Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies最新文献

{"title":"Morphogenesis of immune formations of ducks' intestine","authors":"T. Mazurkevych, S. Usenko","doi":"10.32718/nvlvet11215","DOIUrl":"https://doi.org/10.32718/nvlvet11215","url":null,"abstract":"The morphogenesis of immune formations of duck intestines was studied. Material for histological studies was taken from Blagovarsky cross broiler ducks aged from one to 420 days. The obtained sections were stained with Karatsi's hematoxylin and eosin – to establish the features of the microscopic structure of intestinal immune formations, Weigert's hematoxylin and picrofuchsin according to Van Gieson and aniline blue in combination with acid fuchsin and Orange G according to Mallory – to identify collagen fibers, resorcin-fuchsin according to Weigert – for differentiation of elastic fibers. To determine reticular fibers, sections were impregnated with 1–2 % argentum nitrate solution according to the modified Kelemen method. Electron microscopy studies were carried out to study the ultrastructure of the immune formations of the intestines of 180-day-old ducks. The studies have established that the intestinal wall in the places where immune formations of the intestines in ducks are localized is formed by tunica mucosa, tunica muscularis and tunica serosa, the area of which in all immune formations changes unevenly and asynchronously with the birds’ age. The tunica mucosa occupies the largest area, which changes unevenly and asynchronously, reaching maximum values (from 71.66 ± 0.25 to 81.28 ± 0.19 %) during the first 25 days of the ducks' life. The tunica muscularis occupies a smaller area than the tunica mucosa. It decreases unevenly in the first 25 days of a bird’s life, and in older birds it increases and reaches maximum values (35.68 ± 0.49–46.67 ± 1.39 %) in 330- and 420-day-old birds. The tunica serosa occupies the smallest area. In day-old birds, this indicator is the highest (from 2.47 ± 0.07 to 6.20 ± 0.02 %) and decreases with age by 1.24–6.52 times. Lymphoid tissue, which determines the functions of immune formations, is located in their tunica mucosa and tunica muscularis. It is detected in the tunica mucosa of all immune formations and the tunica muscularis of caecal diverticula from the bird’s one-day age, in the tunica muscularis of other immune structures – from the age of 10–20 days. The total area of lymphoid tissue increases from one day (27.03 ± 0.88–31.72 ± 0.04 %) to 150 days of age in ducks in Peyer's patches of the duodenum and jejunum and cecal diverticula (59.86 %, 59.58 ± 1.28, 65.12 ± 0.50 %), up to 210 days of age in Peyer’s patches of the ileum (71.64 ± 1.03 %), up to 330 days of age in Peyer’s patches of the cecum (37.84 ± 5.12 %), up to 420 days of age in Meckel’s diverticulum (55.24 ± 0.37 %). In older birds, the area of lymphoid tissue decreases, which indicates the beginning of its involution and, accordingly, the involution of immune formations.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"136 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Globigen Jump Start feed additive in feeding lactating sows and piglets","authors":"T. Y. Prudyus, Y. I. Kyryliv","doi":"10.32718/nvlvet11217","DOIUrl":"https://doi.org/10.32718/nvlvet11217","url":null,"abstract":"Modern methods of pig breeding increasingly use various immunotropic, probiotic agents and feed additives that affect the intestinal microbiota when used in feeding farrowing and lactating sows. The research was conducted in the conditions of a pig farm in the Kyiv region. The number of sows at the pig complex is 500 heads, PIC genetics. Clinically healthy sows with 2–3 farrowing and without rest with similar performance were selected for the study. Two groups of sows were formed by analogs, which were kept in one farrowing ward 5 days before farrowing, with 8 heads in each of the control and experimental groups. For the experiment on sows and piglets in the early rearing period, the Globigen Jump Start feed additive was used. We studied the effect of feeding Globigen Jump Start feed additive, which contains specific lgY immunoglobulins in rations for gilt sows and piglets in the early growing period in the amount of 1 kg and 2 kg per ton of compound feed, respectively. As a result of research, it was established that feeding the drug mentioned above to sows contributed to better preservation of the live weight of lactating sows by 20% compared to sows of the control group. Under the influence of the addition of a feed additive, the quality of sow milk improved. In particular, there was a tendency to increase certain amino acids (cystine, valine, isoleucine, arginine, lysine, and glutamic acid) and some macro- and microelements (magnesium, zinc, sodium, phosphorus, and slightly calcium). Adding the Globigen Jump Start feed additive to piglets' diet increased average daily gains by 18.52%, a decrease in diarrhea by 45.83%, and better feed conversion.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"36 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Definition of Acute Toxicity and Cumulative Properties of the Drug “Lipointersil”","authors":"Y. Lavryshyn, B. Gutyj, B. Verveha, O. Kutsan, V. Hunchak, I. Khariv, V. Kushnir, R. O. Vasiv, K. Leskiv, Z. Guta","doi":"10.32718/nvlvet11214","DOIUrl":"https://doi.org/10.32718/nvlvet11214","url":null,"abstract":"The research aimed to determine the acute toxicity and cumulative properties of the liposomal drug “Lipointersil” based on interferon and spotted milk thistle. The acute toxicity of the “Lipointersil” drug was assessed in white rats, aged 2–3 months, with a body weight of 170–180 g through intragastric and intramuscular administration. After administration of the drug, the dose and the number of animals that died were recorded, and the median lethal dose (DL50) of the investigated drug was calculated using the method of H. Kerber. The cumulative properties were determined in white rats aged 2–3 months, weighing 170–185 g, using the subchronic toxicity test method by K. S. Lim et al., modified by K. K. Sydorov. Based on the conducted research, it was established that the “Lipointersil”drug, according to the State Standard of Ukraine 85.2-37-736:2011, belongs to class IV toxicity (slightly toxic substances). The LD50 for intragastric and intramuscular administration in white rats is 5166.66 and 5833.33 mg/kg of body weight, respectively. In determining of the cumulative properties of the “ Lipointersil ” drug, no deaths of experimental animals were observed during the study. Moreover, the animals remained active, fed well, and had dense, shiny fur. The coefficient of accumulation of the “Lipointersil” drug was more than 8.31 units, indicating its weakly pronounced cumulative properties. Prolonged daily administration for 24 days of “Lipointersil” had no significant impact on the functional state of the liver and kidneys. Under long-term (24 days) daily administration in increasing doses, the drug “Lipointersil” caused slight destruction of hepatocyte membranes, as indicated by the increased activity of alanine and aspartate aminotransferases and alkaline phosphatase.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"25 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of antibacterial activity of tinctures from leaves of Pyrus communis of Noyabrskaya variety, Arctostaphylos adans, rhizomes of Potentilla erecta and Poltava Bischofite","authors":"A. M. Khyl, S. B. Peredera","doi":"10.32718/nvlvet11221","DOIUrl":"https://doi.org/10.32718/nvlvet11221","url":null,"abstract":"The bactericidal effect of medicinal plants (Pyrus communis of Lystopadova variety, Arctostaphylos Adans, rhizomes of Potentills Erecta and Bischofite) on Staphylococcus aureus strain 209 and Escherichia coli strain 1257 was studied. According to the literature, it was found that the chemical composition of the studied plants contains flavonoids, phenolglycosides, tannins, triterpenoids, amino acids, essential oil, hydroxycinnamic acids, saponins, catechin, sanguinarin, which have antimicrobial effects. The objects of the study were 40 % tinctures of the leaves of Pyrus communis Noyabrskaya, Arctostaphylos Adans, and rhizomes of Potentilla erecta. The antimicrobial activity of plant-based herbal remedies was studied using the generally accepted method of “wells”. It was found that the tinctures in combination: 1) Arctostaphylos Adans with the addition of Potentills Erecta, Pyrus communis, Bischofite solution and 2) Arctostaphylos Adans + of Potentills Erecta + Bischofite have a more pronounced antimicrobial effect compared to other studied herbal remedies. The least pronounced effect of the tincture of pear leaf extract + bischofite was on Staphylococcus aureus strain 209 than on Escherichia coli strain 1257. The presence of the antimicrobial effect of tinctures from raw materials of Pyrus communis of the Noyabrskaya variety, Arctostaphylos Adans, galangal rhizome, and Bischofite indicates the expediency of further study of this composition. The presence of active substances with different pharmacological activity in plants contributes to the effective prevention of diseases of infectious etiology without side effects on the animal and human body. The raw materials of medicinal plants are less likely to contribute to various complications and, unlike traditional antibacterial drugs, in addition to antimicrobial action, have a mild positive effect on the macroorganism.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"138 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of milk thistle and silimevit on the functional state and protein synthesizing-function of the liver of laying hens under conditions of cadmium load","authors":"A. Y. Ostapyuk, B. Gutyj, L. Horalskyi, R. Mylostyvyi, I. Sokulskyi, A. A. Butsyak, S. S. Popadiuk, V. V. Senechyn, K. Leskiv","doi":"10.32718/nvlvet11224","DOIUrl":"https://doi.org/10.32718/nvlvet11224","url":null,"abstract":"The purpose of the work was to study the effect of Cadmium on liver function disorders and to develop practical recommendations for using silimevit and milk thistle to reduce the toxic effect of Cadmium on chickens. To achieve the set goal in experiments on chickens under cadmium load, it was necessary to solve the following tasks: to study the effect of Cadmium on the protein synthesis function of the liver of laying hens; to study the effect of Cadmium on the functional state of the liver of laying hens; to investigate the effect of silimevit and the fruits of milk thistle on the functional state of the liver of laying hens under cadmium load; to investigate the effect of silimevit and milk thistle fruits on the protein synthesis function of the liver of laying hens under cadmium load and to justify the use of silimevit and milk thistle to prevent cadmium toxicosis in chickens. Drinking cadmium sulfate to laying hens violates the liver's functional state and protein-synthesizing function. A decrease in total protein and albumin content in their blood was established (Р < 0.001). An increase in alanine and aspartate aminotransferase activity was detected (Р < 0.001). Silimevit and milk thistle positively affect the functional state of the liver of laying hens under cadmium load, as evidenced by a decrease in the activity of aminotransferases in their blood serum. Silimevit and spotted thistle, under the cadmium load of laying hens, positively affect the liver's protein-synthesizing function, evidenced by an increase in total protein and albumin fraction. Feeding laying hens under cadmium load with slime feed contributed to a better-normalizing effect on the activity of aminotransferases and protein-synthesizing function of the chickens' liver than milk thistle.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"10 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139187396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The state of the body's immune system of beef cows with signs of endotoxicosis","authors":"I. R. Lozynskyi, B. Gutyj, R. M. Ivashkiv, M. M. Ilchyshyn, T. Martyshuk, V. Todoriuk, O. O. Dashkovskyi, N. Magrelo, Н. V. Sus, V. V. Voroniak, U. Vus","doi":"10.32718/nvlvet11213","DOIUrl":"https://doi.org/10.32718/nvlvet11213","url":null,"abstract":"During the development of endotoxicosis of various etiologies, secondary immunodeficiency occurs in animals, which progresses due to exposure to toxic metabolites. The work aimed to study the influence of endotoxicosis on the dynamics of indicators of the immune system of beef cows. The research was conducted on the Ukrainian black and spotted dairy breed beef cows. In cows with signs of endotoxicosis, clinical signs such as swelling of the external genital organs and mammary gland, anemia of the mucous membranes, depressed state, impaired appetite, and functional disorders of the antrum and intestines were characteristic. The main immunological tests characterizing the state of the immune system of beef cows are indicators of humoral immunity (bactericidal and lysozyme activity of blood serum), cellular immunity (T- and B-lymphocytes), indicators of non-specific resistance of the organism (phagocytic activity and phagocytic index). In beef cows with clinical manifestations of endotoxicosis, inhibition of cellular, humoral, and non-specific links of the immune system was established, which led to the development of the so-called secondary immunodeficiency. This is evidenced by a decrease in the bactericidal and lysozyme activity of blood serum, a decrease in the number of T- and B-lymphocytes, and a decrease in the phagocytic index and phagocytic activity of the blood of sick cows. In particular, it was established that the lowest indicator of bactericidal and lysozyme activity was observed in the 9th month of pregnancy in the experimental group of cows, where it decreased by 15.9 and 4.91 %, respectively, compared to the control. In the above study periods, a decrease in the phagocytic activity of neutrophils by 5.04 % and the phagocytic index by 33.3 % was established compared to the indicators of the control group. During the analysis of cellular immunity, similar changes were found in determining the number of T- and B-lymphocytes; they were lower by 12.5 % and 4.19 %, respectively. Changes in the indicators of the immune system are significant and objective markers of the state of the cow's body during the development of endotoxicosis, regardless of its causes.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"44 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspectives of the use of macro- and micro elements in poultry feeding","authors":"R. O. Borkovskyi, A. Berezovsky","doi":"10.32718/nvlvet11211","DOIUrl":"https://doi.org/10.32718/nvlvet11211","url":null,"abstract":"Poultry breeding is one of the fastest growing segments of the agricultural sector in many countries of the world, including Ukraine. Currently, Ukraine ranks 8th in terms of production of eggs and broilers in the world. A sufficient amount of minerals and vitamins in the diet is the key to good production of poultry products. Feeding feed deficient in vitamins and minerals can cause numerous health problems in chickens, including death in some cases. The poultry farmer must monitor the health of the chickens every day. Therefore, the development of the practice of feeding a balanced diet with the necessary amount of minerals to prevent premature disease of poultry caused by nutrient deficiency is relevant today. In poultry farming, mineral substances are used as one of the ways to increase metabolism in birds. Mineral substances are part of the molecules of complex organic structures that participate in metabolic processes, including digestion, thereby improving the assimilation and use of feed nutrients. The lack of microelements in feed causes diseases of the joints of the limbs of poultry, a decrease in its productivity, and a deterioration in the quality of eggshells. Zinc, copper, manganese, iron, iodine, selenium, cobalt are important trace elements in the feed of broiler chickens. Therefore, the effectiveness of the use of trace elements is an actual topic of modern poultry feeding. Mineral substances are useful in poultry farming, as they provide prevention of various diseases. Organic minerals in small amounts can be added to the diet of birds, because minerals are better absorbed by the bird than mineral salts. Demand for alternative natural feed additives with mineral content is important, especially as an alternative to antibiotics. They can have a preventive effect against various diseases, participate in numerous physical and biochemical mechanisms and basic processes of growth and development. However, further large-scale research is needed for the further use of macro- and micronutrients in commercial poultry farming.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"33 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of bacterial contamination of the mammary gland secretion of lactating cows with subclinical mastitis","authors":"A. M. Pasternak, V. Koshevoy, S. Naumenko, M. L. Radzykhovskyi, P. Skliarov","doi":"10.32718/nvlvet11218","DOIUrl":"https://doi.org/10.32718/nvlvet11218","url":null,"abstract":"Subclinical mastitis (SCM) in lactating cows is a common disease characterized by a change in the composition of mammary gland secretion, especially due to an increase in number of somatic cells in it over a long period of time. Etiologically, this disease arises as a result of the pathogenic effect of mixed infections caused mainly by bacteria of Staphylococcus and Streptococcus genera. It should be noted that due to high prevalence, reduction of milk yield and changes in the physical and chemical properties of milk of cows, SCM is a more important problem than clinical forms of mastitis. Thus, the aim of the work was to determine the main causative agents of SCM in lactating cows and their resistance to antibiotics in order to justify the feasibility of using alternative means in the schemes of therapy and prevention of this disease. During the bacteriological examination of mammary gland secretion, streptococci were differentiated by morphological and cultural-biochemical properties, and the pathogenicity of staphylococci was determined by the ability to coagulate rabbit blood plasma in the conditions of the testing laboratory of Smartbiolab LLC (Kharkov) in 2019–2021. Subclinical mastitis in lactating cows was determined using a sample with 5 % dimastine and a thermographic study. Before taking milk samples, the udders were wiped with 70 % alcohol, and the milk was expressed in sterile tubes. The samples were transported in plastic boxes with cooling elements (at a temperature of 8–10 °С) and were examined no later than 4 hours after the moment of selection. The results of research in selected samples Streptococcus spp., which has β-hemolytic properties, was found in 42.9 % of samples, E. coli with hemolytic properties in 28.6 %, and Staphylococcus haemolyticus and Staphylococcus aureus in 14.2 % each. The detected pathogens were characterized by the following indicators of antibiotic resistance: E. coli isolates were insensitive to 8 antibiotics (ampicillin, penicillin, streptomycin, neomycin, spectinomycin, spiramycin, lincomycin, chloramphenicol), and Streptococcus spp. (penicillin, amoxicillin, amoxicillin + clavulonic acid, streptomycin, norfloxacin, gatifloxacin, lincomycin) and Staphylococcus aureus (ampicillin, penicillin, amoxicillin, streptomycin, oxytetracycline, ceftiofur and lincomycin) up to 7, respectively.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"94 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute toxicity of zinc carbonate nanocrystals on white mice model","authors":"V. Koshevoy, S. Naumenko, O. Orobchenko, I. Bespalova","doi":"10.32718/nvlvet11220","DOIUrl":"https://doi.org/10.32718/nvlvet11220","url":null,"abstract":"Zinc-based nanoparticles (NPs) are successfully used to meet the mineral needs of animals and poultry, capable of increasing the growth rate and the efficiency of their use of feed. Nanoparticles of zinc oxide are widely used, which are superior to conventional sources of zinc in terms of pharmacological activity and bioavailability, have a positive effect on productivity and antioxidant protection, and increase reproductive potential. However, a detailed investigation of their toxicological parameters showed the risks of their introduction into the body, as a result of which a large number of zinc oxide NPs were synthesized by the methods of “green chemistry”, the main drawback of which is a complex methodology and a high level of costs. We investigated the acute toxicity of a colloidal solution of zinc carbonate nanocrystals in a model of white mice. The experiment was conducted on 42 female non-linear white mice, 3–4 months old, with a live weight of 21–23 g, kept under optimal vivarium conditions. According to the principle of analogues, 6 experimental groups were formed: mice were injected with a colloidal solution of zinc carbonate nanoparticles in doses (by absolute weight of the drug) of 15,000.0; 20000.0; 25000.0; 30000; 35000.0 and 40000.0 mg/kg of body weight based on the absolute weight of the drug, which according to the active substance (zinc carbonate nanoparticles) was (37.5; 50.0; 62.5; 75.0; 87.5 and 100, 0 mg/kg of body weight), orally using an esophageal-gastric tube. Mice of the control group were injected with distilled water in a volume of 0.2 cm3 according to the same protocol. The clinical condition of the experimental animals was observed for 14 days, noting the appearance and development of clinical signs of poisoning, the time of death or recovery to the physiological norm. After the death (diagnostic slaughter) of the animals, a pathological autopsy was performed. Clinical observations showed that a single intragastric administration of the drug to mice of experimental groups 1-6 did not cause acute poisoning. The deaths of mice in all experimental groups were not observed during the 14-day observation period, as a result of which the LD50 indicator could not be calculated. At the same time, the maximum possible injected dose of the colloidal solution of zinc carbonate nanoparticles (by absolute weight) was 40,000.0 mg/kg of body weight (100.0 mg/kg by active substance), which allows the drug to be assigned to the VI class of toxicity – substances are relatively harmless (LD50 > 15000.0 mg/kg of body weight), and according to the degree of danger up to IV class – low-hazardous substances (LD50 > 5000.0 mg/kg of body weight).","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"39 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the therapeutic effectiveness of the veterinary medicinal product “Surfadev” in complex therapy for stimulating sexual desire in cows","authors":"O. A. Katsaraba, R. M. Sachuk, Y. Kostyshyn, Y. Stravskyy, Lidia Kostyshyn","doi":"10.32718/nvlvet11223","DOIUrl":"https://doi.org/10.32718/nvlvet11223","url":null,"abstract":"Clinical studies were conducted in the treatment of hypofunction of the ovaries to increase the fertility of cows and heifers with the newly created drug “Surfadev” (solution for injections) in complex therapy. VLZ “Surfadev” (solution for injections). The drug stimulates the secretion of gonadotropins by the pituitary gland in a maximum of 2–3 hours after administration. The increased content of gonadotropins in the blood persists for 4–5 hours after administration. Unlike natural luliberin, the biological activity of the drug is 50 times greater, which allows the use of this drug in microdoses and short courses. The drug decomposes under the influence of enzymes more slowly than natural luliberin, which provides a stronger biological effect on the pituitary gland. “Devivit Complex” is a complex multivitamin preparation (Vitamins A, D3, E, B1, B2, B3, B5, B6, B12), which is used to correct and normalize metabolic processes in animals. Vitamins included in the preparation take part in biochemical processes in animal organisms (in the form of enzyme vitamins, hormone vitamins, antioxidants). When ovarian function is stimulated or corrected, 90.0 % of the treated animals show the arousal stage of the sexual cycle within 3–15 days, and their fertilization rate from the first insemination is 70.0 % on average. The high efficiency of using “Surfadev” in combination with “Devivit Complex” for ovarian hypofunction has been established. 83.3–95.6 % of the treated cows showed sexual desire, and the fertility from the first insemination reached 70.0 %. Further studies will be the next stage of pre-registration trials aimed at studying the effectiveness of the veterinary drug “Surfadev” (solution for injections) in complex therapy in the treatment of ovarian hypofunction and follicular cysts, which is mandatory material of the fourth part “Preclinical and clinical documentation” of the dossier for this medicine.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139187458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}