Seed Science and Technology最新文献_第2页

Seed Science and Technology: Biology, Production, Quality 种子科技:生物学、生产、品质

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.1007/978-981-19-5888-5

引用次数: 4

L-DOPA assay of polyphenol oxidase (PPO) for varietal identification in rice (Oryza sativa L.) 水稻多酚氧化酶(PPO)的L-DOPA测定及其品种鉴定

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.1.05

S. Kumar, S. Chakrabarty

{"title":"L-DOPA assay of polyphenol oxidase (PPO) for varietal identification in rice (Oryza sativa L.)","authors":"S. Kumar, S. Chakrabarty","doi":"10.15258/sst.2023.51.1.05","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.05","url":null,"abstract":"A phenol colour response test on the grain is used to identify varieties of wheat and rice. It is a harmful, damaging procedure due to the carcinogenic properties of phenol. The activity of polyphenol oxidase (PPO) provides the basis for the phenol colour reaction. The goal of this research was to assess a method similar to the phenol colour reaction, by determining where the polyphenol oxidase activity threshold lies for classifying rice types into different groups. The new PPO assay was applied to commercially available rice varieties with the intention of classifying those that ranged from extremely high to extremely low PPO levels into manageable categories. Substrates such as phenol, L-tyrosine, catechol and 3,4-dihydroxyphenylalanine (L-DOPA) were tested. Twenty cultivars cultivated under identical conditions were put through a screening using a standard assay [1.5 mL of 10 mM L-DOPA in 50 mM 3-(N-morpholino) propane sulphonic acid (MOPS) buffer, pH 6.5, with 3 to 5 seeds constantly rotated in a 2-mL microcentrifuge tube for 0.5 or 1 hour at room temperature]. At 30 minutes, PPO levels were assessed. Based on the PPO values, the varieties belonging to phenol colour groups such as black, dark brown, brown, light brown or no colour matched perfectly. Considering easiness in estimation and other advantages of PPO activity, L-DOPA is proposed as a means of identifying rice varieties instead of the phenol colour reaction test.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Change in the germination performance of Tamarix ramosissima seeds following storage 柽柳种子贮藏后萌发性能的变化

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.1.10

Ruiqi Zheng, Zeming Xing, Qiyu Fu, Xiangfu Shi, Lei Wang

引用次数: 0

Book Review. Seeds of hope: Unearthing the potential of plant regeneration in a warming world 书评。希望的种子:在变暖的世界中发掘植物再生的潜力

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.2.04

Jiezhang Xu, Li Fu

引用次数: 0

Low concentration of nitrate increases germination velocity of intact seeds of Nepeta micrantha in the first few days of germination 低浓度硝酸盐可提高薇甘菊完整种子在萌发初期的萌发速度

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.03

Zeming Xing, Ruiqi Zheng, Xiangfu Shi, Lei Wang

引用次数: 0

The role of skill in successful germination of Melia volkensii seeds 技巧在苦楝种子成功发芽中的作用

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.2.10

E. O. Olung’ati, Benard Kamondo, Millicent Samoei Jepchumba, J. Njuguna, J. Kimondo, J. Kariuki, Ezekiel Kyalo, Naomi Masecha, Phidelis Ntalalai, A. Wafula, Lemaiyan Denis Leparakuo, Lydiah Khaemba

{"title":"The role of skill in successful germination of Melia volkensii seeds","authors":"E. O. Olung’ati, Benard Kamondo, Millicent Samoei Jepchumba, J. Njuguna, J. Kimondo, J. Kariuki, Ezekiel Kyalo, Naomi Masecha, Phidelis Ntalalai, A. Wafula, Lemaiyan Denis Leparakuo, Lydiah Khaemba","doi":"10.15258/sst.2023.51.2.10","DOIUrl":"https://doi.org/10.15258/sst.2023.51.2.10","url":null,"abstract":"Melia volkensii (Melia), locally known as Mukau in Kenya, is an important indigenous dryland forestry species. The species is propagated primarily through seeds, with germination percentage ranging from 40-90%. Successful germination of seeds of forestry species requires skillful application of appropriate pre-sowing treatments. Studies that document and quantify the effect of skill/experience on the germination outcomes for tree seeds are rare. This study sought to fill this gap by documenting the results from a capacity building exercise on testing M. volkensii seeds. Seeds were collected from the KEFRI Kibwezi seed orchard, pretreated and sown by seven participants with different skill levels based on years of experience. The seeds were sown in glasshouse and nursery conditions. There were significant differences (p < 0.001) in germination capacity, mean germination time, T50, mean germination rate, mean daily germination (MDG) and peak value (PV) depending on the skill level of the participants. The results also varied between germination environment (p < 0.001) for all germination traits except MGD and T50. The highest germination outcomes were recorded for participants with the longest experience in testing of M. volkensii seed germination. These findings therefore signify the importance of skill/experience in germination of M. volkensii seeds, an outcome that may have implications for other forestry seeds with special pre-sowing requirements.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67092496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A seed vigour test based on radicle emergence during germination at 5°C for four forage species 4种牧草种子在5℃萌发过程中胚根出芽的活力测定

4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.08

Yaqi Zhang, Qibo Tao, Ruizhen Zhang, Yule Ma, Jiayi Xing, Shenghui Zhou, Yuhe Liu, Fansheng Meng, Juan Sun

{"title":"A seed vigour test based on radicle emergence during germination at 5°C for four forage species","authors":"Yaqi Zhang, Qibo Tao, Ruizhen Zhang, Yule Ma, Jiayi Xing, Shenghui Zhou, Yuhe Liu, Fansheng Meng, Juan Sun","doi":"10.15258/sst.2023.51.3.08","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.08","url":null,"abstract":"Forage species play important role in both livestock production and environment protection. Seed vigour is a critical aspect of seed quality. The objective of this study was to develop a seed vigour test based on radicle emergence at a low temperature for four important forage species, Lolium multiflorum , Coronilla varia , Astragalus sinicus and Vicia sativa . The standard germination test (20&deg;C) (SG) and cold radicle emergence (CRE) to &ge; 2 mm during germination at 5&deg;C were determined for seed lots of four species in the laboratory. Field emergence (FE) was also measured, in April 2022. For four species, the SG of seed lots were high and similar, and was not significantly related to FE. However, the CRE counts at 11, 14, 18 and 7 days from sowing for L. multiflorum , C. varia , A. sinicus and V. sativa , respectively, were well correlated with FE, with coefficients of determination ( R 2 ) of 0.757, 0.815, 0.774 and 0.812, respectively ( P &lt; 0.01). These findings suggested that CRE during germination at 5&deg;C has good potential in assessing FE and hence seed vigour for four forage species.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135701233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invited Review: Climate change impacts on seed production and quality: current knowledge, implications, and mitigation strategies 特邀审查:气候变化对种子生产和质量的影响:目前的知识、影响和缓解战略

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.1.07

A. Maity, Debashis Paul, A. Lamichaney, Abhradip Sarkar, Nidhi Babbar, Nandita Mandal, Suman Dutta, P. Maity, S. K. Chakrabarty

{"title":"Invited Review: Climate change impacts on seed production and quality: current knowledge, implications, and mitigation strategies","authors":"A. Maity, Debashis Paul, A. Lamichaney, Abhradip Sarkar, Nidhi Babbar, Nandita Mandal, Suman Dutta, P. Maity, S. K. Chakrabarty","doi":"10.15258/sst.2023.51.1.07","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.07","url":null,"abstract":"Climate change is real and inevitable, incessantly threatening the terrestrial ecosystem and global food security. Although the impacts of climate change on crop yield and the environment have received much attention in recent years, there are few studies on its implications for the production of high-quality seeds that provide the basic input for food production. Seeds are the primary planting material for crop cultivation and carry most new agricultural technologies to the field. Climatic abnormalities occurring at harvest and during the post-harvest stages may not always severely impact seed yield but can reduce the morphological, physiological and biochemical quality, ultimately reducing the field performance and planting value of the seed lot. In our preliminary data mining that considered the first 30 species appearing in the search results, seed setting, seed yield and seed quality parameters under temperature, CO2 and drought stresses showed differential response patterns depending on the cotyledon number (monocots vs. dicots), breeding system (self- vs. cross-pollinated), life cycle (annual vs. perennial) and maturity time (seed setting in cooler vs. hotter months). The relative proportions of the 30 species showed that germination and seedling vigour are adversely affected more in dicots and self-pollinated annual species that set seeds in hotter months. Together, these impacts can potentially reduce the quantity and quality of seeds produced. Immediate attention and action are required to understand and mitigate the detrimental impacts of climate change on the production and supply of high-quality seeds. This review summarises the current knowledge on this aspect, predicts the future implications and suggests some potential mitigation strategies in the context of projected population growth, climate change and seed requirement at the global level.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De-polymerisation and hydrolysis of seed storage protein is involved in the freezing tolerance of hydrated Brassica oleracea seeds 种子贮藏蛋白的解聚合和水解与含水甘蓝种子的抗冻性有关

4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.11

Yingying Han, Liangbo Li, Weijie Li, Xiuli Wang, Baolin Liu

{"title":"De-polymerisation and hydrolysis of seed storage protein is involved in the freezing tolerance of hydrated <i>Brassica oleracea</i> seeds","authors":"Yingying Han, Liangbo Li, Weijie Li, Xiuli Wang, Baolin Liu","doi":"10.15258/sst.2023.51.3.11","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.11","url":null,"abstract":"Hydrolysis of globulins might be involved in the freezing tolerance of hydrated lettuce seeds. The mechanism was further explored in hydrated brassica ( Brassica oleracea ) seeds, by application of 5,5&apos;-dithiobis(2-nitrobenzoicacid) (DTNB), the Ellman’s reagent, which can bind to the free sulphydryl side chain of cysteine. DTNB increased the viability of hydrated brassica seeds after slow cooling (-3&deg;C hour -1 ) to -17&deg;C. Two- dimensional electrophoresis and mass spectra analysis indicated the accumulation of small peptides derived from 12S globulins (cruciferin) in DTNB-treated seeds after slow cooling. The seed storage peptides with different molecular weight were cooled and the ice formation during cooling observed by cryomicroscopy, which proved that peptides of smaller molecular size produced smaller and rounder crystals. The smallest peptide in DTNB-treated seeds produced the smallest and roundest crystals during cooling. The levels of ASA, DHA and GSH were specifically up-regulated after DTNB treatment and following slow cooling, which indicates that DTNB enhanced the circulation of ASA and GSH to resist the oxidative stress. Consistent with this finding, RNA-seq and real time PCR indicated that glutathione reductase (GR) was up-regulated after DTNB treatment.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136204435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seed viability constants and the decline in germination rates for radish: a combined viability equation and population-based threshold model analysis 萝卜种子活力常数与发芽率下降:一个结合活力方程和种群阈值模型分析

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.05

N. B. Coronado, Renerio P. Gentallan Jr., F. R. Hay, R. Madayag, E. B. Timog, K. J. Quiñones, M. Bartolome, L. E. Endonela, N. C. Altoveros, T. Borromeo

{"title":"Seed viability constants and the decline in germination rates for radish: a combined viability equation and population-based threshold model analysis","authors":"N. B. Coronado, Renerio P. Gentallan Jr., F. R. Hay, R. Madayag, E. B. Timog, K. J. Quiñones, M. Bartolome, L. E. Endonela, N. C. Altoveros, T. Borromeo","doi":"10.15258/sst.2023.51.3.05","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.05","url":null,"abstract":"Aside from the leaves and roots, radish seedlings are consumed as a “microgreen”. Production of microgreens requires high viability, high vigour seeds. Knowledge of the rates of loss of viability and vigour decline during storage would therefore be useful for radish sprout production, but also in relation to the conservation of radish germplasm in seed genebanks. Seeds of radish ‘Kaiware’, a microgreen cultivar, were stored hermetically at seven moisture levels between 6% and 13% at 50°C. Seed viability (germination after removal from storage) was scored daily to determine germination progress curves. One-step analysis was used to simultaneously fit the population-based threshold (PBT) model describing the decline in germination rates with ageing and to estimate the effects of MC on longevity according to the viability equation. KE and CW were estimated as 6.54 and 3.22, respectively. The ageing-time constant for the PBT model, θage, increased as a function of storage moisture content, reflecting the faster decline in vigour at higher MC. The validity of the viability constants was tested by predicting viability loss in seeds of a different radish cultivar, ‘Sango Purple’, stored at 45°C and 8.61% MC.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67092205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0