Seed Science and Technology最新文献_第8页

Development and validation of a direct PCR based assay for the detection of Colletotrichum species on chili seeds 辣椒种子炭疽病菌直接PCR检测方法的建立与验证

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.1.12

S. Dhiman, N. Kumari, A. Badiyal, Vivek Sharma, P. Sharma

{"title":"Development and validation of a direct PCR based assay for the detection of Colletotrichum species on chili seeds","authors":"S. Dhiman, N. Kumari, A. Badiyal, Vivek Sharma, P. Sharma","doi":"10.15258/sst.2022.50.1.12","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.12","url":null,"abstract":"An investigation was executed to detect Colletotrichum truncatum (synonymous C. capsici) and C. coccodes in solo or as a disease complex through direct PCR (dPCR) in anthracnose-infected chili seeds. Direct PCR was performed with C. coccodes and C. truncatum-s specific markers and Tris-EDTA buffer aliquots (obtained from infected seeds soaked up to five hours) as source of template DNA. This method efficiently and specifically detected the respective species in seeds with minimum 2.5% infection, yielding species-specific ∼500 bp (C. truncatum) and ∼340 bp (C. coccodes) fragments without any non-specific amplification with other mycoflora. Further, the seeds used in the experiment were tested for their germination efficiency along with a complete set of dried seeds as control. Among the soaked seeds, germination frequency ranged between 50 (infected seeds) to 100% (healthy seeds) without any significant loss in germination, confirming the sustainability of the current protocol. We recommend the use of direct PCR from soaked seeds without prior DNA extraction as a cost-effective and quick method for detecting the pathogen directly from infected seeds in fields.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Exposing Viburnum edule seeds to a sequence of temperatures affects the germination characteristics 将荚蒾种子暴露在不同的温度下会影响其萌发特性

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.2.07

Mark Baah-Acheamfour, J. Sobze

引用次数: 0

Invited Review: Seed traits inform the germination niche for biodiverse ecological restoration 种子性状为生物多样性生态恢复提供萌发生态位信息

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.1.s.06

Emma L. Dalziell, Wolfgang Lewandrowski, L. Commander, C. Elliott, T. E. Erickson, E. P. Tudor, S. Turner, D. Merritt

{"title":"Invited Review: Seed traits inform the germination niche for biodiverse ecological restoration","authors":"Emma L. Dalziell, Wolfgang Lewandrowski, L. Commander, C. Elliott, T. E. Erickson, E. P. Tudor, S. Turner, D. Merritt","doi":"10.15258/sst.2022.50.1.s.06","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.s.06","url":null,"abstract":"Seeds are a primary source for generating plants for large-scale restoration and understanding the requirements for seed germination and establishment is fundamental to ecological restoration. Seed germination traits are central to defining the germination niche and identifying traits that may limit seedling establishment provides insights into how seeds may interact with the abiotic environment and the soil substrates specific to each restoration site and informs seed management practices. In this paper we review seed trait data derived from research to improve restoration practice across diverse ecosystems within Western Australia. We compile and evaluate seed trait data for up to 300 seed collections of 287 species that are used in restoration programmes to identify species, lifeform, and region-specific variation in seed dormancy, maximum germination, germination speed, base water potential, and germination niche breadth. Through a synthesis of this data, we outline our ecologically-guided approach to identifying key seed traits that support plant regeneration to improve the success of seed-based restoration across the biodiverse Western Australian landscape.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of antifungal plant extracts on improving maize seedling emergence and plant growth 抗真菌植物提取物对玉米幼苗出苗率和植株生长的影响

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.3.04

Rehema Erasto, R. Madege, N. Kilasi

{"title":"Effects of antifungal plant extracts on improving maize seedling emergence and plant growth","authors":"Rehema Erasto, R. Madege, N. Kilasi","doi":"10.15258/sst.2022.50.3.04","DOIUrl":"https://doi.org/10.15258/sst.2022.50.3.04","url":null,"abstract":"Maize seeds are usually contaminated with seed-borne fungi which cause seed deterioration and seedling death. Seed treatment using chemical fungicides is a common practice, although fungicides can reduce seed longevity and lead to residues, resistance, and environmental pollution. This study was conducted to determine the effects of plant extracts (PEs; Azadirachta indica, Coffea Arabica, and Zingiber officinale) on seedling emergence and seedling vigour of certified (CS) and farmer-saved (FSS) seeds of maize. Seeds treated with water-extracted PEs had significantly higher seedling emergence with 85.8% and 61.7% for FSS and CS, respectively, while ethanol-extracted PEs had 6.7% and 7.5% seedling emergence for FSS and CS, respectively. The minimum mean number of days to the first emergence was less for seeds treated with water-extracted PEs (3.0 and 3.3 for FSS and CS, respectively) than those treated with ethanol-extracted PEs (6.3 and 7.0 for FSS and CS, respectively). According to this study, water-extracted PEs are potential candidates in seed treatment, because they have fewer adverse effects on seedling emergence and vigour. Although other studies mention PEs extracted using organic solvents to be the best, the study’s recommendation is to ensure that organic solvents are completely removed from PE solutions before using them.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of gibberellic acid treatment and alternating temperature on breaking physiological dormancy and germination in Penthorum chinense Pursh (Penthoraceae) 赤霉素酸处理和交变温度对中国草打破生理休眠和萌发的影响

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.2.04

Mi Hyun Lee, C. Song, Chongho Park, K. Song, Soon-Young Kim, Sea Hyun Kim, C. Na

{"title":"Effect of gibberellic acid treatment and alternating temperature on breaking physiological dormancy and germination in Penthorum chinense Pursh (Penthoraceae)","authors":"Mi Hyun Lee, C. Song, Chongho Park, K. Song, Soon-Young Kim, Sea Hyun Kim, C. Na","doi":"10.15258/sst.2022.50.2.04","DOIUrl":"https://doi.org/10.15258/sst.2022.50.2.04","url":null,"abstract":"Penthorum chinense (Penthoraceae) is a threatened South Korean medicinal plant used to treat alcohol-related diseases and prepare cosmetics in traditional Chinese medicine. Wetlands, the native habitat of this plant, have been decreasing owing to damage caused by landisation and urban development in recent years. In this study, we considered the effect of temperature and gibberellic acid treatment on P. chinense seed germination to improve conservation outcomes of the species. The optimum germination temperature was investigated using constant and alternating temperature treatments (60 conditions). In addition, gibberellic acid (GA3) treatment was performed to overcome physiological dormancy. We determined the germination (GP, %) and the number of days to reach 50% maximum germination (T50). The optimum temperature regime was 7°C / 23°C: GP was 95%, and T50 was 15 days. The GA3 treatment increased the GP of P. chinense seeds from 28 to 94% and decreased the T50 value from 14.6 to 6.3 days at concentrations above 40 mg L-1. After the GA3 treatment, the growth of radicles was delayed or inhibited. For the germination of P. chinense seeds, a diurnal temperature difference of ≥ 15°C is required.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The effect of seed priming on the germination properties of Aruncus dioicus 引种对黄穗槐发芽特性的影响

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.2.05

Si-Hong Kim, Su-Yeon Lee, J. Heo

引用次数: 0

Book Review: Advances in Seed Science and Technology for More Sustainable Crop Production 书评:促进可持续作物生产的种子科学技术进展

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.3.11

Michael Sussman

引用次数: 0

Invited Review: Use of the tetrazolium test for estimating the physiological quality of seeds 特邀综述:四氮唑试验在种子生理品质评价中的应用

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.1.s.03

J. França-Neto, F. C. Krzyzanowski

{"title":"Invited Review: Use of the tetrazolium test for estimating the physiological quality of seeds","authors":"J. França-Neto, F. C. Krzyzanowski","doi":"10.15258/sst.2022.50.1.s.03","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.s.03","url":null,"abstract":"The tetrazolium test has been developed and improved since the early years of the 20th century. Hundreds of seed specialists have dedicated their efforts for perfecting the methodology of the test for several species. Among these seeds experts, two of them deserve special recognition, Dr. Georg Lakon and Dr. Robert P. Moore, whose achievements had fundamental importance for the further development of the test. The test relies on the activity of certain dehydrogenase enzymes and indirectly measures the respiration capability of the living tissues of the seeds. The initial objective of the test was to quickly determine seed viability. Later on, it focussed on estimating seed vigour and more recently in diagnosing the possible causes that contribute to reduce the physiological quality of seed lots, such as mechanical damage, weathering damage and insect problems. Nowadays, the methodology of the tetrazolium test has been perfected for seeds of several species, including agricultural, vegetable, horticultural, flower, tree and shrub species. Seed quality control is improved with the use of the tetrazolium test in all phases of seed production such as harvesting, receiving, before and after seed processing and drying, during storage and before sowing. The test can be applied even before harvesting the crop. The methods for determining vigour will be briefly presented for seeds of the following species: cotton, common bean, maize, soybean, wheat, sunflower, peanut, Solanaceae, and tropical forage grass seeds. The utilisation of tetrazolium results, as with any vigour test, can assist the categorisation of vigour levels for different seed lots and for estimating the performance of these lots in the field under optimum and stressful conditions.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"461 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Evaluation of dormancy breaking treatments for enhanced germination in Cotinus coggygria, Cornus mas and Viburnum lantana seeds 破休眠处理对山茱萸、山茱萸和紫荚苋种子萌发促进作用的评价

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.3.03

R. Moghimifam, A. R. Haghighi

引用次数: 0

Determination of hybrid-seed genetic purity in wax gourd using newly developed InDel markers based on whole-genome resequencing 基于全基因组重测序新开发的InDel标记测定冬瓜杂交种遗传纯度

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2022-01-01 DOI: 10.15258/sst.2022.50.2.10

Haixuan Lv, Liwen Su, Wenting Wu, Xiaochun Huang, Ting Liu, Lifeng Nong, Wenrui Yang, Yong Chen, Peng Wang, Zhengguo Liu

{"title":"Determination of hybrid-seed genetic purity in wax gourd using newly developed InDel markers based on whole-genome resequencing","authors":"Haixuan Lv, Liwen Su, Wenting Wu, Xiaochun Huang, Ting Liu, Lifeng Nong, Wenrui Yang, Yong Chen, Peng Wang, Zhengguo Liu","doi":"10.15258/sst.2022.50.2.10","DOIUrl":"https://doi.org/10.15258/sst.2022.50.2.10","url":null,"abstract":"Wax gourd (Benincasa hispida) is highly appreciated for its nutritional and therapeutic value, especially throughout Asia. As the market for wax gourd expands, new varieties of unknown purity are being promoted. In this study, the unpublished ‘GX-19’ wax gourd genome elucidated by our research group was used as reference genome. Two high-generation inbred lines (‘HX-1’ and ‘HX-6’) were resequenced and 330 pairs of InDel primers were designed and synthesised. Following screening, 11 pairs of InDel markers were identified and used to determine the degree of genetic purity of wax-gourd hybrid seeds. InDel molecular markers were used to identify the purity of 17 hybrids, including ‘Lvxianzi 2’, ‘Fenxianzi 1’, ‘Jinyuan 1’, ‘Yinyuan 4’, ‘Chunfeng 868’, ‘Xinjinyu’ and ‘Meihua 8’. Additionally, we showed that the amplification fragment from DNA extracted using the alkali rapid-extraction method developed in this study was identical to that of the fragment amplified from DNA extracted using a modified CTAB method. When the InDel molecular marker-based purity determination of five wax gourd hybrids was compared with that using the growing out test, the results were highly consistent. A stable, accurate, efficient and straightforward approach is provided to determine the genetic purity of hybrid wax gourd seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1