{"title":"Pollen morphology in the tribe Nigelleae (Ranunculaceae): a worldwide palynological investigation into the species","authors":"Serap Işık, E. Dönmez, Z. U. Aydın, A. Dönmez","doi":"10.24425/ABCSB.2019.127738","DOIUrl":"https://doi.org/10.24425/ABCSB.2019.127738","url":null,"abstract":"Plant tissue culture techniques have become an integral part of progress in plant science research due to the opportunity offered for close study of detailed plant development with applications in food production through crop improvement, secondary metabolites production and conservation of species. Because the techniques involve growing plants under controlled conditions different from their natural outdoor environment, the plants need adjustments in physiology, anatomy and metabolism for successful in vitro propagation. Therefore, the protocol has to be optimized for a given species or genotype due to the variability in physiological and growth requirement. Developing the protocol is hampered by several physiological and developmental aberrations in the anatomy and physiology of the plantlets, attributed to in vitro culture conditions of high humidity, low light levels and heteroor mixotrophic conditions. Some of the culture-induced anomalies become genetic, and the phenotype is inherited by clonal progenies while others are temporary and can be corrected at a later stage of protocol development through changes in anatomy, physiology and metabolism. The success of protocols relies on the transfer of plantlets to field conditions which has been achieved with many species through stages of acclimatization, while with others it remains a challenging task. This review discusses various adjustments in nutrition, physiology and anatomy of micro-propagated plants and field grown ones, as well as anomalies induced by the in vitro culture conditions.","PeriodicalId":224123,"journal":{"name":"Acta Biologica Cracoviensia s. Botanica","volume":"13 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128855694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Factors influencing the efficiency of wheat anther culture","authors":"C. Lantos, J. Pauk","doi":"10.24425/abcsb.2020.131671","DOIUrl":"https://doi.org/10.24425/abcsb.2020.131671","url":null,"abstract":"Using in vitro androgenesis serves as a unique opportunity to produce doubled haploid (DH) plants in many species. More benefits of this biological phenomenon have kept these methods in the focus of fundamental research and crop breeding for decades. In common wheat ( Triticum aestivum L.), in vitro anther culture is one of the most frequently applied DH plant production methods. The efficiency of in vitro wheat anther culture is influenced by many factors, such as the genotype, growing conditions, collection time, pre-treatments, and compositions of media and culture conditions. According to some critical review, the genotype dependency, low efficiency and albinism are mentioned as limitations of application of the anther culture method. However, some research groups have made significant efforts to diminish the effects of these bottlenecks. Due to the improvements, a well-established in vitro anther culture method can be an efficient tool in modern wheat breeding programs.","PeriodicalId":224123,"journal":{"name":"Acta Biologica Cracoviensia s. Botanica","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122805180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Myśków, I. Czyczyło-Mysza, Sandra Sokołowska, S. Stojałowski
{"title":"QTL analysis of moderate drought response in a rye Recombinant Inbred Line (RIL) population shows co-localization of QTLs for morphological and physiological traits","authors":"B. Myśków, I. Czyczyło-Mysza, Sandra Sokołowska, S. Stojałowski","doi":"10.24425/118050","DOIUrl":"https://doi.org/10.24425/118050","url":null,"abstract":"The study aimed to identify the loci responsible for some morphological traits, induced by drought in a recombinant inbred line (RIL) population of rye and to find out their connections with loci for other traits, using high density, consensus, genetic map. Twenty-five QTLs engaged in the response to drought (drought index, DI) of four morphological traits were detected. Thirteen QTLs induced by drought stress co-localized with QTLs for morphology, leaf rolling, pre-harvest sprouting (PHS), alpha-amylase activity and heading earliness. XrPt507374 from 3R was found to be the most validated marker, as the nearest one to the LOD peak of QTLs for five different traits: TGW-DI, plant height, spike length, spike compactness and PHS. Three genes were considered putative candidate genes for drought response on the basis of sequence alignment of DArTs linked to QTLs from 5R: DEAD-box ATP-dependent RNA helicase, thaumatin-like protein precursor (TLP) and transcription initiation factor TFIID.","PeriodicalId":224123,"journal":{"name":"Acta Biologica Cracoviensia s. Botanica","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127508592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Budzianowska, M. Kikowska, M. Małkiewicz, Inga Karolak, J. Budzianowski
{"title":"Phenylethanoid Glycosides in Plantago media L. Organs Obtained in In Vitro Cultures","authors":"A. Budzianowska, M. Kikowska, M. Małkiewicz, Inga Karolak, J. Budzianowski","doi":"10.24425/118060","DOIUrl":"https://doi.org/10.24425/118060","url":null,"abstract":"Plant tissue culture techniques have become an integral part of progress in plant science research due to the opportunity offered for close study of detailed plant development with applications in food production through crop improvement, secondary metabolites production and conservation of species. Because the techniques involve growing plants under controlled conditions different from their natural outdoor environment, the plants need adjustments in physiology, anatomy and metabolism for successful in vitro propagation. Therefore, the protocol has to be optimized for a given species or genotype due to the variability in physiological and growth requirement. Developing the protocol is hampered by several physiological and developmental aberrations in the anatomy and physiology of the plantlets, attributed to in vitro culture conditions of high humidity, low light levels and heteroor mixotrophic conditions. Some of the culture-induced anomalies become genetic, and the phenotype is inherited by clonal progenies while others are temporary and can be corrected at a later stage of protocol development through changes in anatomy, physiology and metabolism. The success of protocols relies on the transfer of plantlets to field conditions which has been achieved with many species through stages of acclimatization, while with others it remains a challenging task. This review discusses various adjustments in nutrition, physiology and anatomy of micro-propagated plants and field grown ones, as well as anomalies induced by the in vitro culture conditions.","PeriodicalId":224123,"journal":{"name":"Acta Biologica Cracoviensia s. Botanica","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127014171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Population genetic structure of Iris pumila L. in Ukraine: effects of habitat fragmentation","authors":"","doi":"10.24425/abcsb.2020.131665","DOIUrl":"https://doi.org/10.24425/abcsb.2020.131665","url":null,"abstract":"","PeriodicalId":224123,"journal":{"name":"Acta Biologica Cracoviensia s. Botanica","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114102867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic diversity and differentiation of coexisting populations of Quercus robur L.and Q. petraea (Matt.) Liebl.","authors":"Elżbieta Sandurska, B. Ulaszewski, J. Burczyk","doi":"10.24425/abcsb.2019.127739","DOIUrl":"https://doi.org/10.24425/abcsb.2019.127739","url":null,"abstract":"Pedunculate and sessile oaks (Quercus robur L.; Q. petraea [Matt] Liebl.) often coexist in mixed forest stands. However, species-specific investigations and forest management actions in such populations require reliable methods of identification of the species status of individuals. We investigated genetic diversity and species differentiation of adult and naturally established seedling cohorts in a mixed forest stand composed of Q. robur and Q. petraea, located in the Jamy Nature Reserve in north-central Poland. Using nineteen nuclear microsatellite loci and a model-based clustering approach as a tool for species delineation, we efficiently identified 105 and 60 adults, as well as 191 and 456 seedlings of pedunculate and sessile oaks, respectively. While the adult trees of both species were randomly distributed throughout the sample plot, the seedlings demonstrated significant spatial clustering, which was particularly evident for Q. petraea. The two oak species exhibited similar levels of genetic diversity in adult and offspring cohorts. Inbreeding was found to be low and significant only at the stage of seedlings. The estimates of effective population size were higher for Q. robur than Q. petraea, despite the overall greater reproductive success of the later one. There was a significant level of differentiation between the studied oak species, as measured by Fst coefficient (0.084 – adults; 0.099 – seedlings). The results on genetic diversity and species differentiation obtained in the studied indigenous near-natural stand of Q. robur and Q. petraea could be considered as a reference for other population genetic studies of oaks.","PeriodicalId":224123,"journal":{"name":"Acta Biologica Cracoviensia s. Botanica","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124647845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro polyploidiization of Ajuga reptans L. using oryzalin","authors":"M. Švécarová, B. Navrátilová, V. Ondřej","doi":"10.24425/118057","DOIUrl":"https://doi.org/10.24425/118057","url":null,"abstract":"","PeriodicalId":224123,"journal":{"name":"Acta Biologica Cracoviensia s. Botanica","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130347505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}