THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)最新文献_第10页

Impact Action of Some Volatile Oils and Antibiotics on GIT-Pathogenic Bacteria 一些挥发油和抗生素对git致病菌的影响作用

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2019-01-01 DOI: 10.5455/EGYJEBB.20190111081336

Gouda T. M. Dawoud, Mohamed E. A.Dawoud, Yousef E. Abdel Mawgood

{"title":"Impact Action of Some Volatile Oils and Antibiotics on GIT-Pathogenic Bacteria","authors":"Gouda T. M. Dawoud, Mohamed E. A.Dawoud, Yousef E. Abdel Mawgood","doi":"10.5455/EGYJEBB.20190111081336","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20190111081336","url":null,"abstract":"ABSTRACT Susceptibility of some gastrointestinal tract (GIT) bacterial-pathogens to combinations of MRSA-growth inhibiting antibiotics (MGIA), MRSA growth non-inhibiting antibiotics (MGNIA) and some plant extracts, separately or in groups were investigated. The results revealed that combinations from MGNIA (Gentamycin with Co trimoxazole), MGIA (Vancomycin with Ciprofloxacin) and MGNIA with MGIA (Gentamycin with Imipenem) were determinative for the growth of most tested GIT-pathogens. On the other hand, aerial shoots of Artimesia monosperma L (Am), Ocimum basilicum L (Ob), Origanum majorana L (Om), Salvia officinalis L (So) and Pelargonium graveolens (Pg) were applied collectively in a mixture (PM) and yield the most lethal effect on the tested pathogens (13.2 IZD). Further total plant mixture (TPM) Foeniculum vulgare (Fv) seed mixture (SM) with PM increased the determinative impact on pathogens growth and resulted in more susceptibility (17.3mm IZD). GC/MC analyses have shown that the major essential oils compounds of TM were β -Pinene 55 %, from (Am), Linalool 46%, Methyl chavicol 14.2% and 1, 8- cineole 12.3% from (Ob), terpinen-4-ol 31% and γ-Terpinene 14% from (Om), camphor 26.4 %, 1, 8- cineole 18% and α-thujone 14% from (So). Citronellol 40% from (Pg), Trans-anethole 66% and 1, 8- cineole12% from (Fv). Moreover, combination of TPM with Gentamicin and Imipenem (TPMGI) had a further upmost synergistic effect (25 mm IZD). The mode of action for TPMGI mixture ingredients may be resulted from the disruption of bacterial cell membrane, blocking of protein synthesis and out diffusion of cellular components.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79765707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dead biomass of thermophilic Aspergillus fumigatus for Congo red biosorption 嗜热烟曲霉对刚果红生物吸附的死生物量

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2019-01-01 DOI: 10.5455/EGYJEBB.20181206084342

T. M. Abdel-Ghany, M. Abboud, Mohamed M. Alawlaqi, A. M. Shater

引用次数: 10

Evaluating the efficiency of DNA Barcode rbcL for detection of genetic relationships between four Moringa spp. genotypes DNA条形码rbcL检测辣木四种基因型亲缘关系的有效性评价

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2019-01-01 DOI: 10.5455/egyjebb.20190722115434

Walaa A. Rayan

{"title":"Evaluating the efficiency of DNA Barcode rbcL for detection of genetic relationships between four Moringa spp. genotypes","authors":"Walaa A. Rayan","doi":"10.5455/egyjebb.20190722115434","DOIUrl":"https://doi.org/10.5455/egyjebb.20190722115434","url":null,"abstract":"Alterations in the DNA sequences are very useful for the unique markers development, which could be utilized as a barcoding of DNA for different plants. The barcoding of DNA is used for identification of living organisms and provides extra and integral support to identify the morpho-species, because it characterized by a reproducible, rapid and economic tool. This study was conducted to analyze the phylogenetic relationships between two species of the genus Moringa; M. oleifera and M. Peregrina, using DNA barcodes. In Egypt only two Moringa species (M. oleifera and M. Peregrina) were cultivated. These two species (four Moringa genotypes) were commonly used in the traditional medicine. To authenticate the different genotypes, rbcL regions were evaluated. DNA barcode regions were amplified using universal primers. The length of rbcL barcoding region varied between 638 bp in M. oleifera (II) collected from El-Suis governorate (Egypt) to 663 bp in M. peregrina from Giza (III), with a mean of 649.75 bp. In all investigated species, the percentage of GC content found to be nearly 44.01% and the span aligned sequences around 638-663 nucleotides. The number of variable sites within the sequences of the four Moringa genotypes was found to be 46 sites (11 transitions, 15 transversions and 20 indel). A phylogentic tree based on rbcL barcoding was constructed. Three clusters were shown, the first contained Moringa oleifera (R2) and the second contained Moringa oleifera (R1) and Moringa peregrina (R3). While the third cluster contained Moringa peregrina (R4). Several studies have reported similar observations. The results demonstrate that the analysis of these DNA barcode sequences is a reliable method for distinguishing between four Moringa genotypes.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"45 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85432920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Isolation and characterization of Haloferax mucsum from Red sea saltern as an halobacterial Archea capable of growing on Naphtaline as an aromatic compounds. 红海盐沼中嗜盐菌mucsum的分离与特性研究。嗜盐菌是一种能以萘为芳香族化合物生长的古菌。

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 1900-01-01 DOI: 10.5455/EGYJEBB.20181126081938

Hanan Temerk

引用次数: 0