The Journal of protozoology research最新文献

Malaria infections among pregnant women attending antenatal clinic at Bududa hospital, eastern Uganda 乌干达东部Bududa医院产前门诊孕妇的疟疾感染情况

The Journal of protozoology research Pub Date : 2019-01-01 DOI: 10.32268/JPROTOZOOLRES.29.1-2_44

Kalyetsi Rogers, N. Gertrude, M. Enoch

引用次数: 1

Improvement of SYBR Green I−based fluorescence assay reading procedure for anti−babesial drugs screening in vitro 基于SYBR Green I -的体外抗巴贝虫药物筛选荧光检测方法的改进

The Journal of protozoology research Pub Date : 2019-01-01 DOI: 10.32268/JPROTOZOOLRES.29.1-2_26

T. Munkhjargal, G. Batiha, A. M. Beshbishy, M. Rizk, Azirwan Guswanto, T. Onikubo, T. Dickson, N. Yokoyama, I. Igarashi

引用次数: 1

Detection of Mycoplasma and Hepatozoon spp. in Philippine Dogs 菲律宾犬支原体和肝虫的检测

The Journal of protozoology research Pub Date : 2019-01-01 DOI: 10.32268/JPROTOZOOLRES.29.1-2_1

A. Ybañez, R. H. Ybañez, A. Estrera, Maxfrancis G. Talle, Mingming Liu, X. Xuan

引用次数: 1

Anti-piroplasmic potential of the methanolic Peganum harmala seeds and ethanolic Artemisia absinthium leaf extracts 乙醇性苦艾叶提取物和甲醇性苦艾种子抗肾上腺浆电位的研究

The Journal of protozoology research Pub Date : 2019-01-01 DOI: 10.32268/JPROTOZOOLRES.29.1-2_8

G. Batiha, A. M. Beshbishy, D. S. Tayebwa, O. Adeyemi, N. Yokoyama, I. Igarashi

{"title":"Anti-piroplasmic potential of the methanolic Peganum harmala seeds and ethanolic Artemisia absinthium leaf extracts","authors":"G. Batiha, A. M. Beshbishy, D. S. Tayebwa, O. Adeyemi, N. Yokoyama, I. Igarashi","doi":"10.32268/JPROTOZOOLRES.29.1-2_8","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.29.1-2_8","url":null,"abstract":"The available drugs against piroplasmosis are insufficient and faced with several challenges, such as drug-resistant parasites and toxicity to treated animals. Therefore, the discovery of new drug compounds is necessary for the effective control of babesiosis and theileriosis. Methanolic Peganum harmala seed extract (MPHSE) and ethanolic Artemisia absinthium leaf extract (EAALE) have several medicinal properties. In the present study, the growth-inhibition effects of MPHSE and EAALE were evaluated in vitro and in vivo. The half-maximal inhibitory concentration (IC50) values for MPHSE against Babesia bovis, B. bigemina, B. divergens, B. caballi, and Theileria equi were 24.9 ± 1.2, 77.1 ± 2.3, 61.1 ± 2.9, 80.8 ± 4.1, and 11.3 ± 2.1 μg/mL, respectively. EAALE exhibited IC50 values of 43.3 ± 3.1, 39.2 ± 2.7, 38.5 ±3.7, 50.3 ± 2.1, and 28.2 ± 2.1 μg/mL against B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively. The toxicity assay on Madin–Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3T3), and human foreskin fibroblast (HFF) cell lines showed that MPHSE affected the viability of MDBK, NIH/3T3, and HFF cell lines with half maximum effective concentration (EC50) values of 611.7± 10.9, 870 ± 22, and ˃1500 μg/mL, respectively, while EAALE exhibited EC50 values of 340.7 ± 8.5, 736.7 ± 9.3, and 1371.5 ± 17.3 μg/mL against MDBK, NIH/3T3, and HFF cell lines, respectively. In the in vivo experiment, MPHSE and EAALE oral treatments at 150 mg/kg inhibited the growth of Babesia microti in mice by 60% and 55.1%, respectively. These findings suggest that MPHSE and EAALE have the potential to be alternative remedies for treating piroplasmosis.","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"116 1","pages":"8-25"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73136079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Lepidosomes acquire fluorescence after encystation: Including additional notes of morphological events during encystation and reconsideration of the morphological features in the ciliate Colpoda cucullus 鳞状体在囊化后获得荧光:包括囊化过程中形态学事件的附加注释和对纤毛虫囊状体形态特征的重新考虑

The Journal of protozoology research Pub Date : 2019-01-01 DOI: 10.32268/JPROTOZOOLRES.29.1-2_51

Yoichiro Sogame, R. Saito, T. Sakai, Taiga Shimizu, Taiki Ono, R. Koizumi, Kaito Mizumachi

引用次数: 1

The effects of magnesium oxide nanoparticles on population growth of Paramecium caudatum. 氧化镁纳米颗粒对尾草履虫种群生长的影响。

The Journal of protozoology research Pub Date : 2018-01-01 DOI: 10.32268/JPROTOZOOLRES.28.1-2_1

Seyed Sajad Shahrokhi, M. Tabandeh, M. Kesmati

引用次数: 0

Chloroquine resistance status a decade after: Re-emergence of sensitive Plasmodium falciparum strains in malaria endemic and epidemic areas in Kenya 十年后的氯喹耐药状况:肯尼亚疟疾流行和流行地区敏感恶性疟原虫毒株再次出现

The Journal of protozoology research Pub Date : 2011-06-01 DOI: 10.32268/JPROTOZOOLRES.21.1_20

L. Wangai, S. Omar

{"title":"Chloroquine resistance status a decade after: Re-emergence of sensitive Plasmodium falciparum strains in malaria endemic and epidemic areas in Kenya","authors":"L. Wangai, S. Omar","doi":"10.32268/JPROTOZOOLRES.21.1_20","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.21.1_20","url":null,"abstract":"Development and spread of chloroquine (CQ) resistance led to its withdrawal in most malaria endemic countries. In Kenya, this occurred in 1998 when clinical efficacy dropped below 50%. Less than a decade after CQ was removed from routine use in Malawi, the drug has reversed to activity and is again effective for first-line treatment of uncomplicated malaria. There is a probability of a similar reversed activity in Kenya for more 10 years of its absence in uncomplicated Plasmodium falciparum malaria treatment. The present study was aimed at establishing the CQ resistance status in the country, 10 years after its withdrawal, by looking at high malaria transmission zone, Mbita, a malaria endemic area and some malaria epidemic areas of the Kenyan highlands. The prevalence of T76 and Y86 P. falciparum molecular markers for CQ resistance in Pfcrt and Pfmdr1 genes were investigated by PCR-RFLP and dot blot analysis in 64 samples collected in March to May 2007 in the endemic area and 38 samples collected in April to July the same year in the epidemics. The study shows that 67.3% of field isolates from the endemic site still harbor Y86 mutation in Pfmdr1 while 32.7% have the wild type allele N86 compared to the 94% and 6 % prevalence observed in Mwea, an endemic area, in 2004 (χ 2 =10.08, P=0.00015, 95% CI=2.085-27.8). In the epidemics 75% of field isolates from the epidemic sites still harbor Y86 mutation in Pfmdr1 while 25% have the wild type allele N86 compared to the 91.6% and 8.4% prevalence observed in an epidemic area in 1997 (χ 2 =1.585, P=0.208, 95% CI=0.701-19.176). From the study there is a significant change in the proportions of the resistant genotypes in the endemic areas while in the epidemics, there was also a noticeable shift though not significant. This therefore indicates a slow but steady re-emergence of P. falciparum CQ sensitive strains in the country. Though does not warrant the reintroduction of CQ for malaria treatment.","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"28 1","pages":"20-29"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87976529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

In vitro studies on the anti-trypanosomal effect of Jatropha gossypiifolia var gossypiifolia 麻疯树抗锥虫作用的体外研究

The Journal of protozoology research Pub Date : 2010-01-01 DOI: 10.32268/JPROTOZOOLRES.20.1_45

B. Shamaki, B. M. Agaie, P. O. Ajagbonna, A. Elsa, Y. A. Bashir, B. Iliyasu, A. A. Ebbo, A. Shehu, A. Sakuma

引用次数: 0

Differences in CD4+CD25+ regulatory T cell-depletion between Babesia microti and Babesia rodhaini infections in mice 微巴贝斯虫和罗德海巴贝斯虫感染小鼠CD4+CD25+调节性T细胞耗竭的差异

The Journal of protozoology research Pub Date : 2010-01-01 DOI: 10.32268/JPROTOZOOLRES.20.2_20

N. Yokoyama, Y. Ikehara, W. Tiwananthagorn, N. Ota, I. Igarashi, N. Kojima, O. Taguchi, K. Tsujimura

引用次数: 0

Molecular characterization and expression of a 47- kDa merozoite surface protein of Babesia gibsoni for serodiagnosis by enzyme-linked immunosorbent assay 酶联免疫吸附法检测巴贝斯虫血清诊断用47- kDa分裂子表面蛋白的分子特征和表达

The Journal of protozoology research Pub Date : 2010-01-01 DOI: 10.32268/JPROTOZOOLRES.20.1_59

G. Aboge, V. Batbaatar, Y. Goo, J. Yamagishi, Y. Nishikawa, F. Sunaga, K. Namikawa, I. Igarashi, K. Fujisaki, Hiroshi Suzuki, X. Xuan

引用次数: 4