Somatic Cell and Molecular Genetics最新文献_第3页

Polycation-mediated transfection: how to overcome undesirable side effects of sticky DNA complexes. 多阳离子介导转染:如何克服粘性DNA复合物的不良副作用。

Somatic Cell and Molecular Genetics Pub Date : 1999-11-01 DOI: 10.1023/a:1019920516714

A Haberland, R Dallüge, B Erdmann, S Zaitsev, R Cartier, M Schäfer-Korting, M Böttger

引用次数: 0

Role of nuclear background and in vivo environment in variable segregation behavior of the aging-dependent T414G mutation at critical control site for human fibroblast mtDNA replication. 核背景和体内环境在人成纤维细胞mtDNA复制关键控制位点衰老依赖性T414G突变的可变分离行为中的作用

Somatic Cell and Molecular Genetics Pub Date : 1999-11-01 DOI: 10.1023/a:1019972500785

Y Michikawa, K Laderman, K Richter, G Attardi

{"title":"Role of nuclear background and in vivo environment in variable segregation behavior of the aging-dependent T414G mutation at critical control site for human fibroblast mtDNA replication.","authors":"Y Michikawa, K Laderman, K Richter, G Attardi","doi":"10.1023/a:1019972500785","DOIUrl":"https://doi.org/10.1023/a:1019972500785","url":null,"abstract":"Previous work had shown a large accumulation (up to 50% of mtDNA) of a noninherited T414G transversion at a critical control site for mtDNA replication in skin fibroblasts from the majority of human subjects above 65 years old, and its absence in younger individuals. In the present studies, long-term in vitro culture of several fibroblasts populations carrying the heteroplasmic T414G mutation revealed an outgrowth of the mutant cells by wild-type cells. This observation supported the previous conclusion that the mutation accumulation is an in vivo phenomenon, while, at the same time, indicating intrinsic physiological differences between mutant and wild-type cells. Furthermore, subcloning experiments revealed a striking mosaic distribution of the mutation in the original fibroblasts populations, as shown by its presence, in heteroplasmic or homoplasmic form, in a fraction (18-32%) of the fibroblasts, and its absence in the others. In other investigations, transfer of mitochondria from mutation-carrying fibroblasts into mtDNA-less 143B.TK- rho0 206 cells revealed the persistence of the mosaic distribution of the mutation, however, with a near-complete shift to homoplasmy. The generality of the latter phenomenon would exclude a founder effect by one or few mitochondria in the transformation experiments, and would rather point to the important role of the nuclear background in the in vitro behavior of the T414G mutation. The stability of the homoplasmic mutation in rho0 cell transformants provides a powerful tool for analyzing its biochemical effects.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1019972500785","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24652013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Effects of Ape1 overexpression on cellular resistance to DNA-damaging and anticancer agents. Ape1过表达对细胞抗dna损伤和抗癌药物的影响。

Somatic Cell and Molecular Genetics Pub Date : 1999-11-01 DOI: 10.1023/a:1019979613989

L J Schild, K W Brookman, L H Thompson, D M Wilson

{"title":"Effects of Ape1 overexpression on cellular resistance to DNA-damaging and anticancer agents.","authors":"L J Schild, K W Brookman, L H Thompson, D M Wilson","doi":"10.1023/a:1019979613989","DOIUrl":"https://doi.org/10.1023/a:1019979613989","url":null,"abstract":"In vitro biochemical studies indicate that Ape1 is the major mammalian enzyme responsible for repairing abasic lesions in DNA and a significant factor in the processing of specific 3'-replication-blocking termini. Toward addressing the role of Ape1 in cellular resistance to specific DNA-damaging and anticancer agents, we constructed a chinese hamster ovary (CHO) cell line, AA8-Ape1, that exhibits a 7-fold higher Ape1-dependent nuclease activity; this overexpression is abolished upon exposure to tetracycline (Tc). In comparison to the AA8 parental control, our data indicates that Ape1 activity is not rate-limiting for the repair of cytotoxic damages induced by the alkylating agent methyl methanesulfonate (MMS), the oxidizing agent hydrogen peroxide (H2O2), or ionizing radiation (IR). AA8-Ape1 cells did exhibit increased resistance to bleomycin following a chronic 3-day exposure, but not to more acute challenges of 1 h. Most notably, the AA8-Ape1 line displayed approximately 1.7-fold elevated resistance to the replication-blocking nucleoside analog dioxolane cytidine (L-OddC); this improved resistance was abrogated by the addition of Tc to the medium. These studies demonstrate that Ape1 is not rate-limiting in the repair of MMS- or H2O2-induced DNA damage, that Ape1 may dictate the sensitivity of bleomycin, depending on dosing scheme, and for the first time, that Ape1 can influence cellular resistance to the anticancer/antiviral antimetabolite L-OddC.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1019979613989","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24652607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Chemically induced premature chromosome condensation in human fibroblast cell lines: fundamental study for applications to the biodosimetry of local exposure. 化学诱导的人成纤维细胞系过早染色体凝聚:应用于局部暴露生物剂量学的基础研究。

Somatic Cell and Molecular Genetics Pub Date : 1999-11-01 DOI: 10.1023/a:1019968432644

R Kanda, K Eguchi-Kasai, H Itsukaichi, M Mori, I Hayata

{"title":"Chemically induced premature chromosome condensation in human fibroblast cell lines: fundamental study for applications to the biodosimetry of local exposure.","authors":"R Kanda, K Eguchi-Kasai, H Itsukaichi, M Mori, I Hayata","doi":"10.1023/a:1019968432644","DOIUrl":"https://doi.org/10.1023/a:1019968432644","url":null,"abstract":"The premature chromosome condensation (PCC) of human peripheral lymphocytes treated with inhibitors of protein phosphatase has been demonstrated to be an excellent tool for the estimation of high-dose whole-body exposure. To develop a new biodosimetry for local exposure, the cytogenetical reaction of human fibroblast lines to PCC inducers was examined and compared with that of lymphocytes. The efficiency of the induction by calyculin A was greater than that by okadaic acid in both cell types. Calyculin A induced PCC in 5-Gy-irradiated and unirradiated samples at almost the same frequency in the lymphocytes, whereas the efficacy was considerably lower in irradiated fibroblasts than in unirradiated ones. Calcium ionophore enhanced the induction of PCC in irradiated fibroblasts, although PCC frequencies were still much lower than those in the lymphocytes. The frequency of ring chromosomes observed in 2- and 5-Gy-irradiated fibroblasts was too low to be used as a marker for cytogenetic dosimetry, and that of excess fragments, scored as the observed chromosome number minus 46, might be substituted. The frequency of excess fragments for 2-, 5-, and 10-Gy-irradiated fibroblasts was less than 0.75, about 1 and a few per cell, respectively, although these values changed with the culture period. The prospects and limitations of the application of PCC techniques to fibroblasts are discussed.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1019968432644","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24652011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Heteroplasmic segregation associated with trisomy-9 in cultured human cells. 培养的人类细胞中与9三体相关的异质分离。

Somatic Cell and Molecular Genetics Pub Date : 1999-11-01 DOI: 10.1023/a:1019960230827

S K Lehtinen, J N Spelbrink, H T Jacobs

引用次数: 3

Damage-repair kinetics and early adaptive response induced by gamma rays in murine leukocytes in vivo. γ射线诱导小鼠白细胞损伤修复动力学和早期适应性反应。

Somatic Cell and Molecular Genetics Pub Date : 1999-11-01 DOI: 10.1023/a:1019964331735

M T Mendiola-Cruz, P Morales-Ramírez

{"title":"Damage-repair kinetics and early adaptive response induced by gamma rays in murine leukocytes in vivo.","authors":"M T Mendiola-Cruz, P Morales-Ramírez","doi":"10.1023/a:1019964331735","DOIUrl":"https://doi.org/10.1023/a:1019964331735","url":null,"abstract":"The kinetics of damage induction and repair at different doses, and the adaptive response induced by gamma ray exposure were determined in murine leukocytes in vivo. The adaptive response was determined after an adaptive dose of 0.01 Gy and a challenge dose of 1.0 Gy administered 60 min later. DNA damage was measured by the single cell gel electrophoresis. Results indicate there is an early and efficient repair process that acts even during the exposure to radiation, which is able to reduce 80% of damaged cells. Later, an increase in damaged cells occurs, which seems to represent the breaks induced during the repair of other kinds of lesions. This suggests that mouse cells are genetically adapted to repair this kind of damage. It was found that the adaptive pretreatment reduces the percentage of damaged cells caused by the challenge dose to one third, and diminishes the damage produced during the late repair. This indicates that the early adaptive response is caused by the induction of a process that protects DNA from damage induction, i.e., synthesis of substances that scavenge free radicals.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1019964331735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24652611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Removal of UV photoproducts from an adenovirus-encoded reporter gene following infection of unirradiated and UV-irradiated human fibroblasts. 未照射和紫外线照射的人成纤维细胞感染后腺病毒编码的报告基因中紫外线光产物的去除。

Somatic Cell and Molecular Genetics Pub Date : 1999-11-01 DOI: 10.1023/a:1019916415806

I P Boszko, A J Rainbow

{"title":"Removal of UV photoproducts from an adenovirus-encoded reporter gene following infection of unirradiated and UV-irradiated human fibroblasts.","authors":"I P Boszko, A J Rainbow","doi":"10.1023/a:1019916415806","DOIUrl":"https://doi.org/10.1023/a:1019916415806","url":null,"abstract":"Ad5HCMVsp1lacZ is a recombinant nonreplicating adenovirus containing the lacZ gene under the control of the human cytomegalovirus immediate early promoter. Previous reports show that lacZ expression for UV-irradiated Ad5HCMVsp1lacZ is greater in nucleotide excision repair (NER) proficient compared to NER deficient human fibroblasts from patients with xerodermapigmentosum (XP) and Cockaye's syndrome (CS) and that pre-UV-treatment of normal fibroblasts results in an enhanced expression of the lacZ gene from UV-irradiated Ad5HCMVsp1lacZ. We have used a quantitative PCR technique to examine whether UV photoproducts are actually removed from the lacZ gene following infection of human fibroblasts with UV-irradiated Ad5HCMVsp1lacZ. Primers flanking a 2.6-kb region of the lacZ reporter gene were added to equal amounts of DNA extracted from Ad5HCMVsp1lacZ infected cells and amplified by PCR using radiolabelled nucleotides as substrates. Results show a significant removal of UV photoproducts in normal human fibroblasts, but a reduced removal in NER deficient XP and CS cells and an enhanced removal in pre-UV-treated normal fibroblasts.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1019916415806","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24652009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Deregulated Expression of the Keratin 18 Gene in Human Colon Carcinoma Cells 角蛋白18基因在人结肠癌细胞中的失调控表达

Somatic Cell and Molecular Genetics Pub Date : 1999-07-01 DOI: 10.1023/A:1019231926567

Nicole Fossar, Malika Chaouche, Philippe Prochasson, M. Rousset, O. Brison

引用次数: 10

Partial Activation of Gene Activity and Chromatin Remodeling of the Human 14q32.1 Serpin Gene Cluster by HNF-1α and HNF-4 in Fibroblast Microcell Hybrids HNF-1α和HNF-4在成纤维细胞微细胞杂种中部分激活人14q32.1丝氨酸丝氨酸基因簇的活性和染色质重塑

Somatic Cell and Molecular Genetics Pub Date : 1999-07-01 DOI: 10.1023/A:1019279809728

P. Rollini, Lianjun Xu, R. Fournier

引用次数: 6

Brief Communication: Regional Mapping Panels for Human Chromosomes 1, 2, and 7 简要交流:人类染色体1、2和7的区域制图面板

Somatic Cell and Molecular Genetics Pub Date : 1999-07-01 DOI: 10.1023/A:1019236027475

J. Léonard, L. Toji, P. Bender, C. Beiswanger, J. Beck, R. T. Johnson

引用次数: 1