The Journal of protozoology research最新文献_第3页

Preliminary evaluation of oligomannose-coated liposome vaccines against lethal protozoan infections in mice 低聚甘露糖包被脂质体疫苗抗小鼠致死性原生动物感染的初步评价

The Journal of protozoology research Pub Date : 2007-06-01 DOI: 10.32268/JPROTOZOOLRES.17.1_9

Noritaka Kuboki, W. Tiwananthagorn, H. Takagi, T. Nakayama, X. Xuan, N. Inoue, I. Igarashi, K. Tsujimura, Y. Ikehara, N. Kojima, N. Yokoyama

引用次数: 1

Transcriptional analyses of Trypanosoma brucei gambiense from infected mice and in vitro culture 感染小鼠及体外培养的布氏冈比亚锥虫转录分析

The Journal of protozoology research Pub Date : 2007-06-01 DOI: 10.32268/JPROTOZOOLRES.17.1_25

Noritaka Kuboki, Michael K. Kibe, O. Thekisoe, C. Sugimoto, N. Inoue

{"title":"Transcriptional analyses of Trypanosoma brucei gambiense from infected mice and in vitro culture","authors":"Noritaka Kuboki, Michael K. Kibe, O. Thekisoe, C. Sugimoto, N. Inoue","doi":"10.32268/JPROTOZOOLRES.17.1_25","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.17.1_25","url":null,"abstract":"ABSTRACT With the hypothesis that African trypanosomes could have in vivo specific genes for adaptation to host’s environment, the present study was conducted by using suppressive subtractive hybridization (SSH) technique to seek the highly expressed genes especially in host. A total of 328 clones from the in vivo SSH library and that of 160 clones from the in vitro SSH library were analyzed in order to determine their expression levels, but none of the above-mentioned genes showed differential expression. This indicates that no trypanosome genes could be differentially expressed either the in vivo or in vitro propagated trypanosomes. Alternatively, there might be limitation for detecting specifically expressed genes in African trypanosomes using this method, because of their polycistronic gene expression. Key Words : differential expression; infection; Trypanosoma brucei The development of axenic culture methods for both bloodstream form (BSF) and procyclic form (PCF) of African trypanosomes (Hirumi and Hirumi 1989; Hirumi and Hirumi 1991) has contributed to further investigation of African trypanosomes. However, this invention has given rise to a question of whether biological characteristics of","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"258 1","pages":"25-31"},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76297975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of scavengers for active oxygen species on photoresponse and photodynamic damages to a pigmented protozoan, Blepharisma 活性氧清除剂对色素原生动物ble法利赛马光响应和光动力损伤的影响

The Journal of protozoology research Pub Date : 2007-06-01 DOI: 10.32268/JPROTOZOOLRES.17.1_1

Keisuke Yamamoto, Y. Takada, A. Kida, T. Akematsu, H. Kotsuki*, T. Matsuoka

{"title":"Effects of scavengers for active oxygen species on photoresponse and photodynamic damages to a pigmented protozoan, Blepharisma","authors":"Keisuke Yamamoto, Y. Takada, A. Kida, T. Akematsu, H. Kotsuki*, T. Matsuoka","doi":"10.32268/JPROTOZOOLRES.17.1_1","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.17.1_1","url":null,"abstract":"In the absence of O2, the step-up photophobic response of Blepharisma japonicum is suppressed. To evaluate the possibility that reactivated oxygen species (ROS) produced by blepharismin photosensitization are involved in the primary transduction for the step-up photophobic response of B. japonicum, the effects of OH radical scavengers and the singlet oxygen quencher on the photophobic response were examined. The reagents used in the present assays hardly suppressed the step-up photophobic response, even though OH radical scavengers were rescued from the photodynamic killing of the cells. Alternatively, in the presence of an electron acceptor, the latency of the step-up photophobic response was prolonged. In addition, oxyblepharismin fluorescence was quenched slightly in the presence of free amino acids. These results support a hypothesis proposed by Lenci's research group, that electron transfer from the first excited singlet state of blepharismin (or oxyblepharismin) to associated proteins may be involved in the primary phototransduction for the step-up photophobic response.","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"106 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85540918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Inhibitory effects of Melia azedarach L. extracts on the growth of Trichomonas vaginalis : Ultrastructural changes of Trichomonas vaginalis treated by Melia azedarach L 苦楝提取物对阴道毛滴虫生长的抑制作用:苦楝处理后阴道毛滴虫的超微结构变化

The Journal of protozoology research Pub Date : 2007-06-01 DOI: 10.32268/JPROTOZOOLRES.17.1_16

Yun-Sik Lee, I. Chung, W. Choi, Yoo-Jung Cho, J. Chu, B. Min, E. Shin

{"title":"Inhibitory effects of Melia azedarach L. extracts on the growth of Trichomonas vaginalis : Ultrastructural changes of Trichomonas vaginalis treated by Melia azedarach L","authors":"Yun-Sik Lee, I. Chung, W. Choi, Yoo-Jung Cho, J. Chu, B. Min, E. Shin","doi":"10.32268/JPROTOZOOLRES.17.1_16","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.17.1_16","url":null,"abstract":"Meliae cortex (Melia azedarach Linne. var. japonica Makino, M. toosendan Siebold & Zucc) is one of the herbs used in oriental medicine for the treatment of various types of vaginitis. The present study was performed to examine the effects of Meliae cortex extracts on the growth and fine structure of Trichomonas vaginalis (T. vaginalis). Meliae cortex dose-dependently reduced the viability of T. vaginalis. In the electron microscopy study, we also observed the ultrastructural changes of T. vaginalis following the treatment with Meliae cortex. One to two hours after the addition of the extract of Meliae cortex, the movement of flagella and axostyle decreased, but the cell death did not occur. The fine structure of the cytoplasm was changed 30 min to 2 hour after the treatment of the extract of Meliae cortex. The number of polyribosome decreased, whereas the number of single ribosomes in the cytoplasm increased. These results indicate that Meliae cortex has the antiprotozoal effects on T. vaginalis cells through the inhibition of cell multiplication as well as the impairment of protein synthesis.","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"26 1","pages":"16-24"},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85017447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Evaluation of Alcian Blue 8GS and Pyronin B Stain for Rapid Wet Mount Preparation of SAF Preserved Specimen Containing Entamoeba histolytica and Giardia intestinalis 阿利新蓝8GS和Pyronin B染色快速湿Mount制备溶组织内阿米巴和肠贾第虫SAF保存标本的评价

The Journal of protozoology research Pub Date : 1998-08-01 DOI: 10.1016/S1383-5769(98)81129-X

Y. Maeno, H. Furukawa, Y. Kusuhara, T. Nisikawa, K. Nagase, Madoka Ito, T. Nakabayashi

引用次数: 0

Pararosaniline-induced akinetoplastic Trypanosoma evansi : formation and characterization 副花生苯胺诱导的动变性伊凡斯锥虫的形成和特征

The Journal of protozoology research Pub Date : 1995-01-01 DOI: 10.11501/3103914

Mary Rose Agnes Silva-Tahat

引用次数: 4