Toxicologic Pathology最新文献

Microscopic Findings in Seminal Vesicles of Control Rats Mated to Females From Fertility and Early Embryonic Development Studies. 从生育和早期胚胎发育研究中与雌性交配的对照大鼠精囊的显微发现。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-10-06 DOI: 10.1177/01926233251375447

Emmanuelle Balme, Bernd Baier, Katja Hempel, Susanne Mueller, Sydney Mukaratirwa, Thomas Nolte

引用次数: 0

Toxicologic Pathology Forum*: Opinion on the Future of Histopathology Using Whole Slide Images in Toxicologic Pathology of Preclinical Studies and Its Successful Implementation in Compliance With Good Laboratory Practice-Yes, We Are There! 毒理学病理论坛*：关于在临床前毒理学病理研究中使用全幻灯片图像的组织病理学的未来及其在符合良好实验室规范的情况下的成功实施的意见-是的，我们在那里！

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-09-28 DOI: 10.1177/01926233251366270

Bob Thoolen, Alys Bradley, Nikolas Stathonikos, Paul J van Diest

引用次数: 0

Reliable Identification of Alpha2u-Globulin and Lysozyme Accumulation in Rat Kidney by Label-Free MALDI Mass Spectrometry Imaging. 无标记MALDI质谱成像可靠地鉴定大鼠肾脏中α - u-球蛋白和溶菌酶积累。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-09-10 DOI: 10.1177/01926233251368855

Stefania-Alexandra Iakab, Heike Antje Marxfeld, Florian M Richter, Alexander Geisel, Yasemin Ucal, Tobias Bausbacher, Christina Henser, Sibylle Gröters, Carsten Hopf

引用次数: 0

Calculating Veterinary Clinical Pathology Reference Intervals for Research at the Integrated Research Facility, NIAID: A Practical Application of Data Partitioning and Statistical Methodology. 计算兽医临床病理参考区间的综合研究设施，NIAID：数据划分和统计方法的实际应用。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-08-23 DOI: 10.1177/01926233251352495

Daniel B Woodburn, Jeremy J Bearss, Camille M Lake, Natalie L Twilley, Whitney L Do, Joshua R Porter, Jing Qin, Kevin Footer, Sean Bartlinski, Joe Croghan

{"title":"Calculating Veterinary Clinical Pathology Reference Intervals for Research at the Integrated Research Facility, NIAID: A Practical Application of Data Partitioning and Statistical Methodology.","authors":"Daniel B Woodburn, Jeremy J Bearss, Camille M Lake, Natalie L Twilley, Whitney L Do, Joshua R Porter, Jing Qin, Kevin Footer, Sean Bartlinski, Joe Croghan","doi":"10.1177/01926233251352495","DOIUrl":"https://doi.org/10.1177/01926233251352495","url":null,"abstract":"The veterinary research community requires statistically robust reference intervals in which to contextualize the results of laboratory tests obtained in research studies. While published reference intervals are available for veterinary clinical practice, they typically do not account for differences in animal husbandry, variations in analytical equipment, and the diverse range of species encountered in a research setting. In addition, existing guidelines for statistical calculation of reference intervals do not address commonly encountered issues with data quality, sample size, research-induced population biases, and other impediments. In this manuscript, we document our pipeline to extract, partition, analyze, and statistically summarize in-house clinical pathology data for developing useful reference intervals to support research at the Integrated Research Facility at Fort Detrick (National Institute of Allergy and Infectious Diseases) and showcase a practical application of statistical methodology that can guide other facilities in their own determination of clinical pathology reference intervals.","PeriodicalId":23113,"journal":{"name":"Toxicologic Pathology","volume":" ","pages":"1926233251352495"},"PeriodicalIF":1.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathologic Findings in the Heart Associated With Telemetry Instrumentation in Beagle Dogs. 比格犬心脏与遥测仪器相关的组织病理学结果。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-08-19 DOI: 10.1177/01926233251364231

Rebecca Kohnken, Amanda Wilsey, Brandan Bird, Olivera Antic, Geena Jasiek, C Michael Foley

{"title":"Histopathologic Findings in the Heart Associated With Telemetry Instrumentation in Beagle Dogs.","authors":"Rebecca Kohnken, Amanda Wilsey, Brandan Bird, Olivera Antic, Geena Jasiek, C Michael Foley","doi":"10.1177/01926233251364231","DOIUrl":"10.1177/01926233251364231","url":null,"abstract":"Conscious telemetry-instrumented dogs are used during cardiovascular safety assessment in early drug development for the collection of cardiovascular function parameters such as electrocardiography (ECG), heart rate, mean arterial pressure, and cardiac contractility. To refine the use of animals for exploratory toxicology studies, these dogs may be repurposed into these studies following failure of their implanted device. Telemetry instrumentation involves surgical placement of a pressure catheter through the left ventricular apex of the heart. Slight variations in placement and extension of the catheter tip into the ventricular chamber lead to variability in cardiac pathology associated with the instrumentation. Reuse of these animals in toxicologic assessments therefore requires the pathologist to be aware of the spectrum of histologic changes that may occur because of telemetry instrumentation. This report describes cardiac findings in 12 telemetry-instrumented male dogs with implant duration ranging from 7 to 37 months and discusses considerations and recommendations for use of these animals.","PeriodicalId":23113,"journal":{"name":"Toxicologic Pathology","volume":" ","pages":"1926233251364231"},"PeriodicalIF":1.8,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicologic Pathology Forum*: Opinion on Qualitative Severity Descriptors to Express Magnitude of Changes in Clinical Pathology Endpoints in Nonclinical Toxicity Studies. 毒理学病理论坛*：关于表达非临床毒性研究中临床病理终点变化幅度的定性严重性描述符的意见。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-08-01 Epub Date: 2025-05-31 DOI: 10.1177/01926233251341271

N K Tripathi, L Ramaiah, T Arndt, L Cregar, A O Adedeji, D Meyer, J Whalan, A E Schultze

{"title":"Toxicologic Pathology Forum*: Opinion on Qualitative Severity Descriptors to Express Magnitude of Changes in Clinical Pathology Endpoints in Nonclinical Toxicity Studies.","authors":"N K Tripathi, L Ramaiah, T Arndt, L Cregar, A O Adedeji, D Meyer, J Whalan, A E Schultze","doi":"10.1177/01926233251341271","DOIUrl":"10.1177/01926233251341271","url":null,"abstract":"Clinical pathology endpoints are routinely assessed in nonclinical toxicity studies and the magnitude of test article-related changes is frequently expressed using quantitative and/or qualitative severity descriptors. Quantitative descriptors (ie, percent or fold change) are easily calculated to express numerical magnitude of a change but may not adequately convey biological relevance. A specific quantitative magnitude may be associated with vastly different levels of pathophysiologic relevance depending on several factors, including the nature of the endpoint, the animal species/strain, and the magnitude and direction of change. Qualitative descriptors (eg, minimal and mild) offer a succinct way to provide additional context to the pathophysiologic relevance but are more challenging to ascribe to a change. The assignment of qualitative descriptors often requires a subjective, comprehensive, and multifaceted approach using various factors in addition to numerical calculation. Because of the subjectivity involved, the qualitative severity descriptor assigned to a specific change may differ among clinical pathology endpoints, species/strain, contributing scientists, and studies/programs. Quantitative and qualitative severity descriptors may provide complementary information and may be used individually or in combination. This opinion piece primarily explains the process and discusses caveats and various factors taken into consideration by clinical pathologists while ascribing qualitative severity descriptors.","PeriodicalId":23113,"journal":{"name":"Toxicologic Pathology","volume":" ","pages":"561-570"},"PeriodicalIF":1.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uveodermatologic Syndrome in a Laboratory Beagle Dog: Histological and Immunohistochemical Features. 实验室比格犬葡萄膜皮肤病综合征：组织学和免疫组织化学特征。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-08-01 Epub Date: 2025-05-15 DOI: 10.1177/01926233251339116

Giulia Tosi, Elisavet Karamanavi, Zuhal Dincer, Michela Levi

引用次数: 0

Multiplexed siRNA Immunoassay Unveils Spatial and Quantitative Dimensions of siRNA Function, Abundance, and Localization In Vitro and In Vivo. 多重siRNA免疫分析揭示了siRNA在体外和体内功能、丰度和定位的空间和定量维度。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-08-01 Epub Date: 2025-01-19 DOI: 10.1177/01926233241311539

Michael Ly, Sandra Diaz-Garcia, Nathaniel Roscoe, Irina Ushach, Zhigang Hong, Monique França, Stephanie Schaffer, Tong-Yuan Yang, Mathieu Marella, Glenn Marsboom, Donna Klein, Tamar R Grossman, Vinicius Carreira, Michael Ollmann

{"title":"Multiplexed siRNA Immunoassay Unveils Spatial and Quantitative Dimensions of siRNA Function, Abundance, and Localization In Vitro and In Vivo.","authors":"Michael Ly, Sandra Diaz-Garcia, Nathaniel Roscoe, Irina Ushach, Zhigang Hong, Monique França, Stephanie Schaffer, Tong-Yuan Yang, Mathieu Marella, Glenn Marsboom, Donna Klein, Tamar R Grossman, Vinicius Carreira, Michael Ollmann","doi":"10.1177/01926233241311539","DOIUrl":"10.1177/01926233241311539","url":null,"abstract":"Small interfering RNAs (siRNAs) have been successfully used as therapeutics to silence disease-causing genes when conjugated to ligands or formulated in lipid nanoparticles to target relevant cell types for efficacy while sparing other cells for safety. To support the development of new methods for delivery of siRNA therapeutics, we developed and characterized a panel of antibodies generated against chemically modified nucleotides used in therapeutic siRNA molecules, identifying a monoclonal antibody that detects a broad range of siRNA representing distinct sequences and modification patterns. By integrating this anti-siRNA antibody with additional reagents, we created a multiplex siRNA immunoassay that simultaneously quantifies siRNA uptake, trafficking, and silencing activity. Using immunohistochemistry (IHC), we applied our method on tissues from mice treated with unconjugated, GalNAc-conjugated, or cholesterol-conjugated siRNAs and quantitatively assessed the biodistribution and activity of siRNAs in various organs. In addition, we used high-content imaging (HCI) and applied our multiplex siRNA immunoassay in tissue culture to enable simultaneous quantification of siRNA uptake, activity, and intracellular colocalization with endosome markers. These methods provide a robust platform for testing nucleic acid delivery methods in vitro and in vivo, allowing precise analysis and visualization of the pharmacokinetics and pharmacodynamics of siRNA therapeutics with cellular and subcellular resolution.","PeriodicalId":23113,"journal":{"name":"Toxicologic Pathology","volume":" ","pages":"536-547"},"PeriodicalIF":1.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital Pathology and Artificial Intelligence Applied to Nonclinical Toxicology Pathology-The Current State, Challenges, and Future Directions. 数字病理学和人工智能在非临床毒理学病理学中的应用——现状、挑战和未来方向。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-08-01 Epub Date: 2025-06-03 DOI: 10.1177/01926233251340622

Gabriele Pohlmeyer-Esch, Charles Halsey, Julie Boisclair, Sripad Ram, Sarah Kirschner-Kitz, Brian Knight, Pierre Moulin, Anna-Lena Frisk

{"title":"Digital Pathology and Artificial Intelligence Applied to Nonclinical Toxicology Pathology-The Current State, Challenges, and Future Directions.","authors":"Gabriele Pohlmeyer-Esch, Charles Halsey, Julie Boisclair, Sripad Ram, Sarah Kirschner-Kitz, Brian Knight, Pierre Moulin, Anna-Lena Frisk","doi":"10.1177/01926233251340622","DOIUrl":"10.1177/01926233251340622","url":null,"abstract":"Advancements in digital pathology and artificial intelligence (AI) have enormous transformative potential for nonclinical toxicologic pathology and are already changing the ways in which pathologists work. However, due to the rapid evolution of digital pathology and AI, the toxicologic pathology community would benefit from an update on these advancements, which can be used to aid drug development. Here we identify key articles published on the use of digital pathology and AI in the field and provide current regulatory statuses and guidelines. For digital pathology, we outline the requirements for equipment, validation processes, workflows, and archiving. Challenges to achieve system interoperability and to establish harmonization through Digital Imaging and Communications in Medicine compatibility are also discussed. For AI, we highlight considerations for model development, including the determination of ground truth, problems that may arise due to bias, and how the accuracy and precision of AI algorithms can be assessed. Finally, we discuss the challenges and potential for AI-assisted toxicologic pathology, picturing a future where technology and scientific expertise work hand-in-hand to improve the quality and efficiency of nonclinical drug safety evaluation. This publication is a deliverable of the European Innovative Medicines Initiative 2 Joint Undertaking, \"Bigpicture.\"","PeriodicalId":23113,"journal":{"name":"Toxicologic Pathology","volume":" ","pages":"516-535"},"PeriodicalIF":1.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep Learning Methodology for Quantification of Normal Pancreas Structures. 用于定量正常胰腺结构的深度学习方法。

IF 1.8 4区医学

Toxicologic Pathology Pub Date : 2025-08-01 Epub Date: 2025-06-16 DOI: 10.1177/01926233251341824

Zhiyong Xie, Stephane Thibault, Norimitsu Shirai, Yutian Zhan, Lindsay Tomlinson

引用次数: 0