发布求助
文献互助 智能选刊 最新文献

The Journal of protozoology最新文献

筛选
英文 中文
Value of an immunofluorescence assay for the detection of Pneumocystis carinii in Bronchoalveolar lavage. 免疫荧光法检测支气管肺泡灌洗液卡氏肺囊虫的价值。
The Journal of protozoology Pub Date : 1991-11-01
L Traore, P Roux, J L Poirot, C Chouaid, N el Helali, M Marteau-Miltgen, M Denis, F Balmy-Rapontchombo
{"title":"Value of an immunofluorescence assay for the detection of Pneumocystis carinii in Bronchoalveolar lavage.","authors":"L Traore,&nbsp;P Roux,&nbsp;J L Poirot,&nbsp;C Chouaid,&nbsp;N el Helali,&nbsp;M Marteau-Miltgen,&nbsp;M Denis,&nbsp;F Balmy-Rapontchombo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An indirect immunofluorescence monoclonal antibody assay was found to have higher sensitivity than usual stains for the detection of Pneumocystis carinii, particularly in bronchoalveolar lavage fluids in which there are only a few parasites, as in HIV-patients or in HIV+ patients with prophylaxis or treatment. For patients without any therapy, when different stains give conflicting results, decisions on therapeutic approaches to be used should consider the patient's clinical and biological status. Prospective studies are necessary to evaluate the predictive value of low parasitism in asymptomatic immunosuppressed patients.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Attempts at in vitro cultivation of Pneumocystis carinii from human bronchoalveolar lavage fluids without feeder cells. 人支气管肺泡灌洗液体外培养卡氏肺囊虫的尝试。
The Journal of protozoology Pub Date : 1991-11-01
B Le Marchand-Auffret, J Polianski, P Roux
{"title":"Attempts at in vitro cultivation of Pneumocystis carinii from human bronchoalveolar lavage fluids without feeder cells.","authors":"B Le Marchand-Auffret,&nbsp;J Polianski,&nbsp;P Roux","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We attempted to cultivate Pneumocystis carinii obtained from two bronchoalveolar lavage fluids of AIDS patients with P. carinii pneumonia, in a system wherein cysteine and 2-mercaptoethanol were substituted for the feeder cells. The presence of P. carinii cysts was monitored for 11 days under conditions of continuous culture. Moderate increase in cyst forms was observed until day 11. Further study with this system would be required to determine if the observed increase in cyst numbers is reproducible and whether the cyst form is a response to adverse in vitro conditions or is a manifestation of growth.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Videomicroscopic recording of Pneumocystis carinii motion. 卡氏肺囊虫运动的视频显微记录。
The Journal of protozoology Pub Date : 1991-11-01
A L Newsome, M M Durkin, M S Bartlett, J W Smith
{"title":"Videomicroscopic recording of Pneumocystis carinii motion.","authors":"A L Newsome,&nbsp;M M Durkin,&nbsp;M S Bartlett,&nbsp;J W Smith","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Videomicroscopy in combination with differential-contrast optics was used to study fresh preparations of Pneumocystis carinii from immunosuppressed rats. Certain spherical intracystic bodies appeared to move freely within the cyst wall. Flexing type movement was observed in intracystic ellipsoidal forms attached at a common point in the inner margin of the cyst wall. Greater movement was seen in non-attached thinner elongated forms. Possible extracellular trophic forms and movement were also identified. The movement of the morphological forms of P. carinii has been recorded in real time onto videotape. These initial observations suggest P. carinii is capable of movement and additional studies are under way to substantiate this possibility.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12980153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production and preparation of hyperimmune bovine colostrum for passive immunotherapy of cryptosporidiosis. 被动免疫治疗隐孢子虫病用高免疫牛初乳的生产与制备。
The Journal of protozoology Pub Date : 1991-11-01
R Fayer, M Tilley, S J Upton, A J Guidry, D W Thayer, M Hildreth, J Thomson
{"title":"Production and preparation of hyperimmune bovine colostrum for passive immunotherapy of cryptosporidiosis.","authors":"R Fayer,&nbsp;M Tilley,&nbsp;S J Upton,&nbsp;A J Guidry,&nbsp;D W Thayer,&nbsp;M Hildreth,&nbsp;J Thomson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pregnant cows were immunized to produce hyperimmune bovine colostrum (HBC) by intramuscular injection or intramammary infusion (TI) followed by 3 successive TI boosters with Cryptosporidium parvum (Cp) oocyst antigen mixed with Freund's (F) or Ribi (R) adjuvant. Control cows received no Cp. Colostrum from all cows was skimmed of butterfat and tested for specific anti-Cp immunoglobulin isotypes by ELISA. The HBC from Cp-F and Cp-R immunized cows had IgG1 titers exceeding 1:400,000 and 1:800,000, respectively. Some HBC from Cp-F immunized cows was freeze-dried to facilitate storage and some were irradiated at 42.5 kGy to kill potentially contaminating pathogens. Freeze-drying, but not irradiation, reduced IgG1 titers by only one dilution. Neither treatment affected Western blot banding patterns.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12980832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and isolation of Cryptosporidium parvum genes encoding microtubule and microfilament proteins. 小隐孢子虫微管和微丝蛋白编码基因的鉴定与分离。
The Journal of protozoology Pub Date : 1991-11-01
R G Nelson, K Kim, L Gooze, C Petersen, J Gut
{"title":"Identification and isolation of Cryptosporidium parvum genes encoding microtubule and microfilament proteins.","authors":"R G Nelson,&nbsp;K Kim,&nbsp;L Gooze,&nbsp;C Petersen,&nbsp;J Gut","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microtubules and microfilaments are highly conserved cytoskeletal polymers hypothesized to play essential biomechanical roles in the unusual gliding motility of Apicomplexan zoites and in their invasion of, and development within, host epithelial cells. We have identified and isolated Cryptosporidium parvum genes encoding the microtubule proteins alpha- and beta-tubulin and the microfilament protein actin by screening a lambda gt11 C. parvum genomic DNA library with degenerate oligonucleotide and heterologous cDNA hybridization probes respectively. The alpha- and beta-tubulin genes have been partially sequenced and the deduced peptide sequences show greatest homology with the tubulins of the related parasites, T. gondii and P. falciparum. The complete nucleic acid sequence of the actin gene predicts a 376 amino acid, 42 kDa protein having 85% sequence identity with the P. falciparum actin I and the human gamma-actin proteins. Each of these cytoskeletal protein genes was demonstrated to be of cryptosporidial origin by Southern analyses of C. parvum chromosomes fractionated by pulsed field gel electrophoresis; the cloned alpha- and beta-tubulin genes hybridized with chromosomes of ca. 1,200 and 1,500 kb respectively and the cloned actin gene also hybridized with a 1,200 kb chromosome.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12980836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of the beta-tubulin gene of Pneumocystis carinii. 卡氏肺囊虫β -微管蛋白基因的研究。
The Journal of protozoology Pub Date : 1991-11-01
T D Edlind, M S Bartlett, J W Smith
{"title":"Characterization of the beta-tubulin gene of Pneumocystis carinii.","authors":"T D Edlind,&nbsp;M S Bartlett,&nbsp;J W Smith","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To examine the potential of antimicrotubule drugs for treating Pneumocystis carinii infections, and to learn more about this unusual organism on a molecular level, we are studying its tubulin genes. A 0.3 kbp fragment of the P. carinii beta-tubulin gene was amplified by the polymerase chain reaction. Sequence analysis of this DNA revealed that P. carinii beta-tubulin is most closely related to those of the fungal molds. Consistent with these results, P. carinii growth in vitro was sensitive to the antifungal benzimidazoles benomyl and carbendazim.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression cloning of Pneumocystis carinii antigens. 卡氏肺囊虫抗原的表达克隆。
The Journal of protozoology Pub Date : 1991-11-01
A G Smulian, J R Stringer, M J Linke, P D Walzer
{"title":"Expression cloning of Pneumocystis carinii antigens.","authors":"A G Smulian,&nbsp;J R Stringer,&nbsp;M J Linke,&nbsp;P D Walzer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We undertook expression cloning of Pneumocystis carinii antigens to overcome the difficulties encountered in purification of these antigens. Using monoclonal antibodies to the P. carinii gp120 antigen and polyclonal rabbit antiserum to rat-derived P. carinii, we have isolated cDNA clones encoding immunoreactive moieties. A cDNA clone encoding the 3' portion of a 45-55 kDa antigen of rat-derived P. carinii, was the most abundant clone isolated. The peptide encoded by this cDNA has a novel sequence with a repeated motif rich in glutamic acid residues. Affinity-purified antibodies to this peptide reacted with the 45-55 kDa band of rat-derived P. carinii. The fusion protein was recognized by serum antibodies from rats with natural exposure to P. carinii. The production of this recombinant protein should allow more detailed studies of the host-parasite relationship of this important opportunistic infection.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of hyperimmune bovine colostrum raised against Cryptosporidium parvum on infection of guinea pigs by Cryptosporidium wrairi. 小隐孢子虫高免疫牛初乳对豚鼠隐孢子虫感染的影响。
The Journal of protozoology Pub Date : 1991-11-01
D Hoskins, C E Chrisp, M A Suckow, R Fayer
{"title":"Effect of hyperimmune bovine colostrum raised against Cryptosporidium parvum on infection of guinea pigs by Cryptosporidium wrairi.","authors":"D Hoskins,&nbsp;C E Chrisp,&nbsp;M A Suckow,&nbsp;R Fayer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Oocysts shedding was markedly reduced in guinea pigs inoculated intraintestinally with Cryptosporidium wrairi sporozoites that had been incubated with hyperimmune bovine colostrum raised to C. parvum when compared with shedding in guinea pigs inoculated with sporozoites incubated in either non-immune bovine colostrum or buffered saline. However oocyst shedding was apparently not reduced in guinea pigs inoculated by gavage with oocysts of C. wrairi and subsequently treated twice daily per os with hyperimmune bovine colostrum. Similarly, oocyst shedding was apparently not reduced by oral treatment with hyperimmune bovine colostrum when treatment was begun simultaneously with inoculation of C. wrairi oocysts.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of dexamethasone and dehydroepiandrosterone in immunosuppressed rats infected with Cryptosporidium parvum. 地塞米松和脱氢表雄酮对小隐孢子虫感染免疫抑制大鼠的影响。
The Journal of protozoology Pub Date : 1991-11-01
K R Rasmussen, E G Martin, M J Arrowood, M C Healey
{"title":"Effects of dexamethasone and dehydroepiandrosterone in immunosuppressed rats infected with Cryptosporidium parvum.","authors":"K R Rasmussen,&nbsp;E G Martin,&nbsp;M J Arrowood,&nbsp;M C Healey","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Treatment of rats immunosuppressed with dexamethasone and inoculated with Cryptosporidium parvum oocysts were treated with dehydroepiandrosterone. A significant reduction in cryptosporidial activity was observed as determined by oocyst shedding and colonization of host tissue by parasites. Dexamethasone treatment alone resulted in decreases in T-, B- and natural killer (NK) cell responses and antibody production that, with the exception of NK-cell activity, were all reversed after administration of dehydroepiandrosterone.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13002643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence. 聚合酶链反应检测卡氏肺囊虫DNA与直接镜检和免疫荧光法的比较。
The Journal of protozoology Pub Date : 1991-11-01
F Galan, J L Oliver, P Roux, J L Poirot, G Bereziat
{"title":"Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence.","authors":"F Galan,&nbsp;J L Oliver,&nbsp;P Roux,&nbsp;J L Poirot,&nbsp;G Bereziat","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The polymerase chain reaction (PCR) using published primers and probes has been compared to conventional stains and immunofluorescence for diagnosis of Pneumocystis carinii. We have screened 71 bronchoalveolar lavage (BAL) fluids from HIV-immunosuppressed patients. Of 34 samples negative by conventional stains and immunofluorescence, only one was positive by PCR. Thirty of 35 samples positive by conventional stains and immunofluorescence were also positive by PCR. One BAL sample, negative by conventional stains but positive by immunofluorescence, was negative by PCR. These data are discussed in relation to clinical and therapeutic conditions of the patients.</p>","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信