The Open Pharmacology Journal最新文献_第2页

Editorial: Evaluating Impact of High-Cost Cancer Drugs at Regional Level: The Case of Veneto (Italy) 社论:在区域层面评估高成本抗癌药物的影响:威尼托(意大利)的案例

The Open Pharmacology Journal Pub Date : 2013-04-19 DOI: 10.2174/1874143601307010001

D. Gregori, D. Chiffi

引用次数: 0

Primary Sclerosing Cholangitis - Current Issues in Clinical Practice 原发性硬化性胆管炎-临床实践中的当前问题

The Open Pharmacology Journal Pub Date : 2012-06-17 DOI: 10.2174/1874143601206010045

L. Corless, H. Tsai

引用次数: 0

Intelligent Systems and the Systematization of Pharmacological Knowledge: Application of the In Vitro Retina Model 智能系统和药理学知识的系统化:体外视网膜模型的应用

The Open Pharmacology Journal Pub Date : 2012-05-21 DOI: 10.2174/1874143601206010034

V. M. F. Lima, José P. Castro, W. Hanke

引用次数: 1

Neuroprotection in Parkinson's Disease: A Systematic Review of thePreclinical Data 帕金森病的神经保护:临床前数据的系统回顾

The Open Pharmacology Journal Pub Date : 2012-05-09 DOI: 10.2174/1874143601206010012

H. Douna, B. Bavelaar, H. Pellikaan, B. Olivier, T. Pieters

{"title":"Neuroprotection in Parkinson's Disease: A Systematic Review of thePreclinical Data","authors":"H. Douna, B. Bavelaar, H. Pellikaan, B. Olivier, T. Pieters","doi":"10.2174/1874143601206010012","DOIUrl":"https://doi.org/10.2174/1874143601206010012","url":null,"abstract":"Aim: This study aimed to systematically review the preclinical data of neuroprotective agents for Parkinson's disease (PD) to support the translation of these compounds. Methods: The study consisted of two phases. In phase I, Pubmed and Scopus were systematically searched for neuroprotective agents for PD. In phase II, a systematic search was conducted for each substance identified in phase I. Articles were included if they used MPTP, 6-OHDA, rotenone or paraquat injury models. Results: Phase I led to the identification of 168 putative neuroprotective agents. Eventually ten compounds were included: melatonin, estrogen, nicotine, caffeine, riluzole, curcumin, coenzyme Q10, aspirin, EGCG and resveratrol. Phase II revealed 113 experimental studies and three reviews. Conclusion: This study clearly depicts the preclinical data of ten promising neuroprotective agents. While some of these compounds have already been tested in clinical use, none of them was studied in an appropriately designed trial to determine a neuroprotective effect. In expectation of qualitatively improved neuroprotection trials, the data from this study provide a firm foundation for future research.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73055661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Functional Role of Glycosylation in a Human IgG4 Antibody Assessed by Surface Plasmon Resonance Technology 用表面等离子体共振技术评价糖基化在人IgG4抗体中的功能作用

The Open Pharmacology Journal Pub Date : 2012-05-09 DOI: 10.2174/1874143601206010027

R. B. Wong, M. Zeng, an-Horng Lee, T. S. Raju, K. Cheng

{"title":"Functional Role of Glycosylation in a Human IgG4 Antibody Assessed by Surface Plasmon Resonance Technology","authors":"R. B. Wong, M. Zeng, an-Horng Lee, T. S. Raju, K. Cheng","doi":"10.2174/1874143601206010027","DOIUrl":"https://doi.org/10.2174/1874143601206010027","url":null,"abstract":"Fc glycosylation of immunoglobulins is necessary for antibody effector functions. These glycans of immunoglobulins are often referred as glycoforms and they can be heterogeneous due to the variations in glycosylation machinery present in the endoplastic reticulum (ER) and in the Golgi. In the absence of a generic culturing protocol that can render a consistent glycosylation pattern, monitoring glycoforms of monoclonal antibodies from cultured cells is becoming essential. Accordingly, quantification of glycosylated and deglycosylated heavy chains of an IgG4 monoclonal antibody was accomplished using an Agilent Bioanalyzer Lab-On-the-Chip electrophoresis system. In addition to the native antibody, completely deglycosylated antibody prepared by treating with PNGase F and a F(ab')2 fraction were evaluated for their antigen binding kinetics using Biacore surface Plasmon resonance (SPR). The equilibrium binding constants KD are found to be comparable at 1.81E-09M, 1.96E-09M, for the native and deglycosylated antibody, respectively, and 5.79E-10M for the F(ab')2. An in vitro biological activity employing a competition binding assay was also developed to demonstrate the role of the Fc glycan. The results confirm that for a neutralizing antibody therapeutic the biological activity of the native MAb-1 and deglycosylated antibody are comparable, thus indicating that the Fc glycan does not contribute to the antigen binding or the biological function. The kinetics and competitive assays performed on an SPR instrument are quick and reliable. Combined with the on-chip electrophoresis method they can be used as monitoring methods for process development and quality control.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"10 1","pages":"27-33"},"PeriodicalIF":0.0,"publicationDate":"2012-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78627087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

CEACAM1 is a Privileged Cell Surface Antigen to Design Novel ScFv Mediated-Immunotherapies of Melanoma, Lung Cancer and Other Typesof Tumors CEACAM1是一种特殊的细胞表面抗原，可用于设计新的ScFv介导的黑色素瘤、肺癌和其他类型肿瘤的免疫疗法

The Open Pharmacology Journal Pub Date : 2012-04-26 DOI: 10.2174/1874143601206010001

M. Cianfriglia, V. Fiori, S. Dominici, S. Zamboni, M. Flego, M. Dupuis, A. Ascione, Mara Gellini, A. Mallano, M. Magnani

{"title":"CEACAM1 is a Privileged Cell Surface Antigen to Design Novel ScFv Mediated-Immunotherapies of Melanoma, Lung Cancer and Other Typesof Tumors","authors":"M. Cianfriglia, V. Fiori, S. Dominici, S. Zamboni, M. Flego, M. Dupuis, A. Ascione, Mara Gellini, A. Mallano, M. Magnani","doi":"10.2174/1874143601206010001","DOIUrl":"https://doi.org/10.2174/1874143601206010001","url":null,"abstract":"Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a cell surface glycoprotein involved in intercellular binding, belonging to the immunoglobulin superfamily. It is involved in cell-cell recognition and modulates cellular processes that range from vascular angiogenesis to the regulation of insulin homeostasis and T-cell proliferation. Aberrant expression of CEACAM1 is often associated with progression and metastatic potential in melanoma, lung carcinoma and other types of tumor. Tumor-specific antigens such as CEACAM1 are ideal targets for cancer immunotherapy because they are over-expressed by the cancer cell and not on non-malignant tissues, minimizing the risk of autoimmune destruction. Many of the limitations of therapeutic use of rodent monoclonal antibodies (mAbs) can now be overcome by exploiting the use of recombinant antibody fragments and the advances in antibody engineering methods to improve tumor retention, reduce immunogenicity and modulate pharmacokinetics. In addition, a novel effective model of immunotherapeutic treatments of tumors includes antibody drug conjugates (ADCs) that combine specific mAbs and antibody fragments with cytotoxic drugs, proteins, enzymes, radionuclides and nanoparticles. This review aims to describe how these antibody engineering approaches can meet the challenges for generating new and effective antibody constructs for diagnosis and therapy of CEACAM1 expressing malignancies.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"40 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2012-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79795950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Attenuation of Drug-Induced Loss of Righting Reflex Response by Pretreatment with a Nitric Oxide Donor 一氧化氮供体预处理对药物性翻正反射反应丧失的抑制作用

The Open Pharmacology Journal Pub Date : 2011-11-23 DOI: 10.2174/1874143601105010001

Yuhua Wang, R. Quock, R. Keegan

引用次数: 0

Inhibition of Inflammatory Cytokine Secretion by Plant-Derived Compounds Inuviscolide and Tomentosin: The Role of NFκB and STAT1~!2010-02-10~!2010-06-28~!2010-08-12~! 植物源化合物菊内酯和绒毛蛋白对炎性细胞因子分泌的抑制作用:nf - κ b和STAT1的作用2010-02-10 2010-06-28 2010-08-12

The Open Pharmacology Journal Pub Date : 2010-08-12 DOI: 10.2174/1874143601004010036

Galya Abrham, S. Dovrat, H. Bessler, S. Grossman, U. Nir, M. Bergman

{"title":"Inhibition of Inflammatory Cytokine Secretion by Plant-Derived Compounds Inuviscolide and Tomentosin: The Role of NFκB and STAT1~!2010-02-10~!2010-06-28~!2010-08-12~!","authors":"Galya Abrham, S. Dovrat, H. Bessler, S. Grossman, U. Nir, M. Bergman","doi":"10.2174/1874143601004010036","DOIUrl":"https://doi.org/10.2174/1874143601004010036","url":null,"abstract":"The plant Inula viscosa has been shown to possess many important medicinal benefits, including anti-inflammatory, anti-oxidant, anti-bacterial, and anti-fungal activities, but the plant metabolites that mediate these effects and their mechanism of action are poorly understood. In a previous study, we demonstrated a reduced expression of the p65 subunit of nuclear factor kappa B (NFB) in melanoma cells treated with the purified sesquiterpene lactone compounds, Inuviscolide (Inv) and Tomentosin (Tom), extracted from Inula viscosa leaves. In this study, we tested the in- vitro effect of these purified compounds on the secretion of pro-inflammatory cytokines from human peripheral blood mononuclear cells (PBMCs) upon stimulation with lipopolysaccharide (LPS) or phorbol myristate acetate (PMA). Their possible mechanism of action was also studied. The results showed that both agents caused decreased production of IL-2, IL-1� , IFN� , and slightly increased secretion of TNF� , whereas secretion of IL-6 was not affected. The elevated levels of TNFdid not appear to affect the viability of human PBMCs. Western blot analysis revealed a reduction in the protein level of both the transcription factor component p65/RelA of nuclear factor-� B (NF￻ B) and the signal transducer and activator of transcription 1 (STAT1) through proteosomal degradation. However, no change was observed in the expression level of the nuclear factor-� B component, p50 (NF￻ B), or the signal transducer and activator of transcription 3 (STAT3). Taken together, our results indicate the possible future use of these agents as an anti-inflammatory treatment in cases where overstimulation of cytokine secretion is the basis for the pathological symptoms.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"41 1","pages":"36-44"},"PeriodicalIF":0.0,"publicationDate":"2010-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90950247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Stress-Induced Hyperthermia in Translational Stress Research 转化应激研究中的应激诱导热疗

The Open Pharmacology Journal Pub Date : 2010-06-02 DOI: 10.2174/1874143601004010030

C. Vinkers, R. Penning, M. Ebbens, J. Helhammer, J. Verster, C. Kalkman, B. Olivier

引用次数: 14

Stress-Induced Hyperthermia, the Serotonin System and Anxiety 应激性热疗，血清素系统和焦虑

The Open Pharmacology Journal Pub Date : 2010-06-02 DOI: 10.2174/1874143601004010015

C. Vinkers, B. Olivier, J. Bouwknecht, L. Groenink, J. Olivier

{"title":"Stress-Induced Hyperthermia, the Serotonin System and Anxiety","authors":"C. Vinkers, B. Olivier, J. Bouwknecht, L. Groenink, J. Olivier","doi":"10.2174/1874143601004010015","DOIUrl":"https://doi.org/10.2174/1874143601004010015","url":null,"abstract":"The serotonin (5-HT) system plays a key role in the pathophysiology of psychiatric disorders including mood and anxiety disorders. A role for serotonin in stress-related disorders is further supported by the fact that clinically effective treatments for these disorders alter serotonergic neurotransmission. The therapeutic potential of serotonergic pharmacological interventions has resulted in a variety of preclinical approaches to study the serotonin system. Of these, the stress-induced hyperthermia (SIH) paradigm has been extensively used to study the serotonin system at a preclinical level. The SIH response uses the transient rise in body temperature in response to a stressor which can be reduced using anxiolytic drugs including benzodiazepines, CRF receptor antagonists and serotonergic ligands. The present review aims to discuss the acute and chronic effects of 5-HT ligands on the SIH response. Also, the SIH response in genetically modified mice that lack or overexpress specific serotonergic receptor subtypes or the serotonin transporter will be summarized. 5-HT1A receptor ligands reduce the SIH response, whereas acute administration of other serotonergic drugs (including 5-HT1B, 5-HT2 and 5-HT3 modulators and SSRIs) generally does not influence the SIH response. Also, the SIH paradigm is generally insensitive to detect the anxiolytic effects of chronic serotonergic antidepressants in rodents, and serotonergic drugs that have been found to reduce the SIH response acutely do so irrespective of the healthy or pathological status of an individual.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86430185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8