发布求助
文献互助 智能选刊 最新文献

Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences最新文献

筛选
英文 中文
大豆( Glycine max (L.)Merr.)未成熟子叶组织的体细胞胚胎诱导和植株再生的研究 大豆( Glycine max (L.)Merr.)未成熟子叶组织的体细胞胚胎诱导和植株再生的研究
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-09-10 DOI: 10.1360/ZB1988-18-9-939
冯新华, 蒋兴邨, 邵启全
{"title":"大豆( Glycine max (L.)Merr.)未成熟子叶组织的体细胞胚胎诱导和植株再生的研究","authors":"冯新华, 蒋兴邨, 邵启全","doi":"10.1360/ZB1988-18-9-939","DOIUrl":"https://doi.org/10.1360/ZB1988-18-9-939","url":null,"abstract":"本文利用大豆未成熟子叶作外植体,进行大豆体外培养并诱导体细胞胚胎形成和植株再生的研究。结果表明,高浓度生长素10mg/l NAA或5mg/l 2,4-D是大豆体细胞胚胎形成的先决条件。附加10mg/1 NAA的Murashige和Skoog培养基(简称MS培养基)能够直接在子叶表面诱导体细胞胚的形成,其频率可达85％;附加5mg/l 2,4-D的MS培养基首先诱导未成熟子叶产生胚性愈伤组织,然后产生大量体细胞胚,频率高达94％。我们成功地获得再生的大豆完整小植株15株,消除了大豆基因工程的障碍之一。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121652585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EXPRESSION OF A ZEIN GENE (Z4) INTRODUCED INTO DICOTYLEDONOUS Solanum nigrum 玉米蛋白基因Z4在双子叶植物茄中的表达
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-09-10 DOI: 10.1360/YB1988-31-9-1085
Dene Wanyin, Shao Qiquan, Jiang Xingcun
{"title":"EXPRESSION OF A ZEIN GENE (Z4) INTRODUCED INTO DICOTYLEDONOUS Solanum nigrum","authors":"Dene Wanyin, Shao Qiquan, Jiang Xingcun","doi":"10.1360/YB1988-31-9-1085","DOIUrl":"https://doi.org/10.1360/YB1988-31-9-1085","url":null,"abstract":"A maize genomic clone containing a zein gene (Z4) is inserted into the T-DNA of the Ti plasmid pTiT37. Agrobacterium tumefaciens strain harboring this modified Ti plasmid is used to infect stem sections of young plants or explants of dicotyledonous Solanum nigrum. Axenic transformed calli active in nopaline synthesis are obtained and transgenic plants are differentiated from them DNA Southern hybridization and RNA dot-hybridization analyses show that the zein gene is really transferred and integrated into the nuclear genome of transformed Solanum nigrum and that the zein gene can be transcribed into mRNA in the transformed calli and shoots. But the presence of the zein protein cannot be detected in either the transformed calli or the transgenic shoots. The results of thte experiments demonstrate that the promoter of a gene from monocotyledonous plants can function normally in transgenic dicots. The possibility of developmentally-regulated expression of the zein gene in transformed dicots is discussed in the article.","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115864976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
三核钼簇合物的配基置换反应及{[Mo 3 ( μ 3 —S)( μ —S) 3 (dtp) 3 (C 3 H 4 N 2 ) 3 ] + (dtp) - }·(CH 3 ) 2 CO(I)的合成和晶体结构 三核钼簇合物的配基置换反应及{[Mo 3 ( μ 3 —S)( μ —S) 3 (dtp) 3 (C 3 H 4 N 2 ) 3 ] + (dtp) - }·(CH 3 ) 2 CO(I)的合成和晶体结构
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-08-10 DOI: 10.1360/ZB1988-18-8-796
卢绍芳, 黄明东, 黄建全, 黄金陵, 卢嘉锡
{"title":"三核钼簇合物的配基置换反应及{[Mo 3 ( μ 3 —S)( μ —S) 3 (dtp) 3 (C 3 H 4 N 2 ) 3 ] + (dtp) - }·(CH 3 ) 2 CO(I)的合成和晶体结构","authors":"卢绍芳, 黄明东, 黄建全, 黄金陵, 卢嘉锡","doi":"10.1360/ZB1988-18-8-796","DOIUrl":"https://doi.org/10.1360/ZB1988-18-8-796","url":null,"abstract":"具有松散配位的三核钼簇合物[Mo 3 ( μ 3 —S)( μ —S) 3 (dtp) 3 ·(H 2 O)](A)(dtp=S 2 P(OC 2 H 5 ) 2 )和过量的咪唑反应时,(H 2 O)配体和双啮桥基配体(dtp)可以同时被置换而形成标题化合物(Ⅰ)。晶体结构属三斜晶系,空间群P1。结构组成包括簇阳离子Mo 3 ( μ 3 —S)( μ —S) 3 (dtp) 3 ·(C 3 H 4 N 2 ) 3 ],(dtp)阴离子及丙酮溶剂。簇阳离子具有近似C 3 对称性,三个Mo原子基本上形成等边三角形,其Mo—Mo键长分别为2.763(1),2.762(1),和2.756(1),此簇合物是配体取代反应系列研究的产物之一,是由已知原子簇反应获得新型簇合物的一个例证。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116803669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
药物离子选择性电极响应规律的系统研究——III.红霉素电极及其对季铵的选择性规律 药物离子选择性电极响应规律的系统研究——III.红霉素电极及其对季铵的选择性规律
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-08-10 DOI: 10.1360/ZB1988-18-8-862
姚守拙, 肖俊, 聂利华
{"title":"药物离子选择性电极响应规律的系统研究——III.红霉素电极及其对季铵的选择性规律","authors":"姚守拙, 肖俊, 聂利华","doi":"10.1360/ZB1988-18-8-862","DOIUrl":"https://doi.org/10.1360/ZB1988-18-8-862","url":null,"abstract":"本文研究了多种红霉素电极。电极对于季铵的选择性可用诱导效应和空间位阻效应来定量说明,符合方程式log K ij = a 1 I- a 2 Z/R+ a 0 ,式中I为诱导效应指数, a 1 , a 2 , a 0 为与电活性物有关的系数。建立了阳离子型药物电极 K ij 对季铵烷基链碳原子数 n 的关系的一般数学模型:log K ij =A/2.7 n +B/(0.80+1.26n)+C。证明:随着碳原子数增大,log K ij 趋于一极限值C。此处A,B,C为与电活性物有关的系数。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123694877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
根据沉积矿物的共生组合恢复大气CO 2 气分压值的演化 根据沉积矿物的共生组合恢复大气CO 2 气分压值的演化
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-07-10 DOI: 10.1360/ZB1988-18-7-747
陈福 朱笑青
{"title":"根据沉积矿物的共生组合恢复大气CO 2 气分压值的演化","authors":"陈福 朱笑青","doi":"10.1360/ZB1988-18-7-747","DOIUrl":"https://doi.org/10.1360/ZB1988-18-7-747","url":null,"abstract":"本文依据海水pH值演化和海气平衡理论,讨论了根据地层中沉积矿物共生组合恢复大气CO 2 气分压值的理论和方法,研究了海水pH值的演化与碳酸岩层沉淀的关系,计算了地层中一系列与碳酸盐矿物伴生的沉积矿物组合生成的pH值范围及其所对应的大气CO 2 气分压值。指出太古代沉积碳酸岩层虽然很少,但大气CO 2 气含量却很高,且逐渐增多,直至形成稠密CO 2 气圈。26×10 2 Ma前后,CO 2 气分压曾达10—50atm。从早元古代至现代表现为碳酸岩层沉积层位逐渐升高。最后超越硫化物层和硅酸盐粘土矿物层的总趋势,与此相应大气CO 2 气分压值逐渐降低,由稠密CO 2 气圈降为现在的0.03％,按CO 2 气分压值的范围和波动特点可将其历史演变划分为三个历史阶段。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121110138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
玉米醇溶蛋白基因Z 4 在双子叶植物龙葵( Solanum nigrum )中的表达 玉米醇溶蛋白基因Z 4 在双子叶植物龙葵( Solanum nigrum )中的表达
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-07-10 DOI: 10.1360/ZB1988-18-7-719
邓万银, 邵启全, 蒋兴邨
{"title":"玉米醇溶蛋白基因Z 4 在双子叶植物龙葵( Solanum nigrum )中的表达","authors":"邓万银, 邵启全, 蒋兴邨","doi":"10.1360/ZB1988-18-7-719","DOIUrl":"https://doi.org/10.1360/ZB1988-18-7-719","url":null,"abstract":"本文将含有玉米醇溶蛋白基因Z 4 的玉米基因组克隆插入Ti质粒pTiT 37 的T-DNA区。用带有此质粒的根癌农杆菌感染龙葵,得到了能合成胭脂碱的转化愈伤组织和转化植株。DNA分子杂交和RNA点杂交表明,Z 4 基因确实转化并整合进了龙葵的核基因组,并且被转录成mRNA。但在转化体中,没能检测出玉米醇溶蛋白。实验证明,单子叶植物基因的启动子能够在双子叶植物中发挥功能。文中讨论了Z 4 基因在双子叶转化植物的种子中受发育调节的可能性。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129903762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
单元轨道线性组合近似理论——II.非周期性边界条件下聚合物的量子化学计算方法 单元轨道线性组合近似理论——II.非周期性边界条件下聚合物的量子化学计算方法
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-07-10 DOI: 10.1360/ZB1988-18-7-691
叶元杰, 于恒泰, 唐敖庆
{"title":"单元轨道线性组合近似理论——II.非周期性边界条件下聚合物的量子化学计算方法","authors":"叶元杰, 于恒泰, 唐敖庆","doi":"10.1360/ZB1988-18-7-691","DOIUrl":"https://doi.org/10.1360/ZB1988-18-7-691","url":null,"abstract":"本文提出了一种新的量子化学计算方法,得到了紧近邻近似下的本征方程,推导了用于计算带端基官能团的等规聚合物体系的计算公式。以聚乙炔体系作为算例,所得结果表明,本文提出的方法作为非周期边界条件下等规聚合物的量子化学计算方法是有效的。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127144899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TRANSFORMATION OF CULTURED HUMAN FETAL GASTRIC FIBROBLASTS WITH PHYSICO-CHEMICAL AGENTS 物化试剂对培养人胎儿胃成纤维细胞的转化作用
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-07-10 DOI: 10.1360/YB1988-31-7-850
Su Zhaozhong, Luo Zuyu, Guo Lanping, Lu Shouzhen, Liu Yalun
{"title":"TRANSFORMATION OF CULTURED HUMAN FETAL GASTRIC FIBROBLASTS WITH PHYSICO-CHEMICAL AGENTS","authors":"Su Zhaozhong, Luo Zuyu, Guo Lanping, Lu Shouzhen, Liu Yalun","doi":"10.1360/YB1988-31-7-850","DOIUrl":"https://doi.org/10.1360/YB1988-31-7-850","url":null,"abstract":"Treatment of early passage human fetal gastric fibrohlasts with ultraviolet (UV) light and the chemical carcinogen ethyl nitrosourea (ENU) in succession resulted in an immortally growing cell line, named GTS 8502. The cells of this line display typical transformation characteristics, such as irregularly shaped nuclei, heteroploidization of karyotype and frequent appearance of heteromorphic chromosomes, the enhanced volume ratio of nucleus to cytoplasm, multinucleoli, appearance of microvilli on the surface of the cells and agglutination reaction to lectin concanavalin A. The transformants have high growing and mitotic indices and the ability of focus-formation on monolayers and anchorage independent growth in soft agar medium. Moreover, these cells induced turnouts in nude mice or in immunosuppressed new-born rats through heterotransplantation. The results of various methods including electromicroscopy and histochemical analyses indicate that GTS 8502 cells are of fibroblast origin.Our results thus indicate that synergism of two carcinogens may raise the transformation ratio of normal human cells, which is otherwise extremely low. The transformation of human cells may be utilized to detect environmental carcinogens.","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124589706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE DISTRIBUTION OF P ELEMENTS IN D. melanogaster ALONG THE COAST OF CHINA STUDIED BY DNA HYBRIDIZATION METHOD 摘要用DNA杂交方法研究了中国沿海黑腹龙葵中磷元素的分布
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-05-10 DOI: 10.1360/YB1988-31-5-563
L. Fang, Zhao Shou-yuan, Jiang Zhiling, L. Zu-dong, Sun Tinwu
{"title":"THE DISTRIBUTION OF P ELEMENTS IN D. melanogaster ALONG THE COAST OF CHINA STUDIED BY DNA HYBRIDIZATION METHOD","authors":"L. Fang, Zhao Shou-yuan, Jiang Zhiling, L. Zu-dong, Sun Tinwu","doi":"10.1360/YB1988-31-5-563","DOIUrl":"https://doi.org/10.1360/YB1988-31-5-563","url":null,"abstract":"The distribution of the P-M system in four local Drosophila melanogaster populations obtained insouthern China has been examined by mating tests. There was no P strain. A HindⅢ fragnment ofthe P element has been cloned, named as pPH 0.86, and used as probe in this study. The distribu-tion of the P element itself in thirteen local D. melanogaster populations along the eastern coast ofChina has been studied by the method of DNA hybridization. It is found that all the populations ob-tained from the north of Shanghai carry P elements but few populations from the south of Shanghai carry Pelements. In comparison with the mating test, the method of DNA hybridization is more direct andreliable.The origin and trends of distribution of P elements in China are discussed in this paper.","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114925350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
小鼠肝果糖6-磷酸,2-激酶的纯化及动力学研究 小鼠肝果糖6-磷酸,2-激酶的纯化及动力学研究
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1988-05-10 DOI: 10.1360/ZB1988-18-5-499
李林 许根俊
{"title":"小鼠肝果糖6-磷酸,2-激酶的纯化及动力学研究","authors":"李林 许根俊","doi":"10.1360/ZB1988-18-5-499","DOIUrl":"https://doi.org/10.1360/ZB1988-18-5-499","url":null,"abstract":"经过超离心,聚乙二醇分级沉淀及DEAE-Sephadex,Blue-Sepbarose。磷酸纤维素三种柱层析等一系列过程,纯化了小白鼠肝脏的果糖6-磷酸,2-激酶。经凝胶过滤和SDS聚丙烯酰胺电泳方法测出该酶是由两个相同亚基构成的分子量为110000的蛋白。Mg 2+ 是其活性所必需的,活化方式呈正协同性。酶与底物的结合方式,对果糖6-磷酸表现正协同性,对ATP无协同性,其Km值随果糖6-磷酸的浓度减小而增大,表明酶的作用为顺序机制。活性中心有一个必需氨基酸残基与结合ATP有关,结合后,其侧链pKa由9.5移至9.8。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1988-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126427548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书