Stamford Journal of Pharmaceutical Sciences最新文献_第2页

Assessment of Analgesic, Cytotoxic and Antioxidant activities of Vallaris solanacea (Roth) Kuntze 荆芥的镇痛、细胞毒和抗氧化活性评价

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8869

U. Karmakar, Dyuti Ghosh, S. K. Sadhu

{"title":"Assessment of Analgesic, Cytotoxic and Antioxidant activities of Vallaris solanacea (Roth) Kuntze","authors":"U. Karmakar, Dyuti Ghosh, S. K. Sadhu","doi":"10.3329/SJPS.V4I1.8869","DOIUrl":"https://doi.org/10.3329/SJPS.V4I1.8869","url":null,"abstract":"The ethanolic extract of leaves and stem of Vallaris solanacea (Roth) Kuntze (Family: Apocynaceae) was screened for its analgesic, cytotoxic and antioxidant activities. Phytochemical analysis of the extract indicated the presence of Reducing Sugar, Tannins, Saponins, Gums, Steroids, Alkaloids, and Glycosides. The ethanolic extract showed statistically significant analgesic activity (p<0.005) in acetic acid induced writhing inhibition in mice at the dose of 500mg/kg body weight and also showed mild effect at the doses of 250mg/kg body weight. In the brine shrimp lethality test, the extract showed cytotoxicity with LC 50 80 μg/ml and LC 90 320 μg/ml. In the qualitative antioxi-dant assay using DPPH (1, 1-diphenyl-2-picryl hydrazyl) the extract showed free radical scavenging properties. These primary findings suggest that the extract might possess some chemical constituents that are responsible for analgesic, cytotoxic and antioxidant activities. Key words : Vallaris solanacea (Roth) Kuntze; phytochemical study; analgesic activity; cytotoxic activity; antioxi-dant activity. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8869 SJPS 2011; 4(1): 64-68","PeriodicalId":21823,"journal":{"name":"Stamford Journal of Pharmaceutical Sciences","volume":"25 1","pages":"64-68"},"PeriodicalIF":0.0,"publicationDate":"2011-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84753848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effect of Proteinase-K on Genomic DNA Extraction from Gram-positive Strains 蛋白酶k对革兰氏阳性菌株基因组DNA提取的影响

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8867

M. Shahriar, R. Haque, Shaila Kabir, Irin Dewan, M. A. Bhuyian

{"title":"Effect of Proteinase-K on Genomic DNA Extraction from Gram-positive Strains","authors":"M. Shahriar, R. Haque, Shaila Kabir, Irin Dewan, M. A. Bhuyian","doi":"10.3329/SJPS.V4I1.8867","DOIUrl":"https://doi.org/10.3329/SJPS.V4I1.8867","url":null,"abstract":"Direct extraction of DNA from natural environment and clinical samples has become a useful alternative for the phylogenetic identification and in situ detection of individual microbial cells without cultivation. In this study, three different Gram positive microorganisms ( B. cereus, B. subtilis, and S. aureus ) were chosen for genomic DNA extraction. High salt SDS (Sodium Dodesyl Sulfate) based extraction method was followed to extract genomic DNA with addition of three different lysis protocols to observe the effect of proteinase-K on total genomic DNA yield, lysis steps were carried with SDS, SDS with 3 μl proteinase-K and SDS with 6μl proteinase-K. High molecular weight intact DNA bands were observed only for Bacillus subtilis when the extraction procedure was carried out in presence of SDS, SDS with proteinase-K (3μl) and SDS with increased amount of proteinase-K (6μl). In presence of SDS and increased amount of proteinase-K (6μl) the mean value of DNA concentration for Bacillus cereus , Bacillus subtilis , and Staphylococcus aureus were found to be 1.53±0.15, 1.36±0.10 and 1.65±0.10 μg/μl respectively. However, in absence of proteinase-K, the mean values of DNA concentration were found to be decreased (1.28±0.10, 1.34±0.15, 1.23±0.10 μg/μl for B. cereus, B. subtilis , and S. aureus respectively) for all these stains. Although in case of B. subtilis the overall effect of proteinase-K was not found to be significant in terms of DNA concentration and DNA band intensity, however, for B. cereus , and S. aureus sharp decrease in total extracted DNA concentration was observed suggesting the increased lysis effect of proteinase-K on the thick peptidoglycan layer of Gram-positive cell wall such as B. cereus , and S. aureus . Key words : Extraction; Genomic DNA; Lysis buffer; Gram positive organism. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8867 SJPS 2011; 4(1): 53-57","PeriodicalId":21823,"journal":{"name":"Stamford Journal of Pharmaceutical Sciences","volume":"29 1","pages":"53-57"},"PeriodicalIF":0.0,"publicationDate":"2011-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81043163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Spherical Agglomeration of Naproxan by Solvent Change Method 溶剂变化法制备萘普康球形团聚

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8860

P. K. Kulkarni, M. Dixit, Achint Jain

{"title":"Spherical Agglomeration of Naproxan by Solvent Change Method","authors":"P. K. Kulkarni, M. Dixit, Achint Jain","doi":"10.3329/SJPS.V4I1.8860","DOIUrl":"https://doi.org/10.3329/SJPS.V4I1.8860","url":null,"abstract":"Naproxen, an anti-inflammatory drug, exhibits poor water solubility and flow properties. Spherical agglomerates were prepared by solvent change method. Solvent composition for spherical agglomeration was determined by constructing ternary diagram. Crystallization medium used for spherical agglomerates of naproxen consisted of tetra-hydro-furan (good solvent); water (poor solvent); isopropyl acetate (bridging liquid) in the ratio of 29:61:10, respectively. Spherical agglomerates were characterized by differential scanning calorimetry, infrared spectroscopy, X-ray diffractometry and scanning electron microscopy. Micromeritic and dissolution behavior studies were carried out. Process variables such as amount of bridging liquid, stirring time and duration of stirring were optimized. Dissolution profile of the spherical agglomerates was compared with pure sample and recrystallized sample. Spherical agglomerates exhibited decreased crystallinity and improved micromeritic properties. The dissolution of the spherical agglomerates was not improved compared with pure sample because the dissolution of naproxen dependent on particle size or surface area. Key words : Spherical agglomerates; Naproxen; Crystallinity; Dissolution. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8860 SJPS 2011; 4(1): 1-8","PeriodicalId":21823,"journal":{"name":"Stamford Journal of Pharmaceutical Sciences","volume":"43 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2011-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77310860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Development of a Simple, Sensitive and Rapid Quantitative Analytical Method for Lomefloxacin by High Performance Liquid Chromatography 建立一种简便、灵敏、快速的洛美沙星高效液相色谱定量分析方法

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8871

M. S. Amran, M. R. Hossain, F. Amjad, Sania Sultana, M. A. Baki, M. Hossain

{"title":"Development of a Simple, Sensitive and Rapid Quantitative Analytical Method for Lomefloxacin by High Performance Liquid Chromatography","authors":"M. S. Amran, M. R. Hossain, F. Amjad, Sania Sultana, M. A. Baki, M. Hossain","doi":"10.3329/SJPS.V4I1.8871","DOIUrl":"https://doi.org/10.3329/SJPS.V4I1.8871","url":null,"abstract":"An attempt has been made to develop a simple, sensitive and rapid high performance liquid chromatographic (HPLC) method of analysis for lomefloxacin as in pharmaceutical dosage form using 0.025 M phosphoric acid and acetonitrile (80:20) as mobile phases. The mobile phase was used as solvents to dissolve lomefloxacin and 0.0122 mg/mL stock solution was prepared. Lomefloxacin solution was scanned with UV-spectrophotometer and the absorption maximum (λmax) was found to be 287 nm. This method was successfully applied to five eye drop dosage forms of lomefloxacin encoded as pp1, pp2, pp3, pp4 and pp5 marketed by five different pharmaceutical companies and the result was found to be satisfactory and reproducible. The method was validated by spiked recovery experiments and shown to be linear for lomefloxacin. The method can be used for routine analysis in both research laboratories, and pharmaceutical and chemical industries to analyze the drugs and chemicals without any interference by the excipients. Key words : Lomefloxacin; HPLC; Analysis; Reproducible. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8871 SJPS 2011; 4(1): 69-73","PeriodicalId":21823,"journal":{"name":"Stamford Journal of Pharmaceutical Sciences","volume":"15 1","pages":"69-73"},"PeriodicalIF":0.0,"publicationDate":"2011-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88584390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Dissolution Profile of Ibuprofen Solid Dispersion Prepared with Cellulosic Polymers and Sugar by Fusion Method 纤维素聚合物与糖融合法制备布洛芬固体分散体的溶解谱

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8864

Nadia Saffoon, Y. Jhanker, N. Huda

引用次数: 13

Preparation and Characterization of Mucoadhesive Microcapsules of Gliclazide with Natural Gums 天然树胶黏附格列齐特微胶囊的制备与表征

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8865

S. K. Mankala, Nishanth Kumar Nagamalli, Ramakrishna Raprla, Rajyalaxmi Kommula

{"title":"Preparation and Characterization of Mucoadhesive Microcapsules of Gliclazide with Natural Gums","authors":"S. K. Mankala, Nishanth Kumar Nagamalli, Ramakrishna Raprla, Rajyalaxmi Kommula","doi":"10.3329/SJPS.V4I1.8865","DOIUrl":"https://doi.org/10.3329/SJPS.V4I1.8865","url":null,"abstract":"Gliclazide is an oral hypoglycemic agent used in management of non-insulin dependent diabetes mellitus. Among people who are suffering from long term disorders, the major were categorized under diabetes so, a dosage form is needed to provide continuous therapy with high margin of safety & such dosage form can be achieved by microencapsulation. Gliclazide microspheres with sodium alginate (coat material, gum kondagogu, gum guar and xanthan gum (mucoadhesive agents) were prepared by orifice-ionic gelation and emulsification ionic gelation techniques varying concentrations (1:0.25, 1:0.5, 1:0.75 and 1:1). Formulations were then evaluated for surface morphology, particle shape, Carr’s index, microencapsulation efficiency, drug release, mucoadhesion studies. Compatibility studies were performed by FTIR, DSC, and XRD techniques and no interactions were found between drug and excepients used. The microspheres were found spherical and free flowing with emulsion ionic gelation technique with a size range 400-600μm. % drug content and encapsulation efficiency found in the range of 55%-68% and, 86.23%-94.46% respectively. All microspheres showed good mucoadhesive property in in-vitro wash of test. In vitro drug release studies showed that the guar gum has more potentiality to retard the drug release compared to other gums and concentrations. Drug release from the microspheres was found slow following zero order release kinetics with non-fickian release mechanism stating release depended on the coat: core ratio and the method employed. The concentration of 1:1 of SA: GG (EMG 4) found suitable for preparing the controlled release formulation of gliclazide stating emulsification gelation technique is the best among followed. Key words : Gliclazide; Natural gums; orifice ionic gelation technique; emulsification ionic gelation technique DOI: http://dx.doi.org/10.3329/sjps.v4i1.8865 SJPS 2011; 4(1): 38-48","PeriodicalId":21823,"journal":{"name":"Stamford Journal of Pharmaceutical Sciences","volume":"92 1","pages":"38-48"},"PeriodicalIF":0.0,"publicationDate":"2011-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82382255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Simultaneous Estimation of Rosuvastatin Calcium and Fenofibrate in Bulk and in Tablet Dosage Form by UV-Spectrophotometry and RP-HPLC 紫外分光光度法和反相高效液相色谱法同时测定散装和片剂瑞舒伐他汀钙和非诺贝特的含量

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8868

A. Karunakaran, Vetsa Subhash, Ramu Chinthala, Jayamaryapan Muthuvijayan

引用次数: 27

Effect of Root Extract Fractions of Kyllinga triceps Rottb on Streptozotocin Induced Diabetic Rats 三头牛根提取物对链脲佐菌素诱导的糖尿病大鼠的影响

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8863

S. Vanapatla, G. Mohan, B. Kumar

{"title":"Effect of Root Extract Fractions of Kyllinga triceps Rottb on Streptozotocin Induced Diabetic Rats","authors":"S. Vanapatla, G. Mohan, B. Kumar","doi":"10.3329/SJPS.V4I1.8863","DOIUrl":"https://doi.org/10.3329/SJPS.V4I1.8863","url":null,"abstract":"The present study was aimed to evaluate the root extract fractions of Kyllinga triceps (KT) for their antidiabetic potential on streptozotocin induced diabetes in neonatal rats. Diabetes was induced by a single intraperitoneal injection of Streptozotocin (90mg/kg) to 48±2h old neonatal rats. Effect of root extract fractions (toluene, ethyl acetate, 1- butanol at 50 &100 mg/kg.) were tested for their antihyperglycemic activity by measuring their fasting blood glucose level in diabetic rats at 0,2,4,6,8,12 & 24 h after the treatment. In sub acute study ethyl acetate fraction of KT (EAKT) was administered daily to diabetic rats orally at a dose of 100mg/kg for 28 days. Body weight of the animals and blood glucose level were observed at weekly interval during the study. Cholesterol, triglycerides, insulin, SGPT, ALP, creatinine and total proteins level in serum were also estimated at the initial and after 28 days of the treatment. As the preliminary investigation conducted in our lab on methanolic extract of the roots of KT had showed significant oral glucose tolerance with 200 mg/kg in normal rats. Oral administration of fractions of the plant significantly reduced the fasting blood glucose level in diabetic rats. Among the fractions, EAKT was found to be more effective. Further, in sub-acute study, EAKT, showed a significant anti diabetic activity by reversal of the altered afore said serum biochemical parameters. The results of the study are substantiating the traditional claim of the roots of Kyllinga triceps in the treatment of diabetes with a scope for development of antidiabetic herbal drug from EAKT. Key words : Antidiabetic activity; Kyllinga triceps; Ethyl acetate fraction; Streptozotocin. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8863 SJPS 2011; 4(1): 25-30","PeriodicalId":21823,"journal":{"name":"Stamford Journal of Pharmaceutical Sciences","volume":"51 1","pages":"25-30"},"PeriodicalIF":0.0,"publicationDate":"2011-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74951628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Formulation and Evaluation of Gastroretentive Dosage Form of Ofloxacin 氧氟沙星胃保留剂型的研制与评价

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8861

P. R. Veerareddy, S. Bajjuri, Krishna Sanka, Raju Jukanti, S. Bandari, Rk Ajmeru, A. Potu

{"title":"Formulation and Evaluation of Gastroretentive Dosage Form of Ofloxacin","authors":"P. R. Veerareddy, S. Bajjuri, Krishna Sanka, Raju Jukanti, S. Bandari, Rk Ajmeru, A. Potu","doi":"10.3329/SJPS.V4I1.8861","DOIUrl":"https://doi.org/10.3329/SJPS.V4I1.8861","url":null,"abstract":"The aim of the present investigation was to develop and evaluate gastroretentive drug delivery tablets (GRDDTs) of ofloxacin using different polymers such as HPMC K4M, HPMC K15M, Polyethylene oxide WSR 303, Carbopol 971P, Xanthan Gum in different ratios for local action in gastric region to eradicate Helicobacter pylori infection. The GRDDTs were prepared by wet granulation method and evaluated for physical characteristics such as hardness, thickness, friability, drug content and floating properties. The optimized formula F4 showed better sustained drug release and which also had good floating properties and fitted best to be Korsmeyer-Peppas model with R 2 value of 0.9848. As the n value for the Korsmeyer- Peppas model was found be less than 0.45 it follows Fickian diffusion mechanism. FT-IR result showed that there is no drug excipient interaction. In vivo radiographic studies were conducted with BaSO4 loaded tablets to examine the increased gastric residence time of the prepared tablets. The study revealed that the tablet remained in the stomach for 300±10min which indicates the increase in the gastric residence time for the effective localized action of the ofloxacin in the treatment of Helicobacter pylori caused peptic ulcer. Key words : Ofloxacin; Helicobacter pylori; gastric residence time; gastroretentive drug delivery system. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8861 SJPS 2011; 4(1): 9-18","PeriodicalId":21823,"journal":{"name":"Stamford Journal of Pharmaceutical Sciences","volume":"2 1","pages":"9-18"},"PeriodicalIF":0.0,"publicationDate":"2011-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87995284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Evaluation of Antioxidant and Antinociceptive Properties of Methanolic Extract of Clerodendrum viscosum Vent. 粘胶藤甲醇提取物抗氧化性和抗伤性的评价。

Stamford Journal of Pharmaceutical Sciences Pub Date : 2011-11-07 DOI: 10.3329/SJPS.V4I1.8873

Md. Mostafizur Rahman, N. N. Rumzhum, Kudrat-E-Khuda Zinna

引用次数: 20