药物 "Lipointersil "的急性毒性和累积特性的定义

Y. Lavryshyn, B. Gutyj, B. Verveha, O. Kutsan, V. Hunchak, I. Khariv, V. Kushnir, R. O. Vasiv, K. Leskiv, Z. Guta
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摘要

研究旨在确定基于干扰素和斑点奶蓟草的脂质体药物 "Lipointersil "的急性毒性和累积特性。通过胃内和肌肉注射,对体重为 170-180 克、年龄为 2-3 个月的白鼠进行了 "Lipointersil "药物急性毒性评估。给药后,记录了剂量和死亡动物的数量,并采用 H. Kerber 的方法计算了所研究药物的中位致死剂量(DL50)。K. S. Lim 等人采用经 K. K. Sydorov 修改的亚慢性毒性试验方法,测定了体重 170-185 克、2-3 个月大的白鼠的累积特性。研究结果表明,根据乌克兰国家标准 85.2-37-736:2011,"Lipointersil "药物属于第四类毒性(微毒物质)。白鼠胃内和肌肉注射的半数致死剂量分别为 5166.66 毫克/千克体重和 5833.33 毫克/千克体重。在确定 "Lipointersil "药物的累积特性时,研究期间没有发现实验动物死亡。此外,实验动物依然活跃,食欲良好,皮毛浓密有光泽。Lipointersil "药物的蓄积系数超过 8.31 单位,表明其蓄积性较弱。连续 24 天每天长期服用 "Lipointersil "对肝脏和肾脏的功能状态没有明显影响。在长期(24 天)每天增加剂量给药的情况下,"Lipointersil "药物会对肝细胞膜造成轻微破坏,表现为丙氨酸和天门冬氨酸转氨酶以及碱性磷酸酶的活性增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Definition of Acute Toxicity and Cumulative Properties of the Drug “Lipointersil”
The research aimed to determine the acute toxicity and cumulative properties of the liposomal drug “Lipointersil” based on interferon and spotted milk thistle. The acute toxicity of the “Lipointersil” drug was assessed in white rats, aged 2–3 months, with a body weight of 170–180 g through intragastric and intramuscular administration. After administration of the drug, the dose and the number of animals that died were recorded, and the median lethal dose (DL50) of the investigated drug was calculated using the method of H. Kerber. The cumulative properties were determined in white rats aged 2–3 months, weighing 170–185 g, using the subchronic toxicity test method by K. S. Lim et al., modified by K. K. Sydorov. Based on the conducted research, it was established that the “Lipointersil”drug, according to the State Standard of Ukraine 85.2-37-736:2011, belongs to class IV toxicity (slightly toxic substances). The LD50 for intragastric and intramuscular administration in white rats is 5166.66 and 5833.33 mg/kg of body weight, respectively. In determining of the cumulative properties of the “ Lipointersil ” drug, no deaths of experimental animals were observed during the study. Moreover, the animals remained active, fed well, and had dense, shiny fur. The coefficient of accumulation of the “Lipointersil” drug was more than 8.31 units, indicating its weakly pronounced cumulative properties. Prolonged daily administration for 24 days of “Lipointersil” had no significant impact on the functional state of the liver and kidneys. Under long-term (24 days) daily administration in increasing doses, the drug “Lipointersil” caused slight destruction of hepatocyte membranes, as indicated by the increased activity of alanine and aspartate aminotransferases and alkaline phosphatase.
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