The hematology journal : the official journal of the European Haematology Association最新文献_第4页

Evolution of microbial safety. 微生物安全性的演变。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200426

John A J Barbara

引用次数: 2

Antiphospholipid syndrome. Antiphospholipid综合症。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200412

Jef Arnout, Milosz Jankowski

引用次数: 0

G-CSF induced progenitor mobilization in mice with PIGA- blood cells. G-CSF诱导PIGA-血细胞小鼠祖细胞动员。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200383

Bing Han, Jacqueline Unsinger, Fulu Liu, Dan C Link, Monica Bessler

{"title":"G-CSF induced progenitor mobilization in mice with PIGA- blood cells.","authors":"Bing Han, Jacqueline Unsinger, Fulu Liu, Dan C Link, Monica Bessler","doi":"10.1038/sj.thj.6200383","DOIUrl":"https://doi.org/10.1038/sj.thj.6200383","url":null,"abstract":"Objective: In patients with paroxysmal nocturnal hemoglobinuria (PNH) a proportion of blood cells are deficient in glycosyl phosphatidylinositol (GPI) anchored proteins due to a mutation in the PIGA gene. Previous studies showed that in PNH the majority of circulating early progenitor cells were normal but after G-CSF were mainly, of the PNH phenotype. This suggested that GPI-linked proteins contribute to the regulation of progenitor trafficking from bone marrow to peripheral blood.Methods: To test this hypothesis we studied progenitor cells in bone marrow, spleen, and peripheral blood in response to G-CSF in mice genetically engineered to have a proportion of blood cells deficient in GPI-linked proteins (LF mice).Results: In contrast to humans, LF and wild-type mice have comparable numbers of progenitor cells in bone marrow, spleen, and peripheral blood. Similarly, in LF mice the proportion of PIGA- progenitor cells in peripheral blood corresponds the proportion of PIGA- progenitor cells measured in bone marrow and spleen. After G-CSF the number of circulating progenitors significantly increased but the proportion of PIGA- cells remained the same in peripheral blood,bone marrow, and spleen.Conclusions: Our data indicate that under basal laboratory conditions the lack of GPI-linked protein does not cause a retention of progenitor cells in the bone marrow. This implies that the preferential circulation of normal progenitor cells in patients with PNH requires an additional component that most likely is provided by the altered microenvironment of the underlying bone marrow failure.","PeriodicalId":22486,"journal":{"name":"The hematology journal : the official journal of the European Haematology Association","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40894516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Chronic hepatitis C associated with Coombs-positive hemolytic anemia. 慢性丙型肝炎与库姆斯阳性溶血性贫血相关。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200400

Ihab I Elhajj, Ala' I Sharara, Ali T Taher

引用次数: 15

Steady-state plasma lactoferrin levels in relation to infections and complications of sickle cell disease. 稳态血浆乳铁蛋白水平与镰状细胞病感染和并发症的关系

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200372

Iheanyi Okpala, Cynthia C Ugochukwu, Adel Tawil, Rachael Greaves

引用次数: 0

Primary central nervous system lymphoma (PCNSL) in immunocompetent adults: analysis of a retrospective series of patients treated using idarubicin-containing regimen and radiotherapy. 免疫功能正常成人原发性中枢神经系统淋巴瘤(PCNSL):回顾性分析一系列使用含伊达柔比星方案和放疗治疗的患者。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200405

Maria Luigia Vigliotti, Matteo Dell'Olio, Antonio La Sala, Giampiero Romano, Alfredo Tartarone, Giuseppe Mele, Clelia Musto, Angelo Michele Carella, Nicola Di Renzo

引用次数: 2

Second autologous transplantation after failure of a first autologous transplant in 18 patients with non-Hodgkin's lymphoma. 18例非霍奇金淋巴瘤患者首次自体移植失败后的第二次自体移植。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200545

Christelle Lenain, Charles Dumontet, Therese Gargi, Catherine Chassagne, Francoise Berger, David Perol, Maud Garnier, Bertrand Coiffier, Jean Yves Blay

{"title":"Second autologous transplantation after failure of a first autologous transplant in 18 patients with non-Hodgkin's lymphoma.","authors":"Christelle Lenain, Charles Dumontet, Therese Gargi, Catherine Chassagne, Francoise Berger, David Perol, Maud Garnier, Bertrand Coiffier, Jean Yves Blay","doi":"10.1038/sj.thj.6200545","DOIUrl":"https://doi.org/10.1038/sj.thj.6200545","url":null,"abstract":"High-dose chemotherapy and autologous marrow or peripheral stem cell support offers the best chance of cure in some subgroups of patients with non-Hodgkin's lymphoma (NHL). Less is known about the role of a second course of myeloablative chemotherapy in patients who relapse after a first autologous transplant. The aim of this retrospective study was to evaluate the disease outcome, morbidity and mortality associated with second autologous transplantation in patients with NHL. Between 1985 and 2001, 225 patients who had received autologous transplantation for NHL in two institutions in Lyon relapsed. Of these 225 patients 18 underwent a second autologous transplantation. The median age at second transplant was 41 years. There were six indolent lymphomas and 12 aggressive lymphomas. The median follow-up from the second transplant was 42 months. The OS rate at 2 and 5 years were 58 and 27%, respectively. The PFS rate at 2 and 5 years was 36%. Five patients are alive without disease 20 to 100 months after the second transplant. Seven patients died of disease recurrence. Four (22%) toxic deaths occurred: one of pulmonary fibrosis, one of fungal infection and cardiac failure and two of acute leukaemia. A minority of patients with NHL recurrence after a first transplant can be cured by a second course of myeloablative chemotherapy at the cost however of high-risk toxic death.","PeriodicalId":22486,"journal":{"name":"The hematology journal : the official journal of the European Haematology Association","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/sj.thj.6200545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24759833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Targeting PML/RARalpha transcript with DNAzymes results in reduction of proliferation and induction of apoptosis in APL cells. DNAzymes靶向PML/ rar α转录物可降低APL细胞的增殖并诱导凋亡。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200535

Majid Kabuli, John Ahman Liu Yin, Khalid Tobal

{"title":"Targeting PML/RARalpha transcript with DNAzymes results in reduction of proliferation and induction of apoptosis in APL cells.","authors":"Majid Kabuli, John Ahman Liu Yin, Khalid Tobal","doi":"10.1038/sj.thj.6200535","DOIUrl":"https://doi.org/10.1038/sj.thj.6200535","url":null,"abstract":"DNAzymes are nucleic acid enzymes that can recognise specific RNA substrate via Watson-Crick base pairing and cleave it with multiple turnovers. We have designed and examined the effects of DNAzymes targeting the PML/RARalpha fusion gene in acute promyelocytic leukaemia (APL). The DNAzymes (DZ1 and DZ3) were designed to cleave the PML/RARalpha transcript at the GC nucleotides at the fusion point and three nucleotides upstream of that respectively. Disabled DNAzymes were synthesised and used as controls. Cell-free cleavage reactions were performed on total RNA from NB4 cell line and PML/RARalpha and RARalpha-amplified RNA fragments (aRNA). Postcleavage examination showed that DZ1 and DZ3 cleave PML/RARalpha efficiently and specifically. NB4 APL cells transfected with DZ1 or DZ3 showed a significant suppression of PML/RARalpha protein expression. These DNAzymes also inhibited the proliferation of NB4 cells, reduced the viability rate, and induced apoptosis in these cells. The disabled DNAzymes showed no effect on NB4 cells. The two DNAzymes did not produce any significant effect on K562 cells, which were used as control cells. DNAzymes are more resistant to serum than ribozymes. These data show that targeting the PML/RARalpha fusion gene with DNAzymes can induce apoptosis in APL cells and may have a role in the treatment of APL. They also show DNAzymes are promising tools for targeting specific genes in leukaemia.","PeriodicalId":22486,"journal":{"name":"The hematology journal : the official journal of the European Haematology Association","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/sj.thj.6200535","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24759836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Unexpectedly high but still asymptomatic iron overload in a patient with pyruvate kinase deficiency. 丙酮酸激酶缺乏症患者异常高但仍无症状的铁超载。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200556

Frank D Andersen, Francesco d'Amore, Finn Cilius Nielsen, Wouter van Solinge, Finn Jensen, Peter D Jensen

引用次数: 10

In malignant myeloid cells expression of Daxx downregulates expression of p53 and of the inhibitors of apoptosis proteins. 在恶性髓细胞中，Daxx的表达下调p53和凋亡抑制蛋白的表达。

The hematology journal : the official journal of the European Haematology Association Pub Date : 2004-01-01 DOI: 10.1038/sj.thj.6200547

Simone Boehrer, Daniel Nowak, Simone Schaaf, Marion Bergmann, Angela Brieger, Dieter Hoelzer, Paris S Mitrou, Eckhart Weidmann, Kai Uwe Chow

{"title":"In malignant myeloid cells expression of Daxx downregulates expression of p53 and of the inhibitors of apoptosis proteins.","authors":"Simone Boehrer, Daniel Nowak, Simone Schaaf, Marion Bergmann, Angela Brieger, Dieter Hoelzer, Paris S Mitrou, Eckhart Weidmann, Kai Uwe Chow","doi":"10.1038/sj.thj.6200547","DOIUrl":"https://doi.org/10.1038/sj.thj.6200547","url":null,"abstract":"The role of Daxx, in particular its ability to promote or hinder proliferation, still remains controversial. In order to elucidate the functional relevance of Daxx in malignant myelocytes, the erythroleukemia cell line HEL was stably transfected with a Daxx-expressing vector or with the respective Daxx-negative control vector. Assessing the molecular consequences of ectopic Daxx-expression, we present evidence that Daxx downregulates p53. Moreover, we demonstrate that Daxx overexpressing myelocytes downregulate the proapoptotic Bcl-2 family member Bax, while expression of antiapoptotic Bcl-2 is not influenced. Furthermore, expression of Daxx diminishes expression levels of the initiator-procaspase-8 and -10, and the executioner procaspase-7, whereas the procaspase-3, -6 and -9 remain unaltered. The altered protein levels of the caspases in Daxx overexpressing myelocytes are accompanied by a decrease of expression levels of the inhibitor of apoptosis proteins (IAPs) cIAP-1, -2 and survivin. Despite the described impact of Daxx expression on major molecules of the apoptotic cascade, expression of Daxx in neoplastic myelocytes does not impact on the rate of proliferation. Upon a proapoptotic stimulus such as serum withdrawal Daxx is unable to maintain its influence on expression levels of p53, Bax, IAPs and the procaspase-8, -10 and -7.","PeriodicalId":22486,"journal":{"name":"The hematology journal : the official journal of the European Haematology Association","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1038/sj.thj.6200547","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24836685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3