Frontiers in Bee Science最新文献_第2页

One for all and all for one: a review on the commonality of risk to honeybees and wild pollinators and the benefits of beekeepers in conservation 人人为我，我为人人：蜜蜂和野生授粉昆虫面临的共同风险及养蜂人在保护中的益处综述

Frontiers in Bee Science Pub Date : 2024-03-14 DOI: 10.3389/frbee.2024.1305679

Melissa A. Y. Oddie, Bjørn Dahle

{"title":"One for all and all for one: a review on the commonality of risk to honeybees and wild pollinators and the benefits of beekeepers in conservation","authors":"Melissa A. Y. Oddie, Bjørn Dahle","doi":"10.3389/frbee.2024.1305679","DOIUrl":"https://doi.org/10.3389/frbee.2024.1305679","url":null,"abstract":"Pollinator declines across the globe are centrally driven by a synergistic interaction between intensive land use, pesticides, and climate change. Competition between managed and wild pollinators has been a growing topic of research, however the ensuing social conflict builds antagonism between beekeepers and conservationists, two parties that have an interest in protecting natural diversity for pollinators. The threats perpetuating this potential for competition are as real for managed bees as wild species and uniting both groups, wherever possible, can create long lasting and meaningful change in current agricultural practices. This review examines the most recent literature on pollinator competition and the common threats that drive it. It also delves into the social elements of beekeeping and examines the potential for beekeepers to contribute to the protection of natural habitats. Beekeepers have a genuine interest to preserve natural space and with their charismatic species, dutiful observations, and innovative techniques, they can be valuable assets in filling knowledge gaps and generating public interest. Pollinator strategies in the future should include beekeepers as key stakeholders if their impacts are to be improved.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140243729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplicon and Cas9-targeted nanopore sequencing of Varroa destructor at the onset of an outbreak in Australia 在澳大利亚疫情爆发之初对破坏者 Varroa 进行扩增子和 Cas9 靶向纳米孔测序

Frontiers in Bee Science Pub Date : 2024-02-16 DOI: 10.3389/frbee.2024.1334543

Gus R. Mcfarlane, Katie L. Robinson, Kim Whitaker, John Webster, Lauren Drysdale, Lillian Brancalion, Ashlea Webster, Brendon O’Rourke, Daniel R. Bogema

{"title":"Amplicon and Cas9-targeted nanopore sequencing of Varroa destructor at the onset of an outbreak in Australia","authors":"Gus R. Mcfarlane, Katie L. Robinson, Kim Whitaker, John Webster, Lauren Drysdale, Lillian Brancalion, Ashlea Webster, Brendon O’Rourke, Daniel R. Bogema","doi":"10.3389/frbee.2024.1334543","DOIUrl":"https://doi.org/10.3389/frbee.2024.1334543","url":null,"abstract":"Varroa destructor is a major pest of European honey bees (Apis mellifera), causing significant economic and welfare impacts. Australia remained the last continent free from V. destructor. In June 2022, a detection of V. destructor was reported in sentinel colonies at the Port of Newcastle, Australia. Rapid and accurate identification of the species was critical for timely response and management. In this case study, two Nanopore DNA sequencing methods, PCR amplicon sequencing and Cas9-targeted sequencing, were used to rapidly diagnose the species and mitochondrial haplotype of Varroa mites in parasitized colonies. Nanopore PCR amplicon sequencing provided molecular identification of the species and halogroup determination within 24 hours based on a 458 bp amplicon of the mitochondrial Cytochrome c oxidase subunit I (COXI) gene. We also developed and applied a Cas9-targeted Nanopore sequencing technique that used eight guide RNAs to enrich for 5240 bp of the mitochondrial genome. This method delivered richer data for identification within the same timeframe. Our results underscore the efficacy of Nanopore amplicon sequencing and represent the first reported application of Cas9-targeted Nanopore sequencing within a biosecurity framework. These findings enhance the repertoire of diagnostic tools available for biosecurity applications.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"60 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Warming up through buildings and roads: what we know and should know about the urban heat island effect on bees 通过建筑物和道路升温：关于城市热岛效应对蜜蜂的影响，我们知道什么，应该知道什么

Frontiers in Bee Science Pub Date : 2023-11-28 DOI: 10.3389/frbee.2023.1269600

Carlo Polidori, Andrea Ferrari, Federico Ronchetti, Nicola Tommasi, Elia Nalini

{"title":"Warming up through buildings and roads: what we know and should know about the urban heat island effect on bees","authors":"Carlo Polidori, Andrea Ferrari, Federico Ronchetti, Nicola Tommasi, Elia Nalini","doi":"10.3389/frbee.2023.1269600","DOIUrl":"https://doi.org/10.3389/frbee.2023.1269600","url":null,"abstract":"Urbanization leads to cities having higher temperatures than surrounding non-urban areas [this is known as the urban heat island (UHI) effect]. Very little is known about the impacts of the UHI effect on bees, despite the importance of temperature on many aspects of bees’ life suggesting that these may be not negligible. In this study, we aimed to highlight how the UHI effect could impact relevant functional traits of bees in cities, proposing several ad hoc hypotheses for traits that have thus far been investigated only in few studies or not at all, based on what we know from non-urban studies. The UHI effect was shown to influence bee body size, and generally tended to reduce the body size of bees in cities. Urban temperature may also affect bees’ wing morphology, and thus their overall flight morphology parameters. Individuals may be more brightly colored in cities. Bee ommatidial size and the number of antennal thermoreceptors they have may be smaller and fewer, respectively, in cities than in non-urban areas. As expected, because urban bees face a higher risk of desiccation, higher proportions of alkanes and longer main-carbon chain lengths are expected in their cuticular hydrocarbon (CHC) profiles. Stress biomarkers can also occur at greater concentrations in bees in cities and specific bacteria in the bee gut may occur at lower abundances. Warm urban temperatures may impact the life cycle of pathogens by reducing their proliferation. Aggression levels may be increased, and eusocial species may present more worker phases per year due to the UHI effect. All of these proposed impacts could be likely more visible in solitary and primitively eusocial bee species, which are those suspected to have a more limited dispersal ability. Comparative studies would help in the proper testing of these hypotheses.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"197 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139218430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Honey bee Apis mellifera L. responses to oxidative stress induced by pharmacological and pesticidal compounds 蜜蜂对药理和杀虫剂化合物诱导的氧化应激的反应

Frontiers in Bee Science Pub Date : 2023-11-21 DOI: 10.3389/frbee.2023.1275862

Faizan Tahir, Michael Goblirsch, J. Adamczyk, Shahid Karim, Mohamed Alburaki

{"title":"Honey bee Apis mellifera L. responses to oxidative stress induced by pharmacological and pesticidal compounds","authors":"Faizan Tahir, Michael Goblirsch, J. Adamczyk, Shahid Karim, Mohamed Alburaki","doi":"10.3389/frbee.2023.1275862","DOIUrl":"https://doi.org/10.3389/frbee.2023.1275862","url":null,"abstract":"The western honey bee, Apis mellifera L., is a eusocial insect that plays a significant role in ecosystem balances and the pollination of plants and food crops. Honey bees face multiple biotic and abiotic stressors, such as pathogens, diseases, chemical pesticides, and climate change, which all contribute to honey bee colony loss. This study investigated the impacts of multiple pharmacological and pesticidal molecules on honey bee survival and gene regulation responses. In an 11-day cage experiment, sublethal doses of tunicamycin, thapsigargin, metformin, paraquat, hydrogen peroxide, and imidacloprid were administered to newly emerged sister bees. Daily treatment consumption and mortality were recorded, as well as the transcription expression of 12 major genes (AChE-2, Apisimin, Apidaecin, mrjp1, Sodq, cp450, SelT, SelK, Ire1, Xbp1, Derl-1, and Hsc70), some of which are markers of oxidative and endoplasmic reticulum (ER) stresses in honey bees. At day 9 of the treatments, protein damage was quantified in caged bees. A Kaplan–Meier model indicated significant (p < 0.001) toxicological effects of paraquat, H2O2, and tunicamycin on bee survivorship compared with controls with better survival for other molecules. Post-ingestive aversion responses were recorded only for tunicamycin, hydrogen peroxide, and imidacloprid. Nonetheless, significantly higher protein damage on day 9 was identified only in bees exposed to paraquat and imidacloprid. Some antioxidant genes were significantly regulated vis-à-vis specific treatments. Our results reveal age-related regulation of other major genes with significant inter-gene positive correlations.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"30 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analyses of Asian honeybee from the Sansha Island in the South China Sea, suggest it’s evolutional origin and environmental adaption 对来自中国南海三沙岛的亚洲蜜蜂的基因组分析表明，它的进化起源和环境适应

Frontiers in Bee Science Pub Date : 2023-10-23 DOI: 10.3389/frbee.2023.1144894

Jinglin Gao, Xiangyou Tang, Shan Zhao, Kunlin Tao, Xinyan Shi, Huali Song, Yuxin Yao, Yan Jiang, Tianbin Wang, Xiang Li, Dongxiang Zhao, Jinshan Xu

{"title":"Genomic analyses of Asian honeybee from the Sansha Island in the South China Sea, suggest it’s evolutional origin and environmental adaption","authors":"Jinglin Gao, Xiangyou Tang, Shan Zhao, Kunlin Tao, Xinyan Shi, Huali Song, Yuxin Yao, Yan Jiang, Tianbin Wang, Xiang Li, Dongxiang Zhao, Jinshan Xu","doi":"10.3389/frbee.2023.1144894","DOIUrl":"https://doi.org/10.3389/frbee.2023.1144894","url":null,"abstract":"Discovering new resources and enhancing our knowledge of distribution are crucial for the preservation and utilization of honeybee genetic resources. Our research focused on morphological and population genetic analysis, which revealed significant differences in the SanshaId group compared to others. Notably, the SanshaId group displayed smaller tongue length, averaging at 3.83 mm, and a larger fixation index, indicating a distinct level of subspecies differentiation ( F st = 0.2669). We therefore assumed that A.cerana sanshasis is a new ecotype and subspecies of A. cerana . Estimates of population history indicated that SanshaId population is most closely related to HainanId population, which differed from SanshaId population by about 0.57 Ma due to geological movements. We identified 131 high-frequency non-synonymous mutant genes in the SanshaId group compared to HainanId group. Among these genes, Cuticular genes related to tongue morphology were subject to evolutionary selection, and some genes related to glucose metabolism were highly expressed in the gut. Our results expand the understanding of the distribution range of Asian honeybee and provide a basis for understanding the population dynamics and evolutionary adaptation of A. cerana sanshasis in tropical island environments.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"11 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135366506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adding scent: exploring improvements in pan trapping to monitor pollinators 添加气味:探索利用捕盘法监测传粉媒介的改进

Frontiers in Bee Science Pub Date : 2023-10-17 DOI: 10.3389/frbee.2023.1274989

Madison M. Dunlap, Christina R. Casey, Dustin R. VanOverbeke

{"title":"Adding scent: exploring improvements in pan trapping to monitor pollinators","authors":"Madison M. Dunlap, Christina R. Casey, Dustin R. VanOverbeke","doi":"10.3389/frbee.2023.1274989","DOIUrl":"https://doi.org/10.3389/frbee.2023.1274989","url":null,"abstract":"Our project assessed whether adding floral scents to pan traps would increase the abundance and/or diversity of Hymenoptera sampled or decrease bycatch. We sampled insects using white pan traps containing 0.5 mL of floral scent (linalool, phenylacetaldehyde, limonene, geraniol, benzaldehyde, no scent control) per liter of trapping solution in an interior southern California natural space. Scented traps shared between 53.0% and 79.7% similarity in Hymenoptera assemblage and 59.5% and 72.2% similarity when using presence/absence data as compared with the control. Phenylacetaldehyde and geraniol increased the capture rate of new Hymenoptera, while phenylacetaldehyde also increased overall Hymenoptera and bycatch capture rate. Additionally, we noted that phenylacetaldehyde traps increased small parasitoid wasp capture 10-fold, potentially indicating an alternative use for the addition of phenylacetaldehyde to pan traps. We found no evidence that the addition of scent decreased the abundance or diversity of Hymenoptera caught. Those seeking to exhaustively record the species richness in an area may benefit the most from this method with potential to increase capture of large-bodied, rare, or specialist pollinating Hymenoptera with scent preference, though further research is needed. Based on the data presented here, scent should be explored further for its effect on pan trap catch.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135995091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Varroa destructor and its impacts on honey bee biology 灭螨剂及其对蜜蜂生物学的影响

Frontiers in Bee Science Pub Date : 2023-10-12 DOI: 10.3389/frbee.2023.1272937

Nuria Morfin, Paul H. Goodwin, Ernesto Guzman-Novoa

{"title":"Varroa destructor and its impacts on honey bee biology","authors":"Nuria Morfin, Paul H. Goodwin, Ernesto Guzman-Novoa","doi":"10.3389/frbee.2023.1272937","DOIUrl":"https://doi.org/10.3389/frbee.2023.1272937","url":null,"abstract":"Varroa destructor is considered one of the greatest threats to the health of the honey bee, A. mellifera . In recent years, there has been a considerable increase in the number of studies on the mite and its interaction with the bee at a cellular and molecular level. However, these studies have also revealed just how complex the interaction is. A significant factor in the virulence of V. destructor is the proteins secreted in its saliva, but only a fraction of these have yet been examined. These proteins can negatively affect the bee’s immune system and promote viruses associated with mite parasitism. Initially, studies on parasitized bees concentrated on immune-related genes, but as more genes of the bees have been examined, it is clear that many other aspects of the bee are affected, such as metabolism and neural functioning. Some of those could be responsible for the detrimental changes in certain behaviors of parasitized bees, which compromises the health of the entire colony. Several viruses are associated with V. destructor parasitism, but it remains difficult to distinguish the effects of the viruses from those of the mite. Reduced immunity in parasitized bees also opens the possibility of secondary microbial infections, adding complexity to the mite-bee interaction. Further complicating studies is the impact of other factors, like agrochemicals, which can alter how V. destructor parasitism affects bee immunity, metabolism, and neural functioning. In addition, differences due to age and sex of the bee being parasitized is a factor that needs to be considered in all studies. While much has been learned in recent years about this complex interaction, the number of unanswered questions only increases.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136012909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted crop pollination by training honey bees: advances and perspectives 通过训练蜜蜂进行有针对性的作物授粉:进展和观点

Frontiers in Bee Science Pub Date : 2023-10-11 DOI: 10.3389/frbee.2023.1253157

Walter M. Farina, Andrés Arenas, M. Cecilia Estravis-Barcala, Florencia Palottini

{"title":"Targeted crop pollination by training honey bees: advances and perspectives","authors":"Walter M. Farina, Andrés Arenas, M. Cecilia Estravis-Barcala, Florencia Palottini","doi":"10.3389/frbee.2023.1253157","DOIUrl":"https://doi.org/10.3389/frbee.2023.1253157","url":null,"abstract":"As pollinator-dependent crops continue to expand globally, management strategies are needed to meet the current demand for pollination services. Improving the efficiency of pollinators depends on knowledge about crop plant biology as well as pollinator behavior. In this sense, we will review the scope and challenges of implementing a targeted pollination strategy based on the behavioral individual and social plasticity of the honey bee Apis mellifera. Here we summarize current knowledge on the bees´ ability to perceive, learn and generalize floral odors, the bias of their foraging preferences after in-hive experiences and the transfer of food source information within the social context of the colony, all aspects that impact on foraging decisions and can be used to direct pollinators to target crops. We focused on describing how key olfactory cues that mimic crop floral scents are acquired in the hive and propagate among colony mates to guide foraging to specific crops. Knowledge gaps, including volatiles variability between flowers of the same or different crop varieties, alternative managed pollinators, and potential impact on food industry are discussed.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"243 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136064022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible functions of ecdysone signaling reiteratively used in the adult honey bee brain 蜕皮激素信号在成年蜜蜂脑中反复使用的可能功能

Frontiers in Bee Science Pub Date : 2023-09-27 DOI: 10.3389/frbee.2023.1251714

Yasuhiro Matsumura, Hiroki Kohno, Takeo Kubo

{"title":"Possible functions of ecdysone signaling reiteratively used in the adult honey bee brain","authors":"Yasuhiro Matsumura, Hiroki Kohno, Takeo Kubo","doi":"10.3389/frbee.2023.1251714","DOIUrl":"https://doi.org/10.3389/frbee.2023.1251714","url":null,"abstract":"The European honey bee is a model organism for investigating the molecular and neural bases of the brain underlying social behaviors. Mushroom bodies (MBs) are a higher-order center of memory, learning, and sensory integration in insect brains, and honey bee MBs are a model to study adult neuronal plasticity. In the honey bee, MBs comprise three Class I Kenyon cell (KC) subtypes: large-, middle-, and small-type KCs, which are distinguished based on the size and localization of their somata, and gene expression profiles. One of the unique characteristics of honey bee MBs is that genes for ecdysone signaling are expressed in a spatially and temporarily regulated manner in the adult brain, suggesting that they play a role in the functional specialization of each KC subtype and behavioral control. A recent study reported that the transcription factor Mblk-1/E93, which functions downstream of ecdysone signaling during metamorphosis, targets genes involved in synaptic plasticity underlying memory and learning ability in the adult honey bee brain. On the other hand, the ecdysone receptor (EcR), which is expressed in small-type KCs in the MBs, was reported to target genes involved in lipid metabolism in the brain during foraging flight. The target genes for Mblk-1 and EcR in the adult brains differed from those during metamorphosis, implying that the reiterative use of some transcription factors involved in ecdysone signaling, such as EcR and Mblk-1, has contributed to the acquisition of novel MB functions in Aculeata species, including the honey bee.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"165 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135580458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered synaptic organization in the mushroom bodies of honey bees exposed as foragers to the pesticide fipronil 作为觅食者暴露于杀虫剂氟虫腈的蜜蜂蘑菇体中突触组织的改变

Frontiers in Bee Science Pub Date : 2023-08-11 DOI: 10.3389/frbee.2023.1219991

James J. Privitt, Byron N. Van Nest, S. Fahrbach

{"title":"Altered synaptic organization in the mushroom bodies of honey bees exposed as foragers to the pesticide fipronil","authors":"James J. Privitt, Byron N. Van Nest, S. Fahrbach","doi":"10.3389/frbee.2023.1219991","DOIUrl":"https://doi.org/10.3389/frbee.2023.1219991","url":null,"abstract":"The pesticide fipronil is a GABA receptor antagonist that induces hyperexcitability in the insect nervous system. Its use is controversial because unintentional fipronil exposure may contribute to the ongoing global decline of pollinator populations. Sublethal doses of fipronil have been tentatively linked to reduced colony fitness and impaired learning in the western honey bee, Apis mellifera. The mushroom bodies, insect brain centers important for association learning and memory, contain fipronil-sensitive receptors and may therefore be directly affected by this pesticide. We investigated the synaptic organization of the mushroom bodies in worker honey bees exposed to fipronil using immunolabeling for a pre-synaptic marker and laser scanning confocal microscopy. Exposure of honey bee foragers to fipronil at a feasible field-realistic (1 ppb) concentration decreased the estimated density of immunolabeled microglomerular synaptic complexes in the lip and collar neuropil regions of the mushroom bodies. Effects were also evident after lower (0.1 ppb) and higher (4 ppb) exposures. Other indicators of synaptic organization (bouton number, surface area) were altered by exposure to fipronil in a dose-dependent fashion. These results indicate that sublethal doses of fipronil can result in atypical synaptic organization in the mushroom bodies of honey bees and suggest a mechanism by which fipronil, through perturbation of mushroom body-dependent functions, might diminish honey bee colony survival.","PeriodicalId":217610,"journal":{"name":"Frontiers in Bee Science","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124449388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1