Yomna F Metwally, Rasha F Zahran, Rana R Elsadda, S. Refaat, Afaf M. Elsaid
{"title":"Association of IL-6 rs1800795 and IL-1 β rs16944 polymorphisms with non-small cell lung cancer in the Egyptian population: a pilot study","authors":"Yomna F Metwally, Rasha F Zahran, Rana R Elsadda, S. Refaat, Afaf M. Elsaid","doi":"10.21608/sjdfs.2023.248290.1144","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.248290.1144","url":null,"abstract":"Lung cancer is a serious health and life issue, with the highest rates of incidence and mortality in the world. It is now clear that inflammation is a key factor involved in all aspects of carcinogenesis, notably lung cancer development. Genetic changes, including polymorphisms in inflammatory genes, are supposed to play a significant role in increasing lung cancer risk. In this study, we aim to investigate the association of IL-6 rs1800795 and IL-1β rs16944 polymorphisms with non-small cell lung cancer (NSCLC) development in the Egyptian population. The study design was composed of 100 NSCLC cases and 100 controls, which were genotyped using the ARMS-PCR technique, electrophoresed on a 2.5% agarose gel, and visualized using ethidium bromide under ultraviolet illumination. The IL-1β rs16944 genotypes were significantly different in NSCLC patients as compared to healthy controls ( p = 0.032). Whereas the genotypes and alleles of the IL-6 rs1800795 were not significantly linked to NSCLC incidence ( p = 0.726; p = 0.822, respectively). To our best knowledge, this study aimed to uncover the great impact of IL-1β rs16944 genotypes on NSCLC development in the Egyptian population; thus, it may be a gateway for earlier NSCLC prevention.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"48 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138981920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Electrochemical sensing of antipyrine and its interaction with ds-DNA modified electrode.","authors":"ahmed ramadan, abdelgawad radi","doi":"10.21608/sjdfs.2023.221623.1116","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.221623.1116","url":null,"abstract":"The interaction of antipyrine (AnP) with the double-stranded deoxyribonucleic acid of salmon sperm on a electrochemical electrode (GCE) was carefully examined using voltammetric methods.The Ultra violet-visible absorption method was used to determine the interaction of AnP and double strand DNA in solution. AnP give a single anodic peak in buffer solutions of (pH 3.5 - 9.5).AnP is oxidized in a diffusion-controlled process from a phosphate buffer solution, pH 7.The variation given by AnP through its current signals was monitored in the existance of DNA by GCE using different voltammetric techniques. Diffusion coefficient of free AnP was 3.7×10 -6 and 2.8×10 -7 cm 2 s -1 for the AnP-DNA .The binding constant was calculated to be 1.5×10 5 and 1.1×10 5 M -1 using DPV and UV / vis Spectroscopy. AnP binds to DNA primarily by electrostatic interaction with a contribution from intercalation. A detection pattern depends on pre-concentration and DPV detection at GCE modified with dsDNA was introduced to determine AnP in saliva matrices.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"637 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138982852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
yahya bdreldien, Mohamed Abou-Dobara, A. El-Sayed, Mohamed Zaghloul
{"title":"Morphological and Ultrastructural Changes of Escherichia coli and Klebsiella pneumoniae carriers of β-lactamase when subject to β-lactam Antibiotic","authors":"yahya bdreldien, Mohamed Abou-Dobara, A. El-Sayed, Mohamed Zaghloul","doi":"10.21608/sjdfs.2023.230321.1127","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.230321.1127","url":null,"abstract":"In our manuscript, we examine the structure and morphology, we take an imperative look at this pathway. We collected clinical isolates from patients at the Oncology Center of Mansoura University, from urine samples. The isolates were then screened for antibiotic resistance and beta-lactamase enzyme production, with a focus on the most prevalent bacterial isolates. To further investigate the impact of one particular beta-lactam antibiotic on Escherichia coli and Klebsiella pneumoniae , both known producers of the beta-lactamase enzyme, we utilized Transmission Electron and Scanning Electron Microscopes. Our study aims to discuss the various changes induced by cefoxitin, including its effects on the characteristics of clinical isolates such as the cell wall structure of E. coli and K. pneumoniae . The aim of this manuscript is to discuss the occurrence of alterations caused by beta-lactam antibiotics. Photomicrographs will be used to describe these alterations and provide information about the agents responsible for the changes. We will focus on the structural and morphological changes that are consistently observed.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"71 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138982990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amira El-Fallal, T. El-Katony, Heba Dahap, H. El-Gharabawy
{"title":"Effect of phosphorus form and culturing mode on mycelial growth of Pleurotus pulmonarius and Pleurotus floridanus on rice straw","authors":"Amira El-Fallal, T. El-Katony, Heba Dahap, H. El-Gharabawy","doi":"10.21608/sjdfs.2023.222643.1118","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.222643.1118","url":null,"abstract":"Oyster mushroom ( Pleurotus spp.) cultivation gained a growing concern due to their limited growth requirements and high nutritional value and bioactive compounds. Present work investigates the effect of rice straw supplementation with inorganic P (KH 2 PO 4 ) and organic P (wheat bran) with presence or absence of agar layer above straw on mycelial growth of two oyster mushrooms: P. pulmonarius and P. floridanus. Mycelial growth was better with bran than with KH 2 PO 4 , particularly without agar. Such a differential effect of P form and agar layer was more evident on P. pulmonarius than on P. floridanus . Mycelial growth mostly exhibited a saturable dose-dependent pattern and increased up to a certain optimal P supply of 2–5 g P/g straw, post which it approached a plateau; only in the no agar-bran culture of P. floridanus a progressive P-dependent increase was found. The time-course of fungal growth exhibited contrasting patterns in the two fungi; being sigmoidal in P. pulmonarius but almost linear in P. floridanus . In P. pulmonarius , the lag growth period (T 10 ) was longer with KH 2 PO 4 than with bran, with different dose-dependent patterns in the two P sources and marginal effect of the agar layer. Relative growth rate (RGR) of P. pulmonarius was higher than that of P. floridanus , with different dose-dependent patterns according to fungal species and presence of agar. In P. pulmonarius , both T 10 and RGR attained comparable values of about one day and 0.4 day -1 , respectively at 5 mg P/g straw for the different P form × culturing mode combinations..","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"128 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139010672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
zeinab Abdelsalam, H. Taha, mohamed elbokl, Essam Yasin
{"title":"Effect of Temperature on Biological Aspects, Fecundity and Life Table Parameters of The Predatory Mite, Cheyletus malaccensis","authors":"zeinab Abdelsalam, H. Taha, mohamed elbokl, Essam Yasin","doi":"10.21608/sjdfs.2023.209617.1107","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.209617.1107","url":null,"abstract":"The predatory mite, Cheyletus malaccensis (Oud.) was recorded to be the communist predacious mites of family Cheyletidae, it was found associated with other different mite species and immature stages of insects, which were collected from palm tree fibers in Damietta Governorate. Biological aspects, fecundity and life table parameters of Cheyletus malaccensis were studied under laboratory conditions at 25 and 35 o C and 65% R.H. Feeding on Immatures of Tyrophagus putrescentiae . Obtained results showed that the developmental stages of both female and male were affected by temperature as a vital environmental factor, were as its decreased as well as temperature increased. Also, deposited an average of 98.9 and 57.75 eggs during 54.18 and 13.1 days, with a daily rate of 1.8 and 4.5 eggs at 25 and 35 o C. Life table parameters showed highest value of the net reproductive rate (R o ) as 62.83 at 25 o C. Sex ratios were 0.46 for both temperatures.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"101 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139010698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Toson, Nagwa M. Abdelhadi, Abdel-Aziz F. Abdel-Aziz
{"title":"Oral intake of Pomegranate Peels Stimulate Glutathione levels and Superoxide Dismutase Activity to Protect Against Cisplatin-Induced Nephrotoxicity","authors":"E. Toson, Nagwa M. Abdelhadi, Abdel-Aziz F. Abdel-Aziz","doi":"10.21608/sjdfs.2023.230342.1128","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.230342.1128","url":null,"abstract":"","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"405 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Serag, Reham elbarougy, Hanan Abo Elagras, Abdel-Hamid Khedr
{"title":"Phenotypic plasticity of four invasive species in north-eastern section of the Nile Delta coast, Egypt","authors":"M. Serag, Reham elbarougy, Hanan Abo Elagras, Abdel-Hamid Khedr","doi":"10.21608/sjdfs.2023.223602.1120","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.223602.1120","url":null,"abstract":"One of the essential mechanisms through which plants can adapt to prevailing environmental conditions is phenotypic plasticity. To explain the invasive success across the invaded new areas, the phenological pattern and vegetative features of the exotic species are investigated. The four exotic weeds included in this study are Xanthium strumarium L., Bassia indica (Wight) A. J. Scott, Atriplex lindleyi Moq., and Trianthema portulacastrum L. Results indicated that shoot length was greatest in X. strumarium, followed by B. indica and A. lindleyi but least in T. portuiaeustrum. Fresh weight of the foliage was comparable in X. strumarium and A. lindleyi, being higher in both than in B. indica and T. portulacastrum. Dry wight of the foliage was highest in X. strumarium, followed by A. Lindleyi, but it was least with comparable values of B. indica and T. portulacastrum. Water content of shoots was higher in T. portulacastrum and B. indica, slightly lower in X. strumarium and least in A. lindleyi. The highest concentrations of P (0.620.02), K (0.060.001), and Mg (24.763.79) were found in site 2 of T. portulacastrum. The highest levels of N (6.070.30), Na (0.200.009), and moisture content (44.320.40) were found in site 3 for B. indica. The components with the lowest concentrations were P (0.100.01), K(0.010.0003), Mg (0.370.01), and Na (0.040.003) in A. Lindleyi at site 4, whereas N (2.171.00) and moisture content (17.215.10) were the lowest in X. strumarium at site 1.Analysis of variance revealed significant (P<0.05) to very highly significant (P<0.001) variation in growth parameter among the studied plant species. Based on the magnitude of the F ratio, the variability was most evident in shoot dry weight, followed by shoot fresh weight and water content but was relatively low in number of leaves, number of branches and water content of shoot. Based on the obtained results, it can be concluded that the four exotic weeds can successfully invade new areas by changing their phenological plasticity. The optimal management and control measures of these exotic weeds will benefit from this .","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"259 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"On the generation of ion-acoustic super-periodic waves in ultra-relativistic degenerate quantum plasma","authors":"E. Habbak, M. Selim, S. El-Labany","doi":"10.21608/sjdfs.2023.210193.1108","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.210193.1108","url":null,"abstract":"","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"519 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reham el fanagely, Ayman Hyder, Hekmat Elgammal, Atef Wahdn
{"title":"A simple point score model for prediction of Covid-19 in some Egyptian patients","authors":"Reham el fanagely, Ayman Hyder, Hekmat Elgammal, Atef Wahdn","doi":"10.21608/sjdfs.2023.208364.1105","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.208364.1105","url":null,"abstract":"On a global scale, a substantial number of newly diagnosed cases of the covid-19 virus and a considerable number of associated fatalities are recorded weekly. Its laboratory detection depends on the costly and time-consuming real-time PCR analysis. A simple way to facilitate the diagnosis of Covid-19 is still required. Here, it was aimed to generate a simple point score as a prediction model for fast diagnosis of Covid-19 using simple laboratory analyses. 121 adult individuals with qRT-PCR results served as a training group, whereas 35 individuals were used as a validation group. Different laboratory analyses, including complete blood count (CBC), differential count, D-dimer, C-Reactive Protein (CRP), and Ferritin, have been recruited as predictors using the Receiver Operating Characteristic (ROC) analysis. The results revealed three models, depending on the predictor parameters' ROC area (AUC). The simplest model consisted of the data of the three predictors: lymphocytopenia, CRP, and D-dimer, and resulted in a ROC AUC value of 0.9773. The use of the three models on the validation group provided support for the conclusion that the calculation of lymphocyte count, CRP, and D-dimer is enough for predicting the occurrence of Covid-19.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"313 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amira Abd El-Nour, Mohamed Abou-Dobara, A. El-Sayed, M. El-Zahed
{"title":"Antibacterial activity of optimized extracellular biosynthesized zinc oxide nanoparticles using Corynebacterium sp. ATCC 6931","authors":"Amira Abd El-Nour, Mohamed Abou-Dobara, A. El-Sayed, M. El-Zahed","doi":"10.21608/sjdfs.2023.231788.1129","DOIUrl":"https://doi.org/10.21608/sjdfs.2023.231788.1129","url":null,"abstract":"Nanoparticle (NP) green synthesis is gaining popularity and has been proposed as a potential substitute for chemical and physical processes. The current study reports the environmentally friendly, low-cost method of optimized zinc oxide nanoparticles (ZnO NPs) utilizing a crude metabolite from Corynebacterium sp. ATCC 6931. Aliquot pH 8, 35°C, and an 8:2 (v/v) ratio of bacterial supernatant to zinc nitrate solution were the best conditions for the formation of ZnO NPs. Transmission electron microscopy (TEM), UV-visible spectroscopy (UV-vis), X-ray diffraction (XRD), and Zeta potential analyses were used to characterize the biosynthesized NPs. The synthesized ZnO NPs had sizes ranging from 8 to 17 ± 1.23 nm were mainly spherical in form and had a positive charge of ≈ +18.9 mV. The biosynthesized ZnO NPs in the current study have been applied in antimicrobial applications. Using the agar well diffusion method, different concentrations of biosynthesized ZnO NPs (50 µg/mL, 100 µg/mL, and 150 µg/mL) were used to test the antibacterial activity of the characterized NPs against the Gram-positive and Gram-negative bacteria: Bacillus cereus, Corynebacterium sp., and Escherichia coli. Additionally, the minimum inhibitory concentration (MIC) test was applied, and the results showed that bacterial growth reduces as biosynthesized ZnO NPs concentration increases. Also, compared to Gram-positive bacteria, Gram-negative bacteria appeared to be more sensitive to ZnO NPs.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"42 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}