Seed Science Research最新文献

Seed germination ecology of the medicinal plant motherwort (Leonurus cardiaca)

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-03-04 DOI: 10.1017/s0960258524000254

David J. Susko

{"title":"Seed germination ecology of the medicinal plant motherwort (Leonurus cardiaca)","authors":"David J. Susko","doi":"10.1017/s0960258524000254","DOIUrl":"https://doi.org/10.1017/s0960258524000254","url":null,"abstract":"Leonurus cardiaca is a perennial mint species with a long history of use as a medicinal herb. It produces a wide variety of phytochemicals with pharmacological properties that are used to treat anxiety and sleep disorders, cardiac disorders, and to reduce inflammation. Surprisingly, scant information is available concerning its seed germination ecology. Hence, this study investigated the presence/kind of seed dormancy and the effects of several environmental factors on seed germination and seedling emergence. Seeds were collected from three populations, and they were subjected to germination and seedling emergence experiments in which environmental factors, including temperature, light, cold stratification, pH, osmotic stress, and depth of burial, were manipulated. Non-stratified seeds germinated over a range of alternating temperature regimes from 20/10 to 30/20°C, but they did not germinate at 15/5°C. Optimum germination occurred between 25/15 and 30/20°C. The presence or absence of light did not affect germination. Cold stratification at 4°C enhanced germination at the two coolest temperature regimes. Seed germination occurred over a solution pH range of 5–10 and exceeded 55% in buffer solutions with pH 6–10. Low levels of osmotic stress reduced germination; only 3–8% of seeds germinated at −0.2 MPa. Maximum seedling emergence occurred when seeds were placed on the soil surface, and emergence decreased with increased burial depths to 5 cm. Overall, seeds exhibited germination characteristics associated with type 2 non-deep physiological dormancy at maturity. Seeds primarily germinated at incubation temperatures of ≥ 25/15°C; however, conditionally dormant seeds became nondormant after prolonged exposure to cold stratification.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"39 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering seed development and germination in the single-cell era

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-03-03 DOI: 10.1017/s0960258524000199

Paul Schouveiler, Lim Chee Liew, Massimiliano Corso, Loïc Rajjou, Julia Zinsmeister, Mathew G. Lewsey

{"title":"Deciphering seed development and germination in the single-cell era","authors":"Paul Schouveiler, Lim Chee Liew, Massimiliano Corso, Loïc Rajjou, Julia Zinsmeister, Mathew G. Lewsey","doi":"10.1017/s0960258524000199","DOIUrl":"https://doi.org/10.1017/s0960258524000199","url":null,"abstract":"Seeds are complex structures that serve as dispersal units in angiosperms. Seeds consist of three specialized tissues with distinct roles and molecular compositions. Hence, the characterization of the genetic regulators that act within individual seed tissues, and how their activity changes during seed development and germination, has been a primary focus of seed research. However, our knowledge of the spatiotemporal modulation of genetic regulators within seeds, across different seed cell types, has been limited by the resolution of available techniques. In the last few years, the development and application of single-cell technologies in plants have enabled the elucidation of gene networks involved in various developmental processes at the cellular level. Some studies have applied these technologies to seeds, enabling further characterization of seed development and germination at the cellular level. Here, we review the current status of the application of single-cell technologies to seeds and present a workflow for conducting single-cell transcriptomics. Additionally, we discuss the integration of single-cell multi-omics, aiming to demonstrate the potential of single-cell technologies in enhancing our comprehension of the spatiotemporal regulations governing seed development and germination.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"84 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seed persistence and germination strategies of Carajasia cangae (Rubiaceae): an endemic forb from the Amazon ironstone outcrop

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-02-19 DOI: 10.1017/s0960258525000017

Diego F. Escobar, Talita Zupo, Carolina da Silva Carvalho, Mauricio T. Coutinho Watanabe, Cecílio F. Caldeira

{"title":"Seed persistence and germination strategies of Carajasia cangae (Rubiaceae): an endemic forb from the Amazon ironstone outcrop","authors":"Diego F. Escobar, Talita Zupo, Carolina da Silva Carvalho, Mauricio T. Coutinho Watanabe, Cecílio F. Caldeira","doi":"10.1017/s0960258525000017","DOIUrl":"https://doi.org/10.1017/s0960258525000017","url":null,"abstract":"Seed persistence, desiccation tolerance, and dormancy play a crucial role in plant population and community dynamics. However, these life-history traits remain largely understudied in perennial herbaceous species, particularly in tropical ecosystems. We evaluated the seed storage behaviour, potential longevity, soil seed bank, seed dormancy alleviation in the field and the effects of after-ripening temperature and time on seed dormancy alleviation in Carajasia cangae – an endangered perennial forb endemic to the ironstone outcrops of the Eastern Amazon. We performed germination experiments to examine the effect of storage conditions (−20, 5 and 28°C, as well as field storage) and time on seed viability, mean germination time and percentage. Our results suggested that C. cangae seeds form a transient soil seed bank and show orthodox storage behaviour. The seeds' longevity was favoured in all controlled storage conditions in relation to soil-stored seeds (field). However, the marked loss of seed viability in less than 1 year, regardless of storage condition, indicates a low potential for long-term germplasm conservation through seed banking. Seed dormancy was fully alleviated after 3 months of field storage during the dry season. Moreover, seeds stored for 6 months at 28°C had their dormancy partially alleviated, indicating that environmental conditions found throughout the dry season in the species habitat are required to alleviate its seed dormancy. A transient seed bank type is favoured by predictable seasonal variations in climate in the region, species iteroparity and seed dormancy alleviation during the dry season, which delays germination until the onset of the next rainy season.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"24 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The oxidative phosphorylation is regulated by ubiquitination under slow-cooling treatment in hydrated lettuce (Lactuca sativa) seeds

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-02-06 DOI: 10.1017/s0960258525000042

Yingying Han, Wenchao Jiang, Xiuli Wang, Jie Wang, Danping Song, Weidong Yang, Baolin Liu

{"title":"The oxidative phosphorylation is regulated by ubiquitination under slow-cooling treatment in hydrated lettuce (Lactuca sativa) seeds","authors":"Yingying Han, Wenchao Jiang, Xiuli Wang, Jie Wang, Danping Song, Weidong Yang, Baolin Liu","doi":"10.1017/s0960258525000042","DOIUrl":"https://doi.org/10.1017/s0960258525000042","url":null,"abstract":"The previous study indicated that ubiquitination is involved in the freezing tolerance of hydrated seeds. Parthenolide (PN), inducing the ubiquitination of MDM2, an E3 ring-finger ubiquitin ligase, adversely affects the freezing tolerance of hydrated lettuce seeds. Therefore, a proteomics analysis was conducted to identify PN's targets in hydrated seeds exposed to cooling conditions. Several pathways, including oxidative phosphorylation (KEGG00190), amino sugar and nucleotide sugar metabolism (KEGG00520), and biosynthesis of nucleotide sugars (KEGG01250), were enriched in the PN treatment under slow-cooling conditions (3°C h−1, P < 0.05). Among the proteins in oxidative phosphorylation, the expression of NADH dehydrogenases and ATP synthases (ATPsyn) decreased in PN treatment. In contrast, uncoupling proteins increased after PN treatment, which led to the dissociation of the electron transport chain from ATP synthesis. Treatments with rotenone, dicoumarol, and oligomycin (i.e., oxidative phosphorylation inhibitors) decreased the survival rate of hydrated seeds under freezing conditions, which indicated that energy metabolism was related to the freezing tolerance of hydrated seeds. The predicted interactions between PN and MDM2-like proteins of Lactuca indicated that LsMDM2-5 forms two potential hydrogen bonds with PN. Furthermore, based on AlphaFold predictions and yeast 2-hybrid results, MDM2-5 might interact directly with NADH2. The knockdown of MDM2-5 by RNAi caused a higher level of NADH2 and ATPsyn and a higher freezing tolerance of hydrated seeds. This indicated that MDM2 played negative roles in regulating ATP synthesis and freezing tolerance of hydrated seeds.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"55 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Korean water deer (Hydropotes inermis argyropus) feces on seed germination and early seedling growth: insights into their contribution to seed dispersal

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-01-24 DOI: 10.1017/s096025852400028x

Seung-Kyung Lee, Eun Ju Lee

{"title":"Effects of Korean water deer (Hydropotes inermis argyropus) feces on seed germination and early seedling growth: insights into their contribution to seed dispersal","authors":"Seung-Kyung Lee, Eun Ju Lee","doi":"10.1017/s096025852400028x","DOIUrl":"https://doi.org/10.1017/s096025852400028x","url":null,"abstract":"Endozoochory, the dispersal of seeds through the animal gut passage, plays a significant role in vegetation dynamics. The success of endozoochorous seed dispersal depends on each stage of the process: ingestion by animals, gut passage, and post-dispersal events after defecation. After the deposition of seeds through feces, the effects of feces on the initial stages of seedling establishment, including seed germination and seedling growth, can significantly impact overall survival. The pattern of fecal effects on plant species depends on the animal species. In this study, we investigated the effects of feces presence on seed germination and early seedling growth using feces of the Korean water deer (Hydropotes inermis argyropus). We conducted a germination experiment on 12 plant species belonging to 10 plant families, which are known to germinate in the feces of Korean water deer. The study compared the seed germination rate and seedling length after germination between seeds sown with and without feces of the Korean water deer. In general, we found that the presence of deer feces per se had no significant effects on seed germination and early growth stages. However, additional research on post-dispersal events such as long-term growth, fecal type, and germination conditions is needed to fully understand the costs and benefits of endozoochory.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"49 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an extraction method for the identification of peptides in the spermosphere of common bean (Phaseolus vulgaris L.)

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-01-24 DOI: 10.1017/s0960258525000030

Chandrodhay Saccaram, Benoit Bernay, Céline Brosse, Boris Collet, Shuang Peng, Delphine Sourdeval, Tracy François, Massimiliano Corso, Loïc Rajjou

{"title":"Development of an extraction method for the identification of peptides in the spermosphere of common bean (Phaseolus vulgaris L.)","authors":"Chandrodhay Saccaram, Benoit Bernay, Céline Brosse, Boris Collet, Shuang Peng, Delphine Sourdeval, Tracy François, Massimiliano Corso, Loïc Rajjou","doi":"10.1017/s0960258525000030","DOIUrl":"https://doi.org/10.1017/s0960258525000030","url":null,"abstract":"Seeds are crucial for plant reproduction, dispersal and agriculture. Seed quality and vigour greatly impact crop production by enabling rapid and uniform germination under various environmental conditions. This leads to healthy seedlings that can withstand both biotic and abiotic stresses, which are particularly important in the context of the accentuation of global climate change. Upon imbibition during germination sensu stricto, seeds release exudates, complex mixtures of organic and inorganic molecules, into the microenvironment surrounding them, known as the spermosphere. These exudates play a pivotal role in seedling development and overall plant fitness by influencing microbial selection, growth and interactions in the spermosphere, ultimately shaping the plant's microbiome. Proteins such as enzymes with protection properties have previously been demonstrated to be released by the seeds in their exudates. However, limited information is available pertaining to peptides in seed exudates. Here, we developed an experimental protocol to extract and identify peptides in the spermosphere of germinating common bean seeds. We showed that our methodology was successful in identifying a broad spectrum of peptides and that extraction solvent choice impacts peptide identification both qualitatively and quantitatively. We also show the possibility of using online prediction tools to predict the properties of identified peptides based on their amino acid sequence. We propose that this approach may be used to identify potential molecules that could be used as candidates for developing strategies to enhance seed quality and improve crop productivity.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"58 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of the mucilage reduces intact seed passage through the digestive system of birds 去除粘液会减少完整的种子通过鸟类的消化系统

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-01-23 DOI: 10.1017/s0960258524000187

A. Kreitschitz, E. Haase, S.N. Gorb

{"title":"Removal of the mucilage reduces intact seed passage through the digestive system of birds","authors":"A. Kreitschitz, E. Haase, S.N. Gorb","doi":"10.1017/s0960258524000187","DOIUrl":"https://doi.org/10.1017/s0960258524000187","url":null,"abstract":"Many plants developed unique adaptations such as protective seed coatings or eatable fruits/seeds that aid in seed dispersal by animals. Birds are among the animals that perform endozoochoric diaspore dispersal. In this study, we used mechanically demucilaginated seeds and seeds covered with artificial mucilage with the aim of determining the protective potential of the mucilage envelope against seed digestion during endozoochory by birds. We removed the mucilage from the seeds of Plantago lanceolata, Plantago ovata, Plantago psyllium, and Linum usitatissimum and fed the seeds to pigeons. Using naturally non-mucilaginous seeds of Amaranthus albus, Brassica napus and Nigella sativa, we tested the survival of diaspores covered with artificial mucilage (P. ovata husk) in the digestive systems of pigeons. Among the demucilaginated diaspores (2700 per taxon), no seeds of L. usitatissimum, 1 seed of P. lanceolata, 1 seed of P. ovata and 7 seeds of P. psyllium passed through the bird gut intact. This indicated that after removing the mucilage envelope, few diaspores can pass through the digestive system. Among diaspores with artificial mucilage, 7 seeds (from 8100 total) passed through the bird gut intact. This indicated that the artificial mucilage did not support the survival of the seeds as they passed through the pigeon digestive system. Furthermore, germination experiments revealed a decrease in the germination rate of demucilaginated seeds. They moulded rapidly, and only a few of the seeds germinated. The results of this study demonstrate the significant role of the mucilage in preventing digestion and supporting diaspore germination during endozoochory.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"120 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seed longevity: analysing post-storage germination data in R to fit the viability equation 种子寿命：在R中分析储藏后萌发数据以拟合活力方程

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-01-13 DOI: 10.1017/s0960258524000291

Dustin Wolkis, Angelino Carta, Shabnam Rezaei, Fiona R. Hay

{"title":"Seed longevity: analysing post-storage germination data in R to fit the viability equation","authors":"Dustin Wolkis, Angelino Carta, Shabnam Rezaei, Fiona R. Hay","doi":"10.1017/s0960258524000291","DOIUrl":"https://doi.org/10.1017/s0960258524000291","url":null,"abstract":"For many decades, seed germination data have been modelled by probit analysis. In particular, it is the basis of the seed viability equation used, in the first instance, to describe the decline in germination of seeds in storage, but then also the rate of the decline, depending on seed moisture content and the temperature of storage. The underlying assumption of a probit model is that the response follows a normal distribution, in this case, loss of the ability to germinate over time. Probit analysis also takes into account the binomial error associated with germination data. Many statistical packages have probit analysis as an option within the generalized linear modelling framework; here, we present code for applying probit analysis in the free software, R. Codes are provided for fitting a single survival curve, for a single seed lot stored in a constant storage environment; for fitting multiple survival curves and evaluating the effect of constraining parameters for the different seed lots; and lastly, to model the moisture relations of seed longevity. The code bases provided could also be used in pollen and fern/bryophyte spore longevity modelling.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"3 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre- and post-harvest temperatures influence the germination response to supra-optimal temperature in contrasting tomato (Solanum lycopersicum) MAGIC genotypes 收获前和收获后温度影响MAGIC基因型番茄种子萌发对超适温度的响应

IF 2.1 3区生物学

Seed Science Research Pub Date : 2025-01-13 DOI: 10.1017/s0960258524000217

Abdulsatar Mohammad, Benoît Ly Vu, Joseph Ly Vu, Elise Bizouerne, Julia Buitink, Olivier Leprince

{"title":"Pre- and post-harvest temperatures influence the germination response to supra-optimal temperature in contrasting tomato (Solanum lycopersicum) MAGIC genotypes","authors":"Abdulsatar Mohammad, Benoît Ly Vu, Joseph Ly Vu, Elise Bizouerne, Julia Buitink, Olivier Leprince","doi":"10.1017/s0960258524000217","DOIUrl":"https://doi.org/10.1017/s0960258524000217","url":null,"abstract":"Seeds rely on temperature to adjust their germination timing by modulating primary and secondary dormancy. The knowledge regarding an intraspecific variation in the germination responses to supra-optimal temperatures during imbibition within the Solanum lycopersicon species and its relation with pre- and post-harvest environments is limited. Here, we studied the impact of imbibition at 35°C in 17 genotypes selected from a multiparent advanced generation intercross (MAGIC) population. We discovered a high genetic variability in the germination responses to heat, leading to thermotolerance, thermoinhibition or thermodormancy with different depths. While thermodormancy appeared more profound than primary dormancy, there was no correlation between the deepness of primary and thermodormancy. Post-harvest treatments influenced considerably germination at supra-optimal temperatures. Dry storage beyond the apparent loss of primary dormancy led to an increased proportion of thermotolerant or thermoinhibited seeds at the expense of thermodormancy in a genotype-dependent manner, thereby revealing cryptic genetic variation. Prolonged cold imbibition also led to increased thermodormancy in genotypes that produced thermotolerant and thermoinhibited seeds. The thermal history before and after flowering influenced primary dormancy and the germination response to heat during imbibition in a genotype-dependent manner, with high temperatures leading to increased thermotolerance or thermoinhibition at the expense of thermodormancy, suggesting transgenerational plasticity despite the domestication of the species. The high potential of the MAGIC population for quantitative trait loci mapping and causal polymorphism identification will be helpful in deciphering the regulatory mechanisms that lead to the plasticity of thermoinhibition or thermodormancy, as well as their connection to the parental environment.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"20 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orchid embryo-to-seed (E:S) ratio as an indicator of germination behaviour and its ecological implications 兰花胚种比（E:S）作为萌发行为的指标及其生态学意义

IF 2.1 3区生物学

Seed Science Research Pub Date : 2024-12-26 DOI: 10.1017/s0960258524000242

Spyridon Oikonomidis, Costas A. Thanos

{"title":"Orchid embryo-to-seed (E:S) ratio as an indicator of germination behaviour and its ecological implications","authors":"Spyridon Oikonomidis, Costas A. Thanos","doi":"10.1017/s0960258524000242","DOIUrl":"https://doi.org/10.1017/s0960258524000242","url":null,"abstract":"Although the seeds of various Orchidaceae members can be readily germinated on sterile nutrient media, numerous species exhibit complete asymbiotic germination failure. Also, while seed morphology in orchids has been previously linked to dispersal, associations with germinability have not been widely explored. We compiled seed morphometric and germination data for 203 orchid species globally, drawing from international literature and our lab's unpublished findings. Based on (a) final germination percentage and (b) pre-treatment duration, two distinct groups of asymbiotic germination behaviour emerged – termed compliant and defiant, respectively. Additionally, a statistically significant relationship between germinability and the embryo-to-seed (E:S) length ratio was observed. E:S values tended to be lower in species with defiant germination and higher in those with compliant germination. We further correlated seed morphometric and germination data with phylogenetically and ecologically significant parameters (subfamily, growth form, mycoheterotrophy level, climatic zone and habitat shadiness). Notably, lower E:S values and defiant germination predominate in orchids of shaded habitats, while compliant germination is more prevalent in open habitats. Lastly, considering the mycoheterotrophy continuum, we propose that the reduction in the E:S ratio is linked to the adaptation of various orchid lineages to shaded habitats through both an increased dispersal ability and their greater reliance on fungal symbiosis.","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"32 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0