SEPARATION SCIENCE PLUS最新文献_第9页

Development of stability indicating liquid chromatographic method for estimation of novel anti‐diabetic drug Evogliptin 新型抗糖尿病药物依格列汀稳定性指示液相色谱测定方法的建立

SEPARATION SCIENCE PLUS Pub Date : 2023-09-19 DOI: 10.1002/sscp.202300082

Deepanti Gajjar, Dimalkumar S. Shah

引用次数: 0

Integration of a derivatization protocol and LC–MS sequential window acquisition of all theoretical mass spectra strategy for amino acid determination in human plasma 整合衍生化协议和LC-MS顺序窗口获取所有理论质谱策略，用于测定人血浆中的氨基酸

SEPARATION SCIENCE PLUS Pub Date : 2023-09-13 DOI: 10.1002/sscp.202300100

Varvara Papaioannou, Eleni V. Mikropoulou, Amalia Yanni, Vaios Karathanos, Athina Aidini, Alexandre Paccou, Maria Halabalaki

引用次数: 0

A rapid and sensitive ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of atorvastatin calcium and lisinopril in rat plasma and its application in a pharmacokinetic study 一种快速灵敏的超高效液相色谱-串联质谱同时测定大鼠血浆中阿托伐他汀钙和赖诺普利的方法及其在药动学研究中的应用

SEPARATION SCIENCE PLUS Pub Date : 2023-09-11 DOI: 10.1002/sscp.202300129

Jirun Jia, Rui Bao, Jiayue Hou, Mengdi Qin, Wen Li, Li Yang, Qiang Fu

{"title":"A rapid and sensitive ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of atorvastatin calcium and lisinopril in rat plasma and its application in a pharmacokinetic study","authors":"Jirun Jia, Rui Bao, Jiayue Hou, Mengdi Qin, Wen Li, Li Yang, Qiang Fu","doi":"10.1002/sscp.202300129","DOIUrl":"https://doi.org/10.1002/sscp.202300129","url":null,"abstract":"Abstract Atorvastatin calcium and lisinopril are commonly used to reduce cholesterol and control blood pressure in the clinic. In this study, a sensitive and rapid method was developed and validated for the simultaneous detection of plasma concentrations of atorvastatin calcium and lisinopril using an ultra‐high performance liquid chromatography‐tandem mass spectrometry with an ACQUITY UPLC BEH C 18 column. The mobile phase of methanol and 0.1% formic acid aqueous solution was pumped at a flow rate of 0.2 mL/min. The retention times of atorvastatin calcium and lisinopril were 2.48 min and 1.99 min, respectively. The linear range of atorvastatin calcium in plasma samples was 1–2000 ng/mL, while that of lisinopril was 5–2000 ng/mL. The absolute values of intraday and inter‐day precision and accuracy were all below 15%. Furthermore, the recovery and matrix effects of atorvastatin calcium, lisinopril, and nimodipine were 80%–120% and 85%‐115%, respectively. Therefore, the developed method can be applied for the simultaneous quantification of atorvastatin calcium and lisinopril in rat plasma. The C max were about 199 ng/mL and 2059 ng/mL for atorvastatin calcium and lisinopril, respectively. In addition, the T 1/2 of atorvastatin calcium and lisinopril were 0.6 ± 0.3 and 5.4 ± 1.7 h, respectively.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136024312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High‐performance liquid chromatography‐based assay optimization for the detection of plasma amino acids for applications in metabolic disorders in developing countries 基于高效液相色谱法的血浆氨基酸检测优化，用于发展中国家代谢紊乱的应用

SEPARATION SCIENCE PLUS Pub Date : 2023-09-07 DOI: 10.1002/sscp.202300119

Muhammad Wasim, Haq Nawaz Khan, Abdul Tawab, Fazal e Habib, Mazhar Iqbal, Fazli Rabbi Awan

{"title":"High‐performance liquid chromatography‐based assay optimization for the detection of plasma amino acids for applications in metabolic disorders in developing countries","authors":"Muhammad Wasim, Haq Nawaz Khan, Abdul Tawab, Fazal e Habib, Mazhar Iqbal, Fazli Rabbi Awan","doi":"10.1002/sscp.202300119","DOIUrl":"https://doi.org/10.1002/sscp.202300119","url":null,"abstract":"Abstract Plasma amino acids are generally analyzed through ion exchange chromatography, a reproducible but time‐consuming method. Here, we report the optimization of a reverse‐phase‐high‐performance liquid chromatography with fluorescence detector (RP‐HPLC‐FLD) assay for the detection and quantification of plasma amino acids for potential applications in metabolic disorders (e.g., aminoacidopathies, a rare group of Inborn Errors of Metabolism). For assay development, initially standard amino acids were derivatized with ortho‐phthalaldehyde‐3‐mercaptopropionic acid (OPA‐3‐MPA) and filtered through a 0.20 μm syringe filter. The excitation and emission wavelengths of 240–450 nm (λex—λem) were used for the detection of amino acids. Chromatographic separation was achieved by gradient RP‐HPLC‐FLD through C18 symmetry column (150 × 4.6 mm, particle size 3.5 μm). HPLC assay was successfully optimized and was able to detect amino acids in the range of 10–400 ng/mL and good linearity (R 2 > 0.98) was achieved in the mixture for each standard amino acid. Moreover, the current assay showed great efficiency with two additional advantages: the use of low‐cost mobile phases, and the detection and quantification of amino acids at low level (ng/mL) concentration in biofluids. This assay could be applied for the analysis of human plasma to identify aminoacidopathies in newborn screening programs, and other metabolic disorders.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136365143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Robust method operable design region for economical and eco‐friendly chromatographic analysis of azilsartan medoxomil and cilnidipine by incorporating a hybrid approach of green analytical chemistry and analytical quality by design 采用绿色分析化学和分析质量设计相结合的方法，建立阿齐沙坦美多索米和西尼地平经济环保色谱分析的可操作设计区域

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-09-05 DOI: 10.1002/sscp.202300111

Pintu B. Prajapati, Abhinandan Shahi, Aneri Acharya, V. Pulusu, Shailesh Shah

{"title":"Robust method operable design region for economical and eco‐friendly chromatographic analysis of azilsartan medoxomil and cilnidipine by incorporating a hybrid approach of green analytical chemistry and analytical quality by design","authors":"Pintu B. Prajapati, Abhinandan Shahi, Aneri Acharya, V. Pulusu, Shailesh Shah","doi":"10.1002/sscp.202300111","DOIUrl":"https://doi.org/10.1002/sscp.202300111","url":null,"abstract":"According to the concept of green analytical chemistry, the analytical method development should be carried out by avoiding or minimizing the usage of toxic organic solvents for the safety of human and aquatic animal life and the protection of the environment. Hence, the green analytical chemistry (GAC)‐assisted robust liquid chromatographic method has been developed for chromatographic analysis of azilsartan medoxomil and cilnidipine (CIL) using safe organic solvents. The chromatographic method was developed by the implementation of analytical quality by design using chemometrics and response surface modeling. The chromatographic separation was carried out using Shim‐Pack C18 (250 × 4.6 mm, 5.0 μm) column as stationary phase and ethanol: 0.1% V/V ammonia solution (45:55, %V/V) as mobile phase. The chromatographic peak of AZL and CIL was found to be at the retention time of 3.5 and 4.5 min, respectively. The flow rate was kept at 1.0 mL/min and the column oven temperature was set to 40˚C. The developed method was found to be validated as per the International Council for Harmonization Q2 (R1) guideline. The method was applied for the assay of fixed‐dose combination and results were found in compliance with the labeled claim. The greenness of the method was evaluated using GAC tools.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46419719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of stability indicating thin‐layer chromatography method for simultaneous estimation of Benidipine hydrochloride and Telmisartan 同时测定盐酸苯尼地平和替米沙坦含量的薄层色谱法的建立和稳定性验证

SEPARATION SCIENCE PLUS Pub Date : 2023-08-31 DOI: 10.1002/sscp.202300075

Laxmibharti B. P. Mandal, Ketan Shah

{"title":"Development and validation of stability indicating thin‐layer chromatography method for simultaneous estimation of Benidipine hydrochloride and Telmisartan","authors":"Laxmibharti B. P. Mandal, Ketan Shah","doi":"10.1002/sscp.202300075","DOIUrl":"https://doi.org/10.1002/sscp.202300075","url":null,"abstract":"Abstract For the simultaneous estimation of Benidipine hydrochloride (BEN) and Telmisartan (TEL) in combined pharmaceutical dosage form, a stability‐indicating high‐performance thin‐layer chromatography (HPTLC) method has been developed and validated. It involves the use of HPTLC plates pre‐coated with silica gel 60F 254 and a mobile phase comprising of Methanol: Acetonitrile (1:9 v/v). The measurements of both drugs were performed at 237 nm. The drugs were subjected to acid‐alkali hydrolysis, photolytic, thermal, and oxidation degradation. The linearity of BEN was found to be between 200–1000 ng/band (R 2 = 0.9917), while for TEL, linearity was found to be between 2000 and 10000 ng/band (R 2 = 0.9979). The limit of detection and limit of quantitation were found to be 3.28 and 9.94 ng/spot for BEN and 158.79 and 481.17 ng/spot for TEL. The % relative standard deviation of the precision study was determined to be less than 2%. Various system suitability parameters were determined. The R f values of BEN and TEL were found to be 0.890 ± 0.0105 and 0.173 ± 0.0151, respectively. The resolution between BEN and TEL peak were found to be 4.48 and capacity factors were found to be 0.21 and 0.74 for BEN and TEL, respectively.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135891007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A validated high‐performance liquid chromatography method for the determination of brassinin, an indoleamine 2,3‐dioxygenase inhibitor in rat plasma 建立了一种高效液相色谱法测定大鼠血浆中吲哚胺2,3 -双加氧酶抑制剂花青素的方法

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-20 DOI: 10.1002/sscp.202300073

Pooja Dhurjad, Kajal Gupta, Akash P. Sakla, N. Shankaraiah, R. Sonti

{"title":"A validated high‐performance liquid chromatography method for the determination of brassinin, an indoleamine 2,3‐dioxygenase inhibitor in rat plasma","authors":"Pooja Dhurjad, Kajal Gupta, Akash P. Sakla, N. Shankaraiah, R. Sonti","doi":"10.1002/sscp.202300073","DOIUrl":"https://doi.org/10.1002/sscp.202300073","url":null,"abstract":"Brassinin, a phytochemical in cruciferous vegetables, is an indoleamine 2,3‐dioxygenase inhibitor and possesses a therapeutic opportunity in cancer immunotherapy. Here, we have developed and validated a novel, specific, and rapid bioanalytical method in rat plasma that is unavailable in the literature for preclinical studies. The method development involved single‐step protein precipitation as an extraction method using acetonitrile with an absolute and relative mean recovery of 79.32% and 93.59%, respectively. The method is accurate and precise over the linearity range of 1.0–50.0 μg/mL with a retention time of 5.3 and 3.5 min of brassinin and telmisartan, an internal standard. The intra‐day and inter‐day precision of the method expressed as a %coefficient of variation, ranging from 0.03% to 2.82% and 0.39% to 5.82%, whereas intra‐day and inter‐day accuracy ranged from 99.75% to 103.40% and 99.76% to 104.25%, respectively. The high‐performance liquid chromatography bioanalytical method was successfully validated as per Food and Drug Administration guidelines, and it can be applied in routine bioanalysis and pharmacokinetic studies.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46907907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of a high‐performance liquid chromatography method for detecting flubendazole and 2‐aminoflubendazole in canine plasma 高效液相色谱法检测犬血浆中氟苯达唑和2 -氨基氟苯达唑的验证

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-16 DOI: 10.1002/sscp.202300065

Joan Bergman, Lainey Harvill, Joe S. Smith, Ellen Haynes, Christopher A. Cleveland, M. Yabsley, S. Coker, Wided Najahi-Missaoui, D. Elder, S. Cox

引用次数: 1

A comprehensive review on recent trends in amino acids detection through analytical techniques 分析技术检测氨基酸的最新趋势综述

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-15 DOI: 10.1002/sscp.202300040

J. Kaur, N. Rangra, P. Chawla

{"title":"A comprehensive review on recent trends in amino acids detection through analytical techniques","authors":"J. Kaur, N. Rangra, P. Chawla","doi":"10.1002/sscp.202300040","DOIUrl":"https://doi.org/10.1002/sscp.202300040","url":null,"abstract":"The process of growth, development, and reproduction cannot take place in a live body without the presence of amino acids (AAs) since they are necessary for appropriate functioning. Detection of AAs in pharmaceutical and food samples is required to ensure quality, quantity, and efficacy. The AAs generally do not contain chromophores; hence their chromatographic analysis requires derivatization. The research papers published in the last few years were used to compile this manuscript. This manuscript covers various analytical, bioanalytical, and electrochemical approaches used in the identification of AAs. The analytical techniques like high‐performance liquid chromatography, ultra‐performance liquid chromatography, etc., and the hyphenated analytical techniques like liquid chromatography‐mass spectrometry, ultra‐performance liquid chromatography‐electrospray tandem mass spectrometry, and hydrophilic interaction ultra‐performance liquid chromatography are also discussed. This manuscript also briefly outlines the electrochemical analytical techniques including sensors and biosensors. This review article will be useful to the researcher working in the area of the development of analytical techniques for the detection of AAs.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41511657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances of hyphenated technique in impurity profiling of active pharmaceutical ingredients and pharmaceutical products 联用技术在活性药物成分和药品杂质分析中的研究进展

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-15 DOI: 10.1002/sscp.202300018

Nikhil Khandale, Rahul R. Rajge, Sachin Kumar Singh, Gurdeep Singh

{"title":"Advances of hyphenated technique in impurity profiling of active pharmaceutical ingredients and pharmaceutical products","authors":"Nikhil Khandale, Rahul R. Rajge, Sachin Kumar Singh, Gurdeep Singh","doi":"10.1002/sscp.202300018","DOIUrl":"https://doi.org/10.1002/sscp.202300018","url":null,"abstract":"Impurities found in active pharmaceutical ingredients (APIs) and pharmaceutical products are of ever‐increasing interest. According to several regulatory agencies, purity and impurity profiles are essential. An impurity is defined as any additional inorganic or organic material, residual solvents other than the medicinal components, or undesired compounds that remain with APIs. Impurities and degradation products in bulk drug materials and pharmaceutical formulations are identified, their structures are clarified, and their quantitative determination is part of impurity profiling. Unrecognized, poisonous impurities are dangerous to health and should be identified by selective procedures to increase the safety of drug therapy, and impurity profiling has become more significant in pharmaceutical analysis. This review briefly introduces process and product‐related impurities and emphasizes the creation of cutting‐edge analytical techniques for identifying them. It discusses the use of analytical methods, particularly high‐performance thin‐layer chromatography, liquid chromatography with mass spectrometry (MS), ultrahigh‐performance liquid chromatography, gas chromatography–MS, and nuclear magnetic resonance spectroscopy for the identification of contaminants and degradation products. It has discussed the importance of the quality, efficacy, and safety of drug substances and products, including the origin, types, and quality control of impurities, the need for the development of impurity profiling methods, impurity identification, and regulatory aspects.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45765414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0