Teratogenesis, carcinogenesis, and mutagenesis最新文献_第7页

Re: Diabetes teratogenicity in mice is accompanied with distorted expression of TGF‐β2 in the uterus. Fein et al. 2001;22:59–71. Re:小鼠的糖尿病致畸性伴随着子宫中TGF - β2的扭曲表达。费恩等人。2001;22:59-71。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10022

H. Kalter

引用次数: 0

Inhibition of telomerase activity in endometrial cancer cells by selenium-cisplatin conjugate despite suppression of its DNA-damaging activity by sodium ascorbate. 硒-顺铂偶联物对子宫内膜癌细胞端粒酶活性的抑制作用，尽管抗坏血酸钠抑制其dna损伤活性。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.1040

J. Błasiak, Magdalena Kadłubek, J. Kowalik, H. Romanowicz‐Makowska, T. Pertyński

{"title":"Inhibition of telomerase activity in endometrial cancer cells by selenium-cisplatin conjugate despite suppression of its DNA-damaging activity by sodium ascorbate.","authors":"J. Błasiak, Magdalena Kadłubek, J. Kowalik, H. Romanowicz‐Makowska, T. Pertyński","doi":"10.1002/TCM.1040","DOIUrl":"https://doi.org/10.1002/TCM.1040","url":null,"abstract":"Telomerase activation can be considered as a critical step in cell immortalization. The enzyme elongates or maintains telomere length by adding to its end tandem TTAGGG repeats by using its endogenous RNA template. Telomerase is not detectable in most somatic cells but is upregulated in germ line cells and in 85-90% of human cancers, which suggests important role of telomerase in neoplastic transformation. Consequently, telomerase has been proposed as a potentially highly selective target for the development of antiproliferative agents. Platinum complexes are widely administrated in cancer therapy. A conjugate of selenite with diammineplatinum [(NH(3))(2)Pt(SeO(3))(2)] is a novel potential anticancer drug. Using alkaline single cell gel electrophoresis (comet assay), we showed that the drug at 5-30 microM induced concentration-dependent damage to DNA of endometrial cancer cells derived from tumor samples. Sodium ascorbate at 10 and 50 microM reduced the extent of the DNA damage evoked by the drug. (NH(3))(2)Pt(SeO(3)) reduced telomerase activity in the cells in a concentration-dependent manner as measured by using the telomere repeat amplification protocol (TRAP) assay. This effect was independent of sodium ascorbate. Therefore, mutagenic effects of the conjugate can be reduced by well-recognized antimutagen, sodium ascorbate, but it can still retain ability to affect neoplastic transformation. The results obtained indicate that (NH(3))(2)Pt(SeO(3)) may specifically inhibit telomerase activity in endometrial cancer cells.","PeriodicalId":22336,"journal":{"name":"Teratogenesis, carcinogenesis, and mutagenesis","volume":"13 1","pages":"73-82"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74193310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Re: Diabetes teratogenicity in mice is accompanied with distorted expression of TGF-β2 in the uterus. Fein et al. 2001;22:59–71. Response to H. Kalter. Re:小鼠的糖尿病致畸性伴随着子宫内TGF-β2的畸变表达。费恩等人。2001;22:59-71。对H. Kalter的回应。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10023

A. Fein, N. Magid, H. Carp, V. Toder, A. Torchinsky

引用次数: 0

Preliminary evaluation of the cytotoxic and genotoxic potential of D-003: Mixture of very long chain fatty acids. D-003:超长链脂肪酸混合物的细胞毒性和基因毒性潜力的初步评价。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10001

R. Gámez, I. Rodeiro, I. Fernández, P. Acosta

{"title":"Preliminary evaluation of the cytotoxic and genotoxic potential of D-003: Mixture of very long chain fatty acids.","authors":"R. Gámez, I. Rodeiro, I. Fernández, P. Acosta","doi":"10.1002/TCM.10001","DOIUrl":"https://doi.org/10.1002/TCM.10001","url":null,"abstract":"D-003 is a mixture of very long chain aliphatic acids purified from sugar cane wax, wherein octacosanoic acid represents the major component. Previous experimental studies have shown that D-003 inhibits platelet aggregation in rodents. Also, its lowers total (TC) and low-density lipoprotein cholesterol (LDL-C) in normocholesterolemic rabbits in a dose-dependent manner and inhibits cholesterol biosynthesis in fibroblast cultures. The present study was performed to investigate the in vitro cytotoxic and genotoxic potential effects of D-003 assessed through two tests: the neutral red (NR) assay and the Ames test. Positive and negative controls were included in each experimental series. Compared with controls, no cytotoxicity was evident after 24 and 72 h of treatment with doses up to 1,000 microg/ml in the NR assay. On the other hand, D-003 (5-5,000 microg/plate) did not increase the frequency of reverse mutations in the Ames test in both alternatives with or without S9 mix metabolic activation and a pre-incubation step. The positive control chemicals included in each experiment, namely, treatment with sodium dodecyl sulphate (SDS) in the NR assay and sodium azide (NaAz), 2-aminofluorene (AF), and dimethylnitrosamine (DMNA) in the Ames test, induced the expected changes, such as a decrease in optical density (OD) values in the NR assay and an increase in the frequency of reverse mutations in the Ames test. The present results indicate that D-003 did not show evidence of cytotoxic or genotoxic potential in tests able to detect the ability of chemicals to disrupt cells (NR assay) or to induce gene mutations (Ames test).","PeriodicalId":22336,"journal":{"name":"Teratogenesis, carcinogenesis, and mutagenesis","volume":"19 1","pages":"175-81"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86317391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Effects of fasting and intermittent fasting on rat hepatocarcinogenesis induced by diethylnitrosamine. 禁食和间歇禁食对二乙基亚硝胺致大鼠肝癌的影响。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10005

N. Rocha, L. F. Barbisan, M. L. de Oliveira, J. D. de Camargo

{"title":"Effects of fasting and intermittent fasting on rat hepatocarcinogenesis induced by diethylnitrosamine.","authors":"N. Rocha, L. F. Barbisan, M. L. de Oliveira, J. D. de Camargo","doi":"10.1002/TCM.10005","DOIUrl":"https://doi.org/10.1002/TCM.10005","url":null,"abstract":"The influences of fasting on DEN-initiation and of intermittent fasting (IF) on the rat liver chemical carcinogenesis process were evaluated in a 52-week long assay. Three groups of adult male Wistar rats were used: Groups 1 to 3 were treated with a single i.p. injection of 200 mg/kg of diethylnitrosamine (DEN). Group 2 was submitted to 48 h fasting prior to DEN treatment. After the 4th week, Group 3 was submitted to IF, established as 48 h weekly fasting during 48 weeks, while Groups 1 and 2 were fed ad libitum until the 52nd week. All animals were submitted to 70% partial hepatectomy and sacrificed at the 3rd and 52nd weeks, respectively. Fasting prior to DEN-initiation did not influence the development of altered foci of hepatocytes (AFHs) and of hepatic nodules (Group 2 vs. Group G1). IF inhibited the development of preneoplastic lesions, since this dietary regimen decreased the number and the size of glutathione S-transferase (GST-P) positive foci and the number and size of liver nodules (Group G3 vs. Group G1). The inhibitory effect of IF was also reflected in the development of clear and basophilic cell foci. These results indicate that long-term IF regimen exerts an anti-promoting effect on rat hepatocarcinogenesis induced by DEN.","PeriodicalId":22336,"journal":{"name":"Teratogenesis, carcinogenesis, and mutagenesis","volume":"1 1","pages":"129-38"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90807852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Analysis of the cytotoxicity and mutagenicity of drinking water disinfection by-products in Salmonella typhimurium. 鼠伤寒沙门菌饮用水消毒副产物的细胞毒性及致突变性分析。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10010

Yahya Kargalioglu, B. McMillan, R. Minear, M. Plewa

{"title":"Analysis of the cytotoxicity and mutagenicity of drinking water disinfection by-products in Salmonella typhimurium.","authors":"Yahya Kargalioglu, B. McMillan, R. Minear, M. Plewa","doi":"10.1002/TCM.10010","DOIUrl":"https://doi.org/10.1002/TCM.10010","url":null,"abstract":"We analyzed the cytotoxicity and mutagenicity of the drinking water disinfection by-products (DBPs) bromoform (BF), bromoacetic acid (BA), dibromoacetic acid (DBA), tribromoacetic acid (TCA), chloroform (CF), chloroacetic acid (CA), dichloroacetic acid (DCA), trichloroacetic acid (TCA), 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone (MX), and potassium bromate (KBrO3) in Salmonella typhimurium strains TA98, TA100, and RSJ100 +/- S9. Solvent controls of DMSO and ethanol and a positive control of ethylmethanesulfonate (EMS) were also analyzed. We developed a rapid microplate-based method to determine the cytotoxicity of the DBPs and we determined their mutagenic potencies. The distributions of the rank order for the cytotoxicity and mutagenicity of these DBPs were compared and the structure-function relationships were identified. TA100 -S9 was the most sensitive strain for these DBPs. The rank order of the mutagenic potency adjusted with a cytoxicity factor was MX > BA > EMS > DBA > DCA > CA with TBA, TCA, BF, and CF not mutagenic. From a structure-function perspective, the brominated acetic acids were more cytotoxic and mutagenic than their chlorinated analogs. BA was 150x more mutagenic than CA. The mutagenic potency of the haloacetic acids was inversely related to the number of halogen atoms of the molecule. BA was 36x more mutagenic than DBA. The differential cytotoxicity expressed by the DBPs indicated that a cytotoxicity analysis enhanced the sensitivity of the mutagenicity data, which resulted in an enhanced precision for comparing their relative mutagenic strengths. This information is critical when conducting quantitative structure-function analysis of these hazardous agents.","PeriodicalId":22336,"journal":{"name":"Teratogenesis, carcinogenesis, and mutagenesis","volume":"38 1","pages":"113-28"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91301901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 110

Mushroom-derived preparations in the prevention of H2O2-induced oxidative damage to cellular DNA. 蘑菇衍生制剂在预防h2o2诱导的细胞DNA氧化损伤中的作用。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10008

Yu-ling Shi, A. James, I. Benzie, J. Buswell

引用次数: 68

Alcohol induction of liver nuclear ethanol and N-nitrosodimethylamine metabolism to reactive metabolites. 酒精诱导肝核乙醇和n -亚硝基二甲胺代谢为反应性代谢物。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10009

M. I. Gómez, E. Valles, S. Fanelli, A. M. de Layño, G. D. Castro, J. Castro

{"title":"Alcohol induction of liver nuclear ethanol and N-nitrosodimethylamine metabolism to reactive metabolites.","authors":"M. I. Gómez, E. Valles, S. Fanelli, A. M. de Layño, G. D. Castro, J. Castro","doi":"10.1002/TCM.10009","DOIUrl":"https://doi.org/10.1002/TCM.10009","url":null,"abstract":"In previous studies from our laboratory we reported the presence in highly purified liver nuclei, free of contamination with other organelles, of an ethanol metabolizing system (NEMS) able to lead to acetaldehyde and 1-hydroxyethyl free radicals (1HEt). In the present study we tested whether this NEMS is inducible by chronic alcohol administration to rats and whether these nuclei also have increased ability to bioactivate N-nitrosodimethylamine (NDMA). Sprague Dawley male rats (125-150g) were fed with a nutritionally adequate liquid diet containing alcohol to provide 36% of total energy (standard Lieber-De Carli rat diet), for 28 days. Controls received an isocaloric diet without alcohol. Animals were sacrificed, livers were excised and microsomes and purified nuclear fractions were prepared. Both microsomes and nuclei from treated animals had significantly increased ability compared to controls, to biotransform ethanol to acetaldehyde using NADPH as cofactor under an air atmosphere. Both organelles also exhibited significantly increased capacity compared to controls, to bioactivate NDMA to formaldehyde and to reactive metabolites that bind covalently to proteins. Nuclear preparations from control animals were also able to metabolize NDMA to formaldehyde and reactive metabolites. Results indicate that liver nuclei may have a CYP2E1 able to bioactivate both NDMA and EtOH and that these processes are being induced by chronic alcohol drinking. The bioactivation of these xenobiotics to reactive metabolites in the neighborhood of nuclear proteins and DNA might have significant toxicological implications.","PeriodicalId":22336,"journal":{"name":"Teratogenesis, carcinogenesis, and mutagenesis","volume":"58 1","pages":"139-45"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80716584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Lipid peroxidation, DNA damage, and cellular morphology of R1 Rhabdomyosarcoma cell line irradiated in vitro by gamma-rays with different dose rates. 不同剂量率γ射线体外照射R1横纹肌肉瘤细胞系的脂质过氧化、DNA损伤和细胞形态

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10006

W. Przybyszewski, M. Wideł, O. Palyvoda

{"title":"Lipid peroxidation, DNA damage, and cellular morphology of R1 Rhabdomyosarcoma cell line irradiated in vitro by gamma-rays with different dose rates.","authors":"W. Przybyszewski, M. Wideł, O. Palyvoda","doi":"10.1002/TCM.10006","DOIUrl":"https://doi.org/10.1002/TCM.10006","url":null,"abstract":"The study examines the relationship between lipid peroxidation, DNA damage, and cell morphology after the exposure of R1 Rhabdomyosarcoma cells to two different dose-rates of gamma rays. Exponential cultures of R1 cells were irradiated with single dose of 5 Gy at high dose rate (0.833 Gy/min) and low dose rate (0.0707 Gy/min). The concentration of two aldehydes, malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), were determined. DNA damage induction and repair were measured by using the alkaline version of the comet assay. Cellular alteration was also estimated microscopically as was the frequency of cells with micronuclei and proportion of apoptosis and necrosis. These parameters were evaluated immediately (time 0) and after different times up to 48 h of incubation in 37 degrees C, after irradiation. Results indicate that a low dose rate in comparison to high dose rate caused a significantly higher increase of aldehydes concentration observed at 12 h, followed by obviously higher DNA damage at 48 h and altered cellular morphology. The inverse dose-rate effect estimated for the gamma rays Co-60 source was found to be related to the measured biochemical and morphological parameters.","PeriodicalId":22336,"journal":{"name":"Teratogenesis, carcinogenesis, and mutagenesis","volume":"121 1","pages":"93-102"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72702694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm. 在体外补充异黄酮可减少过氧化氢引起的精子DNA损伤。

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI: 10.1002/TCM.10015

J. Sierens, J. Hartley, M. J. Campbell, A. Leathem, J. Woodside

{"title":"In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm.","authors":"J. Sierens, J. Hartley, M. J. Campbell, A. Leathem, J. Woodside","doi":"10.1002/TCM.10015","DOIUrl":"https://doi.org/10.1002/TCM.10015","url":null,"abstract":"Isoflavones are plant compounds, proposed to have health benefits in a variety of human diseases, including coronary heart disease and endocrine-responsive cancers. Their physiological effects include possible antioxidant activity, therefore suggesting a role for isoflavones in the prevention of male infertility. The aim of this study was to test the antioxidant effects of the isoflavones genistein and equol on sperm DNA integrity, assessed in vitro after hydrogen peroxide-mediated damage, using the comet assay. Pre-treatment with genistein or equol at doses of 0.01-100 micromol/l significantly protected sperm DNA against oxidative damage. Both ascorbic acid (10-600 micromol/l) and alpha-tocopherol (1-100 micromol/l) also protected. Compared with ascorbic acid and alpha-tocopherol, added at physiological concentrations, genistein was the most potent antioxidant, followed by equol, ascorbic acid, and alpha-tocopherol. Genistein and equol added in combination were more protective than when added singly. Based on these preliminary data, which are similar to those observed previously in lymphocytes, these compounds may have a role to play in antioxidant protection against male infertility.","PeriodicalId":22336,"journal":{"name":"Teratogenesis, carcinogenesis, and mutagenesis","volume":"23 1","pages":"227-34"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77381144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 114