Seed Science Research最新文献

{"title":"Genome-wide association mapping for rapid and uniform germination traits associated with direct-seeded adaptation in rice","authors":"Sasmita Behera, Soumya Priyadarsinee Mohanty, C. Anilkumar, Muhammed Azharudheen T.P., Debashis Moharana, B.C. Marndi, Kutubuddin Ali Molla, A. Anandan, Anjani kumar, Gowda G. Basana, R. Beena, S. Raghu, Lalan Kumar Singh, Rameswar Prasad Sah","doi":"10.1017/s0960258525100032","DOIUrl":"https://doi.org/10.1017/s0960258525100032","url":null,"abstract":"<p>The genetic basis of rapid and uniform seed germination and its associated traits is crucial for improving seed vigour and seedling establishment for higher productivity in direct-seeded rice (DSR) systems. This study investigates the phenotypic diversity and genetic architecture of germination traits in 163 rice genotypes, using a genome-wide association studies (GWAS). An association panel of 163 diverse rice genotypes, including varieties, germplasm and breeding lines, was evaluated for seed germination traits over 2 years (2022 and 2023). The panel was genotyped using 295 simple sequence repeat (SSR) markers, including 80 random SSRs and 215 candidate gene SSRs linked to seed traits and morphological attributes. The genotyping of 163 lines with 295 markers revealed a range of genetic diversity, with polymorphic information content values between 0.04 and 0.93. Population structure analysis indicated the presence of two groups and four sub-groups. GWAS identified 80 significant marker-trait associations (MTAs) across 12 chromosomes at <span>P</span> ≤ 0.05, which narrow down to 18 MTAs at <span>P</span> ≤ 0.01. Twelve candidate genes are identified which were related with multiple traits, linked to important functions, such as seed-size regulation, nutrient mobilization and plant growth. Candidate gene-based SSR (cgSSR) markers such as M169 (OsMIK), M57 (THIS1), M66 (GW2), and M18 (OsBAK1), displayed pleiotropy including rapid and uniform germination (germination index, germination rate index and mean germination time) traits. The newly identified candidate gene markers associated with seed rapid and uniform germination traits can be leveraged in marker-assisted breeding programs to introduce diverse alleles for enhanced seed vigour and crop establishment. Markers closely linked to multiple traits hold significant potential for the simultaneous improvement of several traits.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"72 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A wide range of lens morphologies is associated with breaking physical dormancy in Paraserianthes lophantha subsp. lophantha","authors":"Geoff Burrows","doi":"10.1017/s0960258525100020","DOIUrl":"https://doi.org/10.1017/s0960258525100020","url":null,"abstract":"<p>The classification of acacias has gone through recent upheaval. The latest phylogenies indicate that <span>Acacia sensu stricto</span> is only relatively distantly related to the species with which it was once grouped. Its sister group is the monospecific <span>Paraserianthes</span>. This study concerns <span>P. lophantha</span> subsp. <span>lophantha</span>, a species from SW Western Australia that is widely invasive. Both genera have seeds with physical dormancy (PY) and a lens-type water gap. Seed structure, particularly that of the lens, was assessed in <span>Paraserianthes</span> and compared with <span>Acacia</span>. Seed batch viability was almost 100%, all seeds had PY and average seed mass was 73 mg. The seed coat and the embryo made almost equal contributions to seed mass, indicating a substantial seed coat. Average testa (410 µm) and palisade layer (163 µm) thicknesses were greater than in most investigated <span>Acacia</span> species. Unpopped lenses were small (0.11 mm<span>2</span>, about 0.15% of the seed surface area). With a 1 min boiling water treatment, the lens detached from the seeds. The palisade cells of the lens were about 100% larger in area after detaching, which indicates that they previously were under considerable tension. With other PY-breaking treatments, the lens formed a mound or a slight change in colour occurred. The seeds of <span>Paraserianthes lophantha</span> had the same basic construction as most <span>Acacia</span> seeds, although they were relatively large and heavy, the testa made up a large proportion of the seed and the palisade cells were long. Different lens morphologies, associated with different dormancy-breaking treatments, have rarely been described.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"3 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From oversight to insight: integrating epicotyl emergence to redefine germination and enhance the seed dormancy framework","authors":"Ganesh K. Jaganathan, Shyam S. Phartyal, K.M.G. Gehan Jayasuriya","doi":"10.1017/s0960258525100007","DOIUrl":"https://doi.org/10.1017/s0960258525100007","url":null,"abstract":"<p>The seed science community currently defines germination as radicle emergence of 2 mm from the dispersal unit. Consequently, most seed researchers abruptly terminate germination experiments after radicle emergence, concluding that the seed has germinated. However, this approach underestimates epicotyl dormancy and often leads to dormancy misclassification, or worse, a failure to identify epicotyl dormancy altogether. To address these limitations, we propose extending germination studies to the point of first leaf emergence; we term this the “full germination” period. Our methodology involves germinating fully matured, freshly collected seeds and depending on the time required for radicle emergence, the seeds are categorized into (1) viviparous, where seeds germinate prematurely while they are still attached to the parent plant or within the fruit; (2) Morphological dormancy (MD) or Non-dormant (ND), where seeds germinate within 30 days; and (3) physiological dormancy (PD) and morphophysiological dormancy (MPD), where germination does not occur within 30 days. The absence of shoot emergence within 30 days following radicle protrusion indicates the presence of epicotyl dormancy. Thus, species initially classified as ND, MD, or viviparous may be miscategorized if shoot emergence is not assessed. Likewise, seeds exhibiting PD or MPD may possess an additional epicotyl dormancy component, possibly leading to placing them in incorrect subclass or level. A comprehensive assessment of shoot development is imperative for accurate dormancy characterization. We strongly recommend monitoring seed germination until first true leaf emergence should be adopted to ensure correct conclusions about dormancy, plant life cycles and ecological adaptations.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"46 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vernalization enforces seed dormancy in the agricultural weed Alopecurus myosuroides (Huds.)","authors":"Thomas Edward Holloway, Marta Pérez, Nahema Venceslai, Anne Seville, David Stock, Kazumi Nakabayashi, Gerhard Leubner-Metzger","doi":"10.1017/s0960258525100019","DOIUrl":"https://doi.org/10.1017/s0960258525100019","url":null,"abstract":"Seed dormancy is the key factor determining weed emergence patterns in the field. <jats:italic>Alopecurus myosuroides</jats:italic> (black grass) is a serious cereal weed in Europe that experiences two emergence peaks affecting winter and spring cereals, respectively. Seedlings that emerge in autumn encounter a period of cold winter temperatures, whereas those that emerge in spring do not. In this work, we investigated the effects of this overwintering during vegetative growth on the primary seed dormancy of the offspring. <jats:italic>Alopecurus myosuroides</jats:italic> plants were propagated under controlled conditions where a proportion of the population was subjected to a simulated winter period (vernalization) as seedlings. The offspring produced by vernalized plants was significantly more dormant, requiring longer after-ripening and cold stratification treatments to germinate at warm temperatures. However, there was no difference in the range of temperatures under which dormant seeds germinated. We hypothesized that this difference in dormancy was the result of an epigenetic memory of vernalization. Global changes in DNA methylation of seeds were quantified using an ELISA-based approach. Imbibition in dormant seeds produced by vernalized plants was associated with a global demethylation event that was not observed in the offspring of plants that had not been vernalized. Taken together, these results demonstrate the importance of temperature at different stages of the plant lifecycle in determining dormancy levels and consequently weed emergence patterns in the field.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"143 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed dormancy and germination in Myrtaceae: a palaeohistory, tribe, life cycle and geographical distribution perspective","authors":"Carol C. Baskin, Jerry M. Baskin","doi":"10.1017/s0960258525000066","DOIUrl":"https://doi.org/10.1017/s0960258525000066","url":null,"abstract":"<p>The Myrtaceae is the ninth largest angiosperm family with <span>c</span>. 6000 species, and it diverged from its closest relative the Vochysiaceae <span>c</span>. 100 Ma in southern Gondwana before the final separation of South America and Australia from Antarctica. The family has trees and shrubs and a few viny epiphytes but no herbs and mainly occurs in the tropics and in temperate regions with a Mediterranean climate. Numerous fleshy-fruited species and dry-fruited species have evolved in moist and seasonally dry (fire-prone) regions, respectively. Five kinds of fully developed embryos are found in Myrtaceae seeds, and at maturity seeds are either nondormant (ND) or have physiological dormancy, regardless of embryo morphology, kind of fruit produced, life form, habitat/vegetation region or tribe. Dormant seeds of fleshy-fruited species in wet habitats become ND and germinate at high temperatures. Dormant seeds of dry-fruited species in seasonally dry habitats become ND during the hot, dry season and germinate with the onset of the wet season; seeds germinate only at high temperatures or over a range of low to high temperatures, depending on the species. Seeds of fleshy-fruited species are animal-dispersed, and some Myrteae and Syzygieae are desiccation-sensitive and/or exhibit totipotency. Relatively few species form a persistent soil seed bank, but many dry-fruited species in fire-prone habitats form an aerial seed bank (serotiny). Heat and smoke from fires have a negative, neutral or positive effect on germination, depending on the species. Challenges for maintaining the high species richness of Myrtaceae include habitat destruction/fragmentation, pathogenic fungi and climate change, especially patterns of precipitation.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"1 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed biopriming and long-term air-dry storage effects on Pseudomonas fluorescens viability and Brassica napus germination","authors":"Bernice Mitchener, Joseph King, Carola Peters, Anne Peereboom, Marta Dobrowolska-Haywood, Tina Steinbrecher, Gerhard Leubner-Metzger","doi":"10.1017/s0960258525000054","DOIUrl":"https://doi.org/10.1017/s0960258525000054","url":null,"abstract":"<p>Seed biopriming with <span>Pseudomonas fluorescens</span> as a beneficial microbial inoculant and seed hydropriming with deionized water were conducted with oilseed rape (<span>Brassica napus</span>). Both techniques involve restricted seed hydration followed by seed drying. Seed biopriming reduced the uniformity (time difference between 10 and 90% germination) of germination <span>ca</span> 4-fold, without changing the maximum germination percentages (<span>G</span><span>max</span>) of seed populations. In contrast to this, seed hydropriming improved the uniformity, but not for aged seed populations. The distinct effect of biopriming on germination was caused by the high salt concentration in the priming medium, not by the bacteria or any of the other components. The effects of biopriming duration, seed input and temperature (incubation and drying) were tested and the number of bacteria attached to the seed coat surface was between 1.6 × 10<span>6</span> and 9.8 × 10<span>8</span> colony-forming units (CFUs) per seed. Long-term storage (21°C, &lt;10% relative humidity, 21% oxygen) of dry bioprimed seeds resulted in a rapid decline of bacterial viability, for example (6 h biopriming, 50 g seed input) from 9.8 × 10<span>8</span> CFU per seed to 7.3 × 10<span>4</span> after 4 weeks and 5.0 × 10<span>2</span> after 12 weeks of air-dry seed storage. Seed biopriming and long-term storage of dry bioprimed seeds did not affect <span>G</span><span>max</span> at optimal (24°C) and cold-stress (16°C) temperatures, and did not appreciably affect early seedling growth. Additive biopriming with kimchi paste did not affect the number of bacteria attached per seed but caused an ~800-fold increase in retaining bacterial viability during long-term seed storage.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"41 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed germination ecology of the medicinal plant motherwort (Leonurus cardiaca)","authors":"David J. Susko","doi":"10.1017/s0960258524000254","DOIUrl":"https://doi.org/10.1017/s0960258524000254","url":null,"abstract":"<p><span>Leonurus cardiaca</span> is a perennial mint species with a long history of use as a medicinal herb. It produces a wide variety of phytochemicals with pharmacological properties that are used to treat anxiety and sleep disorders, cardiac disorders, and to reduce inflammation. Surprisingly, scant information is available concerning its seed germination ecology. Hence, this study investigated the presence/kind of seed dormancy and the effects of several environmental factors on seed germination and seedling emergence. Seeds were collected from three populations, and they were subjected to germination and seedling emergence experiments in which environmental factors, including temperature, light, cold stratification, pH, osmotic stress, and depth of burial, were manipulated. Non-stratified seeds germinated over a range of alternating temperature regimes from 20/10 to 30/20°C, but they did not germinate at 15/5°C. Optimum germination occurred between 25/15 and 30/20°C. The presence or absence of light did not affect germination. Cold stratification at 4°C enhanced germination at the two coolest temperature regimes. Seed germination occurred over a solution pH range of 5–10 and exceeded 55% in buffer solutions with pH 6–10. Low levels of osmotic stress reduced germination; only 3–8% of seeds germinated at −0.2 MPa. Maximum seedling emergence occurred when seeds were placed on the soil surface, and emergence decreased with increased burial depths to 5 cm. Overall, seeds exhibited germination characteristics associated with type 2 non-deep physiological dormancy at maturity. Seeds primarily germinated at incubation temperatures of ≥ 25/15°C; however, conditionally dormant seeds became nondormant after prolonged exposure to cold stratification.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"39 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering seed development and germination in the single-cell era","authors":"Paul Schouveiler, Lim Chee Liew, Massimiliano Corso, Loïc Rajjou, Julia Zinsmeister, Mathew G. Lewsey","doi":"10.1017/s0960258524000199","DOIUrl":"https://doi.org/10.1017/s0960258524000199","url":null,"abstract":"<p>Seeds are complex structures that serve as dispersal units in angiosperms. Seeds consist of three specialized tissues with distinct roles and molecular compositions. Hence, the characterization of the genetic regulators that act within individual seed tissues, and how their activity changes during seed development and germination, has been a primary focus of seed research. However, our knowledge of the spatiotemporal modulation of genetic regulators within seeds, across different seed cell types, has been limited by the resolution of available techniques. In the last few years, the development and application of single-cell technologies in plants have enabled the elucidation of gene networks involved in various developmental processes at the cellular level. Some studies have applied these technologies to seeds, enabling further characterization of seed development and germination at the cellular level. Here, we review the current status of the application of single-cell technologies to seeds and present a workflow for conducting single-cell transcriptomics. Additionally, we discuss the integration of single-cell multi-omics, aiming to demonstrate the potential of single-cell technologies in enhancing our comprehension of the spatiotemporal regulations governing seed development and germination.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"84 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed persistence and germination strategies of Carajasia cangae (Rubiaceae): an endemic forb from the Amazon ironstone outcrop","authors":"Diego F. Escobar, Talita Zupo, Carolina da Silva Carvalho, Mauricio T. Coutinho Watanabe, Cecílio F. Caldeira","doi":"10.1017/s0960258525000017","DOIUrl":"https://doi.org/10.1017/s0960258525000017","url":null,"abstract":"<p>Seed persistence, desiccation tolerance, and dormancy play a crucial role in plant population and community dynamics. However, these life-history traits remain largely understudied in perennial herbaceous species, particularly in tropical ecosystems. We evaluated the seed storage behaviour, potential longevity, soil seed bank, seed dormancy alleviation in the field and the effects of after-ripening temperature and time on seed dormancy alleviation in <span>Carajasia cangae</span> – an endangered perennial forb endemic to the ironstone outcrops of the Eastern Amazon. We performed germination experiments to examine the effect of storage conditions (−20, 5 and 28°C, as well as field storage) and time on seed viability, mean germination time and percentage. Our results suggested that <span>C. cangae</span> seeds form a transient soil seed bank and show orthodox storage behaviour. The seeds' longevity was favoured in all controlled storage conditions in relation to soil-stored seeds (field). However, the marked loss of seed viability in less than 1 year, regardless of storage condition, indicates a low potential for long-term germplasm conservation through seed banking. Seed dormancy was fully alleviated after 3 months of field storage during the dry season. Moreover, seeds stored for 6 months at 28°C had their dormancy partially alleviated, indicating that environmental conditions found throughout the dry season in the species habitat are required to alleviate its seed dormancy. A transient seed bank type is favoured by predictable seasonal variations in climate in the region, species iteroparity and seed dormancy alleviation during the dry season, which delays germination until the onset of the next rainy season.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"24 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The oxidative phosphorylation is regulated by ubiquitination under slow-cooling treatment in hydrated lettuce (Lactuca sativa) seeds","authors":"Yingying Han, Wenchao Jiang, Xiuli Wang, Jie Wang, Danping Song, Weidong Yang, Baolin Liu","doi":"10.1017/s0960258525000042","DOIUrl":"https://doi.org/10.1017/s0960258525000042","url":null,"abstract":"<p>The previous study indicated that ubiquitination is involved in the freezing tolerance of hydrated seeds. Parthenolide (PN), inducing the ubiquitination of MDM2, an E3 ring-finger ubiquitin ligase, adversely affects the freezing tolerance of hydrated lettuce seeds. Therefore, a proteomics analysis was conducted to identify PN's targets in hydrated seeds exposed to cooling conditions. Several pathways, including oxidative phosphorylation (KEGG00190), amino sugar and nucleotide sugar metabolism (KEGG00520), and biosynthesis of nucleotide sugars (KEGG01250), were enriched in the PN treatment under slow-cooling conditions (3°C h<span>−1</span>, <span>P</span> &lt; 0.05). Among the proteins in oxidative phosphorylation, the expression of <span>NADH dehydrogenases</span> and <span>ATP synthases</span> (<span>ATPsyn</span>) decreased in PN treatment. In contrast, <span>uncoupling proteins</span> increased after PN treatment, which led to the dissociation of the electron transport chain from ATP synthesis. Treatments with rotenone, dicoumarol, and oligomycin (i.e., oxidative phosphorylation inhibitors) decreased the survival rate of hydrated seeds under freezing conditions, which indicated that energy metabolism was related to the freezing tolerance of hydrated seeds. The predicted interactions between PN and MDM2-like proteins of <span>Lactuca</span> indicated that LsMDM2-5 forms two potential hydrogen bonds with PN. Furthermore, based on AlphaFold predictions and yeast 2-hybrid results, MDM2-5 might interact directly with NADH2. The knockdown of MDM2-5 by RNAi caused a higher level of <span>NADH2</span> and <span>ATPsyn</span> and a higher freezing tolerance of hydrated seeds. This indicated that MDM2 played negative roles in regulating ATP synthesis and freezing tolerance of hydrated seeds.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"55 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}