THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)最新文献

Screening and optimization of cellulolytic activity of local microbial isolates in the Egyptian Delta region. 埃及三角洲地区微生物分离株纤维素水解活性的筛选与优化。

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230612120612

Anwer Badry, Reham Shohoud, Maha M. Azab

引用次数: 0

Isolation and Characterization of Dehydrin Gene from Egyptian Gray Mangrove in Nabq Protectorate 纳布克保护地埃及灰红树林脱氢酶基因的分离与鉴定

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20221223094631

A. Atawy, M. Rizk, M. Ahmed

{"title":"Isolation and Characterization of Dehydrin Gene from Egyptian Gray Mangrove in Nabq Protectorate","authors":"A. Atawy, M. Rizk, M. Ahmed","doi":"10.5455/egyjebb.20221223094631","DOIUrl":"https://doi.org/10.5455/egyjebb.20221223094631","url":null,"abstract":"ABSTRACT: Dehydrins have a key role in protecting plants, especially in the grey mangrove tree Avicennia marina (Forssk.) Vierh. from salt stress. Understanding the mangrove plants at the molecular level will be necessary for developing such highly salt-tolerant crops. The full-length cDNA of the DHN gene sequence AmDHN was isolated from Avicennia marina and contained a 588bp open reading frame (ORF) encoding a 195 amino acid protein with a molecular weight of about 19.746k Da. As well as, multiple alignment sequences and phylogenetic relationships were analyzed for sequences using MEGA7 software revealing that AmDHN has a high identity with other DHNs plants and suggesting that AmDHN belongs to group II (LEA) proteins. Using bioinformatics analysis tools, we have investigated characterize and gene expression to better understand the structure and function analysis prediction of the AmDHN protein. AmDHN protein contains motifs (SYK2), Sn, Yn segment, and at least one copy of a lysine-rich conserved sequence known as K-segment (consensus EKKGIME/DKIKEKLPG) near the C terminal, the 3D predicted the structure protein sequence of the secondary structure, the top 5 final models using by I-TASSER server and several parameters computed by the software were obtained. Our results indicate that the AmDHN gene plays an essential role in salt stress remediation in the Avicennia marina. They could be used for further studies to understand the molecular mechanisms of salinity tolerance in plants and can be potentially utilized in transforming other plants to improve tolerance to salinity stress.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"282 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77193152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study on microbiota isolated from olive mill wastewater disposal ponds, with emphasis on filamentous fungi and their biodegradative potential in North Sinai Governorate, Egypt 埃及北西奈省橄榄厂废水处理池微生物群的研究，重点是丝状真菌及其生物降解潜力

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230107122430

A. Shokr, M. Eweis, Wael Elrady, M. Rizk

{"title":"A study on microbiota isolated from olive mill wastewater disposal ponds, with emphasis on filamentous fungi and their biodegradative potential in North Sinai Governorate, Egypt","authors":"A. Shokr, M. Eweis, Wael Elrady, M. Rizk","doi":"10.5455/egyjebb.20230107122430","DOIUrl":"https://doi.org/10.5455/egyjebb.20230107122430","url":null,"abstract":"The microbial composition of olive mill wastewater (OMWW) from six disposal ponds at three localities in North Sinai Governorate, Egypt has been studied. Such OMWW samples contained a variable (but high) number of bacteria, yeasts, and mold. Eighty-one isolates related to 9 bacterial species were recovered, and seven common bacterial species were isolated from Bir al Abd, Al-Arish, and Rafah sites sources namely Enterococcus faecalis, Escherichia coli, Bacillus cereus, B. subtilis, Micrococcus luteus, Pseudomonas aeruginosa, and Klebsiella pneumonia. In addition to these species, Vibrio cholerae and Shigella species were isolated from the Bir al Abd site. One hundred and twenty isolates related to 13 fungal species were recovered. Seven members of different fungal genera (Aspergillus, Penicillium, Fusarium, Paecilomyces, Alternaria, Chaetomium, and Rhizopus) were recorded. Four genera were widely distributed, and they were able to grow efficiently in undiluted OMWW as a sole source of nutrients. Strains of Aspergillus, Penicillium, Fusarium, and Paecilomyces showed a marked capacity for OMWW detoxification. The physicochemical studies further revealed the presence of relatively low levels of sodium, potassium, calcium, magnesium, and iron. Nitrate concentration was relatively high, 70.13 mg/L. The biological oxygen demand (BOD) and the chemical oxygen demand (COD) recorded high values that ranged between 2210-2270 mg/L and 22760-22800 mg/L, respectively. High BOD and COD values are important indicators of high organic and inorganic components of OMWW and possible negative environmental consequences. The presence of fungal and bacterial species implied possible degradation of the effluent.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83470647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invitro antibacterial activity of some medicinal plants against food poisoning Staphylococcus aureus and Salmonella typhi 一些药用植物对食物中毒金黄色葡萄球菌和伤寒沙门氏菌的体外抑菌活性

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230119022822

H. Sheikh

引用次数: 0

Comparative Morphological Structures of Pollen Grains of Six Taxa of Silenoideae, Caryophyllaceae 标题石楠科菊科6个分类群花粉粒形态结构的比较

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230131123212

S. Teleb, Rajaa Mabrouk, Marwa M. Eldemerdash

{"title":"Comparative Morphological Structures of Pollen Grains of Six Taxa of Silenoideae, Caryophyllaceae","authors":"S. Teleb, Rajaa Mabrouk, Marwa M. Eldemerdash","doi":"10.5455/egyjebb.20230131123212","DOIUrl":"https://doi.org/10.5455/egyjebb.20230131123212","url":null,"abstract":"The morphological structures of pollens grains of six taxa belonging to 3 genera of Silenoideae, Caryophyllaceae were examined with the light and scanning electron microscope . In order to elucidate its significance in taxonomy of the group, qualitative and quantitative variables of pollen characters related to the shape, size, aperture characteristics of pores, exine characteristics include exine thickness columellae pattern (LM), tectum (LM) and Sculpture of pollen grain were studied. The pollen grains in the studied species were apolar, polypantoporate, with 12-36 pores. The dominant character of pollen ornamentation is spinulose with variably sparse spinules. Pollen size also varies among different species, and length of the grain diameter are in medium-sized (23-60μm). Cluster and principal component analysis (PCA) of qualitative and quantitative data were used to demonstrate the pollen grains similarities among the species. UPGMA tree derived from cluster analysis reveals two major clusters, the first of which consists of three species (Silene dioca, Silene vulgaris subsp. thorei and Silene pusilla. The second cluster comprises the rest of species Silene vulgaris subsp. bosniaca, Dianthus gratianopolitanus and Minuartia mutabilis, a more pollen grains description to be obtained results in comparative manner to these taxa which belong the family.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80963652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement study for production, extraction, and characterization of fungal species chitosan 壳聚糖真菌的制备、提取及特性研究

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230325111446

S. Sabbagh, Shimaa S. Hussien, H. Mira, O. Desouky, Dina M. Elgohary

{"title":"Enhancement study for production, extraction, and characterization of fungal species chitosan","authors":"S. Sabbagh, Shimaa S. Hussien, H. Mira, O. Desouky, Dina M. Elgohary","doi":"10.5455/egyjebb.20230325111446","DOIUrl":"https://doi.org/10.5455/egyjebb.20230325111446","url":null,"abstract":"Fungi and their derivatives such as chitosan able to influence the content of ores highlight the complex and dynamic nature of mineral biogeochemistry. Understanding the role of fungi in ore extraction can help to develop more sustainable and efficient mining practices in the future. Chitosan is a biopolymer is naturally originated product that can be derived from the exoskeleton of crustaceans and insects, respectively, and can also be obtained from fungal sources. Chitosan is a versatile hydrophilic polysaccharide derived from chitin. The extraction and characterization of this biopolymer from fungal isolates involves a series of chemical and physical processes to purify and modify the material. The study of the productive applications of chitosan encompasses a wide range of fields, including agriculture, medicine, and environmental management. This biopolymer has been found to have various properties such as biocompatibility, biodegradability, and antimicrobial activity, making it useful in various industries and applications. The extraction and characterization of chitosan from fungal sources and the study of their productive applications is an ongoing area of research with the potential for significant technological advancements and contributions to sustainability. Finally, applications of chitosan-based biomaterials, such as antifungal effects of were estimated by plate well diffusion method against Aspergillus ficuum, Aspergillus fumigatus and Fusarium oxysporum.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75271344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

On The Antioxidant Activity and Cytotoxicity of Echinops spinosus and Fagonia mollis in Wadi Hagul, Egypt 的抗氧化活性和细胞毒性Echinops spinosus和Fagonia mollis Wadi Hagul,埃及

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20220129014020

Magda Elaraby, H. Ahmad, Nayira Abbas

{"title":"On The Antioxidant Activity and Cytotoxicity of Echinops spinosus and Fagonia mollis in Wadi Hagul, Egypt","authors":"Magda Elaraby, H. Ahmad, Nayira Abbas","doi":"10.5455/egyjebb.20220129014020","DOIUrl":"https://doi.org/10.5455/egyjebb.20220129014020","url":null,"abstract":"Echinops spinosus and Fagonia mollis were two plants collected from Wadi Hagul, Eastern desert, Egypt during spring and autumn season. Secondary metabolites were at their highest levels in both tested plants during autumn season, and the antioxidant capabilities of these secondary metabolites were physiologically relevant to plant stress response systems. The invitro studies revealed that both plants have antioxidant properties and a promising cytotoxic activity against a breast cancer cell line (MCF-7). Based on the least of IC50 value of this extract and fractions, GC/ MS analysis was done for E. spinosus and F. mollis methylene chloride fractions and F. mollis crude extract. The GC/MS analysis disclosed that both E. spinosus and F. mollis methylene chloride fractions and their crude extracts, have predominant phytochemical compounds that have cytotoxic activity. The presence of pentanoic acid, 4-oxo-, butyl ester in E. spinosus methylene chloride fraction, and mome inositol and 9,12,15-octadecatrienoic acid, methyl ester components in F. mollis crude extract as well as phytol and (-)-loliolide in its methylene chloride fraction.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76554741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent 进口肉类中产生肠毒素和抗生素耐药基因的金黄色葡萄球菌的分子特征:作为抗菌剂的青蒿提取物

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230607015627

Shimaa Sapagh, Mohamed Monem, Esraa A. A. Badr, M. Yassine, N. Elsayed, A. Shala

{"title":"Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent","authors":"Shimaa Sapagh, Mohamed Monem, Esraa A. A. Badr, M. Yassine, N. Elsayed, A. Shala","doi":"10.5455/egyjebb.20230607015627","DOIUrl":"https://doi.org/10.5455/egyjebb.20230607015627","url":null,"abstract":"Staphylococcus aureus is a major foodborne pathogen in raw and ready-to-eat meat products. In this study, the effectiveness of methanolic extracts of Artemisia herbal-alba in inhibiting pan-resistant and enterotoxic Staphylococcus aureus was investigated. The first analysis showed a high prevalence (33%) of coagulase-positive staphylococci in imported meat samples from retail stores. Examination of antibiotic resistance patterns revealed that staphylococcal antibiogram resistance profiles were diverse: five strains demonstrated resistance to at least one antimicrobial agent, fourteen isolates demonstrated multidrug resistance (MDR) and one resistant to all test antibiotics (PAN). Multiplex PCR of pan-Staphylococcus strains revealed positive serotypes for the enterotoxin genes seb, seg and sei at 665.05 bp, 277.99 bp and 460.74 bp, respectively, and a positive serotype for the mec-A gene at 538.45 bp. Compared to the tetracycline effect, methanolic extracts of Artemisia herba-alba showed significant inhibitory effects against pan-resistant Staphylococcus aureus. Real-time quantitative PCR data analysis showed that Artemisia herba-alba methanolic extracts caused a significant decrease in the expression levels of the genes mecA, mecC, sei, seg and seb. Therefore, the methanolic extract of Artemisia herba-alba has the potential to be a promising natural product to control foodborne pathogens and could be valuable for food safety applications.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82727693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic study of (Plantaginaceae Juss.) and allied genera of Scrophulariaceae Juss∙ 车前子科(车前子科)及玄参科(车前子科)近缘属的分类研究

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230209083158

A. Shehata, W. Taia, M. Ibrahim, I. Shamy

{"title":"Taxonomic study of (Plantaginaceae Juss.) and allied genera of Scrophulariaceae Juss∙","authors":"A. Shehata, W. Taia, M. Ibrahim, I. Shamy","doi":"10.5455/egyjebb.20230209083158","DOIUrl":"https://doi.org/10.5455/egyjebb.20230209083158","url":null,"abstract":"The morphology and morphometric of stems, leaves, inflorescence, flowers and fruits of 18 species of Plantago L. (Plantaginaceae sensu stricto (s.s.) as well as 18 related taxa that were formerly assigned to the Scrophulariaceae , representing the genera : Anarrhinum, Antirrhinum, Digitalis ,Linaria, Kickxia, Russelia, Verbascum and, Veronica were investigated . To clarify the taxonomic relationships among all studied taxa and provide more information about the phenetic relationships of the investigated species of Plantago and its infrageneric classification ,107 morphological characters were numerically analyzed using the UPGMA clustering method ∙The resulting phenogram clearly showed the separation of the Plantago species from the investigated scrophulariaceous species∙ This result supports the earlier taxonomic views in the isolation of Plantago in its own mono-generic family (Plantaginaceae) apart from other Scrophulariaceous species ∙Additionally, the study showed that Veronica possessed certain affinities to Plantago species, thus giving extra support to the earlier views that Plantaginaceae sensu stricto and Scrophulariaceae sensu lato are allied through Veronica ∙Moreover , the obtained data provided some evidence for the infrageneric classification of Plantago and corresponded with the ,classification systems proposed by Rhan (1978) and Shipunov(2021)∙","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90259844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antibacterial and antioxidant potentials of some Egyptian wild plant species. 探索一些埃及野生植物的抗菌和抗氧化潜能。

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230709064439

Mohamed Aldamanhory, K. Allah, S. Shabana, Maha M. Azab

{"title":"Exploring the antibacterial and antioxidant potentials of some Egyptian wild plant species.","authors":"Mohamed Aldamanhory, K. Allah, S. Shabana, Maha M. Azab","doi":"10.5455/egyjebb.20230709064439","DOIUrl":"https://doi.org/10.5455/egyjebb.20230709064439","url":null,"abstract":"In recent years, it has been evident that there is a rise in global interest in the discovery of drugs from medicinal plants. The present study aimed to estimate the antibacterial and antioxidant activities of ethanol extract of five plant species: Lavandula atriplicifolia, Cocculus pendulus, Teucrium polium, Varthemia candicans, and Cassia javanica against multidrug-resistant bacterial isolates. In the present study, 107 different clinical specimens were collected and cultured resulting in seventy-nine bacterial isolates. The antibiotic-resistant pattern revealed the presence of seventeen multidrug-resistant bacteria. In vitro, antibacterial activity was evaluated against the seventeen resistant bacterial isolates by agar well diffusion method. Among the plants screened, the ethanol extracts of V. candicans and C. javanica showed the best antibacterial activity. The highest total phenol content was shown by L. atriplicifolia, Cassia javanica, and V. candicans ethanol extracts, while the highest flavonoid content was shown by L. atriplicifolia and T. polium extracts. The ethanol extracts of T. polium, L. atriplicifolia, V. candicans, and C. pendulus recorded significant DPPH scavenging activity, while the extracts of C. javanica, V. candicans, and T. polium recorded high PMA values. The results indicated that the investigated plants possessed significant antibacterial and antioxidant properties and could be a vital source of natural compounds for the development of novel drugs.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88060571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0