Sexual Plant Reproduction最新文献_第7页

DNA methylation during sexual embryogenesis and implications on the induction of somatic embryogenesis in Castanea sativa Miller. 番麻有性胚胎发生过程中的DNA甲基化及其诱导体细胞胚胎发生的意义。

Sexual Plant Reproduction Pub Date : 2010-12-01 Epub Date: 2010-06-15 DOI: 10.1007/s00497-010-0145-9

M Viejo, R Rodríguez, L Valledor, M Pérez, M J Cañal, R Hasbún

{"title":"DNA methylation during sexual embryogenesis and implications on the induction of somatic embryogenesis in Castanea sativa Miller.","authors":"M Viejo, R Rodríguez, L Valledor, M Pérez, M J Cañal, R Hasbún","doi":"10.1007/s00497-010-0145-9","DOIUrl":"https://doi.org/10.1007/s00497-010-0145-9","url":null,"abstract":"From anthesis to mature seed formation, burrs from cross-pollinated adult Castanea sativa Miller trees were characterized and seven developmental stages defined based on macro and micromorphological traits. In order to get an insight into the involvement of epigenetic mechanisms in sexual embryogenesis and to define somatic embryogenesis induction capability, global DNA methylation and the somatic embryogenic competence were quantified. On cross-pollinated trees once fertilization takes place, at least one ovule per ovary becomes dominant, and transient DNA demethylation occurs coinciding with the start of the sexual embryogenic programme. Unfertilized ovules from the same cluster, which maintain their prior size, increase their methylation level and undergo degeneration. These results were validated using non-cross-pollinated trees and the asynchrony of flower receptivity. When testing in vitro somatic embryogenesis response of isolated dominant ovules and axes from zygotic embryos under cross-pollinated conditions, the highest competence was found for reaching seed maturity. Thus, a \"developmental window\" of somatic embryogenesis in chestnut has been characterized. It includes from fertilization to embryo maturity, and a transient decrease in methylation is necessary after fertilization for the development of the somatic embryogenesis response.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 4","pages":"315-23"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0145-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29059455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Strong post-pollination pre-zygotic isolation between sympatric, food-deceptive Mediterranean orchids. 强授粉后合子前隔离之间的同域，食物欺骗地中海兰花。

Sexual Plant Reproduction Pub Date : 2010-12-01 Epub Date: 2010-03-23 DOI: 10.1007/s00497-010-0138-8

Giuseppe Pellegrino, Francesca Bellusci, Aldo Musacchio

{"title":"Strong post-pollination pre-zygotic isolation between sympatric, food-deceptive Mediterranean orchids.","authors":"Giuseppe Pellegrino, Francesca Bellusci, Aldo Musacchio","doi":"10.1007/s00497-010-0138-8","DOIUrl":"https://doi.org/10.1007/s00497-010-0138-8","url":null,"abstract":"Mediterranean orchids that grow in admixed, co-flowering populations, and frequently show hybrid progenies are interesting to use to study the nature and the strength of post-zygotic barriers. However, examination of pre- and post-pollination pre-zygotic isolating mechanisms requires sympatric, co-flowering species pairs that do not produce hybrid swarms. In this study, we analyzed a contact zone between Orchis italica and O. papilionacea, in which hybrid forms have never been reported, although hybridization between members of their groups of appurtenance has been signaled. We investigated pre-pollination barriers observing the floral phenology of both species and identified pollinators by means of molecular analysis of pollinaria collected on the insects captured in the study site. Post-pollination barriers were tested performing manual crosses in order to evaluate pollen germination/pollen tube growth in vivo and fruit and seed formation. Floral phenologies of O. italica and O. papilionacea display nearly overlapping trends, and two common pollinators have been identified by molecular analysis of pollinaria. Thus, pre-pollination barriers are very weak or nonexistent. Bidirectional crosses have shown that the growth of heterospecific pollen tubes is fully blocked in stigmatic cell layers. Since no fruit formation was detected in bidirectional interspecific crosses, we assume that reproductive isolation between the examined species is fully guaranteed by post-pollination pre-zygotic mechanisms acting at stigmatic level. Such condition has been rarely described and may mask the potential action of post-zygotic mechanisms.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 4","pages":"281-9"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0138-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28849083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Pollen performance of Raphanus sativus (Brassicaceae) declines in response to elevated [CO(2)]. 油菜科植物Raphanus sativus的花粉性能随着CO(2)的升高而下降。

Sexual Plant Reproduction Pub Date : 2010-12-01 Epub Date: 2010-06-19 DOI: 10.1007/s00497-010-0146-8

Diane L Marshall, Anna P Tyler, Nathan J Abrahamson, Joy J Avritt, Melanie G Barnes, Leah L Larkin, Juliana S Medeiros, Jerusha Reynolds, Marieken G M Shaner, Heather L Simpson, Satya Maliakal-Witt

{"title":"Pollen performance of Raphanus sativus (Brassicaceae) declines in response to elevated [CO(2)].","authors":"Diane L Marshall, Anna P Tyler, Nathan J Abrahamson, Joy J Avritt, Melanie G Barnes, Leah L Larkin, Juliana S Medeiros, Jerusha Reynolds, Marieken G M Shaner, Heather L Simpson, Satya Maliakal-Witt","doi":"10.1007/s00497-010-0146-8","DOIUrl":"https://doi.org/10.1007/s00497-010-0146-8","url":null,"abstract":"Although increases in atmospheric [CO(2)] are known to affect plant physiology, growth and reproduction, understanding of these effects is limited because most studies of reproductive consequences focus solely on female function. Therefore, we examined the effects of CO(2) enrichment on male function in the annual Raphanus sativus. Pollen donors grown under elevated [CO(2)] initially sired a higher proportion of seeds per fruit than ambient [CO(2)]-grown plants when each was tested against two different standard competitors; however, by the end of the 5-month experiment, these pollen donors sired fewer seeds than ambient [CO(2)]-grown plants and produced a lower proportion of viable pollen grains. The results of this experiment confirm that elevated [CO(2)] can alter reproductive success. Additionally, the change in response to elevated [CO(2)] over time varied among pollen donor families; thus, changes in [CO(2)] could act as a selective force on this species.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 4","pages":"325-36"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0146-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29066495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Breeding behaviour of Kunzea pomifera (Myrtaceae): self-incompatibility, intraspecific and interspecific cross-compatibility. 桃金娘科昆zea pomifera的育种行为:自交不亲和、种内亲和和种间亲和。

Sexual Plant Reproduction Pub Date : 2010-09-01 Epub Date: 2010-02-11 DOI: 10.1007/s00497-010-0133-0

T Page, G M Moore, J Will, G M Halloran

{"title":"Breeding behaviour of Kunzea pomifera (Myrtaceae): self-incompatibility, intraspecific and interspecific cross-compatibility.","authors":"T Page, G M Moore, J Will, G M Halloran","doi":"10.1007/s00497-010-0133-0","DOIUrl":"https://doi.org/10.1007/s00497-010-0133-0","url":null,"abstract":"To examine breeding system characteristics of the endemic Australian prostrate shrub Kunzea pomifera, artificial hybridisations were undertaken using thirteen different genotypes of K. pomifera, to elucidate: (1) self-incompatibility, (2) intraspecific cross-compatibility in the species and (3) interspecific cross-compatibility with each of K. ambigua and K. ericoides. K. pomifera exhibited very low self-compatibility, with the barrier to self-fertilisation being prevention of pollen-tube growth in the style or ovary. Following intraspecific pollination amongst a number of different genotypes of K. pomifera, 38.4% of pollinated flowers developed fruit; arrest of compatible pollen-tubes in the style, preventing fertilisation, contributes to the low fruit set in this species. Interspecific compatibility was examined between K. pomifera (pistillate parent) and K. ambigua (staminate parent) where seed set per pollinated flower (4.47) was not significantly different from intraspecific crosses (4.66). In crosses between K. pomifera (pistillate parent) and K. ericoides as staminate plant, 0.037% of pollinated flowers produced fruit, with 0.0075 seeds per pollinated flower. Reproductive barriers between these two species were evident in the style of K. pomifera, where the growing tips of the K. ericoides pollen-tubes swelled and ceased to grow.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 3","pages":"239-53"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0133-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28707137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Structure of styles and pollen tubes of distylous Turnera joelii and T. scabra (Turneraceae): are there different mechanisms of incompatibility between the morphs? 二瓣蕨和蕨科蕨的花柱和花粉管结构:是否存在不同的不亲和机制?

Sexual Plant Reproduction Pub Date : 2010-09-01 Epub Date: 2010-02-18 DOI: 10.1007/s00497-010-0135-y

D Safavian, J S Shore

{"title":"Structure of styles and pollen tubes of distylous Turnera joelii and T. scabra (Turneraceae): are there different mechanisms of incompatibility between the morphs?","authors":"D Safavian, J S Shore","doi":"10.1007/s00497-010-0135-y","DOIUrl":"https://doi.org/10.1007/s00497-010-0135-y","url":null,"abstract":"We investigate the anatomy and fine structure of styles and pollen tubes of two distylous Turnera species, which possess a heteromorphic self-incompatibility system. We use fluorescence and transmission electron microscopy to provide the first description of the cellular aspects of pollen-pistil interactions and ultrastructural changes to pollen tubes during the self-incompatibility response of the morphs. No obvious ultrastructural differences occur between pistils and compatible pollen tubes. Conspicuous differences were, however, observed between incompatible pollen tubes of the morphs. Incompatible pollen tubes of the long-styled morph always appear to be intact, and pollen tube tips are often highly fluorescent when stained with aniline blue, a stain for callose. Swelling and loss of cristae of mitochondria, and circular rough endoplasmic reticulum, were observed for incompatible pollen tubes of the long-styled morph. For incompatible pollen tubes of the short-styled morph, the tube cell wall apex and plasma membrane often appear to be ruptured and no easily recognizable organelles, such as mitochondria, can be discerned. Our results clearly show ultrastructural differences between the morphs and support the hypothesis that different self-incompatibility mechanisms might operate between them.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 3","pages":"225-37"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0135-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28722032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Location of the stigmatic areas in Mutisia speciosa Aiton ex Hook. a new floral feature in Asteraceae. 木香柱头区位置的研究。菊科花的一个新特征。

Sexual Plant Reproduction Pub Date : 2010-09-01 Epub Date: 2010-04-27 DOI: 10.1007/s00497-010-0141-0

Joseane Bessa, Kelen C Cruz, Milene F Vieira

{"title":"Location of the stigmatic areas in Mutisia speciosa Aiton ex Hook. a new floral feature in Asteraceae.","authors":"Joseane Bessa, Kelen C Cruz, Milene F Vieira","doi":"10.1007/s00497-010-0141-0","DOIUrl":"https://doi.org/10.1007/s00497-010-0141-0","url":null,"abstract":"In Mutisieae species, the style branches are described as short and the stigmatic areas cover the inside of the style branches. As shown in preliminary observations, the Mutisia speciosa florets had long style branches (7 mm), bifurcate only at the apex (about 1.5 mm) and juxtaposed along the remaining length. The objective was to locate the stigmatic areas in M. speciosa. For this purpose, neutral red, 3% hydrogen peroxide and hand pollinations were used. For the pollinations, florets with cut apical portion of the branches (about 2 mm) and florets with intact branches were used. In the first group of florets, the pollen was deposited along the margins of the juxtaposed branches (about 5 mm), and in the second, the pollen was ventrally deposited on the bifurcated apical portion. Some of these florets were left on the plant until fruiting and others were analyzed under a fluorescence microscope to check pollen tube growth. The neutral red test defined two ventro-marginal bands fused at the apex of each style branch, consisting of papillose cells. Intense bubbling in the hydrogen peroxide test showed that only the bands are receptive. The pollinations resulted in fruit sets and growth of pollen tubes, confirming that the bands are receptive along their entire length. This result is new and indicates the need for further studies on the floral biology of tropical Asteraceae species to improve the understanding of their reproductive attributes.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 3","pages":"207-9"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0141-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28948667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Toward in vitro fertilization in Brachiaria spp. 腕毛虫的体外受精研究。

Sexual Plant Reproduction Pub Date : 2010-09-01 Epub Date: 2010-02-05 DOI: 10.1007/s00497-010-0134-z

Diva M A Dusi, Elizangela R Alves, Michiel T M Willemse, Rosana Falcão, Cacilda B do Valle, Vera T C Carneiro

引用次数: 6

Characterisation of sunflower-21 (SF21) genes expressed in pollen and pistil of Senecio squalidus (Asteraceae) and their relationship with other members of the SF21 gene family. 向日葵花粉和雌蕊中表达的SF21基因的特征及其与SF21基因家族其他成员的关系

Sexual Plant Reproduction Pub Date : 2010-09-01 Epub Date: 2010-02-25 DOI: 10.1007/s00497-010-0137-9

Alexandra M Allen, Christian Lexer, Simon J Hiscock

{"title":"Characterisation of sunflower-21 (SF21) genes expressed in pollen and pistil of Senecio squalidus (Asteraceae) and their relationship with other members of the SF21 gene family.","authors":"Alexandra M Allen, Christian Lexer, Simon J Hiscock","doi":"10.1007/s00497-010-0137-9","DOIUrl":"https://doi.org/10.1007/s00497-010-0137-9","url":null,"abstract":"Two related flower-expressed gene copies belonging to the SF21 (sunflower-21) gene family have been isolated from Senecio squalidus (Oxford Ragwort, Asteraceae). These gene copies are differentially expressed in pollen and pistil tissues; ORSF21B (Oxford Ragwort SF21B) is expressed exclusively in mature pollen, whereas ORSF21A (Oxford Ragwort SF21A) is expressed in the transmitting tissue of the style, where it is developmentally regulated. Despite differences in expression, the coding regions of ORSF21A and ORSF21B are highly similar. Amino acid sequence alignments of SF21 genes from a number of angiosperm species indicate that this gene family is conserved in flowering plants and may play an important role in reproductive processes in a wide range of taxa. Phylogenetic analysis of SF21 nucleotide sequence alignments supports this theory, and indicates a complicated history of evolution of this gene family in angiosperms. The putative roles of SF21 genes in reproduction and pollen-pistil interactions are discussed.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 3","pages":"173-86"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0137-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28735721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Tomato pollen respiration in relation to in vitro germination and pollen tube growth under favourable and stress-inducing temperatures. 适宜和胁迫诱导温度下番茄花粉呼吸对离体萌发和花粉管生长的影响。

Sexual Plant Reproduction Pub Date : 2010-09-01 Epub Date: 2010-01-10 DOI: 10.1007/s00497-009-0132-1

I C Karapanos, K A Akoumianakis, C M Olympios, Harold Christopher Passam

{"title":"Tomato pollen respiration in relation to in vitro germination and pollen tube growth under favourable and stress-inducing temperatures.","authors":"I C Karapanos, K A Akoumianakis, C M Olympios, Harold Christopher Passam","doi":"10.1007/s00497-009-0132-1","DOIUrl":"https://doi.org/10.1007/s00497-009-0132-1","url":null,"abstract":"Tomato pollen germination, pollen tube growth and respiratory activity were recorded during incubation in a liquid medium for 7 h over a temperature range of 15-35 degrees C. Although the initial rate of respiration was highest at 30 degrees C, both at 30 degrees C and 35 degrees C respiration decreased after the first hour of incubation due to high temperature impairment of germination and pollen tube growth. The total per cent germination of pollen over the 7-h period was maximal at 15 degrees C whereas pollen tube length was maximal at 25 degrees C. Although the production of CO(2) measured at hourly intervals throughout the incubation period did not correlate to a statistically significant level with either the per cent pollen germination or the length of the pollen tubes alone, nevertheless from 2 h after the start of incubation, it closely correlated with the values for germination x pollen tube length, indicating that the respiratory activity of tomato pollen at a given time is a function of both the per cent germination and the pollen tube growth. We suggest therefore that the rate of respiration might be preferable to a simple germination test for the assessment of pollen germination ability since it expresses not only the pollen germination potential but also the growth vigour of the pollen tubes. In addition, where in vitro tests are designed to assess pollen germination-temperature interactions, they should employ a long incubation period (e.g. 7 h) to permit differences in sensitivity to temperature to be observed.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 3","pages":"219-24"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-009-0132-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28636788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Early germination of Arabidopsis pollen in a double null mutant for the arabinogalactan protein genes AGP6 and AGP11. 阿拉伯半乳聚糖蛋白基因AGP6和AGP11双零突变体中拟南芥花粉的早期萌发

Sexual Plant Reproduction Pub Date : 2010-09-01 Epub Date: 2010-02-17 DOI: 10.1007/s00497-010-0136-x

Sílvia Coimbra, Mário Costa, Marta Adelina Mendes, Ana Marta Pereira, João Pinto, Luís Gustavo Pereira

{"title":"Early germination of Arabidopsis pollen in a double null mutant for the arabinogalactan protein genes AGP6 and AGP11.","authors":"Sílvia Coimbra, Mário Costa, Marta Adelina Mendes, Ana Marta Pereira, João Pinto, Luís Gustavo Pereira","doi":"10.1007/s00497-010-0136-x","DOIUrl":"https://doi.org/10.1007/s00497-010-0136-x","url":null,"abstract":"The pollen specificity of the Arabidopsis arabinogalactan protein (AGP) genes AGP6 and AGP11 suggests that they are integral to pollen biogenesis, and their high percent of sequence similarity may indicate a potential for overlapping function. Arabidopsis agp6 agp11 double null mutants have been studied in our laboratory, and in the present work, we characterize the germination and growth of its pollen. When compared to wild type, mutant agp6 agp11 pollen displayed reduced germination and elongation, both in vivo and in vitro, and precocious germination inside the anthers, provided that sufficient moisture was available. This characteristic was not observed in wild type plants, even in water content conditions which for the mutant were sufficient for pollen germination. Therefore, an additional distinctive phenotypic trait of arabinogalactan proteins AGP6 and AGP11 may be to avert untimely germination of pollen. Such AGPs may control germination through water uptake, suggesting an important biological function of this gene family in pollen.","PeriodicalId":21770,"journal":{"name":"Sexual Plant Reproduction","volume":"23 3","pages":"199-205"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-010-0136-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28718520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61