M. Alves, Fagner D'ambroso Fernandes, Patrícia Bräunig, H. F. D. Santos, L. Sangioni, F. Vogel
{"title":"Detecção molecular de Sarcocystis spp. no músculo peitoral de aves de cativeiro da região sul do Brasil","authors":"M. Alves, Fagner D'ambroso Fernandes, Patrícia Bräunig, H. F. D. Santos, L. Sangioni, F. Vogel","doi":"10.5433/1679-0359.2023v44n1p135","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p135","url":null,"abstract":"Estudos sobre doenças de aves silvestres são essenciais no contexto da saúde pública, pois esses animais atuam como sentinelas, permitindo obter informações sobre uma determinada área geográfica. Além disso, as aves são fontes de proteína alimentar para os animais e, portanto, desempenham um papel importante no ciclo de vida do Sarcocystis. Este estudo teve como objetivo identificar Sarcocystis spp. nos músculos do peito de aves de cativeiro naturalmente infectadas. Os músculos do peito de 89 aves foram coletados, e o DNA amplificado pela PCR do gene RNA ribossômico 18S para detecção de Sarcocystis spp. Os produtos da PCR foram sequenciados e 5,61% (5/89) amostras apresentaram 100% de similaridade com o Sarcocystis spp. (um Cyanoliseus patagonus, um Psittacula krameri, dois Pyrrhura frontalis e um Ramphastos dicolorus). O grande número de espécies naturalmente infectadas analisadas por métodos moleculares permitiu a detecção de Sarcocystis spp. em diferentes espécies de aves, corroborando a epidemiologia de Sarcocystis spp. Até onde sabemos, este é o primeiro relato especificando a presença de Sarcocystis spp. no músculo do peito de espécies de aves.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46010131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic correlation between feed efficiency and carcass traits in Nellore cattle in Brazil","authors":"M. Gomes, R. Lôbo, C. Faria","doi":"10.5433/1679-0359.2023v44n1p97","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p97","url":null,"abstract":"The aim was to estimate the genetic correlations between residual feed intake (RFI) and dry matter intake (DMI) with carcass finish (CF), rib eye area (REA), and marbling (MAR) of Nellore cattle. Data from 7,808 animals were considered. In addition, data from 2,261 females included in the complete database were also considered. Estimates of variance and covariance components, as well as heritabilities and genetic correlations were obtained by means of two-character analysis under animal model. Heritability estimates were found to be moderate for the RFI (0.22) and DMI (0.29) traits. It was observed that genetic correlation was close to zero for all traits, except between RFI and REA (-0.11). However, considering the female population, there was an increase in the estimated genetic correlation between RFI and DMI, although still a favorable genetic association of low magnitude (-0.30). There was also an increase in the genetic association of REA with RFI (-0.21). It can be concluded that the direct selection for RFI and DMI will not influence the CF, MAR, or REA of Nellore cattle. However, this selection may generate some favorable responses in MAR and REA in Nellore females.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44242523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dailis Delazeri, Kate Aparecida Buzi, M. Neumann, Patricia Santos Rossi, Tiefanny Zart, Gabriela Garbossa, Loisa Padilha Hintz, Heloisa Godoi Bertagnon
{"title":"Suplementação com Saccharomyces cerevisae autolisada reduz contaminação microbiológica da carcaça de novilhos terminados em confinamento","authors":"Dailis Delazeri, Kate Aparecida Buzi, M. Neumann, Patricia Santos Rossi, Tiefanny Zart, Gabriela Garbossa, Loisa Padilha Hintz, Heloisa Godoi Bertagnon","doi":"10.5433/1679-0359.2023v44n1p123","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p123","url":null,"abstract":"Este trabalho objetivou verificar se o prebiótico a base de parede celular de Saccharomyces cerevisiae reduz coliformes totais, Escherichia coli e Salmonella spp da pele, fezes e da carcaça de bovinos terminados em confinamento. Para tanto, 18 novilhos, ½ sangue Angus foram submetidos a três tratamentos diários durante 105 dias: controle (n=6), Lev4g (dieta com 4 g de levedura por animal dia-1, n=6) ou Lev 7g ( dieta com 7 g de levedura por animal dia-1, n=6). Nos dias 29 e 90 após a entrada no confinamento, coletou-se amostras de fezes, swab de pele, água e alimento para contagem de Escherichia coli e coliformes totais e isolamento de Salmonella spp. No dia do abate, realizou-se coleta nas carcaças para identificação de Salmonella spp e quantificação de Escherichia coli, coliformes totais, mesófilos. Notou-se redução de Escherichia coli e coliformes totais nas amostras de fezes (P=0,0001 e 0,001 respectivamente) e de Escherichia coli, coliformes totais e aeróbios mesófilos na carcaça (P=0,06; 0,10 e 0,05 respectivamente) nos grupos tratados. Houve ausência de Salmonella spp. em todas as coletas realizadas. Concluiu-se que a suplementação reduziu a excreção fecal e consequentemente, a contaminação da carcaça pela Escherichia coli, mesófilos e coliformes totais dos animais confinados, sendo uma medida sanitária benéfica e livre de resíduos para melhorar a qualidade microbiológica da carne.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43769099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jenny Paola Hidalgo Martínez, Alexia Brauner de Mello, M. Giacometi, R. C. Cunha, C. B. Oliveira, M. Cleff, R. França
{"title":"Investigation of Trichomonas gallinae in passerines","authors":"Jenny Paola Hidalgo Martínez, Alexia Brauner de Mello, M. Giacometi, R. C. Cunha, C. B. Oliveira, M. Cleff, R. França","doi":"10.5433/1679-0359.2023v44n1p113","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p113","url":null,"abstract":"Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Columbidae family, which have a high prevalence of the protozoan without manifesting the disease. The continuous growth of the pigeon population and its cosmopolitan nature mean that today there is a worldwide distribution of this species, being responsible for the distribution and maintenance of the prevalence of trichomoniasis in almost the entire world. The transmission of the disease may be by direct contact, or indirect, through food or water. This indirect route is the reason why such a wide range of bird orders can be infected, very different from columbids such as falconiformes, strigiformes, passerines, piciformes, psittaciformes, gruiformes, galliformes or anseriformes. Thus, the objective of this study was to evaluate the presence of T. gallinae in passerines received at a Wild Animal Screening Center. In order to carry out this study, 300 birds of the order Passerine corresponding to 23 different species were analyzed, received at the Wild Fauna Rehabilitation Center (NURFS) of the Federal University of Pelotas (UFPel), in different seasons of the year between the months of March to October 2021. Samples of swabs from the oropharynx were collected from all individuals, the material was immediately placed in a falcon tube containing Trypticase-Yeast Extract-Maltose (TYM) culture medium and sent to the Laboratory of Protozoology and Entomology (LAPEn), for incubation in a bacteriological growth greenhouse and subsequent identification of the protozoan on a slide in wet mounting under an optical microscope in a 40X objective. Wet mounting on a slide was performed in triplicate and analyzed in its entirety. Of the 300 birds evaluated in in vitro culture for T. gallinae, 25 had inconclusive results and were submitted to PCR analysis, being negative for T. gallinae. Although no positive Passeriformes was found, the monitoring of the occurrence of this protozoan must continue, as it is known that it may easily cause a possible epidemic, leading to losses for the wild fauna that has endangered birds.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46165386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. M. Gheller, G. C. D. Silva, W. Silva, Aldair Félix da Silva, L. C. C. Costa Filho, É. Nogueira, F. A. M. Melo Sterza
{"title":"Antral follicle count classification selects fertile heifers without influencing the carcass traits","authors":"J. M. Gheller, G. C. D. Silva, W. Silva, Aldair Félix da Silva, L. C. C. Costa Filho, É. Nogueira, F. A. M. Melo Sterza","doi":"10.5433/1679-0359.2023v44n1p85","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p85","url":null,"abstract":"This study aimed to evaluate the ovarian structure, estrus intensity, ultrasound carcass measurements, and pregnancy rate of Nelore breed heifers and cows in accordance with antral follicle counts (AFCs). We evaluated 503 heifers and 565 Nelore cows, with a mean age of 15.5±2.2 and 69.8±36.1 months, respectively, submitted to a fixed-time artificial insemination (FTAI) protocol. On day zero, all bovine females were examined using ultrasound to determine the AFC. The mean AFC of the heifers and cows were 20±8.6 and 22.5±8.4, respectively. The rib-eye area (REA) and fat thickness (FT) of the heifers (n = 119 for REA and n = 219 for FT) were measured using ultrasound imaging. The average conception rates at the first FTAI and at the end of the breeding season were 35.8% and 57.5%, respectively, for heifers and 45.1% and 78.9%, respectively, for cows. We demonstrated that the probability of pregnancy at the first FTAI and at the end of the breeding season for both young heifers and cows increased as the AFC decreased (P>0.001 and P=0.0123, respectively). FT and REA showed no correlation with AFC in heifers. The intensity of estrus expression was negatively correlated with AFC (−0.46; P<0.0001). In conclusion, Nelore heifers and cows with low AFC had a high probability of pregnancy during the entire breeding season. Thus, AFC can be used as a tool to select heifers with increased fertility without affecting carcass traits (REA and FT).","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43552127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Janaina Martins de Medeiros, Luana de Bittencut Acosta, L. I. Schneider, A. Borba, D. Klein, M. Migliorini, H. C. M. Muniz, V. de Oliveira, C. E. Gewehr
{"title":"Performance and behavior of piglets fed diets with different metabolizable energy levels","authors":"Janaina Martins de Medeiros, Luana de Bittencut Acosta, L. I. Schneider, A. Borba, D. Klein, M. Migliorini, H. C. M. Muniz, V. de Oliveira, C. E. Gewehr","doi":"10.5433/1679-0359.2023v44n1p73","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p73","url":null,"abstract":"This study evaluated the effect of different metabolizable energy (ME) levels in diets on digestibility, performance, and feeding behavior of weaned piglets. A digestibility study to determine ME levels was performed using 12 male piglets with 11.5 ± 0.5 kg body weight (BW), in a cross-over design fed with different ME levels (treatments). In the performance study were used 64 female piglets with 7.5 ± 0.8 kg BW, in a randomized block design with four treatments (3.30, 3.40, 3.50, and 3.60 Mcal.kg-1ME levels), and feeding program with three phases (pre-initial I, pre-initial II, and initial). For feeding behavior, four pens of each treatment were monitored with cameras. The crude-protein digestibility coefficient reduced as dietary ME level increased (P <0.05). In pre-initial I animal performance was not influenced (P <0.05) by ME diet levels, and in the pre-initial II and initial phases, increases in ME caused quadratic (r² 0.99) and linear (r² 0.99) effects on daily feed intake, respectively. When ME levels increased, feed conversion ratio decreased linearly in the pre-initial II phase (r² =0.98), and quadratically in the initial phase (r²= 0.99). The number and duration of feeder visits linearly decreased as the diet energy levels increased (P <0.05). Weaned piglets can regulate feed intake according to dietary ME levels. The performance of weaned piglets can be maintained using diets containing metabolizable energy levels between 3.30 at 3.60 Mcal.ME.kg-1 if the ratio of nutrients to energy is maintained constant.The feed intake behavior of weaned piglets is influenced by increases in dietary metabolizable energy levels evaluated, resulting in fewer and shorter visits to the feeder.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45281655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isabelli Sayuri Kono, Amanda Bertão-Santos, Douglas Aparecido Silva, Roberta Lemos Freire, I. T. Navarro, Pablo Menegon Castilho, Eloiza Telles Caldart
{"title":"Distribuição geográfica sul brasileira de Migonemyia migonei (Diptera: Psychodidae), um vetor putativo de Leishmania infantum (Kinetoplastida: Trypanosomatidae): uma revisão sistemática","authors":"Isabelli Sayuri Kono, Amanda Bertão-Santos, Douglas Aparecido Silva, Roberta Lemos Freire, I. T. Navarro, Pablo Menegon Castilho, Eloiza Telles Caldart","doi":"10.5433/1679-0359.2023v44n1p61","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p61","url":null,"abstract":"Esta revisão sistemática reuniu informações sobre a distribuição espacial no sul do Brasil do flebotomíneoMigonemyia migonei, um possível vetor de espécies de Leishmania causadoras da leishmaniose visceral(LV). Os artigos foram pesquisados nas bases de dados PubMed, Scielo, Web of Science e Scopus usandoas palavras-chave: “Migonemyia migonei AND Paraná”, “Migonemyia migonei AND Santa Catarina”,“Migonemyia migonei AND Rio Grande do Sul”, “phlebotomine AND Parana” , “flebotomíneo AND Paraná”e “flebotomíneo AND Paraná”, “flebotomíneo AND Santa Catarina;”, “flebotomíneo AND Santa Catarina”e “flebotomíneo AND Santa Catarina”, “flebotomíneo AND Rio Grande do Sul;”, “flebotomíneo AND RioGrande do Sul” e “sandfly AND Rio Grande do Sul”. A busca inicial identificou 322 artigos que atenderam aoscritérios de seleção. Em seguida, arquivos vazios ou duplicados foram excluídos. Os títulos foram triadose os textos completos foram obtidos. Esta revisão incluiu 36 artigos, abrangendo 72 dos 399 (18,04%)municípios do Paraná, um dos 295 (0,33%) de Santa Catarina, dois dos 497 (0,40%) do Rio Grande do Sul.Mg. migonei foi encontrado em 54 municípios do Paraná estado, em um município de Santa Catarina e emum do Rio Gr ande do Sul. Com base na ampla distribuição de Mg. migonei nos municípios paranaenses,é necessária maior atenção quanto aos casos de LV em humanos e animais, além de investigaçõesepidemiológicas desses casos de suspeita de autoctonia, bem como maiores esforços de prevenção econtrole. Mais estudos são necessários em Santa Catarina e Rio Grande do Sul.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46795119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gustavo Roberto Dias Rodrigues, Marco Túlio Santos Siqueira, Naiara Cristina dos Santos Silveira, Marcela de Sousa Coelho, G. L. M. Macedo Júnior, A. H. Gameiro, Natascha Almeida Marques da Silva, C. Raineri
Hugo Richard Dyck, J. H. Perotta, Taís Casonato Rodrigues, J. A. Galvão, J. Brum, I. R. B. Barros Filho
{"title":"Occurrence of abomasal displacement in dairy cows from High-yielding dairy farms of Paraná State, Southern Brazil","authors":"Hugo Richard Dyck, J. H. Perotta, Taís Casonato Rodrigues, J. A. Galvão, J. Brum, I. R. B. Barros Filho","doi":"10.5433/1679-0359.2023v44n1p9","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p9","url":null,"abstract":"The present study aimed to verify the occurrence of displacement of the abomasum (DA) in dairy herds from a high-yielding dairy region of Southern Brazil. Data on breed, age, lactation number, days after calving, breeding system, number of animals, and number of lactating cows in the herd were obtained from 135 cases of DA. A total of 39 herds, and 6,454 cows, including 2,987 lactating cows from the municipality of Palmeira, Paraná State, were included in this study. The overall prevalence of DA was 2.09%, and occurrence of DA during lactation was 4.42%. Left displacement was more prevalent, with 94.07% of the cases. The mean number of lactations and age of the cows were 2.5±1.16 lactations and 50.9±18.5 months, respectively. DA occurred predominantly in the semi-intensive breeding system (68.1% of cases), during the first 4 weeks postpartum (84.4% of cases), in Black and White Holstein-Friesian cows (94.07% of cases), and during the winter (31.1% of cases). Cows with DA in high-yielding dairy farms in Southern Brazil were similar to cows from herds of high-milk-yielding regions of North America and Europe. Multiparous Black and White Holstein-Friesian cows showed the highest prevalence of DA during the transition period, mostly on the left side.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45973328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dislaine Becker, D. Paulus, L. C. Bosco, E. T. D. Hojo, A. J. Sato, L. Yamamoto, G. Ritter, G. A. Nava
{"title":"Validation of the PhenoGlad model and determination of planting dates of the gladiolo for the state of Paraná","authors":"Dislaine Becker, D. Paulus, L. C. Bosco, E. T. D. Hojo, A. J. Sato, L. Yamamoto, G. Ritter, G. A. Nava","doi":"10.5433/1679-0359.2023v44n1p39","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n1p39","url":null,"abstract":"Mathematical models that simulate crop development are important tools to help growers plan management practices and harvest time for cut flowers such as gladiolus. The objective of this work was to validate the PhenoGlad model for the State of Paraná, determining the planting date for marketing flowers for the Day of the Dead, and the quality of the floral stems. The validation of the PhenoGlad model was carried out through field tests in five towns in the State of Paraná, namely: Cascavel, Dois Vizinhos, Marechal Cândido Rondon, Palotina, and Santa Helena. The experiments were carried out during the year 2019. The PhenoGlad model was run for each location and year of meteorological data for the early, The PhenoGlad model was simulated for each location and period of meteorological data available at each weather station in these towns, and for the early, intermediate II, and late development cycles. The planting date was determined through the average of the dates (Julian days) for each simulated year. The variables analyzed were the stages of development according to the phenological scale of the culture, the number of leaves, the average number of florets, and the classification of floral stems according to the quality standards of Veilling Holambra. The PhenoGlad model showed accuracy in simulating the stages of development of gladiolus culture in the state of Paraná and is also suitable for predicting the damage caused by extreme temperatures in floral stems, which was confirmed by the damage caused to sepals and petals of gladiolus in field experiments in the towns of Cascavel, Dois Vizinhos, Palotina, and Santa Helena. The best model performance was observed when simulating the vegetative period of the crop, presenting a low error of 0.54 leaf. In the state of Paraná, for the production of floral stems for the Day of the Dead, the PhenoGlad model simulated the planting dates in the different towns studied from August 1 to August 23. The towns of Cascavel and Marechal Cândido Rondon presented a higher number of florets and higher quality of floral stems than the other towns evaluated.","PeriodicalId":21663,"journal":{"name":"Semina-ciencias Agrarias","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70687155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}