Stem Cell Reviews and Reports最新文献_第9页

Hematopoietic Stem Cell Fates and the Cellular Hierarchy of Mammalian Hematopoiesis: from Transplantation Models to New Insights from in Situ Analyses. 造血干细胞的命运和哺乳动物造血的细胞层次：从移植模型到原位分析的新见解。

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2025-01-01 Epub Date: 2024-09-02 DOI: 10.1007/s12015-024-10782-8

Dania Shaban, Nay Najm, Lucie Droin, Anastasia Nijnik

{"title":"Hematopoietic Stem Cell Fates and the Cellular Hierarchy of Mammalian Hematopoiesis: from Transplantation Models to New Insights from in Situ Analyses.","authors":"Dania Shaban, Nay Najm, Lucie Droin, Anastasia Nijnik","doi":"10.1007/s12015-024-10782-8","DOIUrl":"10.1007/s12015-024-10782-8","url":null,"abstract":"Hematopoiesis is the process that generates the cells of the blood and immune system from hematopoietic stem and progenitor cells (HSPCs) and represents the system with the most rapid cell turnover in a mammalian organism. HSPC differentiation trajectories, their underlying molecular mechanisms, and their dysfunctions in hematologic disorders are the focal research questions of experimental hematology. While HSPC transplantations in murine models are the traditional tool in this research field, recent advances in genome editing and next generation sequencing resulted in the development of many fundamentally new approaches for the analyses of mammalian hematopoiesis in situ and at single cell resolution. The current review will cover many recent developments in this field in murine models, from the bulk lineage tracing studies of HSPC differentiation to the barcoding of individual HSPCs with Cre-recombinase, Sleeping Beauty transposase, or CRISPR/Cas9 tools, to map hematopoietic cell fates, together with their transcriptional and epigenetic states. We also address studies of the clonal dynamics of human hematopoiesis, from the tracing of HSPC clonal behaviours based on viral integration sites in gene therapy patients to the recent analyses of unperturbed human hematopoiesis based on naturally accrued mutations in either nuclear or mitochondrial genomes. Such studies are revolutionizing our understanding of HSPC biology and hematopoiesis both under homeostatic conditions and in the response to various forms of physiological stress, reveal the mechanisms responsible for the decline of hematopoietic function with age, and in the future may advance the understanding and management of the diverse disorders of hematopoiesis.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"28-44"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Microenvironmental Orchestration on Multicellular Lung Alveolar Organoid Development from Human Induced Pluripotent Stem Cells. 微环境协调对人类诱导多能干细胞多细胞肺泡类器官发育的影响

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2025-01-01 Epub Date: 2024-10-17 DOI: 10.1007/s12015-024-10789-1

Vedat Burak Ozan, Huijuan Wang, Akshay Akshay, Deepika Anand, Youssef Hibaoui, Anis Feki, Janine Gote-Schniering, Ali Hashemi Gheinani, Manfred Heller, Anne-Christine Uldry, Sophie Braga Lagache, Amiq Gazdhar, Thomas Geiser

{"title":"Influence of Microenvironmental Orchestration on Multicellular Lung Alveolar Organoid Development from Human Induced Pluripotent Stem Cells.","authors":"Vedat Burak Ozan, Huijuan Wang, Akshay Akshay, Deepika Anand, Youssef Hibaoui, Anis Feki, Janine Gote-Schniering, Ali Hashemi Gheinani, Manfred Heller, Anne-Christine Uldry, Sophie Braga Lagache, Amiq Gazdhar, Thomas Geiser","doi":"10.1007/s12015-024-10789-1","DOIUrl":"10.1007/s12015-024-10789-1","url":null,"abstract":"Induced pluripotent stem cells (iPSCs) have emerged as promising in vitro tools, providing a robust system for disease modelling and facilitating drug screening. Human iPSCs have been successfully differentiated into lung cells and three-dimensional lung spheroids or organoids. The lung is a multicellular complex organ that develops under the symphonic influence of the microenvironment. Here, we hypothesize that the generation of lung organoids in a controlled microenvironment (cmO) (oxygen and pressure) yields multicellular organoids with architectural complexity resembling the lung alveoli. iPSCs were differentiated into mature lung organoids following a stepwise protocol in an oxygen and pressure-controlled microenvironment. The organoids developed in the controlled microenvironment displayed complex alveolar architecture and stained for SFTPC, PDPN, and KRT5, indicating the presence of alveolar epithelial type II and type I cells, as well as basal cells. Moreover, gene and protein expression levels were also increased in the cmO. Furthermore, pathway analysis of proteomics revealed upregulation of lung development-specific pathways in the cmO compared to those growing in normal culture conditions. In summary, by using a controlled microenvironment, we established a complex multicellular lung organoid derived from iPSCs as a novel cellular model to study lung alveolar biology in both lung health and disease.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"254-275"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progesterone receptor is constitutively expressed in induced Pluripotent Stem Cells (iPSCs). 孕酮受体在诱导多能干细胞（iPSC）中呈组成型表达。

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-08-22 DOI: 10.1007/s12015-024-10776-6

Michele Manganelli, Elena Laura Mazzoldi, Rosalba Monica Ferraro, Marinella Pinelli, Marta Parigi, Seyed Ali Mir Aghel, Mattia Bugatti, Ginetta Collo, Gabriele Stocco, William Vermi, Stefania Masneri, Camillo Almici, Luigi Mori, Silvia Giliani

{"title":"Progesterone receptor is constitutively expressed in induced Pluripotent Stem Cells (iPSCs).","authors":"Michele Manganelli, Elena Laura Mazzoldi, Rosalba Monica Ferraro, Marinella Pinelli, Marta Parigi, Seyed Ali Mir Aghel, Mattia Bugatti, Ginetta Collo, Gabriele Stocco, William Vermi, Stefania Masneri, Camillo Almici, Luigi Mori, Silvia Giliani","doi":"10.1007/s12015-024-10776-6","DOIUrl":"10.1007/s12015-024-10776-6","url":null,"abstract":"Induced Pluripotent Stem Cells (iPSCs) are nowadays a common starting point for wide-ranging applications including 3D disease modeling (i.e. organoids) and in future regenerative medicine. Physiological processes like homeostasis, cell differentiation, development and reproduction are tightly regulated by hormones through binding to their transmembrane or nuclear receptors of target cells. Considering their pleiotropic effect, take into account also their expression in an iPSCs-based disease modeling would better recapitulate the molecular events leading to 3D organoid development and disease study. Here we reported the expression pattern of estrogen receptor (ERα) and progesterone receptor (PR) in four different iPSCs, obtained from CD34 + progenitor cells and skin fibroblasts with four different methods. Expression of ERα and PR mRNA were significantly downregulated in iPSCs as well as fibroblasts compared to MCF7 positive control. Immunofluorescence (IF) staining detected only the expression of PR protein in all the different iPSCs cell lines, while ERα was not detectable. By flow cytometry analysis we observed that the ~ 65% of the total population of iPSCs cells expressed only PR, with 100% fold increase compared to HSPCs and fibroblasts, while ERα was not expressed. Our results collectively demonstrated for the first time that the reprogramming of somatic cells into iPSCs leads to the expression of PR receptor.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2303-2317"},"PeriodicalIF":4.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Alleviate Nuclear Pulposus Cells Degeneration Through the miR-145a-5p/USP31/HIF-1α Signaling Pathway. 骨髓间充质干细胞衍生的外泌体通过miR-145a-5p/USP31/HIF-1α信号通路缓解核浆细胞变性

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-08-30 DOI: 10.1007/s12015-024-10781-9

Kang-Kang Su, De-Chen Yu, Xiong-Fei Cao, Pan Li, Le Chang, Xiao-Lei Yu, Zhi-Quan Li, Mo Li

{"title":"Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Alleviate Nuclear Pulposus Cells Degeneration Through the miR-145a-5p/USP31/HIF-1α Signaling Pathway.","authors":"Kang-Kang Su, De-Chen Yu, Xiong-Fei Cao, Pan Li, Le Chang, Xiao-Lei Yu, Zhi-Quan Li, Mo Li","doi":"10.1007/s12015-024-10781-9","DOIUrl":"10.1007/s12015-024-10781-9","url":null,"abstract":"Bone marrow mesenchymal stem cell (BMSC)-derived exosomes possess therapeutic potential against degenerative diseases. This study aimed to investigate the effects of BMSC-derived exosomes on intervertebral disc degeneration (IVDD) and explore the underlying molecular mechanisms. Through transcriptome sequencing and histological analysis, we observed a significant increase in HIF-1α expression in degenerative nucleus pulposus (NP) tissues. The addition of HIF-1α resulted in elevated expression of inflammatory factors IL-1β and IL-6, higher levels of matrix-degrading enzyme MMP13, and lower expression of aggrecan in NP cells. Co-culturing with BMSCs diminished the expression of HIF-1α, MMP13, IL-1β, and IL-6 in degenerative NP cells induced by overload pressure. miRNA chip analysis and PCR validation revealed that miR-145a-5p was the primary miRNA carried by BMSC-derived exosomes. Overexpression of miR-145a-5p was effective in minimizing the expression of HIF-1α, MMP13, IL-1β, and IL-6 in degenerative NP cells. Luciferase reporter assays confirmed USP31 as the target gene of miR-145a-5p, and the regulation of NP cells by BMSC-derived exosomes via miR-145a-5p was dependent on USP31. In conclusion, BMSC-derived exosomes alleviated IVDD through the miR-145a-5p/USP31/HIF-1α signaling pathway, providing valuable insights into the treatment of IVDD.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2268-2282"},"PeriodicalIF":4.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical Therapeutic Efficacy of Extracellular Vesicles Derived from Adipose-Derived Mesenchymal Stromal/Stem Cells in Diabetic Wounds: a Systematic Review and Meta-Analysis. 脂肪间充质干细胞/干细胞衍生的细胞外囊泡对糖尿病伤口的临床前疗效：系统综述和元分析。

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-07-06 DOI: 10.1007/s12015-024-10753-z

Setareh Soltani, Ahora Zahedi, April Joy S Vergara, Marta Noli, Fumie Mitani Soltysik, Flemming Pociot, Reza Yarani

{"title":"Preclinical Therapeutic Efficacy of Extracellular Vesicles Derived from Adipose-Derived Mesenchymal Stromal/Stem Cells in Diabetic Wounds: a Systematic Review and Meta-Analysis.","authors":"Setareh Soltani, Ahora Zahedi, April Joy S Vergara, Marta Noli, Fumie Mitani Soltysik, Flemming Pociot, Reza Yarani","doi":"10.1007/s12015-024-10753-z","DOIUrl":"10.1007/s12015-024-10753-z","url":null,"abstract":"Extracellular vesicles isolated from adipose tissue-derived mesenchymal stromal/stem cells (ADSC-EVs) have demonstrated promising potential in wound healing treatment. To determine the therapeutic efficacy of ADSC-EVs for diabetic wounds in preclinical models, we performed a meta-analysis of available studies. PubMed and Embase were searched (to April 23, 2023). All full-text articles describing the therapeutic application of ADSC-EVs in diabetic wounds were included. Study outcomes were pooled using a random effects meta-analysis, including wound closure, angiogenesis, and collagen deposition. Other outcomes were only discussed descriptively. Seventy unique records were identified from our search; 20 full-text articles were included for qualitative analysis. Twelve studies were eligible for quantitative meta-analysis. The results showed that ADSC-EVs accelerated diabetic wound healing compared to controls with a large effect (standardized mean difference (SMD) 4.22, 95% confidence interval (CI) 3.07 to 5.36). The administration of ADSC-EVs also improved neovascularization (SMD 9.27, 95% CI 4.70 to 13.83) and collagen deposition (SMD 2.19, 95% CI 0.94 to 3.44), with a large effect. The risk of bias was unclear in all included studies. Conclusively, ADSC-EV is an effective treatment for diabetic wounds in preclinical trials, and it appears justified for transfer into the clinical field.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2016-2031"},"PeriodicalIF":4.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Trends of Exosomes Application in Clinical Trials: A Scoping Review. 外泌体在临床试验中应用的全球趋势：范围综述

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-09-28 DOI: 10.1007/s12015-024-10791-7

Maryam Rahnama, Mohammad Heidari, Zahra Poursalehi, Ali Golchin

{"title":"Global Trends of Exosomes Application in Clinical Trials: A Scoping Review.","authors":"Maryam Rahnama, Mohammad Heidari, Zahra Poursalehi, Ali Golchin","doi":"10.1007/s12015-024-10791-7","DOIUrl":"10.1007/s12015-024-10791-7","url":null,"abstract":"Background: Exosomes, nano-sized extracellular vesicles, have emerged as a promising tool for the diagnosis and treatment of various intractable diseases, including chronic wounds and cancers. As our understanding of exosomes continues to grow, their potential as a powerful therapeutic modality in medicine is also expanding. This systematic review aims to examine the progress of exosome-based clinical trials and provide a comprehensive overview of the therapeutic perspectives of exosomes.Methods: This systematic review strictly follows PRISMA guidelines and has been registered in PROSPERO, the International Prospective Register of Systematic Reviews. It encompasses articles from January 2000 to January 2023, sourced from bibliographic databases, with targeted search terms targeting exosome applications in clinical trials. During the screening process, strict inclusion and exclusion criteria were applied, including a focus on clinical trials utilizing different cell-derived exosomes for therapeutic purposes.Results: Among the 522 publications initially identified, only 10 studies met the stringent eligibility criteria after meticulous screening. The selection process involved systematically excluding duplicates and irrelevant articles to provide a transparent overview.Conclusion: According to our systematic review, exosomes have promising applications in a variety of medical fields, including cell-free therapies and drug delivery systems for treating a variety of diseases, especially cancers and chronic wounds. To ensure safety, potency, and broader clinical applications, further optimization of exosome extraction, loading, targeting, and administration is necessary. While cell-based therapeutics are increasingly utilizing exosomes, this field is still in its infancy, and ongoing clinical trials will provide valuable insights into the clinical utility of exosomes.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2165-2193"},"PeriodicalIF":4.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single cell, Label free Characterisation of Human Mesenchymal Stromal cell Stemness and Future Growth Potential by Autofluorescence Multispectral Imaging. 通过自发荧光多光谱成像技术对人间质基质细胞干性和未来生长潜力进行单细胞、无标记表征。

IF 5.4 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-08-27 DOI: 10.1007/s12015-024-10778-4

Jared M Campbell, Abbas Habibalahi, Adnan Agha, Shannon Handley, Aline Knab, Xiaohu Xu, Akanksha Bhargava, Zhilin Lei, Max Mackevicius, Yuan Tian, Saabah B Mahbub, Ayad G Anwer, Stan Gronthos, Sharon Paton, Shane T Grey, Lindsay Wu, Robert B Gilchrist, Ewa M Goldys

{"title":"Single cell, Label free Characterisation of Human Mesenchymal Stromal cell Stemness and Future Growth Potential by Autofluorescence Multispectral Imaging.","authors":"Jared M Campbell, Abbas Habibalahi, Adnan Agha, Shannon Handley, Aline Knab, Xiaohu Xu, Akanksha Bhargava, Zhilin Lei, Max Mackevicius, Yuan Tian, Saabah B Mahbub, Ayad G Anwer, Stan Gronthos, Sharon Paton, Shane T Grey, Lindsay Wu, Robert B Gilchrist, Ewa M Goldys","doi":"10.1007/s12015-024-10778-4","DOIUrl":"10.1007/s12015-024-10778-4","url":null,"abstract":"Aim: To use autofluorescence multispectral imaging (AFMI) to develop a non-invasive assay for the in-depth characterisation of human bone marrow derived mesenchymal stromal cells (hBM-MSCs).Methods: hBM-MSCs were imaged by AFMI on gridded dishes, stained for endpoints of interest (STRO-1 positivity, alkaline phosphatase, beta galactosidase, DNA content) then relocated and results correlated. Intensity, texture and morphological features were used to characterise the colour distribution of regions of interest, and canonical discriminant analysis was used to separate groups. Additionally, hBM-MSC lines were cultured to arrest, with AFMI images taken after each passage to investigate whether an assay could be developed for growth potential.Results: STRO-1 positivity could be predicted with a receiver operator characteristic area under the curve (AUC) of 0.67. For spontaneous differentiation this was 0.66, for entry to the cell-cycle it was 0.77 and for senescence it was 0.77. Growth potential (population doublings remaining) was estimated with an RMSPE = 2.296. The Mean Absolute Error of the final prediction model indicated that growth potential could be predicted with an error of ± 1.86 doublings remaining.Conclusions: This non-invasive methodology enabled the in-depth characterisation of hBM-MSCs from a single assay. This approach is advantageous for clinical applications as well as research and stands out for the characterisation of both present status as well as future behaviour. The use of data from five MSC lines with heterogenous AFMI profiles supports potential generalisability.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2283-2292"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Conventional Treatments: Exploring CAR-T Cell Therapy for Cancer Stem Cell Eradication. 超越传统疗法：探索消除癌症干细胞的 CAR-T 细胞疗法。

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-09-23 DOI: 10.1007/s12015-024-10786-4

Lobna E Rabie, Ahmed A Mohran, Kholoud A Gaber, Nour M Ali, Asmaa M Abd El Naby, Eman A Ghoniem, Basmala A Abd Elmaksod, Ahmed N Abdallah

{"title":"Beyond Conventional Treatments: Exploring CAR-T Cell Therapy for Cancer Stem Cell Eradication.","authors":"Lobna E Rabie, Ahmed A Mohran, Kholoud A Gaber, Nour M Ali, Asmaa M Abd El Naby, Eman A Ghoniem, Basmala A Abd Elmaksod, Ahmed N Abdallah","doi":"10.1007/s12015-024-10786-4","DOIUrl":"10.1007/s12015-024-10786-4","url":null,"abstract":"Background: For decades cancer remained the center of attention in the scientific community as its survival rates are low. Researchers from all around the world wanted to know the core of the problem as to what initiates cancer in a patient and helps with its progression. Many postulations came to light, but Cancer Stem Cells (CSC) was the most appealing and convincing.Main body: In this review, we shed light on a potential solution to the problem by reviewing CAR-T cells (Chimeric antigen receptor T cells). These specialized T cells are designed to detect specific antigens on cancer cells. We analyse the steps of their formation from the collection of T cells from the patient's bloodstream and modifying it to exhibit specific CAR structures on their surfaces, to reinjecting them back and evaluating their efficacy. We thoroughly investigate the structure of the CAR design with improvements across different generations. The focus extends to the unique properties of CSCs as in how targeting specific markers on them can enhance the precision of cancer therapy.Conclusion: Despite the successes, the review discusses the existing limitations and toxicities associated with CAR-derived therapies, highlighting the ongoing need for research and refinement. Looking ahead, we explore proposed strategies aimed at optimizing CAR-T cell therapy to mitigate adverse effects for improved cancer treatments.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2001-2015"},"PeriodicalIF":4.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142295965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Replicative Senescence of Human Chorionic MSCs on their EV-miRNA Profile. 人绒毛膜间充质干细胞复制衰老对其 EV-miRNA 图谱的影响

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-09-21 DOI: 10.1007/s12015-024-10790-8

Hedviga Košuthová, Lívia K Fecskeová, Jana Matejová, Lucia Slovinská, Marko Morávek, Zuzana Bártová, Denisa Harvanová

{"title":"Effects of Replicative Senescence of Human Chorionic MSCs on their EV-miRNA Profile.","authors":"Hedviga Košuthová, Lívia K Fecskeová, Jana Matejová, Lucia Slovinská, Marko Morávek, Zuzana Bártová, Denisa Harvanová","doi":"10.1007/s12015-024-10790-8","DOIUrl":"10.1007/s12015-024-10790-8","url":null,"abstract":"Chorionic mesenchymal stromal cells (CHO-MSCs) and their extracellular vesicles (EVs) are becoming increasingly popular, since chorion is ethically harmless and an easily accessible source of MSCs. However, until now there is only a limited number of studies with a thorough characterization of CHO-MSCs derived EVs and their miRNA profile. In this study, we monitored changes in the EV-miRNA profile between early and late passage of human CHO-MSCs. First, senescence of CHO-MSCs was induced by serial passaging and confirmed by morphological changes, shortened telomeres and changes in the expression of selected genes. The expression of MSCs-specific surface markers CD73, CD90, CD105 did not change with increasing passages. Next, EVs and their miRNA profiles were compared between early vs late passage cells. Number of EVs and their size were not significantly changed. Seven of the top 10 most expressed EV-miRNAs were common to both early and late passages. A differential expression study between early and late passages identified 37 significantly differentially expressed EV-miRNAs, out of which 23 were found to be associated with pathways of cellular senescence based on KEGG pathway analysis. A set of 9 miRNAs were identified as the most frequently associated with senescence and/or with the most altered expression between early and late passages, out of which miR-145-5p, miR-335-5p and miR-199b-3p were the most significant downregulated miRNAs in late passages. The most upregulated EV-miRNAs were miR-1307-3p, miR-3615 and miR320b. Targeting these miRNAs in future experiments may prolong the therapeutic potential of CHO-MSCs and their EVs.","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2318-2335"},"PeriodicalIF":4.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142295966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural Tube Organoids: A Novel System to Study Developmental Timing. 神经管有机体：研究发育时间的新系统

IF 4.5 3区医学

Stem Cell Reviews and Reports Pub Date : 2024-11-01 Epub Date: 2024-09-04 DOI: 10.1007/s12015-024-10785-5

Alexa Rabeling, Amy van der Hoven, Nathalie Andersen, Mubeen Goolam

引用次数: 0