Systematic Reviews in Pharmacy最新文献

{"title":"Alterations in Kidney of Albino Rat due to Acrylamide Exposure and the Possible Protective Role of l-arginine (Biochemical, Histological, Immunohistochemical and Molecular Study)","authors":"Ghada Ali El Fakahany, S. Nassar, S. Elballat","doi":"10.31838/SRP.2021.3.103","DOIUrl":"https://doi.org/10.31838/SRP.2021.3.103","url":null,"abstract":"Acrylamide (ACR): is an industrially conjugated reactive molecule that initiates cellular toxicity, induced oxidative stress and classified as a probable human carcinogen. L-arginine (L-Arg) produces nitric oxide (NO): that involved in vascular regulation, immune activity, and able to eliminate intracellular pathogens. This work conducted to evaluate nephrotoxicity of ACR and the possible protective role of L-Arg at biochemical, histopathological, immunohistochemical and molecular levels. Forty male rats were divided equally into four groups; control: received the ordinary water and diet. ACR: Animals were given a dose of ACR (50mg/kg/day): dissolved in water. ACR + L- Arg: Animals given the same dose of ACR together with L-Arg dissolved in water. L-Arg: Animals administered L-Arg by a dose of 200 mg/kg/day dissolved in water. The exposure period was a month. Kidneys were removed and prepared for histopathological, immunohistochemical studies (caspase3). Also, kidney specimens taken for real-time-PCR technique to measure p53 expression and comet assay. Blood samples were collected for kidney function detection. Animals exposed to ACR showed several histopathological lesions including glomerular shrinkage, loss of PCT brush borders, degeneration of renal epithelia, deposition of hyaline casts and necrotic areas in the renal parenchyma. significant increase in p53 expression, significant increase in caspase 3 activity in renal cells and increase in creatinine levels. However, urea levels recorded a significant decrease. Animals treated with L-Arg together with ACR showed a marked improvement in all these parameters towards the normal status. The study suggested that the administration of L-Arg provided a protective potential against ACR-nephrotoxicity.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74709076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Receiver Operating Characteristic Analysis Of HIF-1A Mrna Gene Expression Fold As Prognostic Indicator In Different Clinic-Pathological Status Of Breast Cancer -","authors":"H. H. Kzar, Haider O. Mossa Al-Gazali, M. Al-Gazally, M. Wtwt","doi":"10.31838/SRP.2021.2.37","DOIUrl":"https://doi.org/10.31838/SRP.2021.2.37","url":null,"abstract":"Background and objective: The highly prevalent and mortality type of cancer among women worldwide is breast cancer (BC). The mortality cases of BC are probably incidence by inadequate of the benefits of treatment and early detection, moreover the lack of appropriate facilities for diagnosis and detection. The aim of this work is to assess of using of ROC of HIF-1A mRNA GEF as a prognostic indicator in BC. Methods: The Total RNA was extracted from whole blood of both women with BC (n=120) and control (n=120) groups, then converted to cDNA. Amplification of HIF-1A gene (gene of interest, GOI-1) and GAPDH (as housekeeping gene, HKG) were done by quantitative real time polymerase chain reaction (qRT-PCR). ROC analysis was done by using QI Macros for Excel software. Graphs were prepared using Microsoft excel 2010. Results: The results of present study suggested that significant differences between the GER in women with BC (4.58) and GER in control group ( 1.03) (p-value< 0.05). The ROC curve analysis of GEF of HIF-1A mRNA as prognostic marker in women with BC group showed an area under the curve (AUC) of (0.833)(95% CI, 0.76-0.90) with a sensitivity of (82%) and specificity of (63%) at a cut off value 20.5. Conclusion: HIF-1A mRNA GEF is good prognostic indicator for diagnosis and differentiation between both metastasis and non- metastasis BC groups.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73204608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics Of Metformin's Cardioprotective Effect In Acute Doxorubicin Induced- Cardiotoxicity In Rats","authors":"L. Karim, I. Arif, Fouad A. Al Saady","doi":"10.31838/SRP.2021.3.18","DOIUrl":"https://doi.org/10.31838/SRP.2021.3.18","url":null,"abstract":"Doxorubicin (DOX) is a powerful anticancer agent with sever cardiotoxic side effect which limits the clinical use. Metformin (MET) is antihyperglycemic drug with potential cardioprotective effect via AMP-activated protein kinase (AMPK) (increases fatty acid oxidation, decreases the production of ROS, maintaining energy homeostasis and apoptosis). Metabolomics technology deals with systematic study of chemical fingerprints of metabolite profiles. Different metabolic processes can be identified which will give information of any change in the metabolic profile of tissues as well as of biofluids after drug administration. This research designed to investigate MET cardioprotective effect against acute cardiotoxicity induced by DOX using metabolomics technology. Methods: Twenty four adult male wistar rats divided into four groups (6 animals each): control group (saline, i.p.); MET group (300mg/kg/day for 7dayes) by gavage; DOX group (20 mg/kg,i.p.) for acute induction of cardiotoxicity; Met + DOX group received DOX (20mg/kg i.p.) and Met (300 mg/kg/day, for 7 days, starting five days prior to DOX treatment) orally with gavage. Assessment of heart tissue metabolomics, serum MDA and GSH in addition to trichrome stain. Results: The results showed that pretreatment with MET (MET+DOX) significantly (p","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72748611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risks Advantages and Early Workability Diagnosis of CT Lung Cancer of Smokers Contaminated with HIV","authors":"A. Iqbal, M. Younas, M. A. Tayyab, M. Yoosuf, Shumaila Sohail, Uzma Batool","doi":"10.31838/SRP.2021.4.23","DOIUrl":"https://doi.org/10.31838/SRP.2021.4.23","url":null,"abstract":"Aim: Cellular breakdown in the lungs screening with Chest registered Tomography (CT) is gainful in smokers matured 55 to 74 years. Methods: We considered the dangers, advantages and achievability of ahead of schedule cellular breakdown in the lung’s analysis with CT in HIV-contaminated smokers. French, multicenter, single round chest CT concentrate in France, acknowledged between May 2019 and April 2020. Our current research was conducted at Mayo Hospital, Lahore from October 2019 to September 2020. Patients were HIV-contaminated smokers at any rate 40 years, in any event 23 pack years, with a CD4 þ T-lymphocyte nadir check under 360 cells/ml. Our current research was conducted at Jinnah Hospital, Lahore from May 2019 to April 2020. Single chest CT with a proposed normalized workup calculation of positive pictures. Fundamental result measure: The result was the quantity of histologically demonstrated lung malignancies determined by CT to have a 2-year development. Results: Median age of the 442 included patients was 49.8 years, 81.6% were under 55 a long time, 84% were men, middle smoking was 30 pack-years, middle nadir and last CD4 þ cell tallies were 168 and 574 cells/ml, separately, and 93% of patients had a plasma HIV RNA under 50 duplicates/ml. A positive picture at standard was accounted for in 95 (23%) patients, and 18 (4.5%) patients had 19 intrusive methodologies with no genuine unfriendly occasions. Cellular breakdown in the lungs was analyzed in 12 patients (six at beginning phases), of which nine (3.1%, 96% certainty span: 0.9- 3.9) were CT recognized, and eight in patients under 57 years. Conclusion: Early cellular breakdown in the lung’s analysis with CT in HIV-contaminated smokers was plausible, safe, and yielded a critical number of tumors. Cellular breakdown in the lungs screening of HIV infected smokers with a significant history of immunodeficiency uncovered a considerable number of tumors at more youthful ages than the focused-on reach in everybody","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74865108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Chlordiazepoxide Oral Tablet On Oral Recurrent Bruxism, A Clinical Trial Human Study","authors":"Ghadah Ali Al-Oudah, A. Al-Ameedee, Ali Ameer Al-Ameedee","doi":"10.31838/SRP.2021.1.128","DOIUrl":"https://doi.org/10.31838/SRP.2021.1.128","url":null,"abstract":"Background: Oral recurrent bruxism is the most common disease of the oral cavity. The underlying etiology remains unclear, and no curative treatment is available. Chlordiazepoxide is essential and useful as anti-anxiety, sedative, appetite-stimulating and weak analgesic actions substance, which recent evidence indicates its involved in emotional responses. Objectives: The present study aimed to use systemic drug such as Chlordiazepoxide oral tablet (5mg). Illnesses related to the oral bruxism by studying its effects in oral recurrent bruxism, this systemic therapy is not indicated in such situation among other drugs. Methods: In this study patients presented with oral recurrent bruxism were treated with Chlordiazepoxide oral tablet (5mg) administered orally once daily after night meal . The dispersible tablet was administered orally once daily for 10 days, 66 participants, 7 were excluded due to incomplete records (51 males and 8 females aged 45.0±6 years) with oral recurrent bruxism, divided into two groups; group A, 30 patients were randomly assigned to receive Chlordiazepoxide oral tablet (5mg) once per day at night, and group B; 29 patients with oral placebo daily for 10 days. Results: The results showed that administering of Chlordiazepoxide oral tablet (5mg) accelerated the healing process within a short time period (10 days) without complications or disfigurement in all patients. Group A, 24 patients, (the healing rate were 0.79%) of 30 patients were used Chlordiazepoxide oral tablet (5mg) doses administered orally had complete healing of oral recurrent bruxism at period time eight days of enzyme‑linked immunosorbent assay investigation. Also there will be a decline both in salivary cortisol and salivary alpha-amylase (sAA)levels during the time period of the study, compared with group B, only 6 patients (the healing rate were 0.21%) of 29 placebo-randomized patients. Conclusion: In this study showed that, the Chlordiazepoxide oral tablet treatment was effective in healing the oral recurrent bruxism and the end-points of the study were complete healing and absence of any discomfort pain while eating within a short period of treatment.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73832273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical Literacy-Based Instruction In Teaching Reading: Insights And Challenges","authors":"L. Hakim, Sadieli Telaumbanua, Nurul Farida, Muhamad Syamsul Taufik, Marfu’ah Laswaniyah, Bayu Pasupati","doi":"10.31838/SRP.2021.3.14","DOIUrl":"https://doi.org/10.31838/SRP.2021.3.14","url":null,"abstract":"Teaching reading in the Indonesian educational system still focuses only on the comprehension and vocabulary exercises and does not encourage learners to become critical thinkers in reading the text. Critical literacy-based instruction is a critical thinking development technique that helps students become active meaning-makers. This study investigated teachers’ understanding and challenges on critical literacy-based instruction in teaching reading. In this case study, the research questionnaire and interview were used as instruments of data collection. The data collected from the respondents were gathered together to be analyzed using qualitative analysis of interactive models. The result of this study shows that some teachers were in misconception about critical literacy. They are also difficult to differentiate the concept of Higher Order Thinking Skills (HOTS) and critical literacy. Besides, they face several obstacles and challenges in implementing critical literacy-based instruction in teaching reading. Mostly, when they asked students to evaluate multiple texts to study how the story changes when told from different perspectives, read from a resistant perspective, and produce counter texts. Enhancing students’ critical thinking ability in reading can be approached from three perspectives; critical thinking, critical pedagogy, and critical literacy. This study only focused on critical literacy in teaching reading. This study will be preliminary inputs for the readers or other researchers to further study in a similar research area with different interests.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73892789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Application Of Multi Wall Carbon Nano-Tubes (Mwcnts) On Implant","authors":"Mohammed Kadhim Al-koofee, And Norehan Moktar","doi":"10.31838/SRP.2021.3.26","DOIUrl":"https://doi.org/10.31838/SRP.2021.3.26","url":null,"abstract":"Dental implants are the most predictable therapeutic option for replacement. partial or total missing teeth, however, there may be inherent factors the patient, implants or surgical technique that may increase the risk failure of implant treatment and that should be taken into consideration. The objective of this article is to carry out a review of the current literature with the in order to show the risk factors that can lead to the loss of implants dental and, therefore, the failure of implant treatment. The complexity of treatment with dental implants due to accumulated pathology, medications associated and various factors that may increase the risk of treatment failure should to be valued. By it, is important perform a Right diagnosis with he end of identify factors of risk what may drive to the failure of the implants with he end to achieve reestablish the Health, the esthetic and the function from our patients by Many years.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81652556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Changes in Obesity and Adiposity Measurements, Comprising BMI Waist, Body Fat and Magnitude by Racial Background in Pakistan Amongst Adults","authors":"Aljasia Kalsoom, Salal Ahmad Asifa Zaib, M. Shahid, Masooma Ahmad, R. Jabeen","doi":"10.51268/SRP.2021.6.13","DOIUrl":"https://doi.org/10.51268/SRP.2021.6.13","url":null,"abstract":"Aim: Review of weight and adiposity trends. Assessments of the race or nationality of individuals in Pakistan from 2017 to 2020 include weight lists, Body weight, muscle-to-fat ratio and slenderness-mass. Methods: Professionals with standardized standards have calculated the weight, height, and circumference of the waist of people 20 years or older. Corpulence The weight list for the non-Asians was classified as thirty or above and for the Asians as high as 27.5. The abdominal circumference of the stomach was 105 cm and greater for males, and 90 cm or higher for females. In people aged 20-59 years with double energy, the muscle-to-fat and fit weight ratio was calculated to be X Beam Absorptiometry. Results: There were 22,428 people in this research from NHANES 2011-18. For 22.095 adults and for 21.440 adult’s weight measurement and for 12.966 adults’ muscle-to-fat ratio were acquired. Weight measurement was acquired. The ages-induced penetrating of general obesity in general for the population of 2020- 21 is 36.5% (96% confidence interval 33.6% to 39.4%). The preponderance of gastric blockages has increased by 54.5% (51.2% to 57.95%) and by 44.5% (38.9%, 48.10%) in 2017-18 (P for pattern <0.002). 29.8 (29.3 to 28.2) in 2017-19 to 28.9 (27.3 to 31.5) in 2015-17 (p for trend 0.002), and the age-changed average weight file has been increased by 29.8 (P for trend=2.002). The medial line in 2012-2016 increased from 99.5 to 97.6 cm (99.2 to 104.2 cm) (P for trend=0.02) in 2015-2019. Conclusion: An increasing trend was identified among Pakistani individuals, despite the fact that there was incoherence across race or ethnic assembly, in Corpulence and obesity measurements between 2017-2020.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82110632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health Promoting Lifestyle Behaviors and Sleep Quality among Post-Menopausal Women in Pakistan","authors":"M. Malik, Mehjabin, A. Hussain, Ayisha Hashmi","doi":"10.31838/SRP.2021.3.96","DOIUrl":"https://doi.org/10.31838/SRP.2021.3.96","url":null,"abstract":"Background: One of the most important concerns regarding improving women health is to promote healthy lifestyle behaviors. Adoption of such behaviors can increase the quality of life as well as delay development of co-morbidities due to menopause. Aim: The aim of the study was to assess health promoting behaviors and sleep quality among post-menopausal in Pakistan. Methodology: A descriptive cross-sectional study design was used. Study respondents included postmenopausal women visiting gynecologic clinic of healthcare facilities located in twin cities of Pakistan. Sample size was calculated to be 382 patients to achieve 95% confidence level with 5% margin of error. Two pre-validated structured questionnaires were used for collection of data in this study i.e., Health Promoting Lifestyle Profile II (HPLPII) and Pittsburgh Sleep Quality Index (PSQI). Mann-Whitney and Kruskal-Wallis (p ≥ 0.05) tests were applied according to different demographic variables. Results: The results highlighted that among all the HPLP domains, highest mean score was observed for interpersonal relations (13.53 ± 4.65) followed by nutrition (13.16 ± 4.84) and spiritual growth (11.20 ± 4.43), whereas the lowest score was observed for physical activity (5.57 ± 4.10). The results highlighted that highest scores for PSQI were observed in the domain of sleep disturbances (1.43 ±.588) followed by domain of sleep latency (1.34 ±1.18) and subjective sleep quality (1.07 ±.739). Whereas lowest scores were observed in the domain of habitual sleep efficiency (0.25 ±.464) which indicates better sleep quality in this domain. Conclusion: The result of the present study concluded poor health promoting lifestyle behavior and poor sleep quality among post-menopausal women in Pakistan. There is a need of designing awareness programs and sessions for women in order to provide counseling to women regarding the effects of menopause on body and importance of adopting a physical and healthy lifestyle by healthcare professionals.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79783234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Concentration of Risperidone: Correlation with Clinical Response in Patients with Schizophrenia","authors":"A. Hamed, E. Hamdi, A. Hussein, W. T. Soliman, M. M. Mahmoud","doi":"10.31838/SRP.2021.3.98","DOIUrl":"https://doi.org/10.31838/SRP.2021.3.98","url":null,"abstract":"Background: Determination of plasma concentration of antipsychotic drug may provide a valuable tool in dose adjustment and therapeutic efficacy but studies in this area are limited and controversial. Purpose: To assess the relation between plasma concentration of Risperidone and its active metabolite (Paliperidone) and clinical response in Egyptian patients with schizophrenia. Patients and methods: One hundred inpatients diagnosed with schizophrenia were enrolled in the study. Fifty inpatients (33 males, 17 females, with age between 20- 60) completed the study. Risperidone was given in two fixed dose: 6 mg daily (Group I) and 8 mg daily (Group II) for three months. Positive and Negative Syndrome Scales (PANSS) were used for determination of psychopathological state and High- performance liquid chromatography (HPLC) was used for measurement of plasma concentration. These assessments were performed at 1 and 3 months. Results: A highly significant decrease was found in PANSS subscales and total score at 1 and 3 months (P 0.05). Conclusion: Measuring plasma concentration of Risperidone is not useful to enhance efficacy of treatment at 1 or 3 months. More research is required for assessment of other factors affecting the efficacy of Risperidone e.g., receptor occupancy and pharmacogenetics.","PeriodicalId":22121,"journal":{"name":"Systematic Reviews in Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81724561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}