The Journal of Animal and Plant Sciences最新文献

{"title":"THE CO-ADMINISTRATION OF POIL-2 WITH TGEV INACTIVATED VACCINE ENHANCES IMMUNE RESPONSE OF PIGLETS TO TGEV","authors":"Hong Yu, Dongmei Gao, Fuhou Li, Dapeng Peng, B.B. Xia, Zhu Y. He, Wenjuan Xu, Haixia Li, Juan Du, Jun Zhao","doi":"10.36899/japs.2024.4.0776","DOIUrl":"https://doi.org/10.36899/japs.2024.4.0776","url":null,"abstract":"Porcine interleukin-2 (poIL-2) has not yet been demonstrated to be immune-enhancing against porcine transmissible gastroenteritis virus (TGEV) inactivated vaccine (IV), despite IL-2 having been proven to have immunological adjuvant effects for a variety of vaccinations. In this work, the impact of poIL-2 on TGEV IV in terms of immunological enhancement was investigated. Twenty four (24) SPF piglets were utilized and divided into six groups: PBS group, poIL-2 group, IV group, 10μg poIL-2+IV group, 50μg poIL-2 +IV group, and 250μg poIL-2+IV group. They received a second vaccine at 28 days point following the initial immunization. Serum and blood samples were obtained at various periods throughout the experiment. By using ELISA assay, neutralization assay, MTT assay, and flow cytometry assay, the TGEV-specific antibody expressions, neutralizing antibodies generations, interleukin-4 (IL-4), and interferon-gamma (IFN-γ) productions, peripheral blood mononuclear cells (PBMCs) proliferation response and lymphocyte phenotype subpopulations (CD3+, CD4+, and CD8+ immune cells) reflections were determined. The results showed that piglets inoculated with IV supplemented with poIL-2 significantly not only increased more piglet cellular immunity against TGEV by raising the degrees of IL-4, IFN-γ, Stimulation Index (SI), and the ratio of CD4+ /CD8+ cell subgroups, but also promoted more humoral immunity against TGEV by increasing levels of anti-TGEV specific antibodies and neutralizing antibodies (NAs) than those piglets inoculated with the TGEV IV alone. Additionally, the results suggested that porcine interleukin-2 (poIL-2) may improve pigs' immune responses in a dosage-dependent way.Our study revealed that poIL-2 had an immune-enhancing effect on the immunization of TGEV IV, and it possessed the potential to be applied as an immune-stimulating agent. Keywords: Inactivated vaccine (IV); adjuvant; porcine transmissible gastroenteritis virus (TGEV); porcine interleukin-2 (poIL-2); immune response.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"34 4","pages":"913-926"},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.thejaps.org.pk/docs/2024/04/09.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PREPARATION AND IN VITRO PERFORMANCE EVALUATION OF EGG YOLK IMMUNOGLOBULIN-LOADED SODIUM ALGINATE/CHITOSAN/SODIUM ALGINATE MICROCAPSULES FOR FEEDING","authors":"Yuanming Jinn, H.‐J. Lv, Chengdu Cui, Mingzhu Wang, Wu He, Lianhua Cui, Changguo Yan","doi":"10.36899/japs.2024.3.0756","DOIUrl":"https://doi.org/10.36899/japs.2024.3.0756","url":null,"abstract":"Egg yolk immunoglobulin (IgY) is valuable against intestinal infections, however, its activity will be affected by the gastrointestinal fluid of livestock and poultry and the storage environment. Therefore, we prepared IgY-loaded sodium alginate/chitosan/sodium alginate microcapsules (IgY-loaded SA/CS/SA MCs), aiming to improve the poor tolerance of IgY in the gastrointestinal tract of livestock and poultry as well as the instability of its storage performance, so as to improve the performance of its application. In this experiment, IgY was used as the core material, and sodium alginate and chitosan were used as the wall material, and the preparation was carried out by the extrusion method. The results showed that the encapsulation efficiency of the prepared IgY-loaded SA/CS/SA MCs was highest (94.84%) when the IgY concentration was 200 mg/mL, the CS concentration was 0.8%, and the CS solution pH value was 5.0 (P<0.05). After freeze-drying, the shape of the MCs in group T1 was more regular, with an average particle size of 2.159 mm and the highest compressive strength (P<0.05). The stability of the T1 group in simulated gastric fluid (pH 2.0) was significantly enhanced compared to that of the NC, T2, and T3 groups (P<0.05), and the T3 group showed significantly improved antibacterial properties and increased alkalinephosphatase content compared to the T1 and T2 groups (P<0.05). In addition, IgY-loaded SA/CS/SA MCs were stored at room temperature (23±2°C) for 8 weeks, and the IgY activity in the T1 group was maintained at >70% (P<0.05). Therefore, the IgY-loaded SA/CS/SA MCs enhanced the tolerance and storage stability of IgY in simulated gastrointestinal fluids and showed significant inhibition of E. coli and SG in vitro.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"34 3","pages":"696-707"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.thejaps.org.pk/docs/2024/03/16.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TEMPERATURE EFFECTS ON CORM GERMINATION AND FLOWERING OF CROCUS SATIVUS L. (SAFFRON)","authors":"","doi":"10.36899/japs.2024.1.0701","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0701","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"33 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139262057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DISTILLER’S DRIED GRAINS SUPPLEMENTED Wz3ITH ENZYME COCKTAIL AND YEAST AFFECT EGG QUALITY, REPRODUCTIVE PERFORMANCE AND BLOOD PROFILE OF BREEDING HENS","authors":"","doi":"10.36899/japs.2024.1.0714","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0714","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"13 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DYNAMICS OF SERUM BIOCHEMICAL ATTRIBUTES IN INDIGENOUS SIPLI SHEEP BREED KEPT UNDER INTENSIVE FARMING SYSTEM","authors":"","doi":"10.36899/japs.2024.1.0715","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0715","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"40 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139275689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF SINDH GOAT BREEDS PERFORMANCES FOR BODY MEASUREMENTS, BODY WEIGHT, PRODUCTIVE AND REPRODUCTIVE TRAITS","authors":"","doi":"10.36899/japs.2024.1.0713","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0713","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"30 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ENAMEL HYPOPLASIA AS A STRESS SIGNAL: UNVEILING PALEOENVIRONMENTAL STRESS EPISODES IN THE SIWALIK’S SUIDS OF PAKISTAN FROM THE MIDDLE MIOCENE TO EARLY PLEISTOCENE","authors":"","doi":"10.36899/japs.2024.1.0709","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0709","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"26 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139273158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR CHARACTERIZATION OF INFECTIOUS BURSAL DISEASE VIRUS ISOLATED FROM BROILER AND PULLET FLOCKS IN ALGERIA","authors":"","doi":"10.36899/japs.2024.1.0696","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0696","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MASTREVIRUS EFFECT ON METABOLIC AND BIOLOGICAL ACTIVITIES IN TOMATO LEAVES","authors":"","doi":"10.36899/japs.2024.1.0707","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0707","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"120 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"APHRODISIAC ACTIVITY OF TRIBULUS TERRESTRIS EXTRACT IMPROVES MALE REPRODUCTIVE SYSTEM PARAMETERS AND FERTILITY","authors":"","doi":"10.36899/japs.2024.1.0706","DOIUrl":"https://doi.org/10.36899/japs.2024.1.0706","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"119 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}