Morphological and Ultrastructural Changes of Escherichia coli and Klebsiella pneumoniae carriers of β-lactamase when subject to β-lactam Antibiotic

In our manuscript, we examine the structure and morphology, we take an imperative look at this pathway. We collected clinical isolates from patients at the Oncology Center of Mansoura University, from urine samples. The isolates were then screened for antibiotic resistance and beta-lactamase enzyme production, with a focus on the most prevalent bacterial isolates. To further investigate the impact of one particular beta-lactam antibiotic on Escherichia coli and Klebsiella pneumoniae , both known producers of the beta-lactamase enzyme, we utilized Transmission Electron and Scanning Electron Microscopes. Our study aims to discuss the various changes induced by cefoxitin, including its effects on the characteristics of clinical isolates such as the cell wall structure of E. coli and K. pneumoniae . The aim of this manuscript is to discuss the occurrence of alterations caused by beta-lactam antibiotics. Photomicrographs will be used to describe these alterations and provide information about the agents responsible for the changes. We will focus on the structural and morphological changes that are consistently observed.