Topics in companion animal medicine最新文献

{"title":"Evaluation of canine and feline tumors in Morocco: Results of a prospective observational study of 250 cases (2020-2023).","authors":"Nadia Laissaoui ,&nbsp;Yolanda Millán ,&nbsp;Daniela Simon Betz ,&nbsp;Meryem El Mrini ,&nbsp;Najat Lamalmi ,&nbsp;Rahma Azrib ,&nbsp;Noursaid Tligui","doi":"10.1016/j.tcam.2024.100929","DOIUrl":"10.1016/j.tcam.2024.100929","url":null,"abstract":"<div><div>This work aimed to evaluate the histologic tumor types and patient characteristics of canine and feline neoplasias in Morocco. In a prospective, observational study, 250 tumor samples were collected from veterinary clinics in Morocco. For each case, breed, age, sex, neuter status, and tumor site and histotype were analyzed. Among the 250 tumors, 201 (80.4%) were from dogs and 49 (19.6%) from cats. Most common tumor location in dogs was the reproductive tract (36.8%), followed by skin and soft tissues (27.4%) and mammary gland (14.9%). In cats, most tumors originated from the skin and soft tissues (55.1%), followed by mammary gland (20.4%) and oral cavity (14.3%). Commonest canine histologic tumor types were transmissible venereal tumor (CTVT<span><span>¹</span></span>) (31.8%) and mammary carcinoma (14.4%). Squamous cell carcinoma (34.7%) and mammary carcinoma (20.4%) represented the most frequent feline neoplasms. Median age of dogs and cats at diagnosis was 7 and 10 years, respectively. Gender distribution was even in dogs; in cats, females were overrepresented. Most cats and about half of dogs were mixed-breed. The evaluated canine and feline neoplasias show differences in their tumor types and patient characteristics when compared to populations from other countries, in part based on the high number of CTVT and - possibly solar-induced –feline skin tumors.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100929"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological biomarker patterns, venom detection and venom proteomics in canine Vipera berus envenomation","authors":"Tove V. Nicolaysen ,&nbsp;Hannah J. Harjen ,&nbsp;Heidi S. Lund ,&nbsp;Bente K. Sævik ,&nbsp;Runa Rørtveit ,&nbsp;Karin E. Zimmer","doi":"10.1016/j.tcam.2024.100930","DOIUrl":"10.1016/j.tcam.2024.100930","url":null,"abstract":"<div><div><em>Vipera berus</em> (<em>V. berus</em>) bites are associated with high morbidity, including kidney injury, in dogs. Although antivenom is often used and perceived effective to treat this type of snakebite, it is costly and associated with adverse events and specific diagnostics for this type of snakebite are lacking. We sought to improve diagnostics in <em>V. berus</em> envenomation by using currently available tools, including evaluating urinary albumin as a biomarker for snakebite-associated kidney injury. Additionally, we planned to adapt a method from human medicine for venom detection in clinical samples from bitten dogs and describe the composition of Norwegian <em>V. berus</em> venom. Serum biochemical analytes and urine albumin (ELISA) were measured in samples collected at 24 hours and two weeks after bite in 29 envenomated dogs. An adapted ELISA was applied to detect venom in urine and plasma collected from 25 cases between presentation and 24 hours after bite, using a commercial antivenom as the capture and detection antibody. Proteomic analysis of venom collected from 11 <em>V. berus</em> was performed using liquid chromatography-tandem mass spectrometry. Elevated serum C-reactive protein, creatine kinase, and aspartate aminotransferase were common for the case group. Although no case dogs showed acute kidney injury with azotemia and/or reduced urine output, elevated urinary albumin concentrations may indicate early or mild kidney injury in some case dogs. The venom ELISA detected positive signals in both plasma and urine for up to 24 hours after bite. However, with false positives detected in plasma, urine seemed to be the most appropriate body fluid for this assay. The venom proteome identified L-amino acid oxidases as the dominant component. In conclusion, serum biochemical and urinary albumin analyses are useful tools for evaluating canine <em>V. berus</em> envenomation. The venom ELISA is proposed as a promising tool for studies of <em>V. berus</em> envenomation and future diagnostic test development. Venom from the studied Norwegian <em>V. berus</em> was shown to differ considerably from previous reports from other countries, implying geographical variation in composition.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100930"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report on the presence of a common pathogenic variant in the PKD1 gene, causing polycystic kidney disease, in a series of related Sphynx cats","authors":"Joanna Nowacka-Woszuk ,&nbsp;Marta Sobczak ,&nbsp;Zuzanna Sawicz ,&nbsp;Marlena Wiench ,&nbsp;Hanna Nowaczyk-Rzeszotalska ,&nbsp;Malwina Kolodziej ,&nbsp;Marek Switonski","doi":"10.1016/j.tcam.2024.100928","DOIUrl":"10.1016/j.tcam.2024.100928","url":null,"abstract":"<div><div>Polycystic kidney disease (PKD) is the most common monogenic disease in cats, especially in Persian and related breeds. In this report we describe for the first time PKD in two related families (full sibling tomcats) of Canadian Sphynx cats. Altogether fourteen cats, including five kittens in each family, were investigated using ultrasonography. Cysts were observed in both tomcats and in five kittens (two and three in the litters). The number of cysts in a single kidney varied from one to six. A molecular search for the causative dominant pathogenic variant (Felis_catus_9.0, NC_018738.3: chr E3:g.42858112C&gt;A (c.9882C&gt;A)) in the <em>PKD1</em> gene showed full concordance with clinical investigations: all cats with cysts in their kidneys were heterozygous (C/A) for this variant.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100928"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traumatic brain injury in companion animals: Pathophysiology and treatment","authors":"Molly Wart ,&nbsp;Thomas H. Edwards ,&nbsp;Julie A. Rizzo ,&nbsp;Geoffrey W. Peitz ,&nbsp;Armi Pigott ,&nbsp;Jonathan M. Levine ,&nbsp;Nicholas D. Jeffery","doi":"10.1016/j.tcam.2024.100927","DOIUrl":"10.1016/j.tcam.2024.100927","url":null,"abstract":"<div><div>Traumatic brain injuries (TBI) are common in dogs and cats that have sustained head trauma from a variety of causes. In moderate to severe TBI, damage from both the primary and secondary injuries can be life-threatening. TBI management may be further complicated by concurrent injuries in polytrauma patients. Thorough initial and serial examinations are key in detecting neurologic changes quickly and guiding treatment. Intensive treatments such as nursing care, fluid therapy, hyperosmolar agents, analgesia, sedation, anticonvulsants, oxygen supplementation, surgery, and rehabilitation may be employed in TBI management. Prognostication resources for an individual patient are limited and a perceived poor prognosis may worsen clinical outcomes. In this paper, we review the pathophysiology of TBI, identification, injury stratification and prognosis of patients with TBI as well as propose treatment and monitoring recommendations for companion animals based on severity of TBI.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100927"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mandibular salivary gland plasma cell tumor in a dog","authors":"Erasmia Smyroglou ,&nbsp;Taiana Costa ,&nbsp;Simela-Maria Tachtsoglou ,&nbsp;Athanasios Grammenopoulos ,&nbsp;Kyriaki Makri ,&nbsp;Theodoros Kalogirou ,&nbsp;Lysimachos G. Papazoglou ,&nbsp;Mathios E. Mylonakis","doi":"10.1016/j.tcam.2024.100926","DOIUrl":"10.1016/j.tcam.2024.100926","url":null,"abstract":"<div><div>An 8-year-old, intact male, Jack Russell Terrier dog was admitted with a 10-day history of an enlargement in the right submandibular area. Clinical examination revealed a subcutaneous, solid, firmly attached and painless mass (3 × 2 cm) located in the area of the right mandibular salivary gland, in an otherwise seemingly healthy dog. Fine-needle-aspiration cytology suggested a round cell neoplasia, most consistent with a plasma cell tumor. Histopathologic and immunohistochemical (MUM1) examination, following surgical excision of the mass, confirmed the diagnosis of a plasma cell tumor embedded in the right mandibular salivary gland. Clinical staging reasonably excluded a disseminated plasma cell tumor. The dog remains healthy and in complete remission 23 months following surgical excision of the mass. Albeit rare, extramedullary plasma cell tumors can affect the salivary glands of dogs.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100926"},"PeriodicalIF":1.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac embryonal rhabdomyosarcoma in a domestic shorthair cat","authors":"Anahi Souza Silva ,&nbsp;Isabela Leite Doretto ,&nbsp;Isabel Luana de Macêdo ,&nbsp;Liz de Albuquerque Cerqueira ,&nbsp;Christine Souza Martins ,&nbsp;Cristiano Barros de Melo ,&nbsp;Márcio Botelho de Castro","doi":"10.1016/j.tcam.2024.100925","DOIUrl":"10.1016/j.tcam.2024.100925","url":null,"abstract":"<div><div>Rhabdomyosarcomas (RBMs) are rare neoplasms arising from striated muscle tissues in domestic animals and are exceptionally uncommon, affecting the heart of domestic cats. A case of cardiac embryonal RBM was detected in a two-year-old female domestic shorthair cat. The cat exhibited dyspnea, anorexia, pericardial and pleural effusions, ultimately succumbing to severe respiratory arrest. Gross post-mortem examination revealed a firm yellow-tan mass measuring 2.5 × 1.5 cm protruding from the right atrium, along with metastatic nodules of various sizes dispersed throughout the liver, spleen, and lungs. On histology, the right atrium exhibited a highly cellular infiltrative and vascularized mass predominantly composed of spindle and small round cells arranged in dense cellular bundles supported by a scant fibrous stroma interspersed with scarce regions of loosely myxoid stroma. Comparable histological features were noted in the metastatic lesions. Immunohistochemical analysis revealed positive immunolabeling for vimentin, desmin, MyoD1, and fast myosin, while no immunostaining was observed for smooth muscle actin, chromogranin, and S100. Histological and immunohistochemical features supported the diagnosis of metastatic cardiac embryonal RBM spindle cell variant in the cat. Cardiac embryonal RBMs should be included in the differential diagnosis of cardiac and thoracic neoplasms in domestic cats presenting with clinical signs indicative of cardiac arrest or sudden death.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100925"},"PeriodicalIF":1.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haematogenous polyostotic osteomyelitis caused by Serratia marcescens in a cat","authors":"Luca Ciammaichella ,&nbsp;Veronica Cola ,&nbsp;Armando Foglia ,&nbsp;Stefano Zanardi ,&nbsp;Carmit Chalfon ,&nbsp;Chiara Tassani ,&nbsp;Giancarlo Avallone ,&nbsp;Erika Monari ,&nbsp;Luciano Pisoni ,&nbsp;Sara Del Magno ,&nbsp;Francesco Dondi","doi":"10.1016/j.tcam.2024.100924","DOIUrl":"10.1016/j.tcam.2024.100924","url":null,"abstract":"<div><div>A 2-year-old female Bengal cat was referred for acute right forelimb lameness one month after ovariectomy. Physical examination revealed multifocal pain on bone palpation and fever. Radiographs showed mixed lytic-proliferative polyostotic diaphyseal osteopathy of multiple bone segments. Histopathologic evaluation of bone biopsies showed severe chronic pyogranulomatous osteomyelitis and multidrug-resistant <em>Serratia marcescens</em> was cultured. Antibiotic therapy with piperacillin-tazobactam was administered for 60 days, based on susceptibility testing. Lameness and bone pain resolved within 15 days, and radiographs after 30 days showed decreased bone lysis. At a 6-month recheck, the cat recovered completely, and only bone remodelling was evident on radiographs.</div><div>Multifocal bacterial haematogenous osteomyelitis (HO) caused by <em>Serratia marcescens</em> was diagnosed in an adult immunocompetent cat. HO is infrequently reported in dogs and cats with young and immunocompromised patients being most at risk. Prior ovariectomy and anaesthesia may have predisposed the cat to the development of a hospital-associated infection (HAI), as other aetiologies or predisposing causes for osteomyelitis were reasonably excluded. <em>Serratia marcescens</em> is a gram-negative bacterium recently reported as responsible for human and veterinary HAIs, although it has never been stated for HO in small animals. Early recognition and antibiotic therapy led to good outcome in this case.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100924"},"PeriodicalIF":1.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The peculiar clinical presentation and diagnostic results of a case of widely metastatic cholangiocellular carcinoma in a Siberian Husky dog","authors":"Hannah Reichert ,&nbsp;Alexandra Macleod ,&nbsp;Leslie Sharkey ,&nbsp;Cornelia Peterson","doi":"10.1016/j.tcam.2024.100923","DOIUrl":"10.1016/j.tcam.2024.100923","url":null,"abstract":"<div><div>A six-year-old spayed female Siberian Husky dog was evaluated for acute cervical pain and ataxia, respiratory changes, and a two-month history of weight loss. Antemortem diagnostics included complete physical and neurologic examination, complete blood count, serum biochemistry, cervical and thoracic radiographs, abdominal ultrasound (AUS), and splenic and lymph node cytology. Abnormalities included C6-T2 myelopathy, mildly elevated hepatocellular enzymes, sternal lymphadenopathy, ill-defined hepatosplenic nodules, and multifocal lymphadenopathy, with concern for metastatic carcinoma cytologically. Humane euthanasia was elected, and a diagnostic necropsy was performed. Tissues were formalin-fixed and routinely processed before hematoxylin and eosin staining. Affected hepatic tissues were further evaluated by trichrome staining and immunolabeling for cytokeratins 7 and 19 (CK7 and CK19). Expanding and compressing the left lateral liver lobe was a large, infiltrative, tan-yellow, umbilicated mass. The remainder of the hepatic parenchyma contained up to 100 additional similarly appearing intrahepatic masses, and additional masses were observed grossly within the gallbladder, spleen, mesentery, urinary bladder, diaphragm, lungs, pleura, and sternal lymph nodes. Histology of the primary and metastatic lesions was consistent with cholangiocellular carcinoma (CC) with varying degrees of anaplasia, and a marked scirrhous response was confirmed with trichrome staining. The primary hepatic mass was CK19+/CK7- with immunolabeling. Cervical pain represents an atypical presentation for widely metastatic CC and is a suspected sequel of vertebral metastasis or embolic spinal disease. The primary hepatic mass was not captured by routine AUS, and immunolabeling of the primary lesion was negative for CK7 expression, highlighting challenges to antemortem diagnostics and poor cellular differentiation, respectively.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100923"},"PeriodicalIF":1.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilateral testicular seminoma in a captive Siberian chipmunk (Tamias sibiricus)","authors":"Morena Bernadette Wernick ,&nbsp;Caroline Voutaz ,&nbsp;Nicolas Kühn ,&nbsp;Judith Howard","doi":"10.1016/j.tcam.2024.100922","DOIUrl":"10.1016/j.tcam.2024.100922","url":null,"abstract":"<div><div>A 5-year-old male Siberian chipmunk (<em>Tamias sibiricus</em>) was presented with marked enlargement of the left testicle. Differential diagnoses included testicular neoplasia such as seminoma, interstitial cell neoplasia, Sertolli cell neoplasia and teratoma or orchitis. Fine needle aspiration of the left testicle was performed under general anesthesia and cytologic findings were consistent with seminoma. Following bilateral orchiectomy, both testicles were examined histologically, revealing bilateral diffuse seminoma. The chipmunk made an uneventful recovery from anesthesia and was treated with analgesics. Follow up two years after surgery, no clinical signs were observed. Seminomas are rare in chipmunks, but orchiectomy may result in long-term disease-free survival as described in other myomorph rodents.</div></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100922"},"PeriodicalIF":1.3,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of metastatic apocrine sweat gland adenocarcinoma in a cat with association of surgical resection and angular oris reconstruction, extensive lymphadenectomy, electrochemotherapy and systemic chemotherapy","authors":"Pedro Antônio Bronhara Pimentel ,&nbsp;Isabella Oliveira Almeida ,&nbsp;Bruna Voltolin de Sena ,&nbsp;Sarah Kowalczyk ,&nbsp;Geovanni Dantas Cassali ,&nbsp;Rodrigo dos Santos Horta","doi":"10.1016/j.tcam.2024.100921","DOIUrl":"10.1016/j.tcam.2024.100921","url":null,"abstract":"<div><p>Apocrine sweat gland adenocarcinoma (ASGAC) is a rare skin carcinoma in cats. In some cases, this tumor occurs in anatomical sites of challenging wide surgical resection, which increases the need for adjuvant therapies for residual disease. These include radiotherapy or electrochemotherapy (ECT), local treatments for invasive tumors in companion animals often associated with surgery. However, the current literature for ASGAC treatment is limited and there are no reports of ECT as an adjuvant therapy. In this case report, we account for the case of an 11-year-old cat with a history of a non-ulcerated recurrent ASGAC, measuring 1.3 × 1.0 cm, located by the rostral mandibular region. Surgical resection included the angularis oris axial flap for facial reconstruction associated with electroporation of the surgical bed and, post-operatively, in the surgical scar. Histopathological results confirmed the presence of a recurrent ASGAC. Immunostaining revealed cyclooxygenase 2 (COX-2) expression with a score of 6, 50 % positivity in Ki-67, and positive for pan-cytokeratin (PCK AE-1/ AE-3). A selective COX-2 inhibitor was initiated along with systemic chemotherapy with chlorambucil. The local approach including surgery and ECT was chosen due to the unfavorable anatomical site for extensive resection and the unavailability of radiotherapy. Subsequently, carboplatin chemotherapy was required due to metastasis in the mandibular lymph node. This case report supports the effectiveness of a multimodal treatment including surgery, ECT, and chemotherapy in a cat with recurrent ASGAC.</p></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":"63 ","pages":"Article 100921"},"PeriodicalIF":1.3,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}