The Internet journal of microbiology最新文献_第4页

Enterococci: Emerging Drug Resistant Bacteria In Hospital Acquired Infections At Hospital Kuala Lumpur, Malaysia 肠球菌:新出现的耐药细菌在医院获得性感染在吉隆坡医院，马来西亚

The Internet journal of microbiology Pub Date : 2010-12-31 DOI: 10.5580/abb

R. Ibrahim, M. Mohamad, Mahmudur Rahman

{"title":"Enterococci: Emerging Drug Resistant Bacteria In Hospital Acquired Infections At Hospital Kuala Lumpur, Malaysia","authors":"R. Ibrahim, M. Mohamad, Mahmudur Rahman","doi":"10.5580/abb","DOIUrl":"https://doi.org/10.5580/abb","url":null,"abstract":"Enterococci are the most common pathogens in hospital acquired infections. Some of them are resistant to Vancomycin(VRE) and some are susceptible to Vancomycin (VSE). The present study was carried out to identify enterococci from clinical cases and to illustrate their clinical features and drug resistance characteristics. Antibiotics susceptibility of the identified bacteria was determined by disk diffusion method and E-test. Drug resistance properties were evaluated against ampicillin, gentamicin, vancomycin, teicoplanin and linezolid. Relating to clinical features, 244 cases of enterococci infected patients were identified at hospital Kuala Lumpur (HKL) Malaysia based on clinical information from the hospital. Of the patients 21% had history of urinary tract infections, 17.2% end stage renal disease, 12.2% sepsis, 8.4% malignancy, 12.2% had head injury and neurological problems, 4.2% diabetes mellitus and other clinical manifestations .In case of Vancomycin resistant enterococci infection, the clinical features of the patients were: end stage renal failure 3/6 (50%) and others with diabetes mellitus, interstitial lung disease and nephrotic syndrome. The patients of the enterococci infections were more prevalent in nephrology-urology unit (39%) and medical wards (including ICU, 23%) The findings would serve as an alert to the clinicians of the emergence of infections by enterococci and encourage implementation of appropriate infection control measures in order to curb further rise in prevalence.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78268161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

All Ceramic Veneers- A Case Reort 所有陶瓷贴面-一个案例报告

The Internet journal of microbiology Pub Date : 2010-12-31 DOI: 10.5580/213

Geeta Paul, R. Paul, M. Virdi, Priyank Banthia, Prerna Mathur

引用次数: 0

Midline Diastema Closure Following Post-Orthodontic Treatment Relapse Using Metal Free Restorations 正畸治疗复发后使用无金属修复体中线闭口

The Internet journal of microbiology Pub Date : 2010-12-31 DOI: 10.5580/23a0

R. Paul, Geeta Paul, R. Paul, T. Juneja, M. Virdi

引用次数: 0

Community-Acquired Mecithillin-Resistant Staphylococcus Aureus : A Case Report on Necrotizing Fasciitis And Deep Vein Thrombosis In Adolescent Boy 社区获得性耐甲氧西林金黄色葡萄球菌:青少年男孩坏死性筋膜炎及深静脉血栓1例报告

The Internet journal of microbiology Pub Date : 2010-12-31 DOI: 10.5580/cc5

Abdullah Alanazi, J. Alenazi, Suliman Alola, E. Banyan, M. Shalaan

{"title":"Community-Acquired Mecithillin-Resistant Staphylococcus Aureus : A Case Report on Necrotizing Fasciitis And Deep Vein Thrombosis In Adolescent Boy","authors":"Abdullah Alanazi, J. Alenazi, Suliman Alola, E. Banyan, M. Shalaan","doi":"10.5580/cc5","DOIUrl":"https://doi.org/10.5580/cc5","url":null,"abstract":"Staphylococcus aureus (S. Aureus) is the most common species of staphylococci to cause Staph infections. The infections caused by S. aureus were not difficult to manage. However, in recent past, S. aureus is transformed to its variant forms, including, Methicillin-resistant S. aureus (MRSA) by acquiring methicillin resistant gene (mec A gene). Previously, the MRSA was known for its origin from hospitals, but more recently, it is known to be acquired, from the communities too. Here we report the case presentation of a male adolescent with complicated fibular osteomyelitis, necrotizing fasciitis and deep vein thrombosis caused by Community acquired (CA) MRSA. The preliminary complaint of the patient was swelling in right foot and leg associated with high fever, pain, dyspnea and cough. The clinical presentation fit him as acute respiratory distress syndrome and hence antibiotics (ceftixone and vancomycin) were started. Doppler ultrasound revealed deep vein thrombosis (DVT). Analysis of blood culture showed the presence of MRSA. Hence rifampin and gentamicin were added and ceftrixon was discontinued. MRI revealed ostiomyelitis in the distal part of fibula. The bone infection was so severe that despite of using the three antibiotics, the patient remained febrile with increased leukocytes and neutrophills, the ankle redness became confined with malleollus on each side and the sepsis remained uncontrollable. Repeated MRI showed inflammatory findings, including cellulitis, pyogenic myositis, deep fasciitis and fibular periosteal reactions, suggestive of necrotizing fasciitis. Upon confirmation of fasciitis, fasciotomy with debridement and irrigations was done in multiple sessions and a high dose intravenous immunoglobulin was administered as an adjuvant therapy, resulting in reversal of the disease progression. In light of reports in the literature, we replaced antibiotics with immunoglobulins as a post surgery treatment, which worked well.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76988035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of inducible clindamycin resistance in Staphylococcus aureus and coagulase-negative staphylococci - a study from South India 南印度金黄色葡萄球菌和凝固酶阴性葡萄球菌诱导克林霉素耐药的检测

The Internet journal of microbiology Pub Date : 2010-12-31 DOI: 10.5580/1279

Shailesh Kumar, S. Umadevi, N. Joseph, A. Kali, J. Easow, S. Srirangaraj, G. Kandhakumari, R. Singh, Pravin M. V. Charles, S. Stephen

{"title":"Detection of inducible clindamycin resistance in Staphylococcus aureus and coagulase-negative staphylococci - a study from South India","authors":"Shailesh Kumar, S. Umadevi, N. Joseph, A. Kali, J. Easow, S. Srirangaraj, G. Kandhakumari, R. Singh, Pravin M. V. Charles, S. Stephen","doi":"10.5580/1279","DOIUrl":"https://doi.org/10.5580/1279","url":null,"abstract":"Background: Inducible clindamycin resistance is a major concern for the use of clindamycin to treat staphylococcal infections. Aims: To determine the prevalence of inducible clindamycin resistance in clinical isolates of Staphylococcus spp. and the susceptibility pattern of the isolates. Materials and Methods: A total of 300 isolates of Staphylococci spp. recovered from different clinical specimens were studied. All the Staphylococcus spp. were identified by conventional microbiological methods. Inducible clindamycin resistance was detected by double disk approximation test (Dtest).Results: Of the 300 isolates, 176 were identified as S. aureus, while 124 were coagulase negative staphylococci (CoNS). The rates of inducible clindamycin resistance in methicillin resistant S. aureus (MRSA), methicillin sensitive S. aureus (MSSA), methicillin resistant CoNS (MR-CoNS) and methicillin sensitive CoNS (MSCoNS) were 75.0%, 24%, 18.8% and 11.1%, respectively. The inducible clindamycin resistance was significantly more among MRSA compared to methicillin sensitive S. aureus (MSSA) (P value < 0.0001). Majority of the MRSA isolates were susceptible to clindamycin, vancomycin and linezolid, while most of them were resistant to erythromycin, gentamicin, ciprofloxacin, tetracycline and sulfamethoxazole-trimethoprim. Conclusion: In view of the significant in vitro inducible clindamycin resistance in Staphylococcus spp., we recommend that D test should be used as a mandatory method in microbiology laboratories to avoid misinterpretation of clindamycin result.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81085997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Biologic Effects Of Aloe Vera Gel 芦荟凝胶的生物效应

The Internet journal of microbiology Pub Date : 2010-12-31 DOI: 10.5580/c3f

N. Kathuria, Neelam Gupta, Manisha, R. Prasad, Nikita

引用次数: 14

Chikungunya confused with dengue in Malaysia: Clinical, serological and molecular perspective 基孔肯雅热在马来西亚与登革热混淆:临床、血清学和分子观点

The Internet journal of microbiology Pub Date : 2010-12-31 DOI: 10.5580/647

Umi Kalsom Ali, I. Isahak, M. Rahman

{"title":"Chikungunya confused with dengue in Malaysia: Clinical, serological and molecular perspective","authors":"Umi Kalsom Ali, I. Isahak, M. Rahman","doi":"10.5580/647","DOIUrl":"https://doi.org/10.5580/647","url":null,"abstract":"This study was to observe clinical, serolgical and molecular diagnosis of chikungunya viral fever patients and its comparison with dengue viral fever. For that 49 serologically negative dengue patients but clinically dengue like symptomms were included. Clinical investigation was carried out recording different profiles of hospitalized patients. Serology was performed using the Onsite® Chikungunya IgM Combo rapid test and molecular test RT-PCR was performed to detect the virus in the patients sera. It was observed that out of them 19 (38.7 %) were serologically confirmed chikungunya infection. Interestingly molecular detection of the patients sera did not show the presence chikungunya virus but detected dengue virus from 9 patients’s sera. Classical clinical features of chikungunya virus infected patients were recorded to differentiate chikungunya from dengue, which were fever, arthralgia, myalgia and rash. Detection of chikungunya IgM in these cohort means that this virus is circulating throughout the year although not as many as dengue. Laboratory confirmation is important to differentiate chikungunya fever from dengue fever. This study adds some information in local data and hopefully can help the clinicians to clinically and laboratory diagnosis and management of chikungunya infection in outbreak and non-outbreak setting. Serology was proven to be useful in confirming chikungunya infection.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88659614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Characterization of Bacillus cereus isolated from fermented cabbage and conventional optimization of extracellular protease production 发酵白菜蜡样芽孢杆菌的鉴定及胞外蛋白酶生产的常规优化

The Internet journal of microbiology Pub Date : 2009-12-31 DOI: 10.5580/e7d

C. E. Kotlar, Ponce A.G, R. Sansevero, S. Roura

引用次数: 5

Isolation Of Fungi And Bacteria From Housefly (Musca Domestica L.) Larvae 家蝇真菌和细菌的分离幼虫

The Internet journal of microbiology Pub Date : 2009-12-31 DOI: 10.5580/47d

Inocent C Omalu, V. Ayanwale, A. B. Ajalaruru, A. Z. Mohammed, J. Bala, V. Chukwuemeka

引用次数: 11

Purification and Characterization of Keratinase fromFeather Degrading Bacillus sp. 羽毛降解芽孢杆菌中角化酶的纯化及特性研究。

The Internet journal of microbiology Pub Date : 2009-12-31 DOI: 10.5580/159b

Jeevana Lakshmi. Peddu, C. Chitturi, V. Lakshmi

{"title":"Purification and Characterization of Keratinase fromFeather Degrading Bacillus sp.","authors":"Jeevana Lakshmi. Peddu, C. Chitturi, V. Lakshmi","doi":"10.5580/159b","DOIUrl":"https://doi.org/10.5580/159b","url":null,"abstract":"Microorganisms producing keratinases are gaining prominence in recycling of poultry waste feather. Keratinase producing Bacillus sp. Isolated, were subjected to strain improvement along with optimisation of parameters of fermentation. Scaled up enzyme was partially purified and characterized to evaluate its application potential. Keratinase isolated from the bacillus species exhibited good stability with > 90% activity being retained for one month at refrigerated conditions and 50% activity was observed even after two months. At room temperature, the enzyme was active for 24 � 48 hours with optimum temperature of activity being 55∞C. The activity range was between 28∞C - 55∞C and pH optima was 8.5 which matched with optima for production of the enzyme. ~38kDa keratinase produced by the isolates was categorized into subtilisin group of serine proteinase based on the sensitivity to protease inhibitors, Keratinase from the test isolates was only marginally affected by surfactants, organic solvents. A significant enhancement in activity was observed in presence of reducing agents indicating possible versatile application potential for this enzyme with high stability","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74876169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4