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Science in China. Series B, Chemistry, life sciences & earth sciences最新文献

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紫外激光诱导M(CO) 6 解离催化聚合端基烯烃和炔烃的研究 紫外激光诱导M(CO) 6 解离催化聚合端基烯烃和炔烃的研究
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-01-10 DOI: 10.1360/ZB1995-25-1-14
林金谷佘永伯傅克坚 张杰, Lin Jin-Gu She Yong-Ba Fu Ke-Jian Zhang Jie
{"title":"紫外激光诱导M(CO) 6 解离催化聚合端基烯烃和炔烃的研究","authors":"林金谷佘永伯傅克坚 张杰, Lin Jin-Gu She Yong-Ba Fu Ke-Jian Zhang Jie","doi":"10.1360/ZB1995-25-1-14","DOIUrl":"https://doi.org/10.1360/ZB1995-25-1-14","url":null,"abstract":"报道了紫外激光诱导金属羰基化合物M(CO) 3 在不同卤代烃中解离进行α-H的烯烃和炔烃多相和均相催化聚合的研究.研究结果表明:W(CO) 6 在CCl 4 中激光解离生成的多相催化剂能有效地催化聚合具有活泼 α -H的端基烯烃;W(CO) 6 在CH 3 I激光解离生成的均相催化剂能进行苯乙炔的均相催化聚合,并且所得到聚合物的结构完全不同于W(CO) 6 在CCl 4 中激光解离多相催化聚合苯乙炔得到的聚合物的结构。此外,还对M(CO) 6 在卤代烃中的解离产物及聚合过程进行了详细的分析和讨论。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"58 1","pages":"14-22"},"PeriodicalIF":0.0,"publicationDate":"1995-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77771177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
捕光蛋白——多管藻中R—藻红蛋白的初步晶体学研究 捕光蛋白——多管藻中R—藻红蛋白的初步晶体学研究
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-01-10 DOI: 10.1360/ZB1995-25-1-49
万柱礼宋海卫张季平汪曙光桂璐璐梁栋材朱晋昌杨紫萱 常文瑞
{"title":"捕光蛋白——多管藻中R—藻红蛋白的初步晶体学研究","authors":"万柱礼宋海卫张季平汪曙光桂璐璐梁栋材朱晋昌杨紫萱 常文瑞","doi":"10.1360/ZB1995-25-1-49","DOIUrl":"https://doi.org/10.1360/ZB1995-25-1-49","url":null,"abstract":"取自青岛海滨的多管藻中的R-藻红蛋白已获得可用于X-射线结构分析用的单晶体.晶体属于 R 3空间群,晶胞参数为 a = b =18.99nm, c =6.04nm,晶体衍射能力优于0.3nm.依据对R-藻红蛋白的初步晶体学分析和晶体有关物理化学性质的测试结果,作者对R-藻红蛋白的亚基构成方式进行了讨论,认为晶体中R-藻红蛋白的亚基组成可能为( αβ ) 6 γ 而不是文献曾报道的( α 2 β ) 3 γ .","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"23 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"1995-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79085490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
p10 genes of Bombyx mori nuclear polyhedrosis virus and Autographa californica multiple nuclear polyhedrosis virus. 家蚕核型多角体病毒和加州签名虫核型多角体病毒的p10基因。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-01-01
Y Zhang, X Wu, Z Li
{"title":"p10 genes of Bombyx mori nuclear polyhedrosis virus and Autographa californica multiple nuclear polyhedrosis virus.","authors":"Y Zhang,&nbsp;X Wu,&nbsp;Z Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>EcoR I-P fragment has been cloned from Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) genomic DNA and used as a probe. 0.5-kb and 1.1-kb fragments including p10 gene from Bombyx mori nuclear polyhedrosis virus (BmNPV) have been hybridized. The p10 ORF was located in the EcoR I-R fragment. Initiation codon ATG of p10 from BmNPV has been mutated by PCR, and the ATG region became a Bgl II site. A novel transfer vector pBmAcPV-1 has been constructed using both the p10 5'-flanking region whose initiation codon ATG has been mutated with BmNPV and the p10 3'-flanking region of AcMNPV. The vector can recombine with not only AcMNPV DNA to express foreign gene in Sf cells, but also BmNPV DNA to express foreign gene in Bm cells. CAT gene was expressed at high level in Bm cells under the control of the mutated p10 promoter of BmNPV.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 1","pages":"50-9"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18698395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure and oxygen sensitivity of nifLA promoter of Enterobacter cloacae. 阴沟肠杆菌nifLA启动子的结构及氧敏感性研究。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-01-01
X Deng, S Shen
{"title":"Structure and oxygen sensitivity of nifLA promoter of Enterobacter cloacae.","authors":"X Deng,&nbsp;S Shen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The nucleotide sequence of the nifLA promoter of Enterobacter cloacae E26 was determined, and the transcription start site of nifLA was mapped by the primer extension. Studies on the oxygen regulation of E. cloacae nifLA promoter with the nifL'-lacZ fusion showed that the nifLA promoter was sensitive to oxygen as Klebsiella pneumoniae nifLA promoter reported previously. Comparison between the nifLA promoter sequences of E. cloacae and K. pneumoniae revealed the presence of an 11-bp conserved sequence between the -24/-12 consensus of sigma 54-dependent promoter and NtrC binding motif. The 11-bp sequence is speculated to be involved in the oxygen regulation of the nifLA promoter of both enteric bacteria.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 1","pages":"60-6"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18698396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using complexity measure to characterize information transmission of human brain cortex. 用复杂性测度表征人脑皮层的信息传递。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1994-12-01
J H Xu, X B Wu
{"title":"Using complexity measure to characterize information transmission of human brain cortex.","authors":"J H Xu,&nbsp;X B Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The information transmission among various parts of the cortex are computed with the theory of mutual information from the data of the electroencephalogram (EEG) time series of normal human subjects. The intensities of these transmissions are characterized by the \"complexity\" measures. These measures have revealed to be sensitively related to the functional conditions of human beings.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"37 12","pages":"1455-62"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18871356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
跨膜Ca 2+ 梯度通过磷脂酰胆碱调节肌质网Ca 2+ -ATP酶 跨膜Ca 2+ 梯度通过磷脂酰胆碱调节肌质网Ca 2+ -ATP酶
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1994-11-10 DOI: 10.1360/ZB1994-24-11-1171
徐红杨福愉 屠亚平, Xu Gong Yang Fu-Tou TU E-Beng
{"title":"跨膜Ca 2+ 梯度通过磷脂酰胆碱调节肌质网Ca 2+ -ATP酶","authors":"徐红杨福愉 屠亚平, Xu Gong Yang Fu-Tou TU E-Beng","doi":"10.1360/ZB1994-24-11-1171","DOIUrl":"https://doi.org/10.1360/ZB1994-24-11-1171","url":null,"abstract":"将纯化的肌质网Ca 2+ -ATP酶重组在具有或不具有跨膜Ca 2+ 梯度的脂质体上,研究了磷脂(特别是磷脂酰胆碱)在跨膜Ca 2+ 梯度对肌质网Ca 2+ -ATP酶功能调节中的作用。结果表明:(1)高浓度的Ca 2+ 对与不同磷脂保温的去脂Ca 2+ -ATP酶都产生抑制,但对与PC保温的Ca 2+ -ATP酶抑制最大。(2)当存在一个正向跨膜Ca 2+ 梯度时(酶活性中心一侧Ca 2+ 浓度较低,类似生理条件),它只对与中性磷脂PC:PE重组的Ca 2+ -ATP酶产生抑制,且PC:PE(摩尔比)为1:1时,抑制程度最大。如以酸性磷脂PS或PG替代PC则均无明显抑制。(3)比较不同脂肪酸侧链的PC的作用表明,当DOPC或DPPC存在时均有明显抑制作用,而DMPC则几乎不表现抑制作用。(4)若Ca 2+ 梯度逆转,则无论是与酸性磷脂或中性磷脂重组的肌质网Ca 2+ -ATP酶都会被抑制。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"33 1","pages":"1171-1177"},"PeriodicalIF":0.0,"publicationDate":"1994-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87919975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment of beta-thalassemia with hydroxyurea (HU)--effects of HU on globin gene expression. 羟基脲(HU)治疗地中海贫血——HU对珠蛋白基因表达的影响。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1994-11-01
S Z Huang, Z R Ren, M J Chen, H P Xu, Y T Zeng, G P Rodgers, F Y Zeng, A N Schechter
{"title":"Treatment of beta-thalassemia with hydroxyurea (HU)--effects of HU on globin gene expression.","authors":"S Z Huang,&nbsp;Z R Ren,&nbsp;M J Chen,&nbsp;H P Xu,&nbsp;Y T Zeng,&nbsp;G P Rodgers,&nbsp;F Y Zeng,&nbsp;A N Schechter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A newly developed method of RT-PCR/competitive PCR for measuring the relative and absolute content of globin mRNAs as well as micro-globin chain biosynthetic assay have been used to study the alterations of globin gene expressions in the patients with beta-thalassemia pre- and post-hydroxyurea (HU) treatment. It was found for the first time that HU had the effect of enhancing beta-globin gene expression in some patients. Two cases with beta-thalassemia who were subjected to HU treatment for over two years showed a marked increase in beta-globin mRNA level and beta-globin chain synthesis, resulting in more effective erythropoiesis and the alleviation of clinical symptoms.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"37 11","pages":"1350-9"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18862345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlation between unfolded states of apocytochrome c and its ability to pass lipid bilayer. 细胞色素c未折叠状态与其通过脂质双分子层能力的相关性。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1994-11-01
X S Wang, J C Tong, X H Han, F Y Yang
{"title":"Correlation between unfolded states of apocytochrome c and its ability to pass lipid bilayer.","authors":"X S Wang,&nbsp;J C Tong,&nbsp;X H Han,&nbsp;F Y Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In contrast to the horse heart apocytochrome c, the chicken heart apocytochrome c underwent a conformational change from random coil to partial folding during a renaturation process. When the apocytochrome horse heart and that of chicken heart c were subjected to a translocation assay in vitro using large trypsin-enclosed unilamellar vesicles from soybean phospholipids, the ability of the chicken heart apocytochrome c to penetrate into the liposomes was found to decrease markedly with the renaturation procedure, while that of horse heart apocytochrome c remained relatively constant. Observations from circular dichroism measurement on the induction of secondary folding of these two species of apocytochrome c upon interaction with soybean phospholipid vesicles suggested that a more flexible structure of apocytochrome c embedded in the lipid matrix be required for its efficient translocation across the bilayer.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"37 11","pages":"1341-9"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18862344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of a 12.5-kD protein from caudate-putamen nucleus as a dopaminergic neuronotrophic factor. 尾状壳核12.5 kd蛋白作为多巴胺能神经营养因子的鉴定。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1994-11-01
M H Zhou, F Ren, L P Zhao
{"title":"Identification of a 12.5-kD protein from caudate-putamen nucleus as a dopaminergic neuronotrophic factor.","authors":"M H Zhou,&nbsp;F Ren,&nbsp;L P Zhao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of the extract from caudate-putamen nucleus of newborn rats (CPe) on the dopaminergic (DA) neurons has been studied. An MTT colorimetric microassay measuring optical densities for the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as compared to those without CPe (p < 0.05). Rhodamine retrograde-prelabelled DA neurons were cultured on a Phastgel containing all the electrophoretically separated protein bands from CPe, and able to fish out from it their own trophic factor, a 12.5-kD protein band. The survived neurons at the 12.5-kD protein band were immunostained positive with anti-dopamine antibody. Co-culturing the 12.5 kD-containing gel strip with explants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neurites outgrowing from the explants only towards the 12.5-kD gel strip. These results indicate that a 12.5-kD protein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promoting their neurite outgrowth.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"37 11","pages":"1360-5"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18862346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial DNA polymorphism in natural populations of Drosophila albomicans (I)--Remarkable mtDNA polymorphism in the population of D. albomicans. 白果蝇自然种群的线粒体DNA多态性(I)——白果蝇种群中显著的线粒体DNA多态性。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1994-11-01
W Wang, F Y Ling, L M Shi
{"title":"Mitochondrial DNA polymorphism in natural populations of Drosophila albomicans (I)--Remarkable mtDNA polymorphism in the population of D. albomicans.","authors":"W Wang,&nbsp;F Y Ling,&nbsp;L M Shi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The technique of mtDNA restriction fragments length polymorphism (RFLP) was used to survey the population structure of D. albomicans. Remarkable mtDNA polymorphism has been observed in D. albomicans populations. A total of 34 nucleomorphs were detected from 82 isofemale lines assayed by only 8 restriction enzymes. The cause and the effect of this phenomenon were discussed. As a result, it is suggested that a mechanism which maintains mtDNA diversity exists in this fly, and that the high intra-populational polymorphism could numerically conceal the extent of differentiation between populations. In addition, on the base of restriction maps, it was found that the mtDNA molecule of D. albomicans might be impacted by the selection pressure during its evolution process both on the nucleotide composition and on the functional regions.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"37 11","pages":"1329-40"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18864461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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