Somatic Cell and Molecular Genetics最新文献_第8页

Chromosomal mapping of a skeletal muscle specific LIM-only protein FHL3 to the distal end of the short arm of human chromosome 1. 人类1号染色体短臂远端骨骼肌特异性LIM-only蛋白FHL3的染色体定位

Somatic Cell and Molecular Genetics Pub Date : 1998-05-01 DOI: 10.1023/b:scam.0000007122.03392.4b

S M Lee, S K Tsui, K K Chan, M Kotaka, H Y Li, S S Chim, M M Waye, K P Fung, C Y Lee

引用次数: 20

Br-cAMP induction of apoptosis in synchronized CHO cells. Br-cAMP诱导同步CHO细胞凋亡。

Somatic Cell and Molecular Genetics Pub Date : 1998-05-01 DOI: 10.1023/b:scam.0000007120.17128.e0

L R Gurley, A L Jandacek, J G Valdez, R J Sebring, J A D'Anna, T T Puck

{"title":"Br-cAMP induction of apoptosis in synchronized CHO cells.","authors":"L R Gurley, A L Jandacek, J G Valdez, R J Sebring, J A D'Anna, T T Puck","doi":"10.1023/b:scam.0000007120.17128.e0","DOIUrl":"https://doi.org/10.1023/b:scam.0000007120.17128.e0","url":null,"abstract":"The proliferation of suspension cultures of malignant CHO cells was inhibited by 0.5 mM Br-cAMP treatment and restored by its removal. This treatment also inhibited histone H1 phosphorylation completely, reduced histones H2A and H4 phosphorylations, induced DNA degradation, and produced cells containing micronuclei. Agarose gel electrophoresis of the degraded DNA fragments produced a \"ladder\" pattern confirming these cells were undergoing apoptosis. Cell cycle synchrony experiments demonstrated culture growth inhibition was the result of two different cell cycle-specific processes: [1] arrested cell cycle traverse at a restriction point in mid-G1, and [2] rapid apoptosis following cell division. Br-cAMP did not stop cells in late-G1, S, G2, or M from traversing the cell cycle and dividing, but rather, induced apoptosis following mitosis. The restriction point of Br-cAMP arrest was located in the middle of a wider band of G1 arrest induced by isoleucine deprivation. The cells synchronized in G1 before the restriction point were held in G1-arrest by Br-cAMP and spared apoptotic death. These studies support the further study of cAMP derivatives as agents to induce tumor regression by apoptosis and reverse transformation.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007120.17128.e0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21096624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Modified giemsa-11 staining protocol for chromosomes of human and hybrid cells. 改良的giemsa-11染色方法用于人和杂交细胞的染色体。

Somatic Cell and Molecular Genetics Pub Date : 1998-05-01 DOI: 10.1023/b:scam.0000007121.10809.0d

C K Stein

引用次数: 2

Mouse-human somatic cell hybrids: loss of mouse and human chromosomes. 小鼠-人类体细胞杂交:小鼠和人类染色体的缺失。

Somatic Cell and Molecular Genetics Pub Date : 1998-05-01 DOI: 10.1023/b:scam.0000007119.19394.f0

X Wang, M Fox, S Povey, J R Masters

引用次数: 7

Structural organization of the human reduced folate carrier gene: evidence for 5' heterogeneity in lymphoblast mRNA. 人类减少叶酸载体基因的结构组织:淋巴细胞mRNA中5'异质性的证据。

Somatic Cell and Molecular Genetics Pub Date : 1998-05-01 DOI: 10.1023/b:scam.0000007117.50428.63

F M Williams, W F Flintoff

{"title":"Structural organization of the human reduced folate carrier gene: evidence for 5' heterogeneity in lymphoblast mRNA.","authors":"F M Williams, W F Flintoff","doi":"10.1023/b:scam.0000007117.50428.63","DOIUrl":"https://doi.org/10.1023/b:scam.0000007117.50428.63","url":null,"abstract":"The reduced folate carrier (rfc1) gene encodes a protein that is involved in the intracellular accumulation of folates. Point mutations in this gene and alterations resulting in the down regulation of its message are major factors involved in the resistance to antifolate chemotherapeutic compounds. As a framework for understanding the significance of such changes in relation to gene expression and function, in this report we describe the organization of the rfc gene from human lymphoblasts. The gene contains 5 exons (2 to 6) coding for protein. At least four 5' exons, used in a mutually exclusive manner in the production of the rfc message from lymphoblast cells, are spliced to exon 2, which contains the translational start site. \"Semi-quantitative\" PCR indicates that exon 1 is preferentially used. The major transcriptional start site has been mapped by RACE and RNase protection to a region 109 to 135 base pairs 5' to the start of exon 1. The 5' region of the gene has no TATA box-like sequence but contains several consensus binding sites for transcriptional factors such as SP-1, MZF1, CREB, AP-1, ETS, GATA-1 and GATA-2. The overall organization of the human gene is similar to that of the hamster and mouse genes.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007117.50428.63","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21096720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Chromosomal sublocalization of the transcribed human telomere repeat binding factor 2 gene and comparative mapping in the mouse. 人端粒重复结合因子2基因转录的染色体亚定位及其在小鼠中的比较定位。

Somatic Cell and Molecular Genetics Pub Date : 1998-05-01 DOI: 10.1023/b:scam.0000007118.47691.d7

A Y Sakaguchi, S S Padalecki, V Mattern, A Rodriguez, R J Leach, J R McGill, M Chavez, T A Giambernardi

引用次数: 10

Molecular cloning of a zinc finger gene eZNF from a human inner ear cDNA library, and in situ expression pattern of its mouse homologue in mouse inner ear. 人内耳锌指基因eZNF的克隆及其小鼠同源基因在小鼠内耳中的原位表达模式

Somatic Cell and Molecular Genetics Pub Date : 1998-03-01 DOI: 10.1023/b:scam.0000007114.14371.f4

A N Jacob, N A Manjunath, P Bray-Ward, R P Kandpal

{"title":"Molecular cloning of a zinc finger gene eZNF from a human inner ear cDNA library, and in situ expression pattern of its mouse homologue in mouse inner ear.","authors":"A N Jacob, N A Manjunath, P Bray-Ward, R P Kandpal","doi":"10.1023/b:scam.0000007114.14371.f4","DOIUrl":"https://doi.org/10.1023/b:scam.0000007114.14371.f4","url":null,"abstract":"We have isolated and characterized the cDNA for eZNF, a zinc finger gene expressed in human inner ear, from a kinetically enriched human inner ear cDNA library. The sequence of full length cDNA was determined and its expression pattern characterized. A high degree of homology is shared between eZNF and rat transcription factor Kid-1. It belongs to the C2H2 class of zinc finger genes, contains a Kruppel-associated box (KRAB) domain near the N-terminus, and has consensus sites for phosphorylation. The gene is expressed in kidney and inner ear structures of mouse and human as determined by Northern blot analysis. In situ hybridization was used to demonstrate specific expression of the mouse eZNF homologue in epithelial layers of the saccule, semicircular canals, and the cochlea of newborn mice. The genomic clone corresponding to the cDNA was isolated and used for fluorescence in situ hybridization to localize it to human chromosome 5qter. The identification of genes expressed in human inner ear by representational difference analysis, their chromosomal location, and expression pattern of their homologues in developing mouse inner ear comprise a strategy that can potentially identify genes important in hearing and deafness.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007114.14371.f4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20825063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Structural and functional analysis of the protein products derived from mutant fur alleles in an endoprotease-deficient Chinese hamster ovary cell strain. 内源性蛋白酶缺陷中国仓鼠卵巢细胞株等位基因突变蛋白产物的结构和功能分析。

Somatic Cell and Molecular Genetics Pub Date : 1998-03-01 DOI: 10.1023/b:scam.0000007111.46513.70

J F Sucic, M J Spence, T J Moehring

{"title":"Structural and functional analysis of the protein products derived from mutant fur alleles in an endoprotease-deficient Chinese hamster ovary cell strain.","authors":"J F Sucic, M J Spence, T J Moehring","doi":"10.1023/b:scam.0000007111.46513.70","DOIUrl":"https://doi.org/10.1023/b:scam.0000007111.46513.70","url":null,"abstract":"The fur gene encodes the endoprotease, furin. We recently demonstrated mutations in both fur alleles in the mutant Chinese hamster ovary (CHO)-K1 strain, RPE.40, and hypothesized that these mutations were responsible for the endoprotease-deficient phenotype of these cells. We now present the structural and functional properties of three protein products derived from the mutant fur alleles. None of these protein products were able to process the precursor to von Willebrand factor, which is processed by wild-type furin. Pro-protein processing activity initially attributed to one of the mutant proteins was due to wild-type furin produced inadvertently from one of the expression constructs used in these experiments. None of the mutant proteins exhibited evidence of autocatalysis, consistent with the lack of activity versus the test substrate, and glycosylation patterns suggested at least two of them remained in the endoplasmic reticulum. These results confirm that RPE.40 cells are furin null mutants, as earlier evidence had suggested.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007111.46513.70","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20825117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Targeted recombination at the Chinese hamster APRT locus using insertion versus replacement vectors. 利用插入载体和替代载体对中国仓鼠APRT位点进行靶向重组。

Somatic Cell and Molecular Genetics Pub Date : 1998-03-01 DOI: 10.1023/b:scam.0000007112.62928.d8

G M Adair, J B Scheerer, A Brotherman, S McConville, J H Wilson, R S Nairn

{"title":"Targeted recombination at the Chinese hamster APRT locus using insertion versus replacement vectors.","authors":"G M Adair, J B Scheerer, A Brotherman, S McConville, J H Wilson, R S Nairn","doi":"10.1023/b:scam.0000007112.62928.d8","DOIUrl":"https://doi.org/10.1023/b:scam.0000007112.62928.d8","url":null,"abstract":"In this study, we have examined the effects of targeting vector configuration and site of vector linearization on the frequency of targeted recombination at the endogenous CHO APRT locus, and have analyzed the types and class distributions of APRT+ recombinants obtained in APRT targeting experiments employing uncut circular, insertion-type (ends-in), and replacement-type (ends-out) configurations of the same pAG7 targeting vector, including configurations produced by introduction of a double-strand break (DSB) at sites either within, or at the 5' or 3' boundaries of APRT targeting homology. Our results suggest that: 1) plasmid-chromosome targeted recombination in mammalian cells may not be stimulated to the same degree by a DSB in the targeting vector as by a DSB in the chromosomal target; 2) recombinant class distributions are highly dependent upon targeting vector configuration; and 3) one-sided invasion mechanisms may play a significant role in homologous recombination in mammalian cells.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007112.62928.d8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20825118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

A matrix associated region localizes the human SOCS-1 gene to chromosome 16p13.13. 人类SOCS-1基因位于染色体16p13.13的基质相关区域。

Somatic Cell and Molecular Genetics Pub Date : 1998-03-01 DOI: 10.1023/b:scam.0000007115.58601.87

J A Kramer, M D Adams, G B Singh, N A Doggett, S A Krawetz

引用次数: 14