groups. The groups receiving supplement, exercise, and supplement received oral doses of 10 and 50 mg per kg of body weight for 8 weeks. The control group received only normal saline and the same amount of supplement as gavage. Fourty-eight hours after the last exercise session and after 10-12 hours of fasting overnight, blood samples were collected from animals after anesthesia and measured by dye-matase and catalase activity and the concentration of malondialdehyde in testicular tissue was measured. Statistical analysis was performed using SPSS software version 20 (P < 0.05).ResultsThe results showed that in all groups except the sick group through exercise and receiving a dose of 10 and 50 mg per kg crocin, dismutase and catalase indices increase, and the malondialdehyde index decreased and the T4 (doxorubicine + 10 mg crocin) group significantly increased in the antioxidant index and decreased in oxidative index (P < 0.05).ConclusionsThe findings of this study showed that endurance training and crocin dose-dependent injections by decreasing the toxicity of doxorubicin reduced the oxidative stress and free radicals and made good compatibility in the antioxidant system of testicular tissue in male rats.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76939041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variance of Maternal-Fetal Attachment in Pregnant Women Based on Personality Traits","authors":"M. Zolfaghari, A. Naseri, Sadra Khosravi, M. Fatemi","doi":"10.5812/THRITA.12657","DOIUrl":"https://doi.org/10.5812/THRITA.12657","url":null,"abstract":"BackgroundMaternal-fetal attachment (MFA) is the first relationship between a mother and her child, which generally begins during pregnancy. The maternal psychological state affects fetal growth and health. MFA is associated with the mental and psychological condition in pregnant women.ObjectivesThe aim of this study was the estimation of the variance of MFA, according to the personality traits in pregnancy.MethodsUsing a descriptive correlational study design, we studied pregnant women who were more than 24 weeks pregnant and were referring to the obstetric ward of the Kowsar Hospital, Shiraz, Iran, during May to June 2015. The sample size was estimated to be 200 women by Morgan’s table. A total of 29 participants were removed due to incomplete questionnaires, and 171 participants were selected. Data collection tools included demographic obstetric questionnaires, including obstetric information and demographic data, Cranly’s MFA scale, and NEO personality scale. Data were analyzed by the Pearson correlation coefficient and multiple regression, by using SPSS version 16.ResultsFindings from data analysis demonstrated a significant correlation between components of NEO personality with maternal-fetal attachment. Extroversion index (P < 0.001, R = -0.51) had the most significant relationship with MFA. Almost 33% of the variance in maternal-fetal attachment was predicted by personality trait. The extraversion (β = 0.48, P < 0.001) is the highest level of prediction. Neuroticism (β = 0.175), openness to experience (β = 0.131) and conscientiousness (β = 0.237), predicted the maternal-fetal attachment positively. Agreeableness (β = -0.026) was not predicted in the maternal-fetal attachment. In demographic variables, they were not predicted of MFA. There was a significant correlation between parity and maternal-fetal attachment (R = 0.190, P = 0.013).ConclusionsPersonality traits (except agreeableness) predict MFA for pregnant women. In addition, the psychological health of the mother and baby can be improved by identifying mothers based on personality traits, and therefore, by promoting behavioral attachment, health care providers should identify mothers who need additional psychological support during their pregnancy.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76841851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of Curcumin with One Subunit of Monoamine Oxidase-B in Comparison with Safinamide: An In Silico Study","authors":"S. Nadjafi, Nasrin Hosseini, M. Farhadi, F. Khojasteh","doi":"10.5812/THRITA.90380","DOIUrl":"https://doi.org/10.5812/THRITA.90380","url":null,"abstract":"Curcumin is a plant derivative with biological effects, including potential for the treatment of Parkinson’s disease (PD). It is known that monoamine oxidase B (MAO-B) is involved in PD due to its role in the degradation of various neurotransmitters like dopamine, the main declined factor in PD. Since MAO-B inhibitors (e.g. safinamide) are used as the support for the treatment of PD, we planned to evaluate the in silico interaction of curcumin with one subunit of the MAO-B enzyme in comparison with safinamide. The crystal structure of human MAO-B (PDB entry code 3PO7) was selected from the Protein Data Bank (https://www.rcsb.org). The molecular structures of curcumin (CID: 969516) and safinamide (CID: 131682) were obtained from PubChem (https://pubchem.ncbi.nlm.nih.gov). Chimera 1.8, AutoDock Tools-1.5.6 software and AutoDock4 software were used for this in silico study. The results revealed that the binding energies (ΔG)s of the conformations of curcumin and safinamide, showing the best down ΔG, were -11.15 kcal/mol and -11.09 kcal/mol, respectively. Moreover, the inhibition constants (Ki)s of both ligands were near quantities. Hence, it may suggest that curcumin and safinamide form nearly similar stability with the subunit of the MAO-B enzyme. More experimental studies may reveal the similarity of curcumin with safinamide about the inhibitory effect on MAO-B.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84375297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is Left Unsaid About Semantic, Episodic and Working Memory: L2 Cognitive Control Accounts for Individual Differences and Gender Differences","authors":"Behzad Nasirpour, M. Bagheri, B. Jameie","doi":"10.5812/THRITA.85857","DOIUrl":"https://doi.org/10.5812/THRITA.85857","url":null,"abstract":"Background: Prefrontal cortex (PFC) is believed to manipulate cognitive control and mnemonic process to overcome interference. The interaction among memory, cognitive control, and language learning is still under debate. Objectives: This study investigated how episodic, semantic, and working memory (WM) as linguistically dependent retrieval cues can entail the interference process among EFL (English as a foreign language ) individuals. Methods: Combining the samples from five testing sectors in Shiraz, Iran, yielded data from 78 adult participants (33 males; 52 EFL independent users; 26 basic users; mean age 34.63). The participants took the test package of selection mechanism measures in resolving interference (SMMRI), including selection in semantic/episodic memory and WM/priming (backward printed reading comprehension). Both descriptive statistics (mean scores, standard deviations) and inferential statistics (Correlations, t-tests, interface resolution (IR)) were performed using SPSS software version 25.0. Results: There was a downward trend in the order of magnitude from episodic (OLD-NEW) toward semantic (high-selection) toward WM/priming. Also, there was a moderate/high correlation among the aforementioned memories. There was a significant difference in IR-scores between basic and independent users. Conclusions: Hierarchical process of compromises between L2 (Second language) memories indicated that the interactions of different cognitive sub-components played a central executive role. The difference between IR-scores revealed that training on WM tasks, which demanded higher activations in the PFC, could enhance the ability in resolving interference. Retrieval cues have limited concomitant memory capacity that integrate morphological, semantic, and syntactic features. However, men outperformed females in semantic selection, no significant gender difference was seen in episodic selection.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76322591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Carvacrol on the Expression of P53 Protein in Testicular Tissue and Spermatogenesis of Rats Treated with Methamphetamine","authors":"M. Farhadi, B. Jameie, Parisa Honarkaran, Raheleh Mollajani, M. Jameie, Melikasadt Jameie","doi":"10.5812/THRITA.88303","DOIUrl":"https://doi.org/10.5812/THRITA.88303","url":null,"abstract":"The application of traditional and industrial addictive drugs is expanding in the developed societies. Carvacrol is widely used in traditional medicine, and methamphetamine, directly and indirectly, affects all organs. According to the mechanism of methamphetamine, the therapeutic effects of antioxidants were recently considered in order to reduce methamphetamine abuse outcomes. The current study aimed at evaluating the anti-apoptotic effect of carvacrol on spermatogenesis of rats treated with methamphetamine. Thus, 32 adult male rats were randomly divided into four groups: positive control, negative control, sham, and experimental. Spermatogenic cells and testis tissue were evaluated using hematoxylin and eosin staining technique and the expression of P53 was assessed by Western blotting as a factor to induced apoptosis. The current study data showed that methamphetamine significantly reduced spermatogenic cells in the positive control group, while carvacrol increased these cells in the experimental group. Also, carvacrol decreased P53 expression in the experimental group compared with the positive control group (P < 0.05). Therefore, carvacrol had an anti-apoptotic effect by suppressing P53 protein expression. Altogether, carvacrol can reduce some of the common symptoms related to methamphetamine abuse including infertility and induction of apoptosis.","PeriodicalId":23167,"journal":{"name":"Thrita","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75899403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}