In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm.

Teratogenesis, carcinogenesis, and mutagenesis Pub Date : 2002-01-01 DOI:10.1002/TCM.10015

J. Sierens, J. Hartley, M. J. Campbell, A. Leathem, J. Woodside

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引用次数: 114

Abstract

Isoflavones are plant compounds, proposed to have health benefits in a variety of human diseases, including coronary heart disease and endocrine-responsive cancers. Their physiological effects include possible antioxidant activity, therefore suggesting a role for isoflavones in the prevention of male infertility. The aim of this study was to test the antioxidant effects of the isoflavones genistein and equol on sperm DNA integrity, assessed in vitro after hydrogen peroxide-mediated damage, using the comet assay. Pre-treatment with genistein or equol at doses of 0.01-100 micromol/l significantly protected sperm DNA against oxidative damage. Both ascorbic acid (10-600 micromol/l) and alpha-tocopherol (1-100 micromol/l) also protected. Compared with ascorbic acid and alpha-tocopherol, added at physiological concentrations, genistein was the most potent antioxidant, followed by equol, ascorbic acid, and alpha-tocopherol. Genistein and equol added in combination were more protective than when added singly. Based on these preliminary data, which are similar to those observed previously in lymphocytes, these compounds may have a role to play in antioxidant protection against male infertility.

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在体外补充异黄酮可减少过氧化氢引起的精子DNA损伤。

异黄酮是一种植物化合物，被认为对多种人类疾病有健康益处，包括冠心病和内分泌反应性癌症。它们的生理作用包括可能的抗氧化活性，因此表明异黄酮在预防男性不育方面的作用。本研究的目的是测试异黄酮染料木素和雌马酚对精子DNA完整性的抗氧化作用，在体外过氧化氢介导的损伤后，使用彗星试验进行评估。0.01 ~ 100微摩尔/升染料木素或雌马酚预处理可显著保护精子DNA免受氧化损伤。抗坏血酸(10-600微mol/l)和α -生育酚(1-100微mol/l)也有保护作用。与生理浓度的抗坏血酸和α -生育酚相比，染料木素是最有效的抗氧化剂，其次是马酚、抗坏血酸和α -生育酚。染料木素和雌马酚联合添加比单独添加更有保护作用。根据这些初步数据，这些数据与之前在淋巴细胞中观察到的相似，这些化合物可能在抗氧化保护男性不育方面发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Teratogenesis, carcinogenesis, and mutagenesis

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