Juan J Fuentes-Valdés, Katia Soto-Liebe, Danilo Pérez-Pantoja, Javier Tamames, Lucy Belmar, Carlos Pedrós-Alió, Daniel Garrido, Mónica Vásquez
{"title":"Draft genome sequences of <i>Cylindrospermopsis raciborskii</i> strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay.","authors":"Juan J Fuentes-Valdés, Katia Soto-Liebe, Danilo Pérez-Pantoja, Javier Tamames, Lucy Belmar, Carlos Pedrós-Alió, Daniel Garrido, Mónica Vásquez","doi":"10.1186/s40793-018-0323-1","DOIUrl":"10.1186/s40793-018-0323-1","url":null,"abstract":"<p><p>Members of the genus <i>Cylindrospermopsis</i> represent an important environmental and health concern. Strains CS-508 and MVCC14 of <i>C. raciborskii</i> were isolated from freshwater reservoirs located in Australia and Uruguay, respectively. While CS-508 has been reported as non-toxic, MVCC14 is a saxitoxin (STX) producer. We annotated the draft genomes of these <i>C. raciborskii</i> strains using the assembly of reads obtained from Illumina MiSeq sequencing. The final assemblies resulted in genome sizes close to 3.6 Mbp for both strains and included 3202 ORFs for CS-508 (in 163 contigs) and 3560 ORFs for MVCC14 (in 99 contigs). Finally, both the average nucleotide identity (ANI) and the similarity of gene content indicate that these two genomes should be considered as strains of the <i>C. raciborskii</i> species.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"26"},"PeriodicalIF":0.0,"publicationDate":"2018-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-018-0323-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36604253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Denis S Grouzdev, Maria S Rysina, Irina A Bryantseva, Vladimir M Gorlenko, Vasil A Gaisin
{"title":"Draft genome sequences of '<i>Candidatus</i> Chloroploca asiatica' and '<i>Candidatus</i> Viridilinea mediisalina', candidate representatives of the <i>Chloroflexales</i> order: phylogenetic and taxonomic implications.","authors":"Denis S Grouzdev, Maria S Rysina, Irina A Bryantseva, Vladimir M Gorlenko, Vasil A Gaisin","doi":"10.1186/s40793-018-0329-8","DOIUrl":"10.1186/s40793-018-0329-8","url":null,"abstract":"<p><p>'<i>Candidatus</i> Chloroploca asiatica' B7-9 and '<i>Candidatus</i> Viridilinea mediisalina' Kir15-3F are mesophilic filamentous anoxygenic phototrophic bacteria from alkaline aquatic environments. Both bacteria became available in the last few years and only in stable enrichment culture. In this study, we report the draft genomic sequences of '<i>Ca</i>. Chloroploca asiatica' B7-9 and '<i>Ca.</i> Viridilinea mediisalina' Kir15-3F, which were assembled from metagenomes of their cultures with a fold coverage 86.3× and 163.8×, respectively. The B7-9 (5.8 Mb) and the Kir15-3F (5.6 Mb) draft genome harbors 4818 and 4595 predicted protein-coding genes, respectively. In this article, we analyzed the phylogeny of representatives of the <i>Chloroflexineae</i> suborder in view of the appearance of new genomic data. These data were used for the revision of earlier published group-specific conserved signature indels and for searching for novel signatures for taxons in the <i>Chloroflexineae</i> suborder.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"24"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36598649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed Seghir Daas, Albert Remus R Rosana, Jeella Z Acedo, Malika Douzane, Farida Nateche, Salima Kebbouche-Gana, John C Vederas
{"title":"Insights into the draft genome sequence of bioactives-producing <i>Bacillus thuringiensis</i> DNG9 isolated from Algerian soil-oil slough.","authors":"Mohamed Seghir Daas, Albert Remus R Rosana, Jeella Z Acedo, Malika Douzane, Farida Nateche, Salima Kebbouche-Gana, John C Vederas","doi":"10.1186/s40793-018-0331-1","DOIUrl":"10.1186/s40793-018-0331-1","url":null,"abstract":"<p><p><i>Bacillus thuringiensis</i> is widely used as a bioinsecticide due to its ability to form parasporal crystals containing proteinaceous toxins. It is a member of the <i>Bacillus cereus</i> sensu <i>lato</i>, a group with low genetic diversity but produces several promising antimicrobial compounds. <i>B. thuringiensis</i> DNG9, isolated from an oil-contaminated slough in Algeria, has strong antibacterial, antifungal and biosurfactant properties. Here, we report the 6.06 Mbp draft genome sequence of <i>B. thuringiensis</i> DNG9. The genome encodes several gene inventories for the biosynthesis of bioactive compounds such as zwittermycin A, petrobactin, insecticidal toxins, polyhydroxyalkanoates and multiple bacteriocins. We expect the genome information of strain DNG9 will provide another model system to study pathogenicity against insect pests, plant diseases, and antimicrobial compound mining and comparative phylogenesis among the <i>Bacillus cereus</i> sensu <i>lato</i> group.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36604252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Doglas Parise, Mariana T D Parise, Marcus V C Viana, Adrian V Muñoz-Bucio, Yazmin A Cortés-Pérez, Beatriz Arellano-Reynoso, Efrén Díaz-Aparicio, Fernanda A Dorella, Felipe L Pereira, Alex F Carvalho, Henrique C P Figueiredo, Preetam Ghosh, Debmalya Barh, Anne C P Gomide, Vasco A C Azevedo
{"title":"First genome sequencing and comparative analyses of <i>Corynebacterium pseudotuberculosis</i> strains from Mexico.","authors":"Doglas Parise, Mariana T D Parise, Marcus V C Viana, Adrian V Muñoz-Bucio, Yazmin A Cortés-Pérez, Beatriz Arellano-Reynoso, Efrén Díaz-Aparicio, Fernanda A Dorella, Felipe L Pereira, Alex F Carvalho, Henrique C P Figueiredo, Preetam Ghosh, Debmalya Barh, Anne C P Gomide, Vasco A C Azevedo","doi":"10.1186/s40793-018-0325-z","DOIUrl":"10.1186/s40793-018-0325-z","url":null,"abstract":"<p><p><i>Corynebacterium pseudotuberculosis</i> is a pathogenic bacterium which has been rapidly spreading all over the world, causing economic losses in the agricultural sector and sporadically infecting humans. Six <i>C. pseudotuberculosis</i> strains were isolated from goats, sheep, and horses with distinct abscess locations. For the first time, Mexican genomes of this bacterium were sequenced and studied in silico. All strains were sequenced using Ion Personal Genome Machine sequencer, assembled using Newbler and SPAdes software. The automatic genome annotation was done using the software RAST and in-house scripts for transference, followed by manual curation using Artemis software and BLAST against NCBI and UniProt databases. The six genomes are publicly available in NCBI database. The analysis of nucleotide sequence similarity and the generated phylogenetic tree led to the observation that the Mexican strains are more similar between strains from the same host, but the genetic structure is probably more influenced by transportation of animals between farms than host preference. Also, a putative drug target was predicted and in silico analysis of 46 strains showed two gene clusters capable of differentiating the biovars <i>equi</i> and <i>ovis</i>: Restriction Modification system and CRISPR-Cas cluster.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"21"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36598646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complete genomic sequence of a novel cold-adapted bacterium, <i>Planococcus maritimus</i> Y42, isolated from crude oil-contaminated soil.","authors":"Ruiqi Yang, Guangxiu Liu, Tuo Chen, Wei Zhang, Gaosen Zhang, Sijing Chang","doi":"10.1186/s40793-018-0328-9","DOIUrl":"10.1186/s40793-018-0328-9","url":null,"abstract":"<p><p><i>Planococcus maritimus</i> Y42, isolated from the petroleum-contaminated soil of the Qaidam Basin, can use crude oil as its sole source of carbon and energy at 20 °C. The genome of <i>P. maritimus</i> strain Y42 has been sequenced to provide information on its properties. Genomic analysis shows that the genome of strain Y42 contains one circular DNA chromosome with a size of 3,718,896 bp and a GC content of 48.8%, and three plasmids (329,482; 89,073; and 12,282 bp). Although the strain Y42 did not show a remarkably higher ability in degrading crude oil than other oil-degrading bacteria, the existence of strain Y42 played a significant role to reducing the overall environmental impact as an indigenous oil-degrading bacterium. In addition, genome annotation revealed that strain Y42 has many genes responsible for hydrocarbon degradation. Structural features of the genomes might provide a competitive edge for <i>P. maritimus</i> strain Y42 to survive in oil-polluted environments and be worthy of further study in oil degradation for the recovery of crude oil-polluted environments.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36598648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"One complete and three draft genome sequences of four <i>Brochothrix thermosphacta</i> strains, CD 337, TAP 175, BSAS1 3 and EBP 3070.","authors":"Nassima Illikoud, Christophe Klopp, Alain Roulet, Olivier Bouchez, Nathalie Marsaud, Emmanuel Jaffrès, Monique Zagorec","doi":"10.1186/s40793-018-0333-z","DOIUrl":"10.1186/s40793-018-0333-z","url":null,"abstract":"<p><p><i>Brochothrix thermosphacta</i> is one of the dominant bacterial species associated with spoilage of chilled meat and seafood products through the production of various metabolites responsible for off-odors. However, metabolic pathways leading to meat and seafood spoilage are not all well known. The production of spoiling molecules seems to depend both on strains and on food matrix. Several <i>B. thermosphacta</i> genome sequences have been reported, all issued from meat isolates. Here, we report four genome sequences, one complete and three as drafts. The four <i>B. thermosphacta</i> strains CD 337, TAP 175, BSAS1 3, and EBP 3070 were isolated from different ecological niches (seafood or meat products either spoiled or not and bovine slaughterhouse). These strains known as phenotypically and genetically different were selected to represent intraspecies diversity. CD 337 genome is 2,594,337 bp long, complete and circular, containing 2593 protein coding sequences and 28 RNA genes. TAP 175, BSAS1 3, and EBP 3070 genomes are arranged in 57, 83, and 71 contigs, containing 2515, 2668, and 2611 protein-coding sequences, respectively. These genomes were compared with two other <i>B. thermosphacta</i> complete genome sequences. The main genome content differences between strains are phages, plasmids, restriction/modification systems, and cell surface functions, suggesting a similar metabolic potential but a different niche adaptation capacity.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-018-0333-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36598647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gamaliel López-Leal, Fernanda Cornejo-Granados, Juan Manuel Hurtado-Ramírez, Alfredo Mendoza-Vargas, Adrian Ochoa-Leyva
{"title":"Functional and taxonomic classification of a greenhouse water drain metagenome.","authors":"Gamaliel López-Leal, Fernanda Cornejo-Granados, Juan Manuel Hurtado-Ramírez, Alfredo Mendoza-Vargas, Adrian Ochoa-Leyva","doi":"10.1186/s40793-018-0326-y","DOIUrl":"https://doi.org/10.1186/s40793-018-0326-y","url":null,"abstract":"<p><p>Microbiome sequencing has become the standard procedure in the study of new ecological and human-constructed niches. To our knowledge, this is the first report of a metagenome from the water of a greenhouse drain. We found that the greenhouse is not a diverse niche, mainly dominated by <i>Rhizobiales</i> and Rodobacterales. The analysis of the functions encoded in the metagenome showed enrichment of characteristic features of soil and root-associated bacteria such as ABC-transporters and hydrolase enzymes. Additionally, we found antibiotic resistances genes principally for spectinomycin, tetracycline, and aminoglycosides. This study aimed to identify the bacteria and functional gene composition of a greenhouse water drain sample and also provide a genomic resource to search novel proteins from a previously unexplored niche. All the metagenome proteins and their annotations are available to the scientific community via http://microbiomics.ibt.unam.mx/tools/metagreenhouse/.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"20"},"PeriodicalIF":0.0,"publicationDate":"2018-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-018-0326-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36588141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ji-Hyun Yun, Hojun Sung, Hyun Sik Kim, Euon Jung Tak, Woorim Kang, June-Young Lee, Dong-Wook Hyun, Pil Soo Kim, Jin-Woo Bae
{"title":"Complete genome sequence of the halophile bacterium <i>Kushneria konosiri</i> X49<sup>T</sup>, isolated from salt-fermented <i>Konosirus punctatus</i>.","authors":"Ji-Hyun Yun, Hojun Sung, Hyun Sik Kim, Euon Jung Tak, Woorim Kang, June-Young Lee, Dong-Wook Hyun, Pil Soo Kim, Jin-Woo Bae","doi":"10.1186/s40793-018-0324-0","DOIUrl":"10.1186/s40793-018-0324-0","url":null,"abstract":"<p><p><i>Kushneria konosiri</i> X49<sup>T</sup> is a member of the <i>Halomonadaceae</i> family within the order <i>Oceanospirillales</i> and can be isolated from salt-fermented larval gizzard shad. The genome of <i>K. konosiri</i> X49<sup>T</sup> reported here provides a genetic basis for its halophilic character. Diverse genes were involved in salt-in and -out strategies enabling adaptation of X49<sup>T</sup> to hypersaline environments. Due to resistance to high salt concentrations, genome research of <i>K. konosiri</i> X49<sup>T</sup> will contribute to the improvement of environmental and biotechnological usage by enhancing understanding of the osmotic equilibrium in the cytoplasm. Its genome consists of 3,584,631 bp, with an average G + C content of 59.1%, and 3261 coding sequences, 12 rRNAs, 66 tRNAs, and 8 miscRNAs.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"19"},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-018-0324-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36562651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete genome sequence of the <i>Robinia pseudoacacia</i> L. symbiont <i>Mesorhizobium amorphae</i> CCNWGS0123.","authors":"Xinye Wang, Yantao Luo, Dongying Liu, Jiamei Wang, Shi Wei, Liang Zhao","doi":"10.1186/s40793-018-0321-3","DOIUrl":"https://doi.org/10.1186/s40793-018-0321-3","url":null,"abstract":"<p><p><i>Mesorhizobium amorphae</i> CCNWGS0123 was isolated in 2006, from effective nodules of <i>Robinia pseudoacacia</i> L. grown in lead-zinc mine tailing site, in Gansu Province, China. <i>M. amorphae</i> CCNWGS0123 is an aerobic, Gram-negative, non-spore-forming rod strain. This paper characterized <i>M. amorphae</i> CCNWGS0123 and presents its complete genome sequence information and genome annotation. The 7,374,589 bp long genome which encodes 7136 protein-coding genes and 63 RNA coding genes, contains one chromosome and four plasmids. Moreover, a chromosome with no gaps was assembled.</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"18"},"PeriodicalIF":0.0,"publicationDate":"2018-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-018-0321-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36521485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dagmar Waltemath, Frank T Bergmann, Claudine Chaouiya, Tobias Czauderna, Padraig Gleeson, Carole Goble, Martin Golebiewski, Michael Hucka, Nick Juty, Olga Krebs, Nicolas Le Novère, Huaiyu Mi, Ion I Moraru, Chris J Myers, David Nickerson, Brett G Olivier, Nicolas Rodriguez, Falk Schreiber, Lucian Smith, Fengkai Zhang, Eric Bonnet
{"title":"Correction to: Meeting report from the fourth meeting of the Computational Modeling in Biology Network (COMBINE).","authors":"Dagmar Waltemath, Frank T Bergmann, Claudine Chaouiya, Tobias Czauderna, Padraig Gleeson, Carole Goble, Martin Golebiewski, Michael Hucka, Nick Juty, Olga Krebs, Nicolas Le Novère, Huaiyu Mi, Ion I Moraru, Chris J Myers, David Nickerson, Brett G Olivier, Nicolas Rodriguez, Falk Schreiber, Lucian Smith, Fengkai Zhang, Eric Bonnet","doi":"10.1186/s40793-018-0320-4","DOIUrl":"10.1186/s40793-018-0320-4","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.4056/sigs.5279417.].</p>","PeriodicalId":21965,"journal":{"name":"Standards in Genomic Sciences","volume":"13 ","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2018-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40793-018-0320-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36402249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}